Article
Microbiology
Qiao Hu, Lun Yao, Xia Liao, Liang-Sheng Zhang, Hao-Tian Li, Ting-Ting Li, Qing-Gen Jiang, Mei-Fang Tan, Lu Li, Roger R. Draheim, Qi Huang, Rui Zhou
Summary: A comparative study on STK/STP in Streptococcus suis revealed that STK plays a more critical role in cell division, energy metabolism, and protein translation, while STP primarily impacts bacterial growth. Deletion of stk or stp had significant effects on protein expression, with more virulence factors downregulated in increment stk strains. Overall, both STP and STK are essential regulatory proteins for S. suis, with STK playing a more prominent role in growth, cell division, and pathogenesis.
Article
Cell Biology
Kellie A. Lemoine, Julianna M. Fassas, Shirag H. Ohannesian, Nicole H. Purcell
Summary: PHLPP is a family of enzymes consisting of two isoforms (PHLPP1 and PHLPP2) that modulate intracellular activity by dephosphorylating specific residues on proteins like Akt. Dysregulation of PHLPP has been linked to various diseases, so targeting specific isoforms of PHLPP could be beneficial in treating cardiovascular diseases.
CELLULAR SIGNALLING
(2021)
Article
Biology
Holly Fowle, Ziran Zhao, Qifang Xu, Jason S. Wasserman, Xinru Wang, Mary Adeyemi, Felicity Feiser, Alison N. Kurimchak, Diba Atar, Brennan C. McEwan, Arminja N. Kettenbach, Rebecca Page, Wolfgang Peti, Roland L. Dunbrack, Xavier Grana
Summary: The study elucidates the mechanism by which PP2A/B55 alpha recognizes substrates and dephosphorylates them, identifying a key binding site in p107 and validating the necessity of related SLiM motif sequences for substrate binding.
Article
Chemistry, Medicinal
Elva Morretta, Adama Sidibe, Andrea Spallarossa, Antonello Petrella, Elda Meta, Olga Bruno, Maria Chiara Monti, Chiara Brullo
Summary: In this study, new benzyl-urea derivatives were synthesized, among which 4e showed potent inhibition of HUVEC proliferation. The potential target of 4e in HUVEC was identified as PP1 gamma through interactome analysis, suggesting an interesting anti-angiogenic action at the intracellular level.
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Weixia Gao, Xiaoliang Zhang, Guangtong Zhang, Meng Zuo, Wei Cao, Zhoujie Xie, Hao Liu
Summary: This study identified 25 putative transcriptional repressors in S. zooepidemicus and revealed the regulatory mechanism of ScrR in controlling the production of hyaluronic acid (HA). A deficiency in ScrR resulted in decreased HA production, while overexpression of scrB led to a significant improvement in HA production. These findings contribute to a better understanding of the physiological regulation processes in S. zooepidemicus and could aid in the development of high-performance industrial strains for efficient HA production.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Maru Jaime-Garza, Carlos A. Nowotny, Daniel Coutandin, Feng Wang, Mariano Tabios, David A. Agard
Summary: PP5 requires Hsp90 to dephosphorylate CRaf kinase and Cdc37. Hsp90 acts as a platform to facilitate targeted dephosphorylation by PP5 and collaborates with phosphorylated Cdc37 for the folding and activation of client kinases. The cryo-EM structure reveals how Hsp90 activates and scaffolds PP5 to dephosphorylate nearby phosphorylation sites of CRaf, showing the role of Hsp90 in post-translational modification of client kinases goes beyond folding and activation.
NATURE COMMUNICATIONS
(2023)
Review
Parasitology
Jenny Nancy Gomez-Sandoval, Alma Reyna Escalona-Montano, Abril Navarrete-Mena, M. Magdalena Aguirre-Garcia
Summary: Protein phosphatases in parasites exhibit diverse functions and characteristics, some of which could serve as potential targets for therapy or diagnostic markers. Understanding their involvement in parasite cell cycle, metabolism, virulence, and immune response evasion is crucial for medical and veterinary purposes.
PARASITOLOGY RESEARCH
(2021)
Review
Cell Biology
Pieter Vaneynde, Iris Verbinnen, Veerle Janssens
Summary: Reversible protein phosphorylation is a fundamental regulatory mechanism in eukaryotic cell and organismal physiology. Mutations in serine/threonine protein phosphatases (PSP) have been found to be associated with congenital diseases, most of which are characterized by brain-specific abnormalities. Understanding the pathogenic mechanisms of these diseases and identifying downstream targets and effectors could potentially lead to new therapeutic approaches.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Ezequiel J. Alba-Posse, Carlos David Bruque, Yamila Gandola, Javier Gasulla, Alejandro D. Nadra
Summary: In this study, mutant variants of the PP1 protein were designed and evaluated to enhance their capabilities as a biosensor for detecting microcystins. The successful enhancement of PP1 inhibition suggests the potential of these variants for practical microcystin detection applications.
