Article
Materials Science, Multidisciplinary
Zachary Hallenbeck, Esther A. Wertz
Summary: This work introduces a new technique that combines super-resolution microscopy with fluorescence lifetime imaging microscopy to study the enhancement of single-molecule decay rate by plasmonic nanoparticles. The technique provides high spatial resolution and temporal resolution, allowing for a better understanding of the mechanisms of plasmon-enhanced emission.
ADVANCED OPTICAL MATERIALS
(2022)
Article
Biotechnology & Applied Microbiology
Weisong Zhao, Shiqun Zhao, Liuju Li, Xiaoshuai Huang, Shijia Xing, Yulin Zhang, Guohua Qiu, Zhenqian Han, Yingxu Shang, De-En Sun, Chunyan Shan, Runlong Wu, Lusheng Gu, Shuwen Zhang, Riwang Chen, Jian Xiao, Yanquan Mo, Jianyong Wang, Wei Ji, Xing Chen, Baoquan Ding, Yanmei Liu, Heng Mao, Bao-Liang Song, Jiubin Tan, Jian Liu, Haoyu Li, Liangyi Chen
Summary: Sparse structured illumination microscopy (Sparse-SIM) achieves nearly twofold resolution enhancement by utilizing sparse deconvolution algorithm, enabling the resolution of intricate biological structures such as small fusion pores, nuclear pores, and relative movements of inner and outer mitochondrial membranes.
NATURE BIOTECHNOLOGY
(2022)
Article
Optics
Prakash Joshi, Partha Pratim Mondal
Summary: Molecular assembly in cellular environments is crucial for understanding biological mechanisms. Biophysical parameters directly linked to cellular states require a multimodel approach for accurate estimation.
Article
Optics
Lixin Liu, Meijie Qi, Yujie Liu, Xinzhu Xue, Danni Chen, Junle Qu
Summary: Fluorescence imaging is important in cell biology and biomedical research. Single-molecule localization microscopy is a super-resolution imaging technique that can efficiently locate sparse fluorescent molecules. A single-molecule localization algorithm based on blind deconvolution and centroid localization method is proposed in this paper, which can effectively reconstruct images and locate fluorescent molecules.
Review
Biochemistry & Molecular Biology
Charles Bond, Adriana N. Santiago-Ruiz, Qing Tang, Melike Lakadamyali
Summary: Since its initial demonstration in 2000, there have been significant technological advancements in far-field super-resolution light microscopy. These developments have allowed for unprecedented visualization of the inner workings of cells. This review discusses the technical details of common implementations of super-resolution microscopy and highlights recent promising advances in the field.
Article
Biology
Jigmi Basumatary, Neptune Baro, Prakash Joshi, Partha Pratim Mondal
Summary: Single-molecule localization microscopy (SMLM) is a powerful technique that improves spatial resolution beyond the diffraction limit and allows for the analysis of biological features at the nanoscale. This study introduces a single molecule-based scanning SMLM (scanSMLM) system that enables rapid volume imaging. By rapidly changing the aperture of an electrically-tunable lens, the system achieves selective imaging of the axial object plane, allowing for volume scanning. The technique is employed to visualize the architecture of actin-filaments, the distribution of Meos-Tom20 molecules on the mitochondrial membrane, and the clustering of Hemagglutinin (HA) protein single molecules for studying Influenza-A disease progression. The system enables 3D visualization of HA distribution, providing unprecedented insights into the disease progression at the single-molecule level.
COMMUNICATIONS BIOLOGY
(2023)
Review
Cell Biology
Jianquan Xu, Yang Liu
Summary: Chromatin organization plays a crucial role in gene regulation and genome maintenance. Disruption of chromatin structure is associated with various pathological processes and diseases. Recent advances in single molecule localization microscopy (SMLM) provide new opportunities to visualize chromatin architecture at nanometer resolution and improve our understanding of diseases.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemical Research Methods
David J. Schodt, Farzin Farzam, Sheng Liu, Keith A. Lidke
Summary: We introduce the Sequential Super-resolution Microscope (SeqSRM), a dedicated microscope for automated sequential localization microscopy. SeqSRM automates precise stage stabilization and data acquisition of user-selected cells, minimizing user interaction. We demonstrate that nanometer-scale changes in cell morphology can impact the fidelity of multi-target super-resolution reconstructions, but these shifts can be reliably detected and masked out using brightfield images. SeqSRM enables automated multi-target imaging without the need for multiple fluorophores, while accurately reflecting the relative organization of the targets.
