Article
Microbiology
Om Basukala, Oscar Trejo-Cerro, Michael P. Myers, David Pim, Paola Massimi, Miranda Thomas, Corrado Guarnaccia, David Owen, Lawrence Banks
Summary: HPV-E7 plays a crucial role in HPV-induced malignancy by targeting AP2 complex and modulating clathrin-mediated endocytosis, which may contribute to cellular transformation.
Article
Multidisciplinary Sciences
Nathan R. Zaccai, Zuzana Kadlecova, Veronica Kane Dickson, Kseniya Korobchevskaya, Jan Kamenicky, Oleksiy Kovtun, Perunthottathu K. Umasankar, Antoni G. Wrobel, Jonathan G. G. Kaufman, Sally R. Gray, Kun Qu, Philip R. Evans, Marco Fritzsche, Filip Sroubek, Stefan Hoening, John A. G. Briggs, Bernard T. Kelly, David J. Owen, Linton M. Traub
Summary: Clathrin-mediated endocytosis (CME) is a key mechanism for controlling the cell surface proteome in mammalian cells. This study shows that FCHO plays a crucial role in this process by marking the initiation sites of clathrin-coated pits (CCPs) and interacting with other proteins to drive the growth and maturation of CCPs.
Article
Biochemistry & Molecular Biology
G. F. Senguel, R. Mishra, E. Candiello, P. Schu
Summary: AP2 forms AP2 CCV with clathrin and other coat proteins, and synapses contain different types of CCV. The stability and composition of CCV are regulated by various factors, including Hsc70 and phosphorylation patterns. The knockout of the AP1/O1B complex disrupts synaptic vesicle recycling and endosomal protein sorting, leading to upregulation of endocytosis. Stable CCV, termed stCCV, have distinct characteristics and specialized functions in synaptic plasticity. The phosphorylation of Hsc70 and the levels of kinases play a crucial role in regulating the stability and disassembly of clathrin in CCV.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
(2024)
Article
Multidisciplinary Sciences
Valentina Corradi, Anna N. Bukiya, Williams E. Miranda, Meng Cui, Leigh D. Plant, Diomedes E. Logothetis, D. Peter Tieleman, Sergei Y. Noskov, Avia Rosenhouse-Dantsker
Summary: This study reveals the molecular mechanism of cholesterol's effect on ion channel activity. Through mutagenesis and simulation experiments on the Kir3.4 channel, researchers identified a molecular switch that controls the regulation of the channel by cholesterol. This switch changes the conformation of the channel, leading to different effects of cholesterol on the channel's activity.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Hui Jing Lim, Jacinta M. Wubben, Cristian Pinero Garcia, Sebastian Cruz-Gomez, Jieru Deng, Jeffrey Y. W. Mak, Abderrahman Hachani, Regan J. Anderson, Gavin F. Painter, Jesse Goyette, Shanika L. Amarasinghe, Matthew E. Ritchie, Antoine Roquilly, David P. Fairlie, Katharina Gaus, Jamie Rossjohn, Jose A. Villadangos, Hamish E. G. McWilliam
Summary: MR1 is a conserved microbial immune-detection system in mammals that presents antigens to specific lymphocytes, contributing to host defense and tissue repair. This study reveals that human MR1 interacts with the endocytic adaptor protein 2 (AP2) complex to regulate its internalization from the cell surface, thus controlling antigen presentation and microbial metabolic detection.
JOURNAL OF CELL BIOLOGY
(2022)
Editorial Material
Neurosciences
Giorgia Pedini, Claudia Bagni
Summary: Mutations in epigenetic factors are linked to autism spectrum disorder (ASD). In this Neuron paper, Yan et al. (2022) demonstrate that the antagonism between ASH1L and PRC2 alters the balance of histone methylation at the ephrin receptor A7 locus, resulting in decreased expression of EphA7, synaptic pruning deficits, and ASD-like behaviors.
Article
Developmental Biology
Lisa Redlingshofer, Frances M. Brodsky
Summary: The AP2 adaptor complex plays a central role in regulating clathrin-mediated endocytosis, serving as a key hub for protein interactions beyond cargo recognition and clathrin recruitment. It mediates clathrin coated pit maturation and couples lattice formation to membrane deformation. AP2 complements the attenuating role of clathrin light chain subunits in driving clathrin assembly and dynamic lattice rearrangement for budding.