JOURNAL OF STRUCTURAL BIOLOGY
(2023)
Review
Immunology
Jie-Jie Xu, Hai-Di Li, Xiao-Sa Du, Juan-Juan Li, Xiao-Ming Meng, Cheng Huang, Jun Li
Summary: PSTPIP2, a member of the F-BAR domain family, plays important roles in immune and inflammatory processes, making it a potential therapeutic target for AIDs. While it has been found that proteins like PTP-PEST, SHIP1, and CSK can bind to PSTPIP2 and inhibit AIDs development, the exact mechanisms of PSTPIP2's function remain to be fully elucidated.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Microbiology
Aline Freville, Benedicte Gnangnon, Asma S. Khelifa, Mathieu Gissot, Jamal Khalife, Christine Pierrot
Summary: This review presents an overview of the diversity of phosphatases in Apicomplexan parasites and discusses their various functions throughout the parasites' life cycles. It also explores how this diversity can be utilized for the design of innovative and specific drugs/therapeutic strategies.
Article
Chemistry, Multidisciplinary
Jiajia Yang, Xuewei Xia, Meixia Guo, Li Zhong, Xiaoyong Zhang, Xuewu Duan, Jun Liu, Riming Huang
Summary: Fungi Fusarium proliferatum and its toxins are harmful to agriculture, animals, and human health. This study discovered that naturally derived 2-methoxy-1,4-naphthoquinone (MNQ) has potential inhibitory effects on F. proliferatum and explored the molecular mechanisms involved.
Article
Nutrition & Dietetics
Yasuyo Urasaki, Thuc T. Le
Summary: This study found that cinnamaldehyde and curcumin can increase the phosphorylation of Akt2 at multiple sites, thereby promoting insulin signaling pathway. Inhibiting the activities of PP2A and PTP1B, cinnamaldehyde and curcumin enhance the phosphorylation of Akt2 at critical sites and improve Akt2's response to insulin. These findings provide a theoretical basis for the development of new drugs for insulin resistance syndrome.
Article
Biochemistry & Molecular Biology
Monika J. Stankiewicz, Jian Du, Dominick Martinico, Steven J. Ackerman
Summary: C/EBP epsilon(27) acts as a potent repressor of MBP1 transcription in eosinophil differentiation by interacting with GATA-1 protein and binding to a C/EBP site in the MBP1 promoter.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Mirabela Hali, Brian E. Wadzinski, Anjaneyulu Kowluru
Summary: This study investigated the role of Alpha4, a non-canonical subunit of protein phosphatase 2A, in the regulation of glucose-induced acute and chronic effects in pancreatic beta cells. Alpha4 expression was increased in response to high glucose and C2-Ceramide. Knockdown of Alpha4 attenuated ER stress and cell death induced by high glucose, while preventing the decrease in Connexin36 expression. These findings suggest that Alpha4 contributes to metabolic dysfunction and cell death in pancreatic beta cells under high glucose conditions.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Article
Chemistry, Medicinal
Xue Han, Bin Peng, Bei-Bei Xiao, Sheng-Li Cao, Chao-Rui Yang, Wen-Zhu Wang, Fu-Cheng Wang, Hong-Yun Li, Xiao-Li Yuan, Ruifeng Shi, Ji Liao, Hailong Wang, Jing Li, Xingzhi Xu
EUROPEAN JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Review
Genetics & Heredity
Zheng Li, Xingzhi Xu
Article
Biochemistry & Molecular Biology
Yongtai Bai, Weibin Wang, Siyu Li, Jun Zhan, Hanxiao Li, Meimei Zhao, Xiao Albert Zhou, Shiwei Li, Xiaoman Li, Yanfei Huo, Qinjian Shen, Mei Zhou, Hongquan Zhang, Jianyuan Luo, Patrick Sung, Wei-Guo Zhu, Xingzhi Xu, Jiadong Wang
Review
Genetics & Heredity
Nadana Sabapathi, Shanthi Sabarimurugan, Madhav Madurantakam Royam, Chellan Kumarasamy, Xingzhi Xu, Gaixia Xu, Rama Jayaraj
MOLECULAR DIAGNOSIS & THERAPY
(2019)
Article
Cell Biology
Shanshan Nai, Yingxin Shi, Huanwei Ru, Yuehe Ding, Qizhi Geng, Zhe Li, Meng-Qiu Dong, Xingzhi Xu, Jing Li
Article
Microbiology
