Article
Biology
Andreas Gerondopoulos, Philipp Brauer, Tomoaki Sobajima, Zhiyi Wu, Joanne L. Parker, Philip Biggin, Francis A. Barr, Simon Newstead
Summary: The study investigates the selective retrieval of ER proteins by the KDEL receptor, revealing a dynamic process of signal capture as it enters the binding pocket. Additionally, it uncovers the affinity differences between HDEL and KDEL, attributing the discrepancy to interactions between specific residues at the -4 position of the signal and residues in the receptor binding pocket.
Article
Cell Biology
Xiuping Sun, Divyanshu Mahajan, Bing Chen, Zhiwei Song, Lei Lu
Summary: The mechanism of Golgi localization of Golgi glycosyltransferases has been found to be primarily through retention rather than retrieval. The study also revealed the contributions of different regions of ST6GAL1 to Golgi retention, showing that the N-terminal cytosolic tail and transmembrane domain can act as Golgi export signals.
JOURNAL OF CELL SCIENCE
(2021)
Review
Plant Sciences
Olga A. Zabotina, Ning Zang, Richard Weerts
Summary: Glycosyltransferases (GTs) are enzymes that attach activated sugar to acceptor substrates. While progress has been made in cloning genes encoding polysaccharide-synthesizing GTs, the majority of GTs remain uncharacterized. The mechanism by which they synthesize complex polysaccharide structures in the Golgi membrane is largely unknown.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Yu-Shin Sou, Junji Yamaguchi, Hiroshi Kameda, Keisuke Masuda, Yusuke Maeda, Yasuo Uchiyama, Masato Koike
Summary: GPHR is important for maintaining proper cholesterol levels and neuronal morphology by regulating the acidic pH of the Golgi apparatus. Knockout of GPHR leads to abnormal Golgi morphology and neurodegeneration.
Article
Plant Sciences
Liang Zhang, Jingwen Ma, Huan Liu, Qian Yi, Yanan Wang, Jingjing Xing, Peipei Zhang, Shengdong Ji, Mingjun Li, Jingyuan Li, Jinbo Shen, Jinxing Lin
Summary: The R-SNAREs VAMP721 and VAMP722 in Arabidopsis are found to play crucial roles in intracellular trafficking, affecting auxin distribution and plant growth by regulating the polar localization of auxin transporters.
Article
Biochemistry & Molecular Biology
Fernando M. Ruggiero, Natalia Martinez-Koteski, Viviana A. Cavieres, Gonzalo A. Mardones, Gerardo D. Fidelio, Aldo A. Vilcaes, Jose L. Daniotti
Summary: A physical association between ganglioside glycosyltransferases and Golgi phosphoprotein 3 has been discovered, which can regulate the expression pattern of glycolipids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Lana Buzuk, Doris Hellerschmied
Summary: The Golgi apparatus plays a crucial role in the secretory pathway of eukaryotic cells, processing proteins and organizing their transportation to other cellular compartments. It is involved in cell surface formation, cell polarity, cell-cell communication, immune signaling, DNA damage response, and mitosis. Maintaining Golgi integrity and protein homeostasis is essential, as Golgi fragmentation and dysfunction are linked to neurodegenerative diseases and certain cancers. Recent studies have revealed the importance of ubiquitin signaling in maintaining Golgi integrity and protein quality control, similar to the endoplasmic reticulum. Ubiquitination helps prevent the accumulation of toxic protein aggregates and regulates Golgi structural rearrangements in response to cellular stress.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Laura Hobohm, Tomas Koudelka, Fenja H. Bahr, Jule Truberg, Sebastian Kapell, Sarah-Sophie Schacht, Daniel Meisinger, Marion Mengel, Alexander Jochimsen, Anna Hofmann, Lukas Heintz, Andreas Tholey, Matthias Voss
Summary: This study identifies more than 20 substrates of the intramembrane protease SPPL3 and demonstrates its significant role in Golgi enzyme turnover, secretion, and regulation of global glycosylation pathways.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Biology
Charlotte Toustou, Marie-Laure Walet-Balieu, Marie-Christine Kiefer-Meyer, Marine Houdou, Patrice Lerouge, Francois Foulquier, Muriel Bardor
Summary: N-glycosylation is an important post-translational modification that is highly conserved in eukaryotes, bacteria, and archaea. The processing of N-glycans in eukaryotes is sequential and involves specific steps in the secretory pathway. Recent research has also highlighted the regulation of N-glycosylation, including the biosynthesis of complex-type N-glycans and the role of TMEM165 in different eukaryotic clades.
