Article
Multidisciplinary Sciences
Wei Wei, Daniel J. Gaffney, Patrick F. Chinnery
Summary: Analysis of mtDNA in a large number of iPSC lines reveals that iPSC-specific mutations affect a higher proportion of mtDNA molecules, favoring non-synonymous protein-coding and tRNA variants. Additionally, stable heteroplasmy in mtDNA variants during cell differentiation influences mitochondrial metabolism and cell differentiation.
NATURE COMMUNICATIONS
(2021)
Article
Microbiology
Ning Lu, Samuel W. Duvall, Guohong Zhao, Kimberley A. Kowallis, Chao Zhang, Wei Tan, Jingxian Sun, Haley N. Petitjean, Dylan T. Tomares, Guo-Ping Zhao, W. Seth Childers, Wei Zhao
Summary: The direct interaction between PopZ and PodJ has been revealed, which plays a primary role in triggering the bipolarization of PopZ from monopolar during cell development. Disruption of the PodJ-PopZ interaction impairs PopZ-mediated chromosome segregation and positioning.
Article
Biochemistry & Molecular Biology
Silje Anda, Erik Boye, Kay Oliver Schink, Beata Grallert
Summary: Cellular asymmetry is a key factor in the ageing and evolution of multicellular organisms. Research shows that even in simple unicellular eukaryotes, the ability to distinguish 'old' from 'new' and segregate DNA asymmetrically are inherent features of cells.
Article
Immunology
Johannes Dirks, Oliver Andres, Luisa Paul, Georgi Manukjan, Harald Schulze, Henner Morbach
Summary: The expression of IgD in human naive B cells has a significant impact on their maturation and Ig selection, shaping the composition of naive B cells and being involved in selecting specific Ig V-H segments for pre-immune Ig repertoire. Naive B cells lacking IgD expression showed decreased responsiveness in vitro to signals from IgM-BCR, TLR7/9, or CD40 pathways. Furthermore, the frequency of IgM(lo/-) B cells lacking IgD was significantly reduced in the mature naive B cell compartment.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Oncology
Guillaume Hatte, Claude Prigent, Jean-Pierre Tassan
Summary: Our study examined the formation and activation of the contractile ring in the epithelium of Xenopus embryos and explored the involvement of adherens junctions in this process. We found that proteins related to contractile ring formation and activation accumulate in a polarized manner, starting at the apical cell-cell contacts and spreading towards the basal side within seconds. Additionally, we discovered that adherens junctions play a role in the kinetics of contractile ring formation from the beginning of cytokinesis.
EXPERIMENTAL CELL RESEARCH
(2021)
Review
Cell Biology
Zizhu Li, Ying Yi Zhang, Haomiao Zhang, Jiaxuan Yang, Yongze Chen, Hezhe Lu
Summary: Asymmetric cell division is a fundamental process for generating cell diversity and maintaining stem cell populations. In tumor cells, asymmetric division is closely associated with cancer initiation and progression, leading to intratumoral heterogeneity. Studying the relationship between ACD and cancer may provide new approaches for combating cancer heterogeneity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Yanqun Li, Jianhui Yue, Yuan Liu, Jun Wu, Min Guan, Di Chen, Haobo Pan, Xiaoli Zhao, William W. Lu
Summary: The study revealed that strontium promotes osteogenic differentiation of mesenchymal stem cells and balances stemness maintenance and osteogenic differentiation through asymmetric cell division. Strontium activates noncanonical Wnt signaling to regulate cell division and enhance osteogenic differentiation.
ACTA BIOMATERIALIA
(2021)
Article
Multidisciplinary Sciences
Junfeng Liu, Virginija Cvirkaite-Krupovic, Diana P. Baquero, Yunfeng Yang, Qi Zhang, Yulong Shen, Mart Krupovic
Summary: Archaeal virus STSV2 interferes with the cell cycle of its host Sulfolobus islandicus, leading to giant cell formation and asymmetric division. The CRISPR-Cas system plays a key role in defending against the virus. This study sheds light on how archaeal viruses manipulate host cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Review
Biochemistry & Molecular Biology
Jeroen de Keijzer, Alejandra Freire Rios, Viola Willemsen
Summary: In multicellular organisms, cell proliferation and specialization are crucial for development, and in plants, these processes heavily rely on the spatial organization of cells within tissues. Plant cells maintain their relative positions at the moment of division, and the developmental trajectories of daughter cells are often determined by division plane positioning in the parental cell. The moss Physcomitrium patens serves as a suitable model system for studying division plane control, as it undergoes a transition from a simple to a complex three-dimensional body plan formation, involving specific mechanisms such as polarity protein complexes and cytoskeletal structures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Anthony Goudin, Jean-Luc Ferat, Christophe Possoz, Francois-Xavier Barre, Elisa Galli
Summary: This study investigates the process of how Vibrio cholerae spheroplasts return to growth and reveals some key factors and mechanisms involved in the de novo establishment of polarized cellular organization. The elimination of excess periplasm is driven by peptidoglycan synthase aPBPs, while elongation and branching rely on peptidoglycan synthase PBP2. The cell division protein FtsK specifically targets the sites of vesicle extrusion. Additionally, genetic material is amplified through synchronous waves of DNA replication as periplasmic elimination begins, and the polarity factor HubP is involved in the formation of new cell morphology.
