Article
Immunology
Chao Niu, Yongchong Chen, Min Li, Shan Zhu, Lei Zhou, Dongsheng Xu, Zhaozhi Li, Jianting Xu, Wei Li, Yufeng Wang, Jiuwei Cui
Summary: The study found that rituximab can independently obtain highly cytotoxic NK cells from PBMCs, without the need for additional feeder cells, and these cells have stronger antitumor activity against cancer cells, depending on autologous living B cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Vera Vorstandlechner, Dragan Copic, Katharina Klas, Martin Direder, Bahar Golabi, Christine Radtke, Hendrik J. Ankersmit, Michael Mildner
Summary: This study demonstrates the anti-fibrotic effect of PBMCsec on cutaneous scars in both mouse and human experimental settings. PBMCsec regulates the expression of fibrosis-related genes, attenuates myofibroblast differentiation, and inhibits the breakdown of elastic fibers. These findings suggest that PBMCsec could be a novel therapeutic option for treating skin scarring.
Article
Materials Science, Multidisciplinary
Byeongyeon Kim, In-Ho Seo, Gwang Myeong Seo, Woo-Joong Kim, Eui-Cheol Shin, Sungyoung Choi
Summary: The study introduces a blood divider (B-DV) as a centrifuge tube insert to prevent RBC contamination during PBMC harvest, simplifying the separation process and enabling rapid, easy PBMC isolation.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Geriatrics & Gerontology
Chung-Sheng Lee, Chin-Hao Chang, Chin-Ying Chen, Chih-Yuan Shih, Jen-Kuei Peng, Hsien-Liang Huang, Pei-Yun Chen, Tse-Le Huang, Ching-Yu Chen, Jaw-Shiun Tsai
Summary: The study found that the expression levels of CD36 were up-regulated in frail older adults and positively correlated with the frailty status. This suggests that CD36 may serve as a potential biomarker for frailty severity.
JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE
(2022)
Article
Medicine, General & Internal
Alessia Scatena, Pasquale Petruzzi, Filippo Maioli, Francesca Lucaroni, Cristina Ambrosone, Giorgio Ventoruzzo, Francesco Liistro, Danilo Tacconi, Marianna Di Filippi, Nico Attempati, Leonardo Palombi, Leonardo Ercolini, Leonardo Bolognese
Summary: The study showed that treatment with autologous PBMNC implants in NO-CLI patients with DFUs significantly reduced amputation rate, improved survival, and promoted wound healing. Autologous PBMNC injection may effectively prevent amputations in patients with DFUs.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Lidiane Cristina Pinho Nascimento Portela, Thiago Barbosa Cahu, Thais Santos Bezerra, Dayane Kelly Dias do Nascimento Santos, Georon Ferreira Sousa, Rogerio William Santos Portela, Cristiane Moutinho Lagos Melo, Ranilson de Souza Bezerra
Summary: This study investigated the immunostimulatory effects of collagen and chitosan preparations extracted from fishery discards on human peripheral blood mononuclear cells (PBMCs). The results showed that these preparations did not exhibit cytotoxic effects, but they promoted the release of cytokines and increased the production of cellular ROS. They also led to the activation of T CD8(+) and CD4(+) lymphocytes, as well as an increase in cytosolic calcium concentration and mitochondrial membrane potential.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biochemistry & Molecular Biology
Belay Tessema, Janine Riemer, Ulrich Sack, Brigitte Koenig
Summary: The results of CSA parameters measured in PBMCs are influenced by the PBMC isolation method, age, seasonal variation, and gender. Researchers and physicians should properly interpret the results of CSA parameters in PBMCs by considering these factors. Separate CSA evaluation standards should be used based on the isolation method, age, gender, and season-dependent factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Keiichiro Yoshioka, Hironori Sato, Takeshi Kawasaki, Daisuke Ishii, Takuro Imamoto, Mitsuhiro Abe, Yoshinori Hasegawa, Osamu Ohara, Koichiro Tatsumi, Takuji Suzuki
Summary: RNA sequencing analysis revealed differential gene expression patterns in peripheral blood mononuclear cells (PBMCs) between patients with pulmonary sarcoidosis and healthy controls, with 270 differentially expressed genes involved in various biological processes related to the pathobiology of sarcoidosis. Enrichment analysis of gene ontology and KEGG pathways indicated the potential involvement of specific signaling pathways in sarcoidosis pathogenesis, providing insight into potential therapeutic targets for the disease.