BIOMEDICAL OPTICS EXPRESS
(2023)
Review
Chemistry, Analytical
Hua Liu, Zhongju Ye, Yanan Deng, Jie Yuan, Lin Wei, Lehui Xiao
Summary: This review describes the basic principles of single-molecule localization microscopy (SMLM), factors affecting imaging quality, recent achievements in the development of fluorescent probes for SMLM, and challenges and future advances in SMLM for biological applications.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Food Science & Technology
Susana Dianey Gallegos-Cerda, Josue David Hernandez-Varela, Jose Jorge Chanona-Perez, Benjamin Arredondo Tamayo, Juan Vicente Mendez Mendez
Summary: Super-resolution microscopy has the potential to revolutionize food science by providing images beyond the diffraction limit of light microscopes. However, its application in this field is relatively unknown. This review provides a comprehensive overview of the techniques, advances, and challenges of super-resolution microscopy in food science.
FOOD AND BIOPROCESS TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Silvia Scalisi, Francesca Pennacchietti, Sandeep Keshavan, Nathan D. Derr, Alberto Diaspro, Dario Pisignano, Agnieszka Pierzynska-Mach, Silvia Dante, Francesca Cella Zanacchi
Summary: Single layer graphene (SLG) is an important nanomaterial with unique optical and electrical properties, making it a potential candidate for biomedical applications, biosensors, and tissue engineering. Studies have shown high cellular affinity for graphene, but the molecular mechanisms driving/regulating cellular adhesion and migration on SLG surfaces are not well understood. Research using super-resolution microscopy and cluster analysis has provided insights into the molecular distribution of adhesion proteins on SLG and revealed substrate-dependent interactions in different cell types.
Review
Nanoscience & Nanotechnology
A. Femius Koenderink, Roman Tsukanov, Joerg Enderlein, Ignacio Izeddin, Valentina Krachmalnicoff
Summary: This article summarizes the recent advances in the field of nanophotonics that have leveraged SMLM and shows how some concepts commonly used in nanophotonics can benefit the development of new microscopy techniques for biophysics. It introduces the basic concepts of SMLM and the observables that can be measured, linking them with their corresponding physical quantities of interest in biophysics and nanophotonics.
Article
Chemistry, Multidisciplinary
Shikha Dhiman, Teodora Andrian, Beatriz Santiago Gonzalez, Marrit M. E. Tholen, Yuyang Wang, Lorenzo Albertazzi
Summary: Characterization of newly synthesized materials is crucial in chemistry and nanotechnology, with standardized analytical techniques and novel technologies continually developing. The adoption of reporting standards like MIRIBEL aims to provide reproducible and reliable sample preparation for the scientific community. Super-resolution microscopy (SRM) shows potential to transition into a standard routine method for material characterization, but faces challenges in sample preparation, data analysis, and expensive instrumentation.
Article
Plant Sciences
Miroslav Ovecka, Jiri Sojka, Michaela Ticha, George Komis, Jasim Basheer, Cintia Marchetti, Olga Samajova, Lenka Kubenova, Jozef Samaj
Summary: The documentation of plant growth and development requires integrative and scalable approaches. Recent developments in mesoscopy, microscopy and nanoscopy methods have been applied to imaging of plant subcellular compartments, cells, tissues, and organs. However, the current LSFM and SRM modalities have limitations and there is a trend towards designing integrated microscopic modalities for broader multiscale plant imaging.
Article
Biochemistry & Molecular Biology
Minsu Kang, Jooyong Lee, Sangyoon Ko, Sang-Hee Shim
Summary: The study introduced a trifunctional streptavidin to link the target, fluorophore and gel matrix, improving labeling efficiency and minimizing linkage error in ExSMLM images. This method provides a simple and practical means for fluorescence imaging with molecular resolution.