Article
Multidisciplinary Sciences
Dorian Obino, Mathieu Maurin, Florent Dingli, Damarys Loew, Aurianne Lescure, Emmanuel Terriac, Christel Goudot, Odile Malbec, Danielle Lankar, Maria-Isabel Yuseff, Ana-Maria Lennon-Dumenil, Helene D. Moreau
Summary: Cell polarity is a crucial process in cell function and is triggered by external signals that relocate the centrosome, defining the cell's polarity axis. This study used B cells as a model to identify new molecular regulators of cell polarity through a medium-throughput siRNA-based imaging screen. The researchers first conducted a quantitative proteomic analysis to identify candidate proteins associated with the centrosome in resting and stimulated B cells. They then performed an siRNA screen and identified hits that regulate centrosome and/or lysosome polarization in B cells upon stimulation. The dataset generated provides valuable information for scientists studying the molecular mechanisms of cell polarity.
Article
Biology
Fernando Y. Maeda, Jurriaan J. H. van Haaren, David B. Langley, Daniel Christ, Norma W. Andrews, Wenxia Song
Summary: Recognition of antigen by B-cell receptor results in localized plasma membrane permeabilization, triggering PM repair responses involving lysosomal exocytosis to facilitate antigen internalization and presentation. Higher affinity antigens cause more B-cell permeabilization and are efficiently presented to T-cells.
Review
Cell Biology
Sydney Treichel, Marie-Dominique Filippi
Summary: Hematopoietic stem cells have the ability to self-renew or differentiate into various blood cell lineages. The cell fate decisions of self-renewal or differentiation must be tightly controlled to prevent diseases like bone marrow failure or leukemia. Studies have shown that the cell cycle length plays a crucial role in hematopoietic stem cell fate. Cell cycle kinetics, divisional history, asymmetric cell divisions, metabolic and organelle activity all have an impact on hematopoietic stem cell fate decisions.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Immunology
Ying Fu, Luman Wang, Baichao Yu, Damo Xu, Yiwei Chu
Summary: B-cell-mediated humoral immune response is crucial for protection against infectious diseases. The metabolism of B cells plays a significant role in their homeostasis, development, and function. While the impact of immunometabolism on B cells is not as deeply studied as that on T cells, there is growing evidence suggesting that intracellular metabolism influences B cell functions through transcriptional regulation and signal transduction. This review provides an update on the recent developments in B cell immunometabolism, including its role in development, differentiation, and function in metabolic, autoimmune, and inflammatory disorders.
Article
Plant Sciences
M. Arif Ashraf, Le Liu, Michelle R. Facette
Summary: Asymmetric cell division is a characteristic feature of development in multicellular organisms, generating different cell types. The maize stomatal development serves as a plant model system to study this process, particularly the asymmetric division of subsidiary mother cells. In this study, we focused on a mutant of an outer nuclear membrane protein that is part of the LINC complex, which is involved in interphase nuclear envelope localization. Our findings suggest that nuclear envelope proteins play a role in premitotic nuclear migration and stable nuclear positioning, and that the position of the nucleus influences the establishment of the division plane in asymmetrically dividing cells.