CELLS & DEVELOPMENT
(2021)
Article
Multidisciplinary Sciences
A. R. Gomes, A. Marin-Menendez, S. H. Adjalley, C. Bardy, C. Cassan, M. C. S. Lee, A. M. Talman
Summary: Sexual reproduction and meiotic sex are fundamental in eukaryotes, but the mechanisms determining sex or mating types are diverse. This study reveals the essential role of the md1 gene in determining male fate in the human malaria parasite. The research uncovers a non-genetic mechanism of sex determination and highlights Md1 as a potential target for malaria transmission interventions.
Article
Biochemistry & Molecular Biology
Ioana Stejerean-Todoran, Katharina Zimmermann, Christine S. Gibhardt, Adina Vultur, Christian Ickes, Batool Shannan, Zurine Bonilla del Rio, Anna Wolling, Sabrina Cappello, Hsu-Min Sung, Magdalena Shumanska, Xin Zhang, Maithily Nanadikar, Muhammad U. Latif, Anna Wittek, Felix Lange, Andrea Waters, Patricia Brafford, Jorg Wilting, Henning Urlaub, Dorthe M. Katschinski, Peter Rehling, Christof Lenz, Stefan Jakobs, Volker Ellenrieder, Alexander Roesch, Michael P. Schon, Meenhard Herlyn, Hedwig Stanisz, Ivan Bogeski
Summary: MCUA expression correlates with melanoma patient survival and MCUA knockdown suppresses melanoma cell growth and stimulates migration and invasion. Knockdown of MCUA reduces tumor volumes but promotes lung metastases.
Article
Biochemistry & Molecular Biology
Vishnu Balaji, Leonie Mueller, Robin Lorenz, Eva Kevei, William H. Zhang, Ulises Santiago, Jan Gebauer, Ernesto Llamas, David Vilchez, Carlos J. Camacho, Wojciech Pokrzywa, Thorsten Hoppe
Summary: The ubiquitin ligase CHIP plays a crucial role in substrate selection and processing through its dimer-monomer transition and autoubiquitylation mechanisms, allowing it to respond and regulate cellular stress.
Article
Microbiology
Christina Manner, Raphael Dias Teixeira, Dibya Saha, Andreas Kaczmarczyk, Raphaela Zemp, Fabian Wyss, Tina Jaeger, Benoit-Joseph Laventie, Sebastien Boyer, Jacob G. Malone, Katrine Qvortrup, Jens Bo Andersen, Michael Givskov, Tim Tolker-Nielsen, Sebastian Hiller, Knut Drescher, Urs Jenal
Summary: HecRE is a molecular switch that regulates cyclic di-GMP levels and promotes binary cell states during Pseudomonas aeruginosa surface colonization and biofilm development. This finding provides a new approach to control surface infections.
NATURE MICROBIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Sangpil Kim, Batakrishna Jana, Eun Min Go, Ji Eun Lee, Seongeon Jin, Eun-Koung An, Juyoung Hwang, Youjung Sim, Sehee Son, Dohyun Kim, Chaekyu Kim, Jun-O Jin, Sang Kyu Kwak, Ja-Hyoung Ryu
Summary: The study developed an intramitochondrial polymerization-induced self-assembly system for regulating the fate of cancer cells, inducing cell necroptosis by increasing ROS levels. This in situ polymerization system shows great potential for cancer treatment, including drug-resistant cancers.
Article
Genetics & Heredity
Abhishek Kumar Mishra, Cornelia Fritsch, Roumen Voutev, Richard S. Mann, Simon G. Sprecher
Summary: Visual perception is mediated by specialized photoreceptor neurons of the eye, each expressing photosensitive opsins sensitive to specific wavelengths of light. The study has shown that Homothorax controls a binary Rhodopsin switch in the ocelli of fruit flies to regulate the expression of Rhodopsin 2 and 1. The presence of conserved Hth binding sites in the promoter region of rhodopsin 1 and rhodopsin 2 between different Drosophila species suggests a critical role of Hth in evolution.
