Article
Environmental Sciences
Dou Ye, Qingxiu Hu, Xue Bai, Wenjie Zhang, Hongwei Guo
Summary: Due to regular annual harvest, Phragmites australis in wetland protection generates an abundance of straw waste. Therefore, finding a convenient and efficient way to utilize the excess straw is necessary. This study analyzed the use of P. australis straw in Pleurotus cultivation and spent mushroom substrate compost, showing positive economic benefits. The recycling pathway for P. australis resources presents ecological and social benefits and is a sustainable and eco-friendly solution for agricultural waste.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Ecology
Fabricio Rocha Vieira, John Andrew Pecchia
Summary: Unlike other fungal species, commercial Agaricus bisporus cultivation relies on ecological relationships with a broad range of microorganisms present in compost and casing. The bacterial community dynamics shift throughout the cultivation cycle, with compost, casing, and mushrooms representing different niches for bacteria. Despite this, there is a possibility of bacterial exchange between these niches.
Article
Environmental Sciences
Diego C. Zied, Carlos G. de Abreu, Lucas da S. Alves, Evandro P. Prado, Arturo Pardo-Gimenez, Paulo C. de Melo, Eustaquio S. Dias
Summary: The study indicates that using fresh spent mushroom substrate (SMS) in the production of lettuce and arugula is feasible, with different optimal dosages recommended based on evaluated agronomic parameters.
JOURNAL OF ENVIRONMENTAL MANAGEMENT
(2021)
Article
Horticulture
Daniel Hernandez, Margarita Ros, Francisco Carmona, Jose Antonio Saez-Tovar, Jose Antonio Pascual
Summary: The use of composts of spent mushrooms substrates as a partial replacement for peat in horticulture can lead to higher yields, with a combination of different types of compost showing increased suppressiveness against plant pathogens.
Article
Chemistry, Multidisciplinary
Hiroya Nakauchi, Yoshihiko Amano, Satomi Tagawa
Summary: A new method was developed to extract mycelial fibers from mushrooms without destroying their structure. The fruiting bodies were treated with NaOH and H2O2, and decolorized using sunlight irradiation. The resulting mycelium pulp is a completely new material that retains its mycelial structure, demonstrating excellent deformability and a wide range of material applicability.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Article
Food Science & Technology
Marcel Golian, Alzbeta Hegedusova, Ivana Mezeyova, Zuzana Chlebova, Ondrej Hegedus, Dana Urminska, Alena Vollmannova, Peter Chlebo
Summary: This study evaluates the accumulation ability of 59 oyster mushroom strains in terms of selected metals in the cap and stipe. The research reveals the differential accumulation of metals and confirms the dependence of metal uptake on substrate fortification. The findings can contribute to the selection of production strains with hyperaccumulation properties and have implications for food safety.
Article
Agriculture, Multidisciplinary
Jing Wu, Kazuki Uchida, Aoto Yoshikawa, Masaru Hashimoto, Mitsuru Kondo, Kenichi Nihei, Mizuki Ishii, Jae-Hoon Choi, Yukihiro Miwa, Chiho Shoda, Deokho Lee, Ayaka Nakai, Toshihide Kurihara, Corina N. D'Alessandro-Gabazza, Masaaki Toda, Taro Yasuma, Esteban C. Gabazza, Hirofumi Hirai, Hirokazu Kawagishi
Summary: Mushroom-forming fungi secrete liquid on mycelia surface before fruiting-body formation, which has been overlooked by mushroom researchers. We hypothesize that this liquid, called the fruiting liquid (FL), plays a crucial role in fruiting body formation and produces bioactive compounds. Four novel compounds (1-4) were isolated from FL of two mushroom species, with their structures determined through various methods. Compounds 1, 3, and 4 induced fruiting body formation in a different mushroom species, while compound 4 showed inhibitory effects on hypoxia-inducible factor and compounds 2-4 suppressed the expression of receptor tyrosine kinase (Axl).