BIOLOGICAL REVIEWS
(2022)
Article
Oncology
Artem N. Pachikov, Ryan R. Gough, Caroline E. Christy, Mary E. Morris, Carol A. Casey, Chad A. LaGrange, Ganapati Bhat, Anatoly Kubyshkin, Iryna I. Fomochkina, Evgeniya Y. Zyablitskaya, Tatiana P. Makalish, Elena P. Golubinskaya, Kateryna A. Davydenko, Sergey N. Eremenko, Jean-Jack M. Riethoven, Amith S. Maroli, Thomas S. Payne, Robert Powers, Alexander Y. Lushnikov, Amanda J. Macke, Armen Petrosyan
Summary: The progression of prostate cancer (PCa) is associated with the translocation of S1P and S2P proteases to the endoplasmic reticulum (ER) and subsequent cleavage of ATF6, leading to enhanced unfolded protein response (UPR) and cell proliferation. By utilizing various ER stress models, it was discovered that PCa cells employ an elegant mechanism to auto-activate ER stress and promote cell proliferation.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2021)
Review
Biochemistry & Molecular Biology
Nava Reznik, Deborah Fass
Summary: The formation of disulfide bonds in proteins involves various enzymes and chaperones in the endoplasmic reticulum (ER) and Golgi apparatus. QSOX1 and QSOX2 are Golgi-resident enzymes that catalyze disulfide bonding. The localization of these catalysts in the Golgi has been puzzling, but recent studies have shown that QSOX1 also regulates glycosyltransferases in the Golgi. Additionally, some disulfide-mediated multimerization events in the Golgi are independent of QSOX1 and instead depend on low pH conditions.
Article
Agronomy
Yunhua Zhang, Chaoya Liu, Ruoheng Jin, Yue Wang, Tingwei Cai, Zhijie Ren, Kangsheng Ma, Shun He, Kwang S. Lee, Byung R. Jin, Jianhong Li, Hu Wan
Summary: The study found that Duox-dependent ROS is involved in regulating UGT expression-mediated insecticide resistance in the brown planthopper. Overexpression of NlUGT386F2 contributed to resistance to clothianidin, while inhibition of ROS decreased UGT expression and increased susceptibility to the insecticide. Silencing Duox increased susceptibility to clothianidin by down-regulating UGT expression.
PEST MANAGEMENT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Lida Wu, Tongri Liu, Yuchun Gu
Summary: Significant cellular morphology changes were observed in renal tubules of diabetes patients and animal models, with unclear interaction between insulin and tubular epithelial cells microvillar structure. Insulin treatment was found to increase microvilli length and density through the PI3K/PLCγ signaling pathway, presenting a novel insulin signaling transduction mechanism for understanding renal tubular epithelial cell microvilli dynamic regulation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Plant Sciences
Pallavi V. Vetal, Yves Poirier
Summary: Inorganic phosphate (Pi) homeostasis is crucial for plant growth and relies on the transport of Pi across plant cells. A protein called PHO1 plays a role in this transport process, but its localization on the plasma membrane does not align with its function. By inhibiting a process called clathrin-mediated endocytosis (CME), researchers discovered that PHO1 can be re-localized to the plasma membrane, which affects its transport function.
Article
Plant Sciences
Ning Zhang, Olga A. Zabotina
Summary: Glycosyltransferases (GTs) play a critical role in synthesizing various glycans in cells. Glycosylation occurs in the endoplasmic reticulum and Golgi, requiring the delivery of related enzymes to the appropriate locations. This review summarizes the protein trafficking mechanism between the endoplasmic reticulum and Golgi.