Article
Chemistry, Multidisciplinary
Xing-Yu Liu, Wei Xiong, Qian-Qian Qi, Hui-Min Ji, Yu-Tong Zhang, Hua-Jun Lei, Jian Liu, Ping Yin, Tian Tian, Xiang Zhou
Summary: Chemical methods have the potential to regulate CRISPR activity for spatiotemporal control of gene editing. We have developed a small-molecule strategy to switch off CRISPR functionality and successfully terminated CRISPR-Cas9-mediated genome editing in human cells.
CELL REPORTS PHYSICAL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Yang Chen, Shaoqin Hu, Xuanang Wu, Zhenli Xie, Yuan Wang, Bianbian Wang, Xiaopeng Li, Yingmei Pei, Yuhao Gu, Kai Huang, Jingxiao Huo, Anqi Wei, Cheng Bi, Zhe Lu, Qian Song, Huadong Xu, Xinjiang Kang, Shuli Shao, Jiangang Long, Jiankang Liu, Zhuan Zhou, Rong Huang, Zuying Chai, Changhe Wang
Summary: Exocytosis and endocytosis are tightly coupled processes. The primary Ca2+ sensor Syt1 plays bidirectional roles in this coupling by promoting small-sized endocytosis but inhibiting large-sized bulk endocytosis. Ca2+ binding ability is crucial for Syt1 to regulate both types of endocytic pathways.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Correction
Cell Biology
Antoni G. Wrobel, Zuzana Kadlecova, Jan Kamenicky, Ji-Chun Yang, Torsten Herrmann, Bernard T. Kelly, Airlie J. McCoy, Philip R. Evans, Stephen Martin, Stefan Mueller, Susanne Salomon, Filip Sroubek, David Neuhaus, Stefan Honing, David J. Owen
DEVELOPMENTAL CELL
(2020)
Article
Multidisciplinary Sciences
Oleksiy Kovtun, Veronica Kane Dickson, Bernard T. Kelly, David J. Owen, John A. G. Briggs
Article
Multidisciplinary Sciences
Lisa Redlingshofer, Faye McLeod, Yu Chen, Marine D. Camus, Jemima J. Burden, Ernest Palomer, Kit Briant, Philip N. Dannhauser, Patricia C. Salinas, Frances M. Brodsky
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biotechnology & Applied Microbiology
Giulia Gallo, Carina Conceicao, Christina Tsirigoti, Brian Willett, Stephen C. Graham, Dalan Bailey
Summary: By using random mutagenesis and structural modeling, it was found that the binding region between PPRV H and ovine SLAMF1 is functionally conserved across all morbilliviruses. Error-prone PCR is a powerful tool for characterizing functional domains within viral proteins.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Microbiology
Tomasz H. Benedyk, Julia Muenzner, Viv Connor, Yue Han, Katherine Brown, Kaveesha J. Wijesinghe, Yunhui Zhuang, Susanna Colaco, Guido A. Stoll, Owen S. Tutt, Stanislava Svobodova, Dmitri I. Svergun, Neil A. Bryant, Janet E. Deane, Andrew E. Firth, Cy M. Jeffries, Colin M. Crump, Stephen C. Graham
Summary: The HSV-1 protein pUL21 functions as an adaptor for protein phosphatase 1 (PP1), directing the dephosphorylation of cellular and viral proteins. By antagonizing the activity of the virus-encoded kinase pUS3, pUL21 plays a crucial role in balancing kinase and phosphatase activities for optimal virus replication and spread.
Article
Biotechnology & Applied Microbiology
William N. D. Gao, Chen Gao, Janet E. Deane, David C. J. Carpentier, Geoffrey L. Smith, Stephen C. Graham
Summary: Researchers have solved the crystal structure of the vaccinia virus E2 protein, revealing its unique folded domains. Recent advances in deep learning methods have greatly improved the accuracy of predicting protein structures, which has significant implications for structural virology and molecular virology.