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Zhen-Zhu Zhao, Fei Zhang, Bao-Yu Ji, Ning Zhou, Hui Chen, Yan-Jun Sun, Wei-Sheng Feng, Xiao-Ke Zheng
Summary: Nine pyrrole alkaloid derivatives, including four new ones, were isolated from Lentinula edodes for the first time. Their chemical structures were determined using various spectroscopic and crystallographic techniques. Compound 1, a previously unreported bicylo-pyrrole aldehyde homologue, was found to be a major component in the mushroom with cytotoxic activity against SMMC-772 cells. Compounds 1 and 2 displayed weak immunosuppressive activities, while compound 3 showed inhibition activity on the proliferation of HaCaT cell line and weak antioxidant activity.
Article
Biotechnology & Applied Microbiology
Shoji Ikeda, Masahito Yamauchi, Takahiro Watari, Masashi Hatamoto, Masayoshi Yamada, Shinya Maki, Hirofumi Hara, Takashi Yamaguchi
Summary: The study found that spent mushroom substrate-anaerobic digestion residue (SMS-ADR) can be used as a medium base material for Enokitake mushroom cultivation, showing potential for food production. SMS-ADR pretreated with KOH and NaOH performed well in mushroom cultivation, similar to the standard medium, while SMS not involved in the anaerobic digestion process showed inhibitory effects on mycelial growth for Enokitake mushrooms.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Environmental Sciences
Mi Na Rhie, Kai Hong, Taeyoon Lee
Summary: The study investigated the optimum conditions for maximizing mixotrophic cultivation of Scenedesmus acuminatus using acetate as an organic carbon source. Factors like aeration rate, pH control, and the type/concentration of acetate were found to significantly impact the growth of S. acuminatus. The importance of dissolved oxygen and pH control in the cultivation process was highlighted, with a particular emphasis on the use of ammonium acetate dosage for optimal growth.
ENVIRONMENTAL TECHNOLOGY
(2022)
Article
Microbiology
Bea-Ven Chang, Chiao-Po Yang, Chu-Wen Yang
Summary: The study shows that spent mushroom composts (SMCs) can effectively remove phthalates from the environment and urban wastewater through adsorption and biodegradation processes. Enzyme extracts from four edible mushrooms have been found to degrade phthalates such as benzyl butyl phthalate (BBP), di-n-butyl phthalate (DBP), and diethyl phthalate (DEP). Metaproteomic analysis using mass spectrometry revealed potential fungus enzymes associated with phthalate degradation in SMCs. These findings suggest that the application of SMCs for phthalate removal could be developed into a green and sustainable mycoremediation-based technology.
Article
Horticulture
Giuseppe Picca, Asier Goni-Urtiaga, Cristina Gomez-Ruano, Cesar Plaza, Marco Panettieri
Summary: This study investigated the agronomical performances of two spent coffee ground-based composts with and without biochar as peat replacement for tomato development. The results showed that mixing coffee ground compost with peat promoted an increase in fruit production. However, the effectiveness of this alternative solution depends on the dilution level and plant development stage.
Article
Horticulture
Wen-Shing Chen, Wen-Tien Tsai, Yu-Quan Lin, Chi-Hung Tsai, Yao-Tsung Chang
Summary: The edible mushroom industry has led to the generation of a significant amount of spent mushroom compost (SMC), which is considered as agricultural waste. In this study, SMCs from shiitake and black fungus cultivation were converted into porous biochar products through pyrolysis. The pore and chemical properties of the resulting biochar products were studied and correlated with the pyrolysis temperature. The research found that the pore properties of the biochar products increased with higher pyrolysis temperatures.
Article
Food Science & Technology
Yunting Li, Yao Feng, Yuling Shang, Heran Xu, Rongrong Xia, Zhenshan Hou, Song Pan, Li Li, Yuanyuan Bian, Jiayi Zhu, Zijian Wang, Guang Xin
Summary: Edible mushroom sexual spores have gained increasing interest due to their bioactive components and functions. This review summarizes the bioactive nutrients of these spores, describes the mechanism of spore discharge, and analyzes the relationship between postharvest spore discharge and mushroom quality. Spores contain various bioactive nutrients that are beneficial to human health. Mature mushrooms can actively discharge spores, which is influenced by light, relative humidity, and temperature. Storage leads to vigorous physiological metabolism of spore-bearing gill tissue, promoting postharvest spore release and altering the nutritional value of fruiting bodies. The flavor of the fruiting bodies also changes significantly during spore discharge. Edible mushroom sexual spores have the potential to be used as new raw materials for functional food and medical resources. Research on the effect of spore discharge on mushroom quality and the development of spore discharge regulation technology may be a new trend in quality control and promotion of the edible mushroom industry.
