Article
Biochemistry & Molecular Biology
Donghoon Ahn, Donghee Ham, Ka Young Chung
Summary: In recent years, deeper insights into the stepwise conformational changes during GPCR-G protein coupling have been obtained using various techniques such as hydrogen/deuterium exchange mass spectrometry, hydroxyl radical footprinting-mass spectrometry, X-ray crystallography, cryoelectron microscopy, and molecular dynamics simulation.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Jong-Chan Park, Alex Luebbers, Maria Dao, Ana Semeano, Anh Minh Nguyen, Maria P. Papakonstantinou, Stefan Broselid, Hideaki Yano, Kirill A. Martemyanov, Mikel Garcia-Marcos
Summary: In this study, researchers uncover a new mechanism of G protein regulation involving the protein GINIP, which biases inhibitory GPCR responses towards Gbg signaling and dampens Gai-GTP signaling. This mechanism is crucial for maintaining balanced neurotransmission and preventing increased seizure susceptibility in mice.
Article
Biochemistry & Molecular Biology
Maozhen Luo, Zhiwei Han, Guoye Huang, Rongfang Li, Yi Liu, Junjie Lu, Lin Liu, Rui Miao
Summary: In this review, the structural characteristics of guanine nucleotide-binding (G) proteins and their binding modes with GTP or ATP were compared and analyzed, providing new insights into the enormous diversity of biological function of G proteins.
PLANT SIGNALING & BEHAVIOR
(2022)
Review
Plant Sciences
R. Ofoe
Summary: Heterotrimeric G-proteins regulate important signaling pathways for growth and development in both plants and animals. Understanding the complexities of plant G-proteins could potentially impact global food security by improving crop development. Further research on molecular mechanisms underlying G-protein signaling in diverse plant species will be essential for enhancing our understanding and improving crop improvement.
Article
Biochemical Research Methods
Brian D. Young, Jihui Sha, Ajay A. Vashisht, James A. Wohlschlegel
Summary: This study identified novel regulators of G beta gamma signaling in human cells using mass spectrometry, with KCTD2 and KCTD5 shown to specifically recognize G beta gamma and be involved in downstream signal transduction. These KCTD proteins act as substrate adaptors for a multisubunit CUL3-RING ubiquitin ligase, promoting monoubiquitination of G beta(1/2) lysine-23 and regulating downstream signaling. Depletion of these adaptors impairs downstream signaling in cancer cell lines.
JOURNAL OF PROTEOME RESEARCH
(2021)
Review
Plant Sciences
Ruchi Tiwari, Naveen C. Bisht
Summary: The review summarizes the advanced understanding of heterotrimeric G-protein research in model plants and their emerging roles in modulating plant architecture and agronomical traits in crop species. The discovery of plant-specific G-protein components and unique modes of G-protein cycle regulation in plants highlight the differences between plant and animal G-protein signaling. While extensive studies in Arabidopsis have confirmed the involvement of G-proteins in plant growth, development, and stress adaptation, research in crop species is still in its infancy. However, a few studies suggest important roles of G-proteins in regulating plant architecture and key agronomical traits, indicating their potential in managing plant yield and fitness in changing environments.
Article
Plant Sciences
Akshaya K. Biswal, Ting-Ying Wu, Daisuke Urano, Remi Pelissier, Jean-Benoit Morel, Alan M. Jones, Ajaya K. Biswal
Summary: In this study, the non-canonical XLG proteins were found to play important roles in regulating rice plant growth, grain filling, panicle phenotype, stress tolerance, and disease resistance. The results suggest that genetic manipulation of XLGs has the potential to improve agronomic properties in rice.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Jana Haase, Aimee K. C. Jones, Conor J. Mc Veigh, Eric Brown, Gerard Clarke, Gudrun Ahnert-Hilger
Summary: The study explores the regulation of the serotonin transporter (SERT) by guanine nucleotide-binding protein alpha (G alpha) q in G alpha q knockout mice, revealing sex differences in SERT activity, possibly due to differential expression of G alpha i1 in female mice. The findings suggest a novel mechanism of SERT regulation and highlight the impact of sex differences in diseases associated with dysregulation of serotonin transmission.