Article
Chemistry, Medicinal
Xin-Ya Xu, Siu Wai Tsang, Yi-Fu Guan, Kang-Lun Liu, Wen-Hui Pan, Chu Shing Lam, Kwan Ming Lee, Yi-Xuan Xia, Wen-Jian Xie, Wing Yan Wong, Magnolia Muk Lan Lee, William Chi Shing Tai, Hong-Jie Zhang
JOURNAL OF MEDICINAL CHEMISTRY
(2019)
Review
Biochemical Research Methods
Brandon Dow Chan, Wing-Yan Wong, Magnolia Muk-Lan Lee, William Chi-Shing Cho, Benjamin K. Yee, Yiu Wa Kwan, William Chi-Shing Tai
Article
Biophysics
Ying Xin, Xi Chen, Xin Tang, Keming Li, Mo Yang, William Chi-Shing Tai, Yiyao Liu, Youhua Tan
BIOPHYSICAL JOURNAL
(2019)
Article
Chemistry, Organic
Mengxun Zhang, Magnolia Muk-Lan Lee, Weijian Ye, Wing-Yan Wong, Brandon Dow Chan, Sibao Chen, Lizhi Zhu, William Chi-Shing Tai, Chi-Sing Lee
JOURNAL OF ORGANIC CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Rui Liu, Brandon Dow Chan, Daniel Kam-Wah Mok, Chi-Sing Lee, William Chi-Shing Tai, Sibao Chen
Article
Agricultural Engineering
Rui Tang, Yanbing Shen, Menglei Xia, Linna Tu, Jianmei Luo, Yuhan Geng, Tian Gao, Haijie Zhou, Yunqiu Zhao, Min Wang
BIORESOURCE TECHNOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Ang-Xin Song, Yu-Heng Mao, Ka-Chai Siu, William Chi Shing Tai, Jian-Yong Wu
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2019)
Article
Chemistry, Applied
Qinyuan Ma, Xiuzhen Gao, Xinyu Bi, Qi Han, Linna Tu, Yupeng Yang, Yanbing Shen, Min Wang
CARBOHYDRATE POLYMERS
(2020)
Review
Pathology
Yin Lam Chu, Harriet Li, Pik Lan Amanda Ng, Siu Ting Kong, Hao Zhang, Yusheng Lin, William Chi Shing Tai, Allen Chi Shing Yu, Aldrin Kay Yuen Yim, Hin Fung Tsang, William Chi Shing Cho, Sze Chuen Cesar Wong
EXPERT REVIEW OF MOLECULAR DIAGNOSTICS
(2020)
Article
Immunology
Brandon Dow Chan, Gabriella Wong, Qing Jiang, Magnolia Muk-Lan Lee, Wing-Yan Wong, Feifei Chen, Wing-Tak Wong, Lixing Zhu, Francis Kim-Ming Wong, William Chi-Shing Tai
MICROBIAL PATHOGENESIS
(2020)
Article
Plant Sciences
Jian-Shu Lou, Li-Ping Zhao, Zhi-Hui Huang, Xia-Yin Chen, Jing-Ting Xu, William Chi-Shing Tai, Karl W. K. Tsim, Yi-Tao Chen, Tian Xie
Summary: The study found that ginkgetin derived from Ginkgo biloba leaves enhances DDP-induced cytotoxicity in NSCLC cells through inducing ferroptosis. The results indicate that, in addition to enhancing DDP-induced cytotoxicity, ginkgetin can disrupt redox homeostasis in DDP-treated cells and enhance apoptosis.
Article
Food Science & Technology
Yanfang Wu, Menglei Xia, Nan Zhao, Linna Tu, Danni Xue, Xianglong Zhang, Cuimei Zhao, Yang Cheng, Yu Zheng, Min Wang
Summary: This study used metatranscriptomics to explore the metabolic profile of main organic acids in traditional Chinese cereal vinegar produced using solid-state fermentation. The results revealed that carbon metabolism is dominant during fermentation, with shifts in metabolic pathways of acetic acid and lactic acid at different stages, predominantly contributed by Lactobacillus and Acetobacter. Temperature and acetic acid were identified as environmental factors regulating metabolic activity during solid-state fermentation.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Microbiology
Qinyuan Ma, Xiuzhen Gao, Linna Tu, Qi Han, Xing Zhang, Yabo Guo, Wenqin Yan, Yanbing Shen, Min Wang
FRONTIERS IN MICROBIOLOGY
(2020)
Article
Chemistry, Multidisciplinary
Shiying Li, Kwok-Ho Lui, Tik-Hung Tsoi, Wai-Sum Lo, Xin Li, Xuesen Hu, William Chi-Shing Tai, Clara Hiu-Ling Huang, Yan-Juan Gu, Wing-Tak Wong
NANOSCALE ADVANCES
(2019)
Article
Biochemistry & Molecular Biology
Qiuyue Lv, Yushan Lin, Zhexu Tan, Baoping Jiang, Lijia Xu, Haiqin Ren, William Chi-shing Tai, Chi-on Chan, Chi-sing Lee, Zhengbing Gu, Daniel K. W. Mok, Sibao Chen