FRONTIERS IN MEDICINE
(2022)
Article
Microbiology
Mohammad Enamul Hoque Kayesh, Md Abul Hashem, Fumie Maetani, Atsushi Goto, Noriko Nagata, Aki Kasori, Tetsuya Imanishi, Kyoko Tsukiyama-Kohara
Summary: This study investigated the immune responses of captive koalas infected with multiple KoRV subtypes. The results showed that CD4 and CD8b mRNA expression in koala PBMCs were not significantly affected by different KoRV subtypes. Although there was a slight difference in IFN-gamma mRNA expression in koala PBMCs infected with different subtypes, the difference was not statistically significant. Additionally, IL-6 and IL-10 mRNA expression did not vary significantly in koala PBMCs based on KoRV subtype differences. The study also found significant differential expression of TLR5, 7, 9, 10, and 13 mRNA in koala PBMCs infected with different KoRV subtypes.
Article
Nutrition & Dietetics
Efrossini Briassouli, Marianna Tzanoudaki, Dimitris Goukos, Kostas Vardas, Panagiotis Briassoulis, Stavroula Ilia, Maria Kanariou, Christina Routsi, Serafim Nanas, George L. Daikos, George Briassoulis
Summary: The study aimed to evaluate the effects of lipopolysaccharide (LPS) or heat shock (HS) induction and glutamine modulation on heat shock protein-90 alpha (HSP90α) and cytokines in peripheral blood mononuclear cells (PBMCs). Heat shock increased HSP90α mRNA and mHSP90α in all groups, while LPS induced mHSP90α and lHSP90α protein expression. Glutamine enhanced LPS-induction of MCP-1 in all groups. Future research should further investigate the impact of glutamine and temperature modulation on HSP90α and MCP-1 pathways in sepsis and trauma.
Article
Biochemistry & Molecular Biology
Josefina Atrisco-Morales, Monica Ramirez, Carlos Alberto Castanon-Sanchez, Adolfo Roman-Roman, Ilce Valeria Roman-Fernandez, Dinorah Nashely Martinez-Carrillo, Samuel Garcia-Arellano, Jose Francisco Munoz-Valle, Hugo Alberto Rodriguez-Ruiz, Gloria Fernandez-Tilapa
Summary: This study analyzed the cytokine profile secreted by H. pylori-infected human peripheral blood mononuclear cells (PBMCs) and established a network of interaction between cytokines and cellular proteins. The results showed that H. pylori infection significantly increased the secretion of IL-1 beta, IL-6, TNF-alpha, IL-10, IL-17A, IL-21, and IL-22 in both soluble and exosomal forms. The protein-protein interaction network indicated that these cytokines interact with proteins involved in signaling pathways related to carcinogenesis. Cytokine-loaded exosomes may play a role in inflammation-related extragastrointestinal diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Torsten Lowin, Sofia Anna Laaser, Christina Kok, Eileen Bruneau, Georg Pongratz
Summary: CBD has pro- and anti-inflammatory effects on B cells and PBMCs, depending on the activating stimulus and concentration of CBD. Therefore, CBD may be used to dampen B cell activity in autoimmune conditions such as rheumatoid arthritis.
CANNABIS AND CANNABINOID RESEARCH
(2023)
Article
Oncology
Jacobo Rogado, Fernando Pozo, Kevin Troule, Jose Miguel Sanchez-Torres, Nuria Romero-Laorden, Rebeca Mondejar, Olga Donnay, Anabel Ballesteros, Vilma Pacheco-Barcia, Javier Aspa, Fatima Al-Shahrour, Arantzazu Alfranca, Ramon Colomer
Summary: Peripheral blood mononuclear cells (PBMCs) have been explored as potential biomarkers to predict the efficacy of anti-PD-1 immunotherapy in advanced non-small cell lung cancer (NSCLC) patients. High baseline levels of circulating T cell subpopulations related to tissue lymphocyte recruitment were found to be associated with poorer outcomes in immunotherapy-treated advanced NSCLC patients.