Review
Biochemistry & Molecular Biology
Phillip M. Brailey, Marta Lebrusant-Fernandez, Patricia Barral
Article
Multidisciplinary Sciences
Luciano da Silva Selistre, Sandrine Lemoine, Allyriane Dantec, Fanny Buron, Vandrea Carla de Souza, Mariana Bertoldo, Carlos Eduardo Poli-de-Figueiredo, Thomas Rimmele, Olivier Thaunat, Lionel Badet, Emmanuel Morelon, Antoine Sicard, Laurence Dubourg
Review
Food Science & Technology
Maxime Espi, Laetitia Koppe, Denis Fouque, Olivier Thaunat
Article
Urology & Nephrology
Jasper Callemeyn, Evelyne Lerut, Henriette de Loor, Ingrid Arijs, Olivier Thaunat, Alice Koenig, Vannary Meas-Yedid, Jean-Christophe Olivo-Marin, Philip Halloran, Jessica Chang, Lieven Thorrez, Dirk Kuypers, Ben Sprangers, Leentje Van Lommel, Frans Schuit, Marie Essig, Wilfried Gwinner, Dany Anglicheau, Pierre Marquet, Maarten Naesens
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2020)
Article
Urology & Nephrology
Sophie Caillard, Dany Anglicheau, Marie Matignon, Antoine Durrbach, Clarisse Greze, Luc Frimat, Olivier Thaunat, Tristan Legris, Valerie Moal, Pierre Francois Westeel, Nassim Kamar, Philippe Gatault, Renaud Snanoudj, Antoine Sicard, Dominique Bertrand, Charlotte Colosio, Lionel Couzi, Jonathan M. Chemouny, Christophe Masset, Gilles Blancho, Jamal Bamoulid, Agnes Duveau, Nicolas Bouvier, Nathalie Chavarot, Philippe Grimbert, Bruno Moulin, Yannick Le Meur, Marc Hazzan
KIDNEY INTERNATIONAL
(2020)
Article
Urology & Nephrology
Arnaud Del Bello, Olivier Thaunat, Moglie Le Quintrec, Oriol Bestard, Antoine Durrbach, Peggy Perrin, Philippe Gatault, Frederic Jambon, Georges-Philippe Pageaux, Laura Llado, Camille Besch, Louise Barbier, Martine Neau-Cransac, Jerome Dumortier, Nassim Kamar
KIDNEY INTERNATIONAL REPORTS
(2020)
Article
Surgery
Jasper Callemeyn, Heleen Ameye, Evelyne Lerut, Aleksandar Senev, Maarten Coemans, Elisabet Van Loon, Ben Sprangers, Vicky Van Sandt, Maud Rabeyrin, Valerie Dubois, Olivier Thaunat, Dirk Kuypers, Marie-Paule Emonds, Maarten Naesens
Summary: The study evaluated the changes in the Banff classification for antibody-mediated rejection (ABMR) and their impact on transplant outcomes and case numbers, finding an increase in cases from Banff'01 to Banff'13 and variable outcomes for reclassified sABMR in Banff'17. The clinical and histological heterogeneity of ABMR is inadequately represented in a binary classification system.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Surgery
Sophie Caillard, Nathalie Chavarot, Helene Francois, Marie Matignon, Clarisse Greze, Nassim Kamar, Philippe Gatault, Olivier Thaunat, Tristan Legris, Luc Frimat, Pierre F. Westeel, Valentin Goutaudier, Mariam Jdidou, Renaud Snanoudj, Charlotte Colosio, Antoine Sicard, Dominique Bertrand, Christiane Mousson, Jamal Bamoulid, Christophe Masset, Antoine Thierry, Lionel Couzi, Jonathan M. Chemouny, Agnes Duveau, Valerie Moal, Gilles Blancho, Philippe Grimbert, Antoine Durrbach, Bruno Moulin, Dany Anglicheau, Yvon Ruch, Charlotte Kaeuffer, Ilies Benotmane, Morgane Solis, Yannick LeMeur, Marc Hazzan, Francois Danion
Summary: Transplant recipients had a higher COVID-19-related mortality compared to nontransplant hospitalized patients but did not differ significantly in the rate of severe disease. Factors associated with severe COVID-19 included age, cardiovascular disease, dyspnea, fever, lymphopenia, and CRP.
