Article
Chemistry, Medicinal
Christian Borgo, Luca Cesaro, Tsuyoshi Hirota, Keiko Kuwata, Claudio D'Amore, Thomas Ruppert, Renata Blatnik, Mauro Salvi, Lorenzo A. Pinna
Summary: CK2, a protein kinase with pleiotropic functions, is implicated in global human pathologies, particularly cancer. Selective inhibitors like CX4945 and GO289 show promise in controlling the CK2-dependent phosphoproteome.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Kelsie J. Anson, Giulia A. Corbet, Amy E. Palmer
Summary: In this study, the researchers investigated how changes in intracellular zinc ion levels affect kinase signaling pathways using fluorescent biosensors and cell perturbations. The results showed that zinc ion fluctuations are not toxic and do not activate stress-dependent kinase signaling. Additionally, the study demonstrated that while zinc ions can inhibit phosphatases, ERK and Akt are primarily activated through upstream signaling pathways.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Biochemistry & Molecular Biology
John E. Chojnowski, Rongrong Li, Tiffany Tsang, Fatimah H. Alfaran, Alexej Dick, Simon Cocklin, Donita C. Brady, Todd I. Strochlic
Summary: Casein kinase 2 (CK2) is an evolutionarily conserved enzyme involved in cellular functions, and its dysregulation is linked to diseases like cancer. This study demonstrates that copper enhances CK2 activity by binding directly to the enzyme. Specific residues in the catalytic subunit are critical for copper-binding. Changes in cellular copper levels also affect CK2 activity.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hsin-Yu Ho, Ping-Ju Chen, Yi-Ching Chuang, Yu-Sheng Lo, Chia-Chieh Lin, Ming-Ju Hsieh, Mu-Kuan Chen
Summary: Picrasidine I exerts anticancer effects in nasopharyngeal carcinoma by modulating HO-1 via the ERK and Akt signaling pathways.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Plant Sciences
Lingyan Jiang, Shizi Zhang, Jianbin Su, Scott C. Peck, Lijuan Luo
Summary: This review summarizes the current knowledge of protein kinase signaling pathways in plant-Colletotrichum interaction and their relation to plant immunity and pathogen virulence. It highlights the potential of combining genomic resources and phosphoproteomics research to understand the key molecular components of plant-Colletotrichum interactions. Understanding these molecular interactions is important for breeding disease-resistant cultivars and developing effective strategies to control anthracnose.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Shutang Tan, Christian Luschnig, Jiri Friml
Summary: The plant hormone auxin is central to plant growth and development, with protein phosphorylation emerging as a crucial post-translational modification type regulating auxin output. This review comprehensively discusses the genetics, biochemistry, and cell biology of kinases and phosphatases involved in auxin action, providing insights into the mechanisms of developmental auxin responses and future directions for integrating protein phosphorylation control into the molecular auxin network.
Article
Cell Biology
Lu Qiu, Qiufang Yang, Wenshan Zhao, Yadi Xing, Peng Li, Xiaowen Zhou, Haoming Ning, Ranran Shi, Shanshan Gou, Yalan Chen, Wenjie Zhai, Yahong Wu, Guodong Li, Zhenzhen Chen, Yonggang Ren, Yanfeng Gao, Yiguo Zhang, Yuanming Qi
Summary: The antioxidant transcription factor NFE2L1 regulates glucose and lipid metabolism and acts as an energy sensor. Loss of NFE2L1 leads to impaired glucose uptake and metabolic diseases. Glycosylation enables NFE2L1 to sense energy state and regulate glucose homeostasis. Disruption of NFE2L1 reprograms glucose metabolism, aggravating the Warburg effect and causing mitochondrial damage.
CELL DEATH & DISEASE
(2022)
Article
Biochemistry & Molecular Biology
Yonghwan Shin, Sungmin Kim, Gangning Liang, Tobias S. Ulmer, Woojin An
Summary: VprBP is highly expressed in melanoma cells and phosphorylates threonine 120 (T120) on histone H2A to drive the transcriptional inactivation of growth-regulatory genes. Inhibition of VprBP or its kinase activity could mitigate melanoma tumor growth, indicating the therapeutic potential of targeting VprBP for melanoma treatment.