Article
Cell Biology
Abhirup Bagchi, Aneesha Nath, Vasanth Thamodaran, Smitha Ijee, Dhavapriya Palani, Vignesh Rajendiran, Vigneshwaran Venkatesan, Phaneendra Datari, Aswin Anand Pai, Nancy Beryl Janet, Poonkuzhali Balasubramanian, Yukio Nakamura, Alok Srivastava, Kumarasamypet Murugesan Mohankumar, Saravanabhavan Thangavel, Shaji R. Velayudhan
Summary: This study established a protocol for culturing early-stage EPCs from peripheral blood and immortalizing them by expressing HEE genes, providing a new cellular model for studying the molecular mechanisms of human erythropoiesis. The two iEPCs generated showed stable doubling times and erythroid progenitor properties, suitable for studying therapeutic targets and mechanisms of red cell diseases.
Article
Biology
Iliya Shulman, Sergei Ogurcov, Alexander Kostennikov, Alexander Rogozin, Ekaterina Garanina, Galina Masgutova, Mikhail Sergeev, Albert Rizvanov, Yana Mukhamedshina
Summary: The therapeutic potential of peripheral blood mononuclear cells (PBMCs) in a pig spinal cord injury model was evaluated, showing partial restoration of spinal cord conduction function after application. The distribution of transplanted PBMCs in the injured spinal cord was mainly in the grey matter, contributing to an increase in tissue integrity and partially restored conduction along posterior columns. Cytokine imbalance was observed in blood serum and cerebrospinal fluid without significant shift towards pro-inflammatory or anti-inflammatory cytokine cascades.
Article
Obstetrics & Gynecology
Milosz Wilczynski, Ewelina Zytko, Justyna Danielska, Bozena Szymanska, Monika Dzieniecka, Marek Nowak, Jakub Malinowski, Dariusz Owczarek, Jacek R. Wilczynski
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2018)
Article
Oncology
Katarzyna Bednarska-Szczepaniak, Damian Krzyianowski, Magdalena Klink, Marek Nowak
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2019)
Review
Biochemistry & Molecular Biology
Michal Kielbik, Izabela Szulc-Kielbik, Magdalena Klink
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2019)
Article
Microbiology
Michal Kielbik, Izabela Szulc-Kielbik, Magdalena Klink
FEMS MICROBIOLOGY LETTERS
(2019)
Article
Cell Biology
Izabela Szulc-Kielbik, Michal Kielbik, Patrycja Przygodzka, Anna Brzostek, Jaroslaw Dziadek, Magdalena Klink
MEDIATORS OF INFLAMMATION
(2019)
Review
Cell Biology
Marek Nowak, Magdalena Klink
Article
Oncology
Milosz Wilczynski, Michal Kielbik, Daria Senderowska, Tomasz Krawczyk, Bozena Szymanska, Magdalena Klink, Jan Bienkiewicz, Hanna Romanowicz, Filip Fruhauf, Andrzej Malinowski
Article
Immunology
Izabela Szulc-Kielbik, Anna Brzostek, Justyna Gatkowska, Michal Kielbik, Magdalena Klink
IMMUNOLOGY LETTERS
(2020)
Article
Biochemistry & Molecular Biology
Michal Kielbik, Izabela Szulc-Kielbik, Magdalena Klink
Summary: SNAIL1, rather than SNAIL2, is implicated in cisplatin resistance of ovarian cancer cells. STAT3 serves as a universal regulator for the expression of SNAIL1/2 in ovarian cancer cells, regardless of their chemoresistance or invasive capabilities. The expression of SNAIL1 and N-cadherin is associated with cisplatin resistance, while SNAIL2 and E-cadherin levels are related to sensitivity to cisplatin in ovarian cancer cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Michal Kielbik, Izabela Szulc-Kielbik, Magdalena Klink
Summary: Immunogenic cell death (ICD), characterized by features of necroptosis and apoptosis, plays a crucial role in eliminating tumor cells through inducing an adaptive immune response. The focus of the research is on the involvement of CRT in ICD and its expression on ovarian cancer cells, as well as its prognostic and predictive value in ovarian cancer patients.