FOOD SCIENCE AND HUMAN WELLNESS
(2023)
Article
Microbiology
Yongsheng Ma, Lingyun Liu, Xiaoyan Zhou, Tian Tian, Shuai Xu, Dan Li, Changtian Li, Yu Li
Summary: This study investigated the composting of spent mushroom substrate produced from mushroom cultivation with different straw additions and using chicken manure. The results showed that the spent mushroom substrate produced by adding 20% straw was more suitable for composting treatment. The findings suggest that incorporating an appropriate amount of straw in mushroom cultivation facilitates subsequent composting of spent mushroom substrate, providing an effective strategy for environmental protection and cost reduction.
Article
Energy & Fuels
Dayana A. Rodrigues, Antonio J. Demuner, Luiz C. A. Barbosa, Gustavo A. M. Pereira, Jose D. Fabris, Felix G. de Siqueira, Marcio T. Pereira, Abelardo Silva Junior, Otavio V. Carvalho
Summary: Both pressing and solvent extraction of Jatropha curcas seed oil produce a large amount of cake byproduct, which contains the highly toxic phorbol esters (PEs) that threaten mammal health. Various treatments, including ammonium hydroxide, urea, heat, ultraviolet radiation, and gamma radiation, were tested for their effectiveness in degrading PEs. It was found that an aqueous ammonium hydroxide solution at 70 degrees C and 3% w/w reduced the PEs content in the cake and bran to as low as 0.084 mg g(-1). The treatment with an aqueous solution NH4OH 7% w/w with heating at 90 degrees C was the most effective, reducing PEs content to 0.063 mg g(-1) (cake) and 0.066 mg g(-1) (bran), below the critical toxicity threshold of 0.1 mg g(-1). Cytotoxicity tests and nutritional assessments confirmed the safety of the treated samples, suggesting that this affordable technology could be scalable for livestock feeding at the industrial level.
BIOMASS CONVERSION AND BIOREFINERY
(2023)
Article
Biotechnology & Applied Microbiology
Elias Alves da Silva, Thais Demarchi Mendes, Thalyta Fraga Pacheco, Raquel Bombarda Campanha, Daiana Wischral, Simone Mendonca, Marli Camassola, Felix Goncalves de Siqueira, Manoel Teixeira Souza Junior
Summary: The study focused on deconstructing oil palm empty fruit bunches (EFBs) using hydrothermal and biological pretreatments. The yields of cellulose, hemicellulose, lignin, extractives, and ashes were measured after autohydrolysis of EFBs. Biological pretreatment with basidiomycetes strains was performed, followed by enzymatic hydrolysis and comparison with commercial enzymes. The results showed that autohydrolysis pretreatment resulted in the best sugar yields, while biological pretreatment provided enzymes and other advantages. Combinations of enzymatic extracts from basidiomycetes and ascomycetes reduced phenolic substances.
BIOFUELS BIOPRODUCTS & BIOREFINING-BIOFPR
(2022)
Article
Biology
Vandinelma Oliveira Vieira, Aparecido Almeida Conceicao, Joice Raisa Barbosa Cunha, Antony Enis Virginio Machado, Euziclei Gonzaga de Almeida, Eustaquio Souza Dias, Lucas Magalhaes Alcantara, Robert Neil Gerard Miller, Felix Goncalves de Siqueira
Summary: This study investigates the use of spent mushroom substrate as a nutrient source for tomato seedlings and the development of an integrated tomato and mushroom co-production system. The results show that the substrate colonized with A. bisporus improves the growth and yield of tomato seedlings, and the co-production of mushrooms and tomatoes is viable.
