Review
Oncology
Christopher McCann, Emma M. Kerr
Summary: Tumour cell metabolism plays a crucial role in promoting cancer cell survival, evasion of cell death, and development of therapeutic resistance. Targeting therapeutically-imposed metabolic dependencies can potentially revert metabolic reprogramming and enhance treatment efficacy.
Review
Oncology
Yu-Ling Bin, Hong-Sai Hu, Feng Tian, Zhen-Hua Wen, Mei-Feng Yang, Ben-Hua Wu, Li-Sheng Wang, Jun Yao, De-Feng Li
Summary: This review summarizes the impact of metabolic reprogramming on the tumor microenvironment and overcoming drug resistance in gastric cancer, and discusses clinical studies on combination treatments with conventional chemotherapy or molecular targeted therapy.
FRONTIERS IN ONCOLOGY
(2022)
Review
Cell Biology
Macus Hao-Ran Bao, Carmen Chak-Lui Wong
Summary: Hypoxia, a hallmark of solid cancers like hepatocellular carcinoma, contributes to drug resistance through molecular mechanisms. This review focuses on HIF-mediated metabolic reprogramming in drug resistance in HCC and suggests combination therapies targeting hypoxia-induced metabolic enzymes to overcome resistance. Efforts to identify novel mechanisms to combat refractory hypoxic HCC are crucial for developing more effective treatment regimens.
Article
Oncology
Tong Yu, Archana Chandrabhan Jadhav, Jiabao B. Xu, Adrian L. L. Harris, Venugopal Nair, Wei E. E. Huang
Summary: Understanding the development of cancer resistance to therapies is crucial. Newcastle disease virus (NDV) is a promising oncolytic agent, but some colon cancer cells show resistance to NDV reinfection. By using Raman spectroscopic and stable isotopic techniques, we found that the resistant cells slow down their replication and divert energy to protein and lipid synthesis. Understanding metabolic reprogramming could aid in developing precision cancer treatments that target resistant cells at the single-cell level.
Review
Oncology
Linlin Lv, Shilei Yang, Yanna Zhu, Xiaohan Zhai, Shuai Li, Xufeng Tao, Deshi Dong
Summary: This review analyzes and investigates the reprogramming of metabolism and resistance to breast cancer therapy, and the results offer promise for novel targeted and cell-based therapies.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Francesco Lasorsa, Nicola Antonio di Meo, Monica Rutigliano, Matteo Ferro, Daniela Terracciano, Octavian Sabin Tataru, Michele Battaglia, Pasquale Ditonno, Giuseppe Lucarelli
Summary: Prostate cancer is a common malignancy in men and a leading cause of cancer death. The cancer cells have specific metabolic characteristics, including increased lipogenesis, cholesterogenesis, and reliance on glutamine. The tumor microenvironment also plays a role in cancer progression through interactions between cancer and stromal cells. The use of multi-omic approaches can provide a comprehensive understanding of the metabolic changes in prostate cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Larischa de Wet, Anthony Williams, Marc Gillard, Steven Kregel, Sophia Lamperis, Lisa C. Gutgesell, Jordan E. Vellky, Ryan Brown, Kelly Conger, Gladell P. Paner, Heng Wang, Elizabeth E. Platz, Angelo M. De Marzo, Ping Mu, Jonathan L. Coloff, Russell Z. Szmulewitz, Donald J. Vander Griend
Summary: This study investigated the impact of SOX2 expression on patient outcomes and its function within prostate cancer cells. The results revealed that SOX2 expression promotes metastatic progression and therapy resistance in prostate cancer, and affects the metabolic pathways and metabolites of cancer cells. These findings contribute to a better understanding of the role of SOX2 in prostate cancer and suggest its potential as a biomarker and pharmacologic target in clinical settings.
