Article
Biochemistry & Molecular Biology
Ke Gu, Guangmang Liu, Caimei Wu, Gang Jia, Hua Zhao, Xiaoling Chen, Gang Tian, Jingyi Cai, Ruinan Zhang, Jing Wang
Summary: This study demonstrated that tryptophan enhances cell migration in porcine intestine epithelial cells (IPEC-J2) through the CaSR/Rac1/PLC-γ1 signaling pathway.
Article
Biochemistry & Molecular Biology
Eun-Ah Cho, Pingfeng Zhang, Vikas Kumar, Mikhail Kavalchuk, Hao Zhang, Qingqiu Huang, James S. Duncan, Jinhua Wu
Summary: This study revealed that RIAM promotes integrin activation by phosphorylation-based interaction with membrane PIP2, which is facilitated by Src kinase-mediated phosphorylation of specific tyrosine residues in the RIAM PH domain.
Article
Cell Biology
Mariana V. Rosemblatt, Brian Parra-Tello, Pedro Briceno, Elizabeth Rivas-Yanez, Suat Tucer, Juan Saavedra-Almarza, Pilar Hormann, Brandon A. Martinez, Alvaro Lladser, Mario Rosemblatt, Caglar Cekic, Maria Rosa Bono, Daniela Sauma
Summary: This study assessed the impact of CD73 deficiency on CD8+ T cells and found that CD73 has a dual effect, favoring homeostatic survival but reducing survival under antigenic stimulation. Special attention should be given to these aspects when considering CD73 blockade in the design of novel antitumor therapies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Pathology
WonKyung Cho, Sharad K. Mittal, Elsayed Elbasiony, Sunil K. Chauhan
Summary: The study found that mast cells exhibit an asymmetrical distribution on the murine ocular surface, with more cells located on the nasal side compared to the temporal side. Damage to the nasal side resulted in more extensive angiogenesis than damage to the temporal side. Blocking mast cells with cromolyn attenuated the asymmetrical neovascularization, suggesting that mast cell distribution significantly influences angiogenic response.
AMERICAN JOURNAL OF PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Matthias Kaestle, Camilla Merten, Roland Hartig, Carlos Plaza-Sirvent, Ingo Schmitz, Ursula Bommhardt, Burkhart Schraven, Luca Simeoni
Summary: Lck plays a crucial role in TCR signaling and has been found to have a novel function downstream, regulating thymocyte differentiation and selection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Valerie Janelle, Mathieu Neault, Marie-Eve Lebel, Dave Maurice De Sousa, Salix Boulet, Ludovic Durrieu, Cedric Carli, Chloe Muzac, Sebastien Lemieux, Nathalie Labrecque, Heather J. J. Melichar, Frederick A. Mallette, Jean-Sebastien Delisle
Summary: The development of T-cell dysfunction upon repeated antigen exposure involves the accumulation of DNA damage and activation of the p38MAPK signaling pathway, leading to cellular senescence features including p16(INK4a) upregulation. However, targeting p16(INK4a) can improve T-cell functionality in exhausted CAR T cells, suggesting T-cell senescence as a potential target in immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biology
Grace L. Edmunds, Carissa C. W. Wong, Rachel Ambler, Emily J. Milodowski, Hanin Alamir, Stephen J. Cross, Gabriella Galea, Christoph Wulfing, David J. Morgan
Summary: This study investigates the role of adenosine receptor A(2A)R and the checkpoint molecule TIM3 in suppressing the killing of Renca tumors by CL4 T cells. The authors find that TIM3, when upregulated on tumor-infiltrating CD8(+) T cells after inhibition of A(2A)R, impairs the cytoskeletal polarization of the CL4 T cells. This suggests that blocking A(2A)R and TIM3 could enhance anti-tumor immune responses.