JOURNAL OF GENERAL VIROLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chris H. Hill, Georgia M. Cook, Sawsan Napthine, Anuja Kibe, Katherine Brown, Neva Caliskan, Andrew E. Firth, Stephen C. Graham, Ian Brierley
Summary: The 2A protein of TMEV stimulates PRF during infection by recognizing RNA elements and forming pseudoknots. Experiments using disome analysis identified ribosome stacking at the TMEV frameshifting signal.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Multidisciplinary Sciences
Chris H. Hill, Lukas Pekarek, Sawsan Napthine, Anuja Kibe, Andrew E. Firth, Stephen C. Graham, Neva Caliskan, Ian Brierley
Summary: This study investigates the structural and functional characteristics of the 2A protein in cardioviruses, revealing its mechanism for stimulating PRF by stabilizing RNA elements and potentially influencing translation through interactions with ribosomes. The findings provide insights into how RNA recognition by 2A promotes PRF efficiency and how 2A accumulation may impact translation during virus infection.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Nathan R. Zaccai, Zuzana Kadlecova, Veronica Kane Dickson, Kseniya Korobchevskaya, Jan Kamenicky, Oleksiy Kovtun, Perunthottathu K. Umasankar, Antoni G. Wrobel, Jonathan G. G. Kaufman, Sally R. Gray, Kun Qu, Philip R. Evans, Marco Fritzsche, Filip Sroubek, Stefan Hoening, John A. G. Briggs, Bernard T. Kelly, David J. Owen, Linton M. Traub
Summary: Clathrin-mediated endocytosis (CME) is a key mechanism for controlling the cell surface proteome in mammalian cells. This study shows that FCHO plays a crucial role in this process by marking the initiation sites of clathrin-coated pits (CCPs) and interacting with other proteins to drive the growth and maturation of CCPs.
Article
Microbiology
Kamal L. Nahas, Viv Connor, Katharina M. Scherer, Clemens F. Kaminski, Maria Harkiolaki, Colin M. Crump, Stephen C. Graham
Summary: Herpes simplex virus-1 (HSV-1) infection causes morphological changes in cellular compartments, as well as specific organelles. These changes can be captured using soft X-ray tomography, which reveals the significant impact of HSV-1 infection on the morphology of cellular compartments.
Article
Biochemistry & Molecular Biology
Tomasz H. Benedyk, Viv Connor, Eve R. Caroe, Maria Shamin, Dmitri I. Svergun, Janet E. Deane, Cy M. Jeffries, Colin M. Crump, Stephen C. Graham
Summary: Herpes simplex virus-1 (HSV-1) alters cellular membrane lipid composition during infection. This study demonstrates that a virus-encoded protein, pUL21, promotes the conversion of ceramide (Cer) to sphingomyelin (SM) by activating CERT. The study also reveals the importance of specific protein-protein interactions in HSV-1 mediated sphingolipid metabolism.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biology
Iain M. Hay, Katie E. Mulholland, Tiffany Lai, Stephen C. Graham, Hayley J. Sharpe, Janet E. Deane
Summary: The research demonstrates that PTPRK selectively dephosphorylates substrates by binding to Afadin, indicating that PTP substrate specificity can be determined by protein-protein interactions. This phosphorylation-independent interaction, mediated through binding to a non-catalytic domain, highlights the potential function of receptor PTPs as intracellular scaffolds.
Article
Biochemistry & Molecular Biology
Iain M. Hay, Maria Shamin, Eve R. Caroe, Ahmed S. A. Mohanned, Dmitri I. Svergun, Cy M. Jeffries, Stephen C. Graham, Hayley J. Sharpe, Janet E. Deane
Summary: Type IIB receptor protein tyrosine phosphatases mediate cell adhesion and signaling through their extracellular and cytoplasmic domains, respectively. The crystal structure of PTPRK has revealed an intermembrane adhesion mode consistent with other family members. Comparison with PTPRM structure suggests that conformational differences between the domains may contribute to homophilic specificity. Analysis of the full-length PTPRM and PTPRK proteins using small-angle X-ray scattering reveals rigid extended conformations and one residue difference at the interaction interface that affects dimer formation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Virology
Natalia S. S. Barbosa, Juan O. O. Concha, Luis L. P. daSilva, Colin M. M. Crump, Stephen C. C. Graham
Summary: Oropouche virus causes a debilitating illness called Oropouche fever, which is common in South America. The virus has the ability to reassort its genome and has caused multiple epidemics in the region over the last 50 years, posing a significant threat to public health. The study investigates the cellular determinants and mechanisms involved in Oropouche virus replication and secretion.
JOURNAL OF VIROLOGY
(2023)
Correction
Biochemistry & Molecular Biology
M. Lherbette, L. Redlingshoefer, F. M. Brodsky, I. A. T. Schaap, P. N. Dannhauser