Article
Immunology
Maosen Huang, Yancen Wu, Linyao Cheng, Lihua Fu, Haochao Yan, Haiming Ru, Xianwei Mo, Linhai Yan, Zijie Su
Summary: This study aimed to investigate the metabolic signature of primary tumor and circulating tumor cells in metastatic colorectal cancer (mCRC) patients using integrated omics analysis. The results showed that glucose uptake in tumor tissues was correlated with serum levels of ALT, TBIL, and CRE. Proteomics analysis revealed differentially expressed proteins in both glucose metabolic reprogramming (GMR) and epithelial-mesenchymal transition (EMT) pathways. Novel proteomic markers and potential therapeutic targets were identified, and a new index (CEA/blood glucose ratio) for diagnosing liver metastasis of colorectal cancer was proposed.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Ziyou Lin, Jingwei Li, Jian Zhang, Weineng Feng, Jiaye Lu, Xiaofan Ma, Wen Ding, Shumin Ouyang, Jinjian Lu, Peibin Yue, Guohui Wan, Peiqing Liu, Xiaolei Zhang
Summary: Acquired resistance is a bottleneck for targeted therapy in lung cancer, and metabolic adaptation may contribute to it. This study discovered a novel mechanism of acquired resistance to EGFR tyrosine kinase inhibitors mediated by IGF2BP3-dependent cross-talk between epigenetic modifications and metabolic reprogramming. IGF2BP3 upregulation is associated with reduced overall survival in TKI-resistant non-small cell lung cancer patients.
Review
Biochemistry & Molecular Biology
Liufeng Zhang, Yuancheng Wei, Shengtao Yuan, Li Sun
Summary: Abnormal energy metabolism is a characteristic of tumor cells, and mitochondria are important components of tumor metabolic reprogramming. Based on the concept of reprogramming mitochondrial metabolism, we discuss the current progress in mitochondrial metabolic reprogramming and summarized the corresponding treatment options. Finally, we propose mitochondrial inner membrane transporters as new and feasible therapeutic targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Rossella Delle Donne, Rosa Iannucci, Laura Rinaldi, Luca Roberto, Maria A. Oliva, Emanuela Senatore, Domenica Borzacchiello, Luca Lignitto, Giorgio Giurato, Francesca Rizzo, Assunta Sellitto, Francesco Chiuso, Salvatore Castaldo, Giovanni Scala, Virginia Campani, Valeria Nele, Giuseppe De Rosa, Chiara D'Ambrosio, Corrado Garbi, Andrea Scaloni, Alessandro Weisz, Concetta Ambrosino, Antonella Arcella, Antonio Feliciello
Summary: The study identifies praja2 as an essential regulator of cancer cell metabolism in glioblastoma (GBM) and a potential therapeutic target to suppress GBM growth. Silencing praja2 using siRNA delivered by nanoparticles reduces tumor size in GBM-bearing mice.
COMMUNICATIONS BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Baoyi Liu, Xin Zhang
Summary: The development of brain metastasis in breast cancer patients is a significant contributor to death, with limited effective treatment options available. Metabolic changes associated with cellular metastasis have been observed in primary breast cancer or brain metastases, highlighting the importance of understanding metabolic characteristics at each step of the metastatic cascade for developing efficient treatments.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Review
Oncology
Fahim Ahmad, Murali Krishna Cherukuri, Peter L. Choyke
Summary: While low-risk localized prostate cancer has a good prognosis with effective treatments, metastatic prostate cancer remains incurable and treatment resistance is a major issue. Resistance is primarily driven by tumor heterogeneity, altered genetic signatures, and metabolic reprogramming enabling tumors to adapt to drugs. Understanding prostate cancer metabolism may offer insights into therapy-induced adaptive responses and more durable therapeutic outcomes.
BRITISH JOURNAL OF CANCER
(2021)
Review
Biochemistry & Molecular Biology
Barbara Polonia, Cristina P. R. Xavier, Joanna Kopecka, Chiara Riganti, M. Helena Vasconcelos
Summary: In order to adapt to higher proliferation and energy demands, cancer cells undergo metabolic rewiring, particularly in lipid metabolism. Some of these metabolic alterations induce drug resistance in cancer cells. Extracellular Vesicles (EVs) play a crucial role in promoting tumor progression, survival and drug resistance by modulating cancer cell metabolism. This review focuses on discussing the importance of metabolic reprograming in cancer, specifically in glycolytic and lipid alterations, and highlights the significance of EVs in intercellular communication during this process.
CYTOKINE & GROWTH FACTOR REVIEWS
(2023)
Review
Oncology
Bin Xiong, Qiaoyi Huang, Huida Zheng, Shu Lin, Jianhua Xu
Summary: Colorectal cancer (CRC) is the most common and deadly cancer, and gene expression alteration is its main pathophysiological mechanism, leading to disrupted signaling pathways and cellular metabolic processes. MicroRNAs are closely related to CRC metabolism, proliferation, and chemotherapy resistance. Metabolic reprogramming is strongly associated with the development and prognosis of CRC, and microRNAs play a regulatory role by targeting enzymes involved in metabolic processes.