Review
Biochemistry & Molecular Biology
Izabela Szulc-Kielbik, Michal Kielbik, Marek Nowak, Magdalena Klink
Summary: IL-6 plays multiple roles in ovarian cancer, including promoting cell proliferation, inhibiting apoptosis, enhancing cell migration and invasion, and correlating with chemotherapy resistance. Its elevated levels in serum and ascites of ovarian cancer patients suggest it as a potential biomarker for distinguishing malignant and nonmalignant tumors and predicting chemotherapy response. IL-6 also serves as a potential target in anti-cancer therapy.
BIOCHIMICA ET BIOPHYSICA ACTA-REVIEWS ON CANCER
(2021)
Review
Biochemistry & Molecular Biology
Michal Kielbik, Izabela Szulc-Kielbik, Magdalena Klink
Summary: The loss/reduction of E-cadherin expression on tumor cells is associated with the migration, invasiveness, and metastasis of cancer cells. It is also an indicator of cancer cells' aggressiveness. This study evaluates the expression of E-cadherin in primary ovarian cancer tissue in relation to patient survival, disease stage, tumor grade, histopathological type, and differentiation between malignant and nonmalignant ovarian tumors. The analysis of existing literature suggests that reduced E-cadherin expression is associated with shorter overall survival and advanced disease stage in epithelial ovarian carcinoma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Oncology
M. M. Zyla, M. Nowak, T. Stetkiewicz, M. Kostrzewa, K. Ksiezakowska-Lakoma, M. Wilczynski, J. Danielska, J. R. Wilczynski
EUROPEAN JOURNAL OF GYNAECOLOGICAL ONCOLOGY
(2018)
Article
Immunology
Hilal Uensal, Canan Caka, Hacer Neslihan Bildik, Saliha Esenboga, Alphan Kupesiz, Baris Kuskonmaz, Duygu Uckan Cetinkaya, Mirjam van Der Burg, Ilhan Tezcan, Deniz Cagdas
Summary: The study investigated the demographic, clinical, laboratory, follow-up, and treatment characteristics of 21 patients with Major Histocompatibility Complex Class II (MHC-II) deficiency. It was found that patients with Bare Lymphocyte Syndrome (BLS) exhibited clinical findings similar to severe combined immunodeficiency (SCID), and the flow cytometric MHC-II expression study played a crucial role in the diagnosis, differential diagnosis, early hematopoietic stem cell transplantation (HSCT), and post-HSCT follow-up.
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2024)
Article
Immunology
Axel Rosell, Cecilia Karlstrom, Joakim S. Dahlin, Daryl Boey, Monika Klimkowska, Kajsa Ax, Charlotte Thalin, Johanna Ungerstedt
Summary: There is no evidence of global increase in neutrophil extracellular trap release in patients with systemic mastocytosis.
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2024)
Article
Immunology
Charles W. Armitage, Connor P. O'Meara, Emily R. Bryan, Avinash Kollipara, Logan K. Trim, Danica Hickey, Alison J. Carey, Wilhelmina M. Huston, Gavin Donnelly, Anusch Yazdani, Richard S. Blumberg, Kenneth W. Beagley
Summary: This study demonstrates that IgG seroconversion during male infection can amplify female immunopathology and infertility. IgG transcytosis through the FcRn receptor allows Chlamydia to cross the epithelial barrier and be phagocytosed by antigen-presenting cells. This leads to increased pro-inflammatory signaling and T cell proliferation, resulting in more severe pathology and infertility in female mice.
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2024)
Article
Immunology
Fatma Gonca Kocanci, Azize Yasemin Goksu Erol, Fatma Yildiz, Hamiyet Eciroglu
Summary: Calcineurin inhibitors, such as pimecrolimus, have been found to have preventive effects against neuroinflammation and apoptotic pathways, which are underlying mechanisms in neurodegenerative diseases. This study demonstrated that pimecrolimus exerts neuroprotective effects by inhibiting inflammation and oxidative stress, making it a potential treatment option for neurodegenerative diseases.
SCANDINAVIAN JOURNAL OF IMMUNOLOGY
(2024)