Article
Chemistry, Multidisciplinary
Tonghui Wang, Jinbo Fei, Zhenzhen Dong, Xia Xu, Weiguang Dong, Junbai Li
Summary: In this study, a synthetic electrozyme was coupled with a supramolecule-assembled nanoarchitecture to mimic mitochondrial oxidative phosphorylation and achieve enhanced bioenergy transformation. The metal-free electrozyme, a semiconducting polymer deposited on an electrode, exhibited a well-matched electrocatalytic property that allowed the oxidation of reduced nicotinamide adenine dinucleotide (NADH) to release protons at a much lower electric potential. As a result, a proton gradient was generated and drove the rotary catalysis of adenosine 5'-triphosphate (ATP) synthase in a lipid membrane, resulting in the production of ATP. Significantly, the electrochemical bioenergy conversion of NADH to ATP in this bio-like system was much more efficient compared to natural mitochondria. This work integrates synthetic and natural catalytic chemistry, facilitating enhanced bioenergy transformation and greatly improving prospects in ATP-fueled bioapplications.
Review
Chemistry, Physical
Jinbo Fei, Junbai Li
Summary: Molecular assembly is a promising strategy for constructing active systems using biomolecules as building blocks. These assembled systems simulate or regulate important biological activities and have great potential in various bioapplications. This review focuses on recent progress in ATP-involved active self-assembled systems, which are constructed with hierarchical structures and generate ATP using external influences to create proton gradients. Additionally, active supermolecular systems driven by ATP as chemical fuel are highlighted. Finally, key challenges and future research perspectives are discussed.
CURRENT OPINION IN COLLOID & INTERFACE SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Xianbao Li, Qi Li, Aoli Wu, Junbai Li
Summary: Symmetry of layered dipeptide crystals can be broken by utilizing CO2, which induces adjacent monomolecular layers to stack in the same direction. CO2 covers the interlayer interaction sites, forcing the dipeptides to adsorb asymmetrically. The dipeptide crystals exhibit enhanced piezoelectricity and the piezoelectric voltage generated from dipeptide-based generators is increased by more than 500% after symmetry breaking. This work provides a potential route to engineer structures and properties of layered materials, and offers insights into the control of non-covalent interactions.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Physical
Aoli Wu, Yongxian Guo, Xianbao Li, Qi Li, Guixin Chen, Hengchang Zang, Junbai Li
Summary: The development of peptide-based supramolecular materials with hierarchical morphology and tunable guest loading shows great potential as a drug carrier due to their biocompatibility and biodegradability. In this study, a facile Schiff base nanoarchitectonic was reported for the supramolecular assembly of diphenylalanine (FF) metastable gel. The competitive assembly between FF-H2O and FF-GA oligomer resulted in the formation of FF nanoparticles and hierarchical beaded nanofibers. These assembled particles can easily load various guest molecules and release them under weak alkaline conditions, demonstrating their potential application as drug carriers.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Chemistry, Physical
Nan Sun, Yi Jia, Shiwei Bai, Qi Li, Luru Dai, Junbai Li
Summary: Super-resolution microscopy (SRM) technology has revolutionized the field of cell biology by breaking the diffraction limit, enabling researchers to visualize cellular structures with nanometric resolution, multiple colors, and single-molecule sensitivity. SRM has also extended its impact to other fields such as nanomedicine, material science, and nanotechnology, greatly contributing to breakthroughs in these areas.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2023)
Article
Chemistry, Physical
Xin Li, Honglei Jian, Qingquan Han, Anhe Wang, Jieling Li, Ningyuan Man, Qi Li, Shuo Bai, Junbai Li
Summary: Short peptide self-assembled hydrogels have excellent biocompatibility and functional expansion, making them promising in cell culture and tissue engineering. However, the preparation of bio-inks with adjustable mechanical strength and controlled degradation for 3D bioprinting is challenging.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Chemistry, Physical
Zhenzhen Dong, Jinbo Fei, Tonghui Wang, Junbai Li
Summary: A strategy of matched spectral and temporal light management is developed to improve photosynthetic efficiency by co-assembling natural thylakoid membrane (TM) with artificial long afterglow particle (LAP). LAP with excellent stability and biocompatibility is optically-matched with the absorption of TM, serving as an additional light source for photosynthesis. Enhanced photosynthesis is achieved after co-assembly, with boosted electron transfer, oxygen yield, and ATP production due to down-conversion fluorescence emission from LAP. This proof-of-concept work opens a new route to augment the photosynthetic efficiency of green plants by utilizing precise light-managed materials.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Chemistry, Physical
Yilin Liu, Junbai Li
Summary: This study reports, for the first time, that Celastrol can self-assemble into size-controllable nanoparticles via the anti-solvent method using different solvents or varying Celastrol concentrations. In vitro experiments showed that the prepared nanoparticles can effectively kill MCF-7 cells. Furthermore, the nanoparticles can accumulate efficiently in tumors after intravenous injection. Administration of a lethal dosage of Celastrol resulted in significant tumor suppression with maintained activity in mice. These findings suggest that the anti-solvent method holds promise for fabricating Celastrol nano-drugs with controlled size and reduced systemic toxicity for clinical cancer treatment.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Review
Chemistry, Multidisciplinary
Jie Zhao, Xia Xu, Yang Yang, Junbai Li
Summary: Photodynamic therapy (PDT) is an efficient method for cancer treatment, but traditional photosensitizers (PSs) often have low bioavailability. This review discusses the use of molecular-assembly technique, adjusting the PS triplet state lifetime, and developing aggregation-induced emission (AIE) agents to improve the efficacy of PDT.