FRONTIERS IN ONCOLOGY
(2023)
Article
Instruments & Instrumentation
Tatu Rojalin, Dexter Antonio, Ambarish Kulkarni, Randy P. Carney
Summary: SERS is a powerful technique for sensitive label-free analysis, but automation progress is limited by manual sample preparation. Current automation efforts focus on downstream processing of collected data, but there is a need to develop algorithms to simplify and accelerate data collection. Machine learning-assisted methods at the acquisition stage can serve as a useful building block for point-of-care SERS diagnostic platforms.
APPLIED SPECTROSCOPY
(2022)
Article
Biotechnology & Applied Microbiology
Rachel R. Mizenko, Terza Brostoff, Tatu Rojalin, Hanna J. Koster, Hila S. Swindell, Gary S. Leiserowitz, Aijun Wang, Randy P. Carney
Summary: The study revealed distinct patterns in tetraspanin expression of EVs from different sources, with flow cytometry measurements showing some inconsistencies. Non-specific tetraspanin capture can bias multiplexed tetraspanin profiles, impacting diagnostic sensitivity for tumor-associated EV surface markers. The findings provide insights into protein expression heterogeneity of EVs, informing better EV capture and detection platforms for diagnostic or downstream use.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Zhiyi Yang, Wenbo Li, Hao Huang, Songlei Ren, Yongfan Men, Fang Li, Xuefeng Yu, Qian Luo
Summary: In this study, black phosphorus nanomaterial was utilized as the matrix for MALDI-MS, combined with microchannel technique, to effectively detect a variety of lipids with high sensitivity and reproducibility, and high salt tolerance. The method was successfully applied in detecting serum phospholipids in children patients with asthma or bronchopneumonia, providing new insights into the development of rapid screening and identification of disease indicators in biological fluids.
Article
Chemistry, Multidisciplinary
Silvia Hilt, Ruiwu Liu, Izumi Maezawa, Tatu Rojalin, Hnin H. Aung, Madhu Budamagunta, Ryan Slez, Qizhi Gong, Randy P. Carney, John C. Voss
Summary: This study synthesized a class of bifunctional stilbenes to modulate the toxicity and oxidative stress of amyloid beta oligomers (AβO) in Alzheimer's disease. The findings suggest that this bifunctional approach has the potential to counteract the molecular pathogenesis of Alzheimer's disease in a powerful and synergistic manner.
FRONTIERS IN CHEMISTRY
(2022)
Article
Chemistry, Physical
Hanna J. Koster, Tatu Rojalin, Randy P. Carney, Hannah J. O'Toole, Kwan Lun Chiu
Summary: Surface enhanced Raman scattering (SERS) is a powerful tool for vibrational spectroscopy that enhances chemical sensitivity by several orders of magnitude compared to spontaneous Raman scattering. However, synthesizing robust and uniform SERS substrates quickly and easily remains a challenge.
COLLOID AND INTERFACE SCIENCE COMMUNICATIONS
(2022)
Review
Multidisciplinary Sciences
Jiannan Li, Carolyn Kim, Chi-Chun Pan, Aaron Babian, Elaine Lui, Jeffrey L. Young, Seyedsina Moeinzadeh, Sungwoo Kim, Yunzhi Peter Yang
Summary: This review presents bioprinting methods, biomaterials, and printing strategies for composite tissue constructs for musculoskeletal applications, with a focus on the new strategy of integrating different components through hybrid bioprinting.
Article
Microbiology
Rachel R. Mizenko, Terza Brostoff, Kenneth Jackson, Patricia A. Pesavento, Randy P. Carney
Summary: Feline calicivirus (FCV) is a significant cause of respiratory disease in cats and has implications for human medical research. This study explores the role of extracellular vesicles (EVs) in FCV infection and highlights the importance of coisolates during viral purification.
MICROBIOLOGY SPECTRUM
(2022)
Review
Nanoscience & Nanotechnology
Jugal Suthar, Marissa Taub, Randy P. Carney, Gareth R. Williams, Stefan Guldin
Summary: Extracellular vesicles (EVs) are widely regarded as a valuable source of biomarkers for human health and disease. However, their small size and complexity present challenges for effective characterization. This has led to the development of novel EV biosensing techniques to overcome these limitations.
