Article
Construction & Building Technology
O. Kammouh, M. Nogal, R. Binnekamp, A. R. M. Wolfert
Summary: This article introduces a method and tool for construction project management, aiming to dynamically control the project to ensure timely completion. The method combines nonlinear stochastic optimization techniques and probabilistic Monte Carlo analysis, and the developed decision support tool (MitC) automates and optimizes the search for the best mitigation strategies on the run.
AUTOMATION IN CONSTRUCTION
(2022)
Article
Chemistry, Analytical
Xia Qiu, Qingshan Dai, Haoran Tang, Yongxin Li
Summary: This research developed a method to simultaneously detect miRNA-21 and Let-7a in a single experiment by utilizing the distinguishability of two types of current signals. The immobilization of ssDNA1 complementary to miRNA-21 on Pt nanoparticles and ssDNA2 complementary to Let-7a on Ag nanoparticles inhibited the respective current transients. The addition of miRNA-21 and Let-7a disrupted the weak DNA-nanoparticle interaction and restored the catalytic activities, enabling the quantification of the target miRNAs based on the frequency of the recovered current signals.
ANALYTICAL CHEMISTRY
(2023)
Article
Medicine, Research & Experimental
Alexey M. Petrov, Guzalia F. Zakirjanova, Irina Kovyazina, Andrei N. Tsentsevitsky, Ellya A. Bukharaeva
Summary: This study investigated the influence of nerve activity and adrenoceptor stimulation on exocytosis modes in the motor nerve terminal. It was found that high frequency activity promoted neurotransmitter release via kiss-and-run mechanism, while noradrenaline and alpha 2-adrenoreceptors agonist favored full-collapse exocytosis. This has implications for the regulation of respiratory system during stress.
Article
Chemistry, Physical
Si-Min Lu, Meng-Yin Li, Yi-Tao Long
Summary: Single-entity electrochemistry allows for the measurement of single cells, particles, and molecules at the nanoscale using well-defined interfaces. The characteristics of the nanoconfined electrode interface have a significant impact on the assay limitation and selectivity of single-entity measurement, which is controlled by the dynamic chemistry interactions between the target and the electrode interface.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Physical
Si-Min Lu, Meng-Yin Li, Yi-Tao Long
Summary: This article discusses the use of single-entity electrochemistry to measure single cells, particles, and molecules at the nanoscale, and how nanoconfined electrode interfaces enhance measurement characteristics. Stochastic single-entity collision electrochemistry and nanopore electrochemistry are explored as examples of how interactions affect charge transfer and mass transport.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Multidisciplinary
Bohuai Xiao, Suhang He, Mingjun Sun, Jianghao Zhou, Zhiye Wang, Yunchuan Li, Simin Liu, Werner M. Nau, Shuai Chang
Summary: This study introduces a versatile recognition tunneling technique using doubly cucurbit[7]uril-functionalized electrodes to form supramolecular junctions that capture analytes dynamically by host-guest complexation, resulting in characteristic changes in their single-molecule conductance. For structurally related drug molecules and mixtures, distinct current switching signals related to their intrinsic conductance properties and pH-dependent effects are observed.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Materials Science, Multidisciplinary
Anna N. Morozovska, Sergei V. Kalinin, Mykola E. Yelisieiev, Jonghee Yang, Mahshid Ahmadi, Eugene A. Eliseev, Dean R. Evans
Summary: This research explores the polar states of uniaxial ferroelectric nanoparticles interacting with the surface system of electronic and ionic charges, taking into account the broad distribution of mobilities. The results show that nonlinear interactions between the ferroelectric dipoles and surface charges give rise to a wide range of paraelectric-like, antiferroelectric-like ionic, and ferroelectric-like ferroionic states. The transition between these states can be controlled by the static characteristics and relaxation dynamics of the surface charges. The obtained results are not only promising for advanced applications of ferroelectric nanoparticles in nanoelectronics and optoelectronics but also provide strategies for experimental verification.
Article
Multidisciplinary Sciences
Peiran Zhang, Joseph Rufo, Chuyi Chen, Jianping Xia, Zhenhua Tian, Liying Zhang, Nanjing Hao, Zhanwei Zhong, Yuyang Gu, Krishnendu Chakrabarty, Tony Jun Huang
Summary: This study introduces acoustoelectronic nanotweezers, which combine the precision of electronic tweezers with the large-field dynamic control of acoustic tweezers, demonstrating complex patterning of sub-100nm objects.