COMMUNICATIONS BIOLOGY
(2022)
Article
Immunology
Feng Huang, Lei Hu, Yuanmin Zhang, Xingmin Qu, Junji Xu
Summary: The study indicates that BMP4 may regulate the activation and IFN-gamma production of CD4+ T cells through metabolism moderation, suggesting it could be a potential therapeutic supplement in autoinflammatory diseases.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Nikhil Ponnoor Anto, Awadhesh Kumar Arya, Amitha Muraleedharan, Jakeer Shaik, Pulak Ranjan Nath, Etta Livneh, Zuoming Sun, Alex Braiman, Noah Isakov
Summary: The ZAP70 protein tyrosine kinase is essential for T cell activation and differentiation. This study reveals that CypA regulates the activity of ZAP70 in T cells and this regulation can be inhibited by CsA. The findings suggest that CypA plays a role in T cell activation by modulating the activity of downstream effector molecules.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Cell Biology
Qianchong Gu, Xiufeng Zhao, Jie Guo, Qiuzhu Jin, Ting Wang, Wei Xu, Liping Li, Jianhua Zhang, Wei Zhang, Sheng Hong, Fuping Zhang, Baidong Hou, Xuyu Zhou
Summary: Foxp3 is the key transcription factor for regulatory T cells (Tregs). Alternative splicing of Foxp3 in humans leads to the expression of two isoforms with different functions. This study shows that the N-terminal domain of Foxp3 inhibits DNA binding by moving towards the C terminus, facilitated by exon 2. The presence of Foxp3A2 in thymus-derived Tregs (tTregs) reduces their sensitivity to T cell receptor (TCR) stimulation and interleukin-2 (IL-2) responsiveness, while in peripherally induced Tregs (pTregs), Foxp3A2 expression enhances the expression of RORyt-related genes, providing a competitive advantage. Overall, alternative splicing of exon 2 plays a differential role in regulating tTreg and pTreg homeostasis.
Article
Immunology
Guangmang Liu, Ke Gu, Fang Wang, Gang Jia, Hua Zhao, Xiaoling Chen, Caimei Wu, Ruinan Zhang, Gang Tian, Jingyi Cai, Jiayong Tang, Jing Wang
Summary: Tryptophan can enhance intestinal epithelial barrier integrity and decrease inflammatory response through the CaSR/Rac1/PLC-γ1 signaling pathway.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Yilin Feng, Jiaqi Fan, Yifan Cheng, Qionghai Dai, Shaohua Ma
Summary: This study investigates the impact of stress on Alzheimer's disease (AD) using male APP/PS1 transgenic mice. The results show that stress worsens AD pathology, while environmental enrichment (EE) slows down disease progression. Interleukin-6 (IL-6) and IL-10 are identified as key stress-related AD regulators. Furthermore, the CD8+/CD4+ T cell ratio is found to be influenced by stress exposure and EE. Depletion and enrichment of CD8+ T cells both accelerate AD, suggesting a negative impact of immune intervention. These findings highlight the potential of stress management and balanced immunity as novel approaches for AD therapy.
Article
Biochemistry & Molecular Biology
Monika Lakk, Grace F. Hoffmann, Aruna Gorusupudi, Eric Enyong, Amy Lin, Paul S. Bernstein, Trine Toft-Bertelsen, Nanna MacAulay, Michael H. Elliott, David Krizaj
Summary: The study found that membrane cholesterol regulates mechanotransduction in human trabecular meshwork cells, with TRPV4 channels mainly located outside cholesterol-enriched membrane domains. Additionally, the biomechanical environment shapes the lipid content of TM membranes, which can affect intraocular pressure and eye diseases.
JOURNAL OF LIPID RESEARCH
(2021)
Article
Cell Biology
Ju A. Shim, So Min Lee, Jin Woo Jeong, Hyori Kim, Woo Jae Son, Jun Hong Park, Parkyong Song, Sin-Hyeog Im, Sangsu Bae, Jung-Hyun Park, Yuna Jo, Changwan Hong
Summary: This study investigates the molecular mechanisms underlying the regulation of gamma c expression in T cells under T cell receptor stimulation. It identifies NFAT1 and NF kappa B as key regulators in the transcriptional upregulation of gamma c expression.
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Engineering, Biomedical
Yongtao Wang, Yingjun Yang, Xinlong Wang, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang, Guoping Chen
Summary: Cell chirality plays a significant role in controlling gene transfection, with increasing cell adhesion area enhancing efficiency while left-handed and right-handed swirling patterns showing similar levels of gene transfection. Additionally, the swirling of the cell cytoskeleton influences gene transfection efficiency.
