Recognition of fluoride anions at low ppm level inside living cells and from fluorosis affected tooth and saliva samples

A simple Schiff base chemosensor 2-((2-(2,4-dinitro phenyl) hydrazono) methyl)-4-nitrophenol (L) has been developed as a colorimetric and fluorimetric 'turn on' sensor for fluoride (F-). F- recognition at ppm levels from mouth rinses and a toothpaste water solution has been successful. Significantly, L can detect F- from fluorosis affected tooth and saliva samples by similar colorimetric changes. A test kit for F- detection from a DMSO-water (1 : 1) mixture is also engineered. Intracellular F- from pollen grains of Techoma stans and Candida albicans (a diploid fungus), grown in 10(-6) (M) F- contaminated water has been successfully detected under a fluorescence microscope.