JOURNAL OF NEUROCHEMISTRY
(2021)
Article
Chemistry, Analytical
Anna Pepanian, Paul Sommerfeld, Renata Kasprzyk, Toni Kuehl, F. Ayberk Binbay, Christoph Hauser, Reik Loeser, Robert Wodtke, Marcelina Bednarczyk, Mikolaj Chrominski, Joanna Kowalska, Jacek Jemielity, Diana Imhof, Markus Pietsch
Summary: A new method based on fluorescence anisotropy was developed to determine the affinity of ligands at the GTP-binding site and quantify the fraction of active G alpha i1 protein. An advanced bacterial expression protocol was used to produce active human G alpha i1 protein, and its GTP binding capability was determined with novel fluorescently labeled guanine nucleotides.
ANALYTICAL CHEMISTRY
(2022)
Article
Plant Sciences
Parinita Majumdar, Maria Daniela Torres Rodriguez, Sona Pandey
Summary: As sessile organisms, plants are exposed to various environmental stresses that harm their growth and development, leading to significant crop yield reductions worldwide. To cope with adverse conditions, plants have developed adaptive mechanisms, in which the heterotrimeric G-protein complex plays a crucial role. This complex regulates diverse cellular signaling pathways and is involved in plant stress responses, making it a key target for generating stress-tolerant crops.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Review
Biochemistry & Molecular Biology
Natsumi Maruta, Yuri Trusov, Alan M. Jones, Jose R. Botella
Summary: Plant G proteins consist of canonical G alpha subunits and unconventional XLG subunits, interacting with G beta gamma dimers and RGS protein, and participating in development, stress responses, and immunity. Despite the overall similarity between plant and animal G alpha subunits, they convey signals differently.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Developmental Biology
Timothy E. Gookin, Sarah M. Assmann
Summary: This study reports a previously unidentified macroscopic Arabidopsis organ called cantil, which is regulated by the receptor-like kinase ERECTA and heterotrimeric G proteins. Cantil development is strongest at the first nodes after the transition from vegetative to reproductive growth.
Article
Plant Sciences
Boominathan Mohanasundaram, Audrey Dodds, Vandna Kukshal, Joseph M. Jez, Sona Pandey
Summary: Heterotrimeric G-proteins show sporadic presence in algae and specific components are frequently lost in land plants. The signaling pathways mediated by these proteins influence growth, development, and physiology in plants. The existence of XLGαβγ trimers is conserved in the entire land plant lineage, but their presence is sporadic in algae. The different components have distinct evolutionary histories.
Article
Chemistry, Multidisciplinary
Corinne A. Lutomski, Tarick J. El-Baba, Joshua D. Hinkle, Idlir Liko, Jack L. Bennett, Neha V. Kalmankar, Andrew Dolan, Carla Kirschbaum, Kim Greis, Leonhard H. Urner, Parth Kapoor, Hsin-Yung Yen, Kevin Pagel, Christopher Mullen, John E. P. Syka, Carol V. Robinson
Summary: By using an infrared laser in a high-pressure linear ion trap, researchers were able to remove detergent micelles and obtain good sequence coverage for membrane proteins and their complexes through top-down mass spectrometry.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Yiping Wang, Hailei Zhang, Pengxi Wang, Huan Zhong, Wuzhen Liu, Shoudong Zhang, Liming Xiong, Yingying Wu, Yiji Xia
Summary: Pattern-triggered immunity (PTI) is a vital defense mechanism used by plants against pathogen attacks. Heterotrimeric G proteins, particularly XLG2 and XLG3, play a positive role in regulating plant immunity, while XLG1 was previously believed to be non-functional. However, this study shows that all three XLGs function redundantly in PAMP-triggered MAPK activation and plant immunity. The triple knockout mutants of xlg1 xlg2 xlg3 exhibited compromised resistance against pathogen infection.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2023)