NATURE COMMUNICATIONS
(2021)
Article
Multidisciplinary Sciences
Gang Wu, Chen Qian, Wen-Li Lv, Xiaona Zhao, Xian-Wei Liu
Summary: Direct visualization of surface chemical dynamics in solution is challenging but essential for understanding nanocatalysis and electrochemistry. We present an azimuth-modulated plasmonic imaging technique that achieves high spatial resolution and enables comprehensive analyses of surface chemical dynamics. This method eliminates interference from reflected light, resulting in a 67-fold increase in the spatial resolution of plasmonic imaging. The technique is demonstrated using electrochemical redox reactions over single silver nanowires, showcasing its potential for studying interfacial chemical reactions.
NATURE COMMUNICATIONS
(2023)
Article
Optics
Xi Xie, Xianyou Wang, Changjun Min, Haixiang Ma, Yunqi Yuan, Zhangyu Zhou, Yuquan Zhang, Jing Bu, Xiaocong Yuan
Summary: This study proposes a new method for accurately trapping and manipulating single or multiple particles in holographic optical surface-wave tweezers. By adjusting the optical potential wells formed by surface waves, the targeted single particle can be trapped while pushing away surrounding particles, and the particle can be dynamically controlled using a holographic tweezers beam. Different particle samples, including gold particles and biological cells, can be applied in this system. This method has significant potential applications in single-particle spectroscopy, particle sorting, and nano-assembly.
PHOTONICS RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Bo Jiang, Wenjie Gu, Wenxuan Jiang, Mengqi Lv, Ben Niu, Xue-Jun Wu, Wei Wang, Hui Wang
Summary: This study demonstrated a simple optical technique to directly observe the transient interfacial electronic coupling events during the electrochemical oxidation of single Ag nanoparticles on an Au electrode. The results showed that the interfacial electronic coupling facilitates the electron transfer of single nanoparticles and provides important insight into understanding the detailed mechanism of nanoelectrochemistry.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Patrick H. Maag, Florian Feist, Vinh X. Truong, Hendrik Frisch, Peter W. Roesky, Christopher Barner-Kowollik
Summary: We introduce a class of single-chain nanoparticles (SCNPs) that can unfold completely in response to visible light (λ(max)=415 nm). The initial folding is achieved through a simple esterification reaction of the polymer backbone, introducing bimane moieties, which allow for light-driven unfolding by reversing the ester-bond formation.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Physical
Wuwen Yan, Siyu Chen, Pan Li, Ronglu Dong, Hyun-Hang Shin, Liangbao Yang
Summary: In this study, a platform for monitoring label-free molecules at the single-molecule level using surface-enhanced Raman spectroscopy (SERS) was developed based on the photothermal effect of gold nanorods (GNRs). The results showed that single crystal violet (CV) molecules can be observed blinking for up to 4 minutes using dynamic surface-enhanced Raman spectroscopy (D-SERS). The research provides a novel idea for exploring the temporal and spatial dynamic processes of different reactions.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Physical
Wuwen Yan, Siyu Chen, Pan Li, Ronglu Dong, Hyun-Hang Shin, Liangbao Yang
Summary: In this study, a platform for monitoring single molecules using surface-enhanced Raman spectroscopy (SERS) was developed based on the photothermal effect of gold nanorods (GNRs). By utilizing laser re-irradiation to create nanometer gaps between GNRs, the blinking behavior of single crystal violet (CV) molecules was observed for up to 4 minutes using dynamic surface-enhanced Raman spectroscopy (D-SERS). The confinement of CV molecules in sub-nanometer spaces and the dynamic capture of their orientations provide a new approach for exploring the temporal and spatial dynamic processes of different reactions.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2023)
Article
Chemistry, Multidisciplinary
Xin Qi, Yundi Zhao, Kacper Lachowski, Julia Boese, Yifeng Cai, Orion Dollar, Brittney Hellner, Lilo Pozzo, Jim Pfaendtner, Jaehun Chun, Francois Baneyx, Christopher J. Mundy
Summary: This study develops a theoretical framework that combines interactions at the molecular and macroscopic scales based on colloidal theory and atomistic molecular dynamics simulations. It accurately captures the macroscopic behavior of complex bioinspired material systems.