AMERICAN JOURNAL OF TRANSPLANTATION
(2021)
Article
Urology & Nephrology
Alice Koenig, Sarah Mezaache, Jasper Callemeyn, Thomas Barba, Virginie Mathias, Antoine Sicard, Beatrice Charreau, Maud Rabeyrin, Frederique Dijoud, Cecile Picard, Vannary Meas-Yedid, Jean-Christophe Olivo-Marin, Emmanuel Morelon, Maarten Naesens, Valerie Dubois, Olivier Thaunat
Summary: The study found that the presence of missing self may exacerbate DSA-dependent NK cell activation, leading to worsening chronic antibody-mediated rejection. Detection of missing self in allograft endothelial cells can help identify patients at higher risk for kidney transplant failure.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2021)
Letter
Urology & Nephrology
Olivier Thaunat, Camille Legeai, Olivier Bastien, Sophie Caillard
KIDNEY INTERNATIONAL
(2021)
Article
Urology & Nephrology
Lionel Couzi, Miriam Manook, Sophie Caillard, Eric Epailly, Benoit Barrou, Dany Anglicheau, Mathias Buchler, Sacha Mussot, Jerome Dumortier, Olivier Thaunat, Laurent Sebbag, Gilles Blancho, Yannick Le Meur, Yuval A. Patel, Mariya Samoylova, Lisa McElroy, Brian Shaw, Scott Sanoff, Marc Hazzan
Summary: During the Covid-19 pandemic in France, the majority of transplant candidates preferred to undergo transplantation as soon as possible rather than waiting for the pandemic to end. Many patients felt inadequately informed by their transplant centers during the pandemic, highlighting the need for improved communication and services.
NEPHROLOGIE & THERAPEUTIQUE
(2021)
Article
Immunology
Thomas Barba, Emmanuel Bachy, Alizee Maarek, Gaelle Fossard, Laurent Genestier, Dany Anglicheau, Valerie Moal, Jacques Dantal, Philippe Rieu, Jonathan Chemouny, Manon Charrier, Antoine Durrbach, Francois Provot, Didier Ducloux, Pierre-Francois Westeel, Anne-Elisabeth Heng, Jean-Philippe Rerolle, Benoit Barrou, Philippe Grimbert, Valerie Chatelet, Christiane Mousson, Benedicte Janbon, Vincent Pernin, Luc Frimat, Nacera Ouali, Denis Glotz, Antoine Thierry, Christophe Mariat, Mathias Buchler, Philippe Gaulard, Veronique Leblond, Emmanuel Morelon, Valerie Dubois, Gilles Salles, Sophie Caillard, Olivier Thaunat
Summary: T/NK-cell PTLD occurs later after transplantation, has worse overall survival, with two subtypes: cutaneous and systemic, the latter having a poorer prognosis. Compared with T/NK-cell lymphomas in nontransplanted patients, systemic T/NK-cell PTLD has lower overall survival.
Article
Medicine, General & Internal
Mathilde Prezelin-Reydit, Valerie Dubois, Sophie Caillard, Anne Parissiadis, Isabelle Etienne, Francoise Hau, Laetitia Albano, Monique Pourtein, Benoit Barrou, Jean-Luc Taupin, Christophe Mariat, Lena Absi, Cecile Vigneau, Virginie Renac, Gwendaline Guidicelli, Jonathan Visentin, Pierre Merville, Olivier Thaunat, Lionel Couzi
Summary: Prospective serial monitoring of medication non-adherence using the Morisky scale does not predict the occurrence of de novo donor-specific antibodies (dnDSA), rejection, or graft failure in kidney transplant recipients. However, the use of cyclosporine and withdrawal of calcineurin inhibitors (CNI) were strongly associated with the development of dnDSA and rejection, leading to graft failure.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cell Biology
Rachel Ulferts, Elena Marcassa, Lewis Timimi, Liam Changwoo Lee, Andrew Daley, Beatriz Montaner, Suzanne Dawn Turner, Oliver Florey, John Kenneth Baillie, Rupert Beale
Summary: Aside from its common association with autophagosomes, LC3 can be recruited to membranes through covalent lipidation in various non-canonical contexts, such as responses to ionophores. Different factors, including the RALGAP complex for M2-induced LC3 lipidation, ATG4D for LC3 recycling, and a vacuolar ATPase subunit for non-canonical LC3 recruitment, may play roles in lipidation processes in different environments. Influenza-induced and ionophore drug-induced LC3 lipidation can result in the association of vacuolar ATPase and ATG16L1, which can be inhibited by Salmonella SopF, suggesting a potential response to damage by various invasive pathogens.
Article
Virology
Marion Cremoni, Sebastien Cuozzo, Emanuela Martinuzzi, Susana Barbosa, Nadia Ben Hassen, Filippo Massa, Elisa Demonchy, Matthieu Durand, Olivier Thaunat, Vincent Esnault, Moglie Le Quintrec, Sophie Caillard, Nicolas Glaichenhaus, Antoine Sicard
Summary: Kidney transplant recipients with low T cell responsiveness at the beginning of COVID-19 infection are at a higher risk of progressing to severe pneumonia. This study provides valuable insights for clinicians managing immunosuppressive drugs in organ transplant recipients.