Review
Biochemistry & Molecular Biology
Anna G. Mankovich, Brian C. Freeman
Summary: Heat shock protein 90 (Hsp90), a highly conserved molecular chaperone, not only maintains the stability of metastable proteins, but also plays a crucial role in protein transport. The specific contributions of Hsp90 to protein transport are still not well defined, despite numerous connections with factors involved in this process.
Review
Pharmacology & Pharmacy
Kha Wai Hon, Syafiq Asnawi Zainal Abidin, Iekhsan Othman, Rakesh Naidu
Summary: Colorectal cancer is characterized by metabolic reprogramming, which plays a crucial role in promoting tumor progression by modulating core metabolic pathways. Activation of oncogenes and loss of tumor suppressor genes regulate metabolic reprogramming, with protein kinases serving as integral components in facilitating interactions between signaling pathways and metabolic pathways in CRC.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Andrea Baier, Ryszard Szyszka, Monika Elzbieta Jach
Summary: Protein kinase CK2 is crucial for cell survival and the regulation of apoptosis. It is found that the yeast protein Asf1 inhibits CK2 activity through its poly-acidic sequence, which also influences cell growth.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Yong Cao, Jie Jiang, Xueqin Song, Xiaoyan Wang, Fang Huang, Yan Li, Li Tang, Mingying Li, Zhuang Chen, Feng Chen, Haisu Wan
Summary: This study reveals a transcription-independent function of EN2 in addition to its role as a transcription factor. EN2 expression activates multiple signaling pathways and interacts with various proteins, affecting their phosphorylation status. Inhibiting the NF-κB and AKT signaling pathways activated by EN2 shows therapeutic potential for EN2-induced malignancy.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Xiaojuan Ren, Chao Feng, Yubo Wang, Pu Chen, Simeng Wang, Jianling Wang, Hongxin Cao, Yujun Li, Meiju Ji, Peng Hou
Summary: SLC39A10 is frequently upregulated in gastric adenocarcinomas and is associated with poor patient outcomes. It enhances the proliferation, invasiveness, and tumorigenic potential of gastric cancer cells by increasing zinc ion influx, through activation of the CK2 pathway. Targeting CK2 may be a potential therapeutic strategy for gastric cancer patients with high SLC39A10 expression.
EXPERIMENTAL AND MOLECULAR MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Konrad Chojnacki, Patrycja Winska, Olena Karatsai, Miroslawa Koronkiewicz, Malgorzata Milner-Krawczyk, Monika Wielechowska, Maria Jolanta Redowicz, Maria Bretner, Pawel Borowiecki
Summary: The newly synthesized compounds showed potent cytotoxic activities against breast cancer cells, with methyl group derivatives more efficiently reducing cell viability. Compounds with higher logP values were found to be more effective intracellular inhibitors of CK2 with better proapoptotic properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Integrative & Complementary Medicine
Ke-Qiang Chen, Da-zhi Li, Zhi-bin Chen, Chuan-lin Zhang, Bin-can Wang, Chun-e Wang
Summary: This study investigated the mechanism of Quanzhenyiqitang in treating lipopolysaccharide-induced lung injury and found that the PYK2/p38MAPK/HDAC2/CK2 pathway is an important mechanism underlying the pathogenic progression of COPD. Quanzhenyiqitang may be a therapeutic drug for preventing chronic inflammation and delaying the progression of COPD by inhibiting PYK2 signaling pathways.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Article
Engineering, Environmental
Guang-Hui Chen, Chang-Chun Song, Tao Zhao, Christer Hogstrand, Xiao-Lei Wei, Wu-Hong Lv, Yu-Feng Song, Zhi Luo
Summary: ZnO NPs are widely used and distributed in the environment, yet their toxicities and mechanisms on physiological processes and metabolism remain largely unknown. This study found that dietary ZnO NPs disturbed hepatic Zn metabolism, increased Zn and lipid accumulation, induced oxidative stress, and activated mitophagy. Suppression of mitochondrial oxidative stress attenuated ZnO NP-induced mitophagy and lipotoxicity.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chong-Chao Zhong, Tao Zhao, Christer Hogstrand, Fang Chen, Chang-Chun Song, Zhi Luo
Summary: Oxidative stress and autophagy are involved in copper-induced lipid deposition, and Nrf2 plays a crucial role in this process, regulating lipid metabolism and lipogenesis. Additionally, oxidative stress promotes Nrf2 recruitment to the PPAR gamma promoter, leading to target gene transcription and subsequent lipogenesis.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2022)
Article
Environmental Sciences
Meaghan N. Evans, Simon Waller, Carsten T. Muller, Benoit Goossens, Jeremy A. Smith, Mohd Soffian Abu Bakar, Peter Kille
Summary: Patterns and practices of agricultural expansion pose a threat to global biodiversity. In this study, the impacts of the global palm oil industry on wildlife species were investigated, with a focus on the wild Malay civets in a degraded landscape in Malaysian Borneo. The levels of metals in the civets' hair were measured, and their association with biological and environmental factors was examined. The study found that metal concentrations were influenced by civet age, weight, proximity to a tributary, and access to oxbow lakes.