WILEY INTERDISCIPLINARY REVIEWS-NANOMEDICINE AND NANOBIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Xiannian Zhang, Zhenwei Qian, Mengcheng Jiang, Wentao Li, Yanyi Huang, Yongfan Men
Summary: A highly efficient passive mixing device based on a split-and-recombine (SAR) configuration was developed for rapid and nearly complete mixing performance. The structure parameters of the micromixer were optimized through numerical simulations to enhance mixing efficiency. The mixing mechanism involved three synergetic effects: rotation, combination, and stretching, with pressure gradients inducing vertical convection to accelerate the mixing process.
Article
Nanoscience & Nanotechnology
Lalithasri Ramasubramanian, Harsha Jyothi, Leora Goldbloom-Helzner, Brandon M. Light, Priyadarsini Kumar, Randy P. Carney, Diana L. Farmer, Aijun Wang
Summary: This study validates lipid rafts as a functional biomaterial for synthesizing biomimetic nanovesicles and shows their potential in promoting neurogenesis and angiogenesis.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Engineering, Biomedical
Jiannan Li, Seyedsina Moeinzadeh, Carolyn Kim, Chi -Chun Pan, George Weale, Sungwoo Kim, Geoffrey Abrams, Aaron W. James, HyeRan Choo, Charles Chan, Yunzhi Peter Yang
Summary: This paper presents the development of a room temperature printable hydrogel bioink by introducing PEGDMA and xanthan gum into the GelMA/alginate system. The newly developed hydrogel system demonstrates extrusion printability, tunable mechanical property and degradation, ionic solubility, and cytocompatibility.
Article
Multidisciplinary Sciences
Hanna J. Koster, Antonio Guillen-Perez, Juan Sebastian Gomez-Diaz, Maria Navas-Moreno, Andrew C. Birkeland, Randy P. Carney
Summary: Raman spectroscopy shows great promise for liquid biopsy cancer detection and diagnosis as it is rapid, label-free, non-destructive, and requires little to no sample preparation. By fusing the Raman spectra of plasma and saliva, higher diagnostic performance can be achieved, demonstrating the ability of Raman spectroscopy to provide additive value across different biofluid sources.
SCIENTIFIC REPORTS
(2022)
Article
Medicine, Research & Experimental
Dake Hao, Lu Lu, Hengyue Song, Yixin Duan, Jianing Chen, Randy Carney, Jian Jian Li, Ping Zhou, Jan Nolta, Kit S. Lam, J. Kent Leach, Diana L. Farmer, Alyssa Panitch, Aijun Wang
Summary: A new generation of collagen-binding extracellular vesicles (EVs) was developed to improve their retention and therapeutic efficacy. The results showed that these collagen-binding EVs had longer in situ retention, suppressed inflammatory responses, and enhanced tissue regeneration.
Article
Chemistry, Multidisciplinary
Hanna J. Koster, Tatu Rojalin, Alyssa Powell, Dina Pham, Rachel R. Mizenko, Andrew C. Birkeland, Randy P. Carney
Summary: This study confirms that common EV isolation methods yield variable lipoprotein content, and dual isolation methods are necessary for successful separation of EVs from different lipoprotein classes. Additionally, disease state is the main driver for distinguishing EV samples, largely unaffected by the choice of isolation method.
Article
Chemistry, Multidisciplinary
Meruyert Imanbekova, Sorina Suarasan, Tatu Rojalin, Rachel R. Mizenko, Silvia Hilt, Meghna Mathur, Paula Lepine, Michael Nicouleau, Nguyen-Vi Mohamed, Thomas M. Durcan, Randy P. Carney, John C. Voss, Sebastian Wachsmann-Hogiu
Summary: This study used Raman spectroscopy to analyze the biochemical composition of Aβ peptide in extracellular vesicles (EVs) associated with Alzheimer's disease. The results suggest that Aβ associated with secreted EVs has an alpha-helical secondary structure and the size of a monomer or small oligomer. Additionally, altered fatty acid chain lengths were identified in EVs carrying Aβ, potentially affecting the fluidity of the EV membrane.
NANOSCALE ADVANCES
(2021)