Qianting Yang, Mingfeng Liao, Wenfei Wang, Mingxia Zhang, Qi Chen, Jiubiao Guo, Bin Peng, Jian Huang, Haiying Liu, Ayano Yahagi, Xingzhi Xu, Katsuhiko Ishihara, Andrea Cooper, Xinchun Chen, Yi Cai
Article
Biochemistry & Molecular Biology
Fu-Cheng Wang, Bin Peng, Sheng-Li Cao, Hong-Yun Li, Xiao-Li Yuan, Ting-Ting Zhang, Ruifeng Shi, Zhuqing Li, Ji Liao, Hailong Wang, Jing Li, Xingzhi Xu
BIOORGANIC CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Xiaopeng Lu, Ming Tang, Qian Zhu, Qiaoyan Yang, Zhiming Li, Yantao Bao, Ge Liu, Tianyun Hou, Yafei Lv, Ying Zhao, Haiying Wang, Yang Yang, Zhongyi Cheng, He Wen, Baohua Liu, Xingzhi Xu, Luo Gu, Wei-Guo Zhu
NUCLEIC ACIDS RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Xiaolong Tang, Guo Li, Fengting Su, Yanlin Cai, Lei Shi, Yuan Meng, Zuojun Liu, Jie Sun, Ming Wang, Minxian Qian, Zimei Wang, Xingzhi Xu, Yong-Xian Cheng, Wei-Guo Zhu, Baohua Liu
NUCLEIC ACIDS RESEARCH
(2020)
Article
Biology
Fanbiao Meng, Minxian Qian, Bin Peng, Linyuan Peng, Xiaohui Wang, Kang Zheng, Zuojun Liu, Xiaolong Tang, Shuju Zhang, Shimin Sun, Xinyue Cao, Qiuxiang Pang, Bosheng Zhao, Wenbin Ma, Zhou Songyang, Bo Xu, Wei-Guo Zhu, Xingzhi Xu, Baohua Liu
Article
Biochemistry & Molecular Biology
Bin Peng, Ruifeng Shi, Jing Bian, Yuwei Li, Peipei Wang, Hailong Wang, Ji Liao, Wei-Guo Zhu, Xingzhi Xu
Summary: PLK1 is enriched at DSBs in response to DNA damage, but dispersed by PARG. PAR chains directly bind to PLK1 and inhibit its enzymatic activity, while CHK1-mediated phosphorylation affects PLK1 activity towards RAD51, ultimately promoting HR repair.
NUCLEIC ACIDS RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Xuefei Zhu, Xiang-Yang Zheng, Peng Gong, Xingzhi Xu
Summary: This article discusses the role of CLASPIN ubiquitination in the regulation of the ATR-CHK1 signaling pathway and its importance for genome integrity and cell survival. Specifically, the degradation and deubiquitination of CLASPIN play a crucial role in the activation and inactivation of the signaling pathway. Research in this area is progressing rapidly and provides promising opportunities for chemotherapeutic targets.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2022)
Article
Biochemistry & Molecular Biology
Shuailin Hao, Ya Wang, Yuqin Zhao, Wen Gao, Wei Cui, Youhang Li, Jian Cui, Yu Liu, Lixiu Lin, Xingzhi Xu, Hailong Wang
Summary: Protein reversible post-translational modification (PTM) plays a crucial role in cellular processes. Lysine crotonylation (Kcr), a newly identified PTM, is found to be involved in replication stress response. The modification of histone H2A at lysine 119 (H2AK119) by crotonylation and ubiquitination specifically regulates transcription-replication conflicts (TRCs), protecting genome stability under replication stress.
NUCLEIC ACIDS RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Donghui Xia, Xuefei Zhu, Ying Wang, Peng Gong, Hong-Shu Su, Xingzhi Xu
Summary: DNA replication forks are tightly regulated to ensure accurate duplication of genetic information. Replication stress responses stabilize stalled replication forks and repair pathways further contribute to fork stabilization. The signaling network, regulated by post-translational modifications such as ubiquitination, plays a key role in the regulation of replication fork progression and stability. Targeting replication fork stability and the ubiquitination system may have therapeutic implications for cancer treatment.
BIOSCIENCE REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Yuqin Zhao, Kaiping Hou, Youhang Li, Shuailin Hao, Yu Liu, Yinan Na, Chao Li, Jian Cui, Xingzhi Xu, Xiaohua Wu, Hailong Wang
Summary: HELQ has opposing activities in regulating DNA end resection at double strand breaks (DSBs) and stalled forks, and plays a crucial role in maintaining genome stability.
NUCLEIC ACIDS RESEARCH
(2023)