Article
Biochemistry & Molecular Biology
Raquel Reyes, Alicia Monjas, Maria Yanez-Mo, Beatriz Cardenes, Giulia Morlino, Alvaro Gilsanz, Yesenia Machado-Pineda, Esther Lafuente, Peter Monk, Francisco Sanchez-Madrid, Carlos Cabanas
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2015)
Review
Biochemistry & Molecular Biology
Nikolaos Patsoukis, Kankana Bardhan, Jessica D. Weaver, Duygu Sari, Alvaro Torres-Gomez, Lequn Li, Laura Strauss, Esther M. Lafuente, Vassiliki A. Boussiotis
Review
Biochemistry & Molecular Biology
Nikolaos Patsoukis, Kankana Bardhan, Jessica D. Weaver, Duygu Sari, Alvaro Torres-Gomez, Lequn Li, Laura Strauss, Esther M. Lafuente, Vassiliki A. Boussiotis
Article
Immunology
Julio Alonso-Padilla, Esther M. Lafuente, Pedro A. Reche
JOURNAL OF IMMUNOLOGY RESEARCH
(2017)
Article
Immunology
Magdalena Molero-Abraham, Jose L. Sanchez-Trincado, Marta Gomez-Perosanz, Alvaro Torres-Gomez, Jose Luis Subiza, Esther M. Lafuente, Pedro A. Reche
FRONTIERS IN IMMUNOLOGY
(2019)
Article
Biochemical Research Methods
Monica J. Quinzo, Esther M. Lafuente, Pilar Zuluaga, Darren R. Flower, Pedro A. Reche
BMC BIOINFORMATICS
(2019)
Review
Immunology
Alvaro Torres-Gomez, Carlos Cabanas, Esther M. Lafuente
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Cell Biology
Alvaro Torres-Gomez, Jose Luis Sanchez-Trincado, Victor Toribio, Raul Torres-Ruiz, Sandra Rodriguez-Perales, Maria Yanez-Mo, Pedro A. Reche, Carlos Cabanas, Esther M. Lafuente
Editorial Material
Immunology
Esther M. Lafuente, Florence Niedergang, Carlos Rosales
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Marta Gomez-Perosanz, Jose L. Sanchez-Trincado, Miguel Fernandez-Arquero, John Sidney, Alessandro Sette, Esther M. Lafuente, Pedro A. Reche
Summary: This study identified and characterized 23 conserved HRV-specific CD8 T cell epitopes on PBMCs from 14 HLA I typed subjects, confirming peptide-specific IFN gamma production and binding to the relevant HLA I for nine of these epitopes. The study also validated cytotoxicity mediated by A*02:01-restricted epitopes and discovered an unusually long 16-mer epitope peptide restricted by A*02:01. These HRV-specific CD8 T cell epitopes are expected to elicit CD8 T cell responses in up to 87% of the population and could be crucial for developing an HRV vaccine.
Article
Biochemistry & Molecular Biology
Beatriz Cardenes, Irene Clares, Victor Toribio, Lucia Pascual, Soraya Lopez-Martin, Alvaro Torres-Gomez, Ricardo Sainz de la Cuesta, Esther M. Lafuente, Manuel Lopez-Cabrera, Maria Yanez-Mo, Carlos Cabanas
Summary: Research has shown that the interaction between integrin alpha 5 beta 1 on CRC cells and their ligand ADAM17 mediates the binding and uptake of CRC-derived exosomes, while the expression of CD9 on exosomes negatively regulates this process.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Marta Gomez-Perosanz, Tara Fiyouzi, Miguel Fernandez-Arquero, John Sidney, Alessandro Sette, Ellis L. Reinherz, Esther M. Lafuente, Pedro A. Reche
Summary: This study identified 7 CD4 T cell epitopes from RV A and C serotypes, demonstrating their ability to bind to multiple HLA II molecules and be recognized by over 95% of the global population when combined. These epitopes could be crucial for monitoring RV infections and developing peptide-based vaccines against most RV A and C serotypes.
Review
Biochemistry & Molecular Biology
Hector F. Pelaez-Prestel, Jose L. Sanchez-Trincado, Esther M. Lafuente, Pedro A. Reche
Summary: The oral mucosa is a highly active site of immune activity where various immune cells play a crucial role in defending against pathogens. It is exposed to a variety of antigens from food and commensal bacteria that need to be tolerated, with mechanisms involving dendritic cells, regulatory T cells, and epithelial cells contributing to this tolerance. These tolerogenic mechanisms in the oral mucosa are intricately intertwined.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Alvaro Ras-Carmona, Hector F. Pelaez-Prestel, Esther M. Lafuente, Pedro A. Reche
Summary: BCEPS is a web server for predicting linear B cell epitopes, tailored to select epitopes that are immunogenic and capable of inducing cross-reactive antibodies with native antigens. The server implements various machine learning models and can rank predicted epitopes based on properties such as flexibility, accessibility, hydrophilicity, and predicted presentation by MHC II molecules.
Review
Biochemistry & Molecular Biology
Tara Fiyouzi, Hector F. Pelaez-Prestel, Raquel Reyes-Manzanas, Esther M. Lafuente, Pedro A. Reche
Summary: Regulatory T cells (Tregs) play a crucial role in maintaining immune homeostasis and self-tolerance. Defects in Tregs are associated with autoimmune and chronic inflammatory disorders. Enhancing Treg cell function and numbers through medical interventions has become a significant focus in developing treatments for these diseases. This article provides an overview of Treg cell ontogeny and function, with a particular emphasis on mucosal Tregs. It discusses various approaches, including adoptive Treg cell transfer therapy and the use of microbiota and dietary products, to enhance immunomodulation by Tregs for therapeutic purposes in autoimmune and chronic-inflammatory diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)