期刊
RUSSIAN JOURNAL OF PLANT PHYSIOLOGY
卷 60, 期 1, 页码 149-154出版社
PLEIADES PUBLISHING INC
DOI: 10.1134/S1021443712060118
关键词
Nicotiana tabacum; chilling tolerance; mechanical stimulation; proline; tobacco suspension cells
资金
- Natural Science Foundation of Yunnan Province of China [2010ZC066]
- Education Department Foundation of Yunnan Province of China [09Y0145]
Mechanical stimulation (MS), widely existing but usually ignored in nature, is one of the major environmental stress factors. MS by increasing the rotational speed of shaker incubator could alleviate a decrease in vitality of tobacco (Nicotiana tabacum L.) suspension cultured cells and reduce the accumulation of MDA under chilling stress at 1A degrees C, which in turn improved survival percentage under chilling stress and regrowth ability of tobacco suspension cells after chilling stress. In addition, MS could increase the activity of Delta(1)-pyrroline-5-carboxylate synthetase (P5CS) and induce the accumulation of endogenous proline in tobacco cells; exogenously applied proline also could enhance its endogenous level under normal culture conditions and survival percent-age of the cells under chilling stress. These results suggest that MS could improve chilling tolerance of tobacco suspension cells and the acquisition of this chilling tolerance was related to proline.
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