Article
Chemistry, Multidisciplinary
Yilin Liu, Jie Zhao, Xia Xu, Yang Xu, Wei Cui, Yang Yang, Junbai Li
Summary: The natural anthraquinone derivative emodin (Emo) is demonstrated to be a promising photosensitizer for two-photon-excited photodynamic therapy with a large two-photon absorption cross-section and high singlet oxygen quantum yield. The co-assembled Emo/HSA nanoparticles exhibit outstanding TPE-PDT properties against cancer cells. This work is beneficial for the use of natural extracts for high-efficiency TPE-PDT.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Multidisciplinary
Zibo Li, Fanchen Yu, Xia Xu, Tonghui Wang, Jinbo Fei, Jingcheng Hao, Junbai Li
Summary: We demonstrate the realization of photozyme-catalyzed oxidative phosphorylation through the assembly of ATP synthase-reconstituted proteoliposome coatings on microcapsules. This is achieved through layer-by-layer deposition of semiconducting graphitic carbon nitride (g-C3N4) nanosheets and polyelectrolytes. The encapsulated g-C3N4 nanosheets act as the photozyme, accelerating the oxidation of glucose to yield protons under light illumination, establishing an outward transmembrane proton gradient to drive ATP synthesis. This artificial design enables higher energy conversion efficiency compared to conventional oxidative phosphorylation systems.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Materials Science, Multidisciplinary
Peng Zhou, Ruirui Xing, Qi Li, Junbai Li, Chengqian Yuan, Xuehai Yan
Summary: Controlling the morphology of supramolecular hydrogels is crucial in tuning their mechanical properties. This study investigates the evolution of fibrillar networks in hydrogels by modulating droplets formed via liquid-liquid phase separation. The size and amount of droplets, as well as the structural evolution and network development, can be finely tuned by varying the thermal history. The findings provide insights into the role of phase-separated droplets in self-assembling networks and contribute to the engineering of supramolecular hydrogels with adjustable mechanical properties.
Review
Chemistry, Multidisciplinary
Rui Chang, Luyang Zhao, Ruirui Xing, Junbai Li, Xuehai Yan
Summary: This review summarizes recent advances in chromopeptide nanoarchitectonics, focusing on the design strategy, assembly mechanism, and structure-function relationship. The effect of peptide sequences and the variation in photophysical performance are critically emphasized. Various applications, including biomedicine and artificial photosynthesis, are discussed, along with the future prospects of chromopeptide nanoarchitectonics.
CHEMICAL SOCIETY REVIEWS
(2023)
Article
Chemistry, Multidisciplinary
Aoli Wu, Yongxian Guo, Meiqi Li, Qin Li, Hengchang Zang, Junbai Li
Summary: The assembly pathway and chiral transformation behavior of peptides in supramolecular peptide assemblies remain largely unknown. In this study, the assembly of an N-terminal-protected phenylalanine-tyrosine dipeptide is shown to proceed through multistep structural evolution, and the chiral assembly can be controlled by introducing a bipyridine derivative and using ultrasound or enzyme catalysis.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Physical
Nan Sun, Yi Jia, Shiwei Bai, Yang Yang, Luru Dai, Junbai Li
Summary: This study demonstrates the direct observation and quantification of protein corona adsorbed onto PEGylated mesoporous silica particles using direct stochastic optical reconstruction microscopy. The effects of PEG molecular weights and incubation time on protein penetration depth are investigated for the first time.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2024)