ENVIRONMENTAL RESEARCH
(2022)
Article
Engineering, Environmental
Tao Zhao, Wu-Hong Lv, Christer Hogstrand, Dian-Guang Zhang, Yi-Chuang Xu, Yi-Huan Xu, Zhi Luo
Summary: Excessive manganese can be toxic to humans and animals, but its effects and mechanisms on vertebrates have been overlooked. This study found that dietary manganese overload led to hepatic lipotoxicity through mitochondrial dysfunction caused by oxidative stress and acetylation of Sod2 mediated by Mtf1/sirt3. These findings provide important insights into the toxicological and environmental evaluation of manganese in aquatic environments.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Yu-Feng Song, Hua Zheng, Zhi Luo, Christer Hogstrand, Zhen-Yu Bai, Xiao-Lei Wei
Summary: This study reveals the mechanism of HFD-induced mitochondrial dysfunction and its role in hepatic lipid dysregulation through SIRT3/mtHSP70-mediated UPRmt. The choline-activated alleviating effect is also demonstrated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Fisheries
Scott MacAulay, Amy R. Ellison, Peter Kille, Joanne Cable
Summary: Early and accurate diagnosis is crucial in mitigating the impact of infectious diseases in aquatic environments, but it can be challenging due to hidden biodiversity and physical constraints. Traditional diagnostics like visual diagnosis and histopathology are still widely used, but newer technologies such as portable next generation sequencing and artificial intelligence are being tested for early diagnosis. Future methods like AI image diagnosis and drone surveillance have the potential to reduce labor costs without compromising sensitivity, but they require investment in capital and infrastructure.
REVIEWS IN AQUACULTURE
(2022)
Article
Genetics & Heredity
Ilze Rasnaca, Peter Kille, Lindsay K. Newbold, David J. Spurgeon
Summary: This study investigates the effects of long-term exposure to arsenic, cadmium, and fluoranthene on the survival, growth, development, and DNA methylation status of the earthworm Lumbricus rubellus. The results show significant effects on the measured endpoints for all three chemicals. Arsenic and cadmium have no effect on DNA methylation patterns, while fluoranthene influences DNA methylation levels.
Article
Environmental Sciences
Henry Wilde, William Bernard Perry, Owen Jones, Peter Kille, Andrew Weightman, Davey L. Jones, Gareth Cross, Isabelle Durance
Summary: This paper presents a novel method for obtaining real-time estimates of wastewater dilution and total daily volume through wastewater treatment works. The method, which uses several physico-chemical markers in the wastewater, proves effective in recovering capped peaks in wastewater flow measurements and normalizing SARS-CoV-2 viral loads in wastewater samples.