SAUDI JOURNAL OF BIOLOGICAL SCIENCES
(2022)
Article
Environmental Sciences
Marina Borges Guimaraes, Felix Goncalves de Siqueira, Raquel Bombarda Campanha, Jose Antonio de Aquino Ribeiro, Perola Oliveira Magalhaes, Simone Mendonca
Summary: This study evaluated the growth capacity of seven basidiomycetes in different biomass substrates and their effects on protein content and antioxidant activity. Results showed that several basidiomycetes were able to grow in the substrates and had detoxification and protein enhancement capabilities. These findings suggest the potential use of basidiomycetes in detoxifying JCS and CSC biomasses for animal feed applications.
WASTE AND BIOMASS VALORIZATION
(2022)
Article
Energy & Fuels
Elias Alves da Silva, Thais Demarchi Mendes, Thalyta Fraga Pacheco, Daiana Wischral, Dulce Cristine dos Santos, Simone Mendonca, Marli Camassola, Felix Goncalves de Siqueira, Manoel Teixeira Souza Junior
Summary: The use of residual lignocellulosic biomass offers the opportunity to obtain high-value products like enzymes. This study found that basidiomycetes from the Brazilian cerrado have enormous biotechnological potential to produce enzymatic cocktails from empty fruit bunches and sludge decanter waste from the oil palm industry. The results showed that different combinations of basidiomycetes extracts and commercial cellulases or ascomycetes extracts effectively hydrolyzed pretreated empty fruit bunches, yielding glucose and xylose.
ENERGY SCIENCE & ENGINEERING
(2022)
Article
Chemistry, Applied
Caio de Oliveira Gorgulho Silva, Joice Raisa Barbosa Cunha, Aparecido Almeida Conceicao, Euziclei Gonzaga Almeida, Diego Cunha Zied, Wagner Goncalves Vieira Junior, Eustaquio Souza Dias, Omoanghe S. Isikhuemhen, Patricia Verardi Abdelnur, Felix Goncalves de Siqueira
Summary: The study investigated the effects of indoor and outdoor cultivation on the metabolite profiles of five commercial strains of A. subrufescens using untargeted metabolomics. It was found that the concentration of several metabolites varied significantly between strains and cultivation systems in a strain-dependent manner. Increasing production scale through indoor cultivation may decrease the nutraceutical quality of some strains while affecting taste and browning susceptibility to different extents.
Review
Energy & Fuels
Ruben Dario Romero Pelaez, Marcos Ene Chaves Oliveira, Robert Neil Gerard Miller, Joao Ricardo Moreira de Almeida, Felix Goncalves de Siqueira
Summary: This review summarizes the advances in using lignocellulosic residues from the oil palm industry to obtain various value-added products and highlights the potential of these residues.
BIOMASS CONVERSION AND BIOREFINERY
(2022)
Article
Microbiology
Joice Raisa Barbosa Cunha, Daiana Wischral, Ruben Dario Romero Pelaez, Maria Aparecida de Jesus, Ceci Sales-Campos, Raquel Bombarda Campanha, Thais Demarchi Mendes, Simone Mendonca, Eustaquio Souza Dias, Felix Goncalves de Siqueira
Summary: This study focused on obtaining fermented oil cake (cotton or Jatropha) with potential characteristics for animal feed formulations through macrofungi growth. The experiment showed that fermentation with certain macrofungi can reduce toxic compounds and increase protein concentrations in the products. Fermented products from Coriolopsis sp. and Tyromyces sp. could be important biological inputs for animal feed.
Article
Chemistry, Analytical
Tallyta Santos Teixeira, Jorge Candido Rodrigues Neto, Elias Alves Silva, Aparecido Almeida Conceicao, Felix Goncalves de Siqueira, Patricia Verardi Abdelnur
Summary: Fungi can produce bioactive metabolites, and Mass Spectrometry Imaging (MSI) can provide complementary information about their spatial localization. However, adaptations are needed for MSI in microorganisms, particularly in sample preparation. The imprinting method shows potential for microbial MALDI-MSI. This study compared the classic and imprinting methods on fungi metabolites and identified specific ions related to intermicrobial communication.
BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY
(2023)
Article
Biotechnology & Applied Microbiology
Taisa Godoy Gomes, Samed Ibrahim Isa Abdel Hadi, Jose Antonio de Aquino Ribeiro, Rosana Segatto, Thais Demarchi Mendes, Cristiane Vieira Helm, Aloisio Freitas Chagas Junior, Robert Neil Gerard Miller, Simone Mendonca, Felix Goncalves de Siqueira
Summary: This study screened multiple fungal strains for the degradation of toxic phorbol esters (PE) in Jatropha curcas cake (JCC). Among the evaluated basidiomycetes, Pleurotus pulmonarius displayed the highest efficiency in PE degradation, reaching 97%. Further cultivation of P. pulmonarius in JCC formulations supplemented with lignocellulosic sources resulted in successful fruiting body formation and increased degradation of the toxic compound. The residue of JCC was found to be suitable for animal feed and as a source of enzymes.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2022)
Article
Microbiology
Carlos Godinho de Abreu, Luiz Fernando Wurdig Roesch, Fernando Dini Andreote, Saura Rodrigues Silva, Tatiana Silveira Junqueira de Moraes, Diego Cunha Zied, Felix Goncalves de Siqueira, Eustaquio Souza Dias, Alessandro M. Varani, Victor Satler Pylro
Summary: Agaricus subrufescens, also known as the sun mushroom, has significant nutritional and medicinal value. The genome sequence of A. subrufescens provides valuable information for cultivation alternatives and genetic editing strategies to delay the browning process. The study also identified important genes and provided resources for further fungal biological and genomic studies.
RESEARCH IN MICROBIOLOGY
(2023)
Article
Multidisciplinary Sciences
Taisa Godoy Gomes, Fernando Campos de Assis Fonseca, Gabriel Sergio Costa Alves, Felix Goncalves de Siqueira, Robert Neil Gerard Miller
Summary: The stability of potential reference genes in Pleurotus pulmonarius was evaluated on toxic, non-toxic jatropha cake, and a combined treatment, and PHOS, EF1a, and MNP3 were identified as appropriate reference genes. These genes will facilitate the elucidation of detoxification mechanisms in P. pulmonarius and the analysis of target genes for biorefinery applications.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Joice Raisa Barbosa Cunha, Daiana Wischral, Ruben Dario Romero Pelaez, Perola De Oliveira Magalhaes, Marina Borges Guimaraes, Maria Aparecida de Jesus, Ceci Sales-Campos, Thais Demarchi Mendes, Eustaquio Souza Dias, Simone Mendonca, Felix Goncalves de Siqueira
Summary: This study aimed to investigate the antibacterial activities of aqueous extracts from oil seed cakes fermented by macrofungi and their potential applications in probiotic bacteria culture. The results showed that the fermented extracts promoted the growth of probiotic bacteria and exhibited possible antimicrobial activity.
Article
Biotechnology & Applied Microbiology
Ruben Dario Romero Pelaez, Luana Assis Serra, Daiana Wischral, Joice Raisa Barbosa Cunha, Thais Demarchi Mendes, Thalyta Fraga Pacheco, Felix Goncalves de Siqueira, Joao Ricardo Moreira de Almeida
Summary: This study investigates the co-culture of the fungi Panus lecomtei and the yeast Sporidiobolus pararoseus for laccase production. Results show that the co-culture significantly enhances laccase production and is suitable for the hydrolysis of lignocellulosic biomass and the degradation of unwanted dyes released in the environment.
FERMENTATION-BASEL
(2023)
Article
Biotechnology & Applied Microbiology
Ruben Dario Romero Pelaez, Daiana Wischral, Joice Raisa Barbosa Cunha, Thais Demarchi Mendes, Thalyta Fraga Pacheco, Felix Goncalves de Siqueira, Joao Ricardo Moreira de Almeida
Summary: This study aimed to produce enzymatic extracts from co-cultured fungi with hydrolytic and oxidative activities for efficient hydrolysis of lignocellulosic biomasses. The co-cultures of Trichoderma reesei and Panus lecomtei showed positive interaction and responded positively to variations in temperature and inoculum interval. The enzymatic extract production of co-cultures was improved by modifying the media composition, resulting in higher hydrolysis yields and ethanol production. This work demonstrates the use of fungi co-cultures to produce enzymatic extracts for efficient hydrolysis and fermentation of pretreated lignocellulosic biomass.
FERMENTATION-BASEL
(2022)