Article
Neurosciences
Sofie Charlotte Sundberg, Sarah Helen Lindstrom, Gonzalo Manuel Sanchez, Bjorn Granseth
JOURNAL OF COMPARATIVE NEUROLOGY
(2018)
Article
Biochemistry & Molecular Biology
Selena G. Burgess, Manjeet Mukherjee, Sarah Sabir, Nimesh Joseph, Cristina Gutierrez-Caballero, Mark W. Richards, Nicolas Huguenin-Dezot, Jason W. Chin, Eileen J. Kennedy, Mark Pfuhl, Stephen J. Royle, Fanni Gergely, Richard Bayliss
Article
Medicine, Research & Experimental
Anna Mathia Klawonn, Michael Fritz, Anna Nilsson, Jordi Bonaventura, Kiseko Shionoya, Elahe Mirrasekhian, Urban Karlsson, Maarit Jaarola, Bjorn Granseth, Anders Blomqvist, Michael Michaelides, David Engblom
JOURNAL OF CLINICAL INVESTIGATION
(2018)
Article
Neurosciences
Sofie C. Sundberg, Bjorn Granseth
NEUROSCIENCE LETTERS
(2018)
Review
Cell Biology
Poonam Shah, Alexandre Chaumet, Stephen J. Royle, Frederic A. Bard
Article
Neurosciences
Sarah H. Lindstrom, Sofie C. Sundberg, Max Larsson, Fredrik K. Andersson, Jonas Broman, Bjorn Granseth
Article
Cell Biology
Gabrielle Larocque, Penelope J. La-Borde, Nicholas Clarke, Nicholas J. Carter, Stephen J. Royle
JOURNAL OF CELL BIOLOGY
(2020)
Article
Cell Biology
Cansu Kuey, Gabrielle Larocque, Nicholas I. Clarke, Stephen J. Royle
JOURNAL OF CELL SCIENCE
(2019)
Article
Cell Biology
Ellis L. Ryan, James Shelford, Teresa Massam-Wu, Richard Bayliss, Stephen J. Royle
Summary: The research identified TACC3 and clathrin as core members, and chTOG and GTSE1 as ancillary members of the complex. Additionally, PIK3C2A was shown not to be a part of the complex. Targeting the TACC3-clathrin interface or their microtubule-binding sites are the two most likely strategies to disrupt spindle stability mediated by this multiprotein complex.
JOURNAL OF CELL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Sarah M. Smith, Gabrielle Larocque, Katherine M. Wood, Kyle L. Morris, Alan M. Roseman, Richard B. Sessions, Stephen J. Royle, Corinne J. Smith
Summary: The interaction between Clathrin and the AP2 complex plays a crucial role in coated-pit assembly, with a beta 2 appendage able to bind in at least two positions in the clathrin cage. This multi-modal binding is shown to be a fundamental property of clathrin-AP2 interactions.
Article
Cell Biology
Gabrielle Larocque, Daniel J. Moore, Meghane Sittewelle, Cansu Kuey, Joseph H. R. Hetmanski, Penelope J. La-Borde, Beverley J. Wilson, Nicholas Clarke, Patrick T. Caswell, Stephen J. Royle
Summary: The study investigates the role of intracellular nanovesicles (INVs) in cell migration, showing that overexpression of TPD52-like family proteins affects cell migration, while depletion inhibits it. The research highlights the importance of proper membrane traffic regulation in cell migration and invasion, with a focus on the role of TPD52-like proteins in controlling the recycling of integrins.
JOURNAL OF CELL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Gabrielle Larocque, Stephen J. Royle
Summary: Intracellular nanovesicles (INVs) are a newly discovered class of small transport vesicles that play vital roles in cellular compartment trafficking, particularly in cell migration and invasion. This review comprehensively describes INVs, including their involvement in transport processes such as integrin recycling, and identifies key questions for future research.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Cell Biology
Nuria Ferrandiz, Laura Downie, Georgina P. Starling, Stephen J. Royle
Summary: Misalignment of chromosomes during mitosis can result in chromosome missegregation and the formation of micronuclei, which are associated with cancer. A study showed that chromosomes located beyond the exclusion zone become ensheathed in multiple layers of endomembranes, leading to delayed mitosis and increased frequency of chromosome missegregation and micronucleus formation. Clearance of endomembranes can rescue those chromosomes that were destined for missegregation, indicating that endomembranes promote the missegregation of misaligned chromosomes outside the exclusion zone and pose a risk factor for aneuploidy.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Biology
Cansu Kuey, Meghane Sittewelle, Gabrielle Larocque, Miguel Hernandez-Gonzalez, Stephen J. Royle
Summary: This study demonstrates that intracellular clathrin-coated vesicle (CCV) formation can be induced using minimal machinery in living cells, and it can occur on various membranes. The study also shows that the formation of these vesicles, called MitoPits, can faithfully recapitulate all stages of CCV formation without the involvement of accessory proteins.
Article
Cell Biology
Me'ghane Sittewelle, Nuria Ferrandiz, Mary Fesenko, Stephen J. Royle
Summary: The biology of a cell is composed of dynamic processes, controlled by various proteins and molecules. Microscopy plays a crucial role in understanding these processes at a molecular level. Genetically encoded imaging tools, categorized into observation, inhibition, and activation strategies, have been developed to study protein function in dynamic processes. This article provides examples and a guide on using these tools to dissect protein function in cellular processes, with a focus on rapid protein modification and observing resulting cell state changes, inspiring readers for future imaging experiments.
JOURNAL OF CELL SCIENCE
(2023)