Review
Biochemistry & Molecular Biology
Kathryn M. Taylor
Summary: This review explores the origin of the LIV-1 family of zinc transporters, focusing on the discovery of these nine human proteins. It examines the structural and functional differences between these nine human LIV-1 family members and the five other ZIP transporters. The dependence of the LIV-1 family on various post-translational modifications and their implications are discussed, along with their involvement in cancer. Potential areas for future fruitful discoveries are also suggested.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Food Science & Technology
Irene Cattaneo, Alexander D. D. Kalian, Matteo R. R. Di Nicola, Bruno Dujardin, Sara Levorato, Luc Mohimont, Alexis V. V. Nathanail, Edoardo Carnessechi, Maria Chiara Astuto, Jose V. V. Tarazona, George E. N. Kass, Antoine K. Djien Liem, Tobin Robinson, Paola Manini, Christer Hogstrand, Paul S. S. Price, Jean Lou C. M. Dorne
Summary: The assessment of combined exposure, toxicity, and risk is the main focus of human health and animal health risk assessment when dealing with multiple chemicals. The European Food Safety Authority (EFSA) has recently published guidance documents on both the methodologies and criteria for grouping chemicals. These documents provide principles and methodologies for all steps of risk assessment as well as criteria for grouping chemicals, aiming to improve the accuracy and efficiency of combined risk assessment.
Article
Cell Biology
Zhen-Yu Bai, Hua Zheng, Zhi Luo, Christer Hogstrand, Ling-Jiao Wang, Yu-Feng Song
Summary: This study demonstrated that high-fat diet impairs chylomicrons assembly through unfolded protein response, while choline can alleviate this impairment by regulating the expression level of perk.
Article
Physics, Applied
Iestyn Pope, Nuno G. C. Ferreira, Peter Kille, Wolfgang Langbein, Paola Borri
Summary: The ability to detect small metallic nanoparticles by optical microscopy inside environmentally relevant species may have a wide impact for ecotoxicology studies. We demonstrate four-wave mixing microscopy on individual small gold nanoparticles inside the hepatopancreas of Oniscus Asellus, a terrestrial isopod, which ingests metals found in the soil. Four-wave mixing microscopy holds great potential to investigate the fate of metal nanoparticles inside biological systems.
APPLIED PHYSICS LETTERS
(2023)
Article
Zoology
Rafaela T. Dudas, Wilian C. Demetrio, Liliane S. Maia, Jose N. O. Satiro, Karlo A. Silva, Vitoria B. Nicola, Peter Kille, Cintia M. R. Oliveira, Rita O. Afonso, Georgina Russell, Nuno G. C. Ferreira, Luis Cunha, George G. Brown, Marie L. C. Bartz
Summary: The study assessed earthworm communities in long-term no-tillage sites and nearby secondary Atlantic Forest fragments in Parana state, Brazil. It found 11 earthworm species, with 7 being native species and 4 being exotic or cosmopolitan species. The overall earthworm biomass was higher in the forest sites.
Article
Environmental Sciences
Alexander D. Kalian, Emilio Benfenati, Olivia J. Osborne, David Gott, Claire Potter, Jean-Lou C. M. Dorne, Miao Guo, Christer Hogstrand
Summary: Dimensionality reduction techniques were compared and applied to a mutagenicity dataset to enable deep learning driven QSAR models. Linear techniques such as PCA were sufficient, but non-linear techniques like kernel PCA and autoencoders performed comparably, especially for non-linearly separable datasets. Chemical space analysis revealed that most testing data fell within the applicability domain, with certain regions causing performance issues. Certain dimensionality reduction techniques facilitated unique and beneficial navigations of the chemical space.
Article
Medicine, Research & Experimental
Huw J. Morgan, Elise Rees, Simone Lanfredini, Kate A. Powell, Jasmine Gore, Alex Gibbs, Charlotte Lovatt, Gemma E. Davies, Carlotta Olivero, Boris Y. Shorning, Giusy Tornillo, Alex Tonks, Richard Darley, Eddie C. Y. Wang, Girish K. Patel
Summary: This study found that CD200 protein in basal cell carcinoma may lead to the near absence of NK cells in the tumor microenvironment through ectodomain shedding. CD200 suppresses MAPK signaling in NK cells, blocking indirect and direct cell killing. Blocking CD200 inhibition can restore NK cell killing ability.
JOURNAL OF CLINICAL INVESTIGATION
(2022)