Article
Chemistry, Applied
Jie Chen, Qing Kong, Ziting Sun, Jiayuan Liu
Summary: The study using liquid chromatography-mass spectrometry found that lipid composition in salmon muscle changes over time when stored at 4 degrees Celsius, with four lipids showing significant increase from the tenth day. Traditional freshness index also indicated spoilage of salmon slices by the tenth day, suggesting they may serve as indicators of raw salmon freshness.
Article
Biochemistry & Molecular Biology
Siddabasave Gowda B. Gowda, Chen Yifan, Divyavani Gowda, Yui Tsuboi, Hitoshi Chiba, Shu-Ping Hui
Summary: This study compared the lipid profiles of five commonly consumed Japanese dietary seaweeds using non-targeted liquid chromatography/mass spectrometry (LC/MS). The results showed distinct lipid molecular compositions in different seaweeds, with kombu found to contain high levels of antioxidants and glycerophospholipids (GPs) and glycerolipids (GLs). Dietary seaweeds have the potential to be antioxidants and health-promoting foods due to their high levels of PUFA-rich GPs and GLs.
Article
Biochemical Research Methods
Eva-Maria Harrieder, Fleming Kretschmer, Sebastian Boecker, Michael Witting
Summary: Metabolomics and lipidomics involve the large-scale analysis of metabolites, requiring advanced analytical methods. Liquid Chromatography-Mass Spectrometry (LC-MS) is a commonly used technique that provides different selectivities in separation and high sensitivity in detection. Due to the huge chemical diversity, there is no single analysis method that can cover the entire range of metabolites or lipids, leading to the use of different separation methods. This review explores the current use of LC-MS in metabolomics and lipidomics using data from public databases, and highlights potential future trends and improvements needed.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Chemistry, Analytical
Huan Wu, Hui Cheng, Shengyong Luo, Can Peng, An Zhou, Zhiwu Chen, Hong Wu, Qinglin Li
Summary: The study demonstrates that HCS injection inhibits cell proliferation and induces apoptosis in HepG2 cells, as well as exhibits significant anti-HCC effects in an H22 hepatoma-bearing mouse model. Through UPLC-HRMS-based cellular metabolomics and lipidomics, 25 potential biomarkers associated with the intervention effect of HCS were identified, with UDP-GlcNAc and glutathione being confirmed. Metabolic pathway analysis revealed the involvement of glutathione, cysteine and methionine metabolism, sphingolipid metabolism, fatty acid biosynthesis, amino sugar, and nucleotide sugars in the anti-HCC effects of HCS injection.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Yuting Wang, Yisheng Wang, Chen Chen, Fang Ren, Rui Cao, Yuefei Wang, Pin Han, Xiaoyan Zhang, Congjian Xu, Xinyu Liu, Guowang Xu
Summary: The study identified lipid disturbances in the serum of patients with epithelial ovarian cancer (EOC) and established 4 lipids as potential markers for aiding EOC diagnosis. These markers showed high accuracy in distinguishing EOC and early EOC from non-cancer, with good specificity and sensitivity. The research not only revealed the characteristics of lipid metabolism in EOC, but also provided a potential marker pattern for aiding EOC diagnosis.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Domenico Masuero, Domen Skrab, Giulia Chitarrini, Mar Garcia-Aloy, Pietro Franceschi, Paolo Sivilotti, Graziano Guella, Urska Vrhovsek
Summary: A new mass spectrometry method was developed in this study, successfully analyzing 412 compounds in grapes. The results showed that certain lipid components were positively correlated with grape maturity, while others were negatively correlated.
Article
Biochemistry & Molecular Biology
Alex Apffel, Limian Zhao, Mark J. Sartain
Summary: This article introduces a novel solid-phase extraction method for extracting lipids from human plasma, which saves time and labor compared to traditional liquid-liquid extraction methods, and provides equivalent or better qualitative and quantitative results in terms of recovery, reproducibility, and lipidome coverage.
Article
Oncology
Jun Li, Ting Zhao, Jing Li, Jiechen Shen, Li Jia, Bojing Zhu, Liuyi Dang, Chen Ma, Didi Liu, Fan Mu, Liangshuo Hu, Shisheng Sun
Summary: This study identified numerous unique N-glycan structures in ICC through precision N-glycoproteomic analysis, providing a valuable resource for future research on ICC diagnostic biomarkers, therapeutic targets, and mechanism investigations.
MOLECULAR ONCOLOGY
(2022)
Article
Microbiology
Nicole J. Bale, Su Ding, Ellen C. Hopmans, Milou G. I. Arts, Laura Villanueva, Christine Boschman, Andreas F. Haas, Stefan Schouten, Jaap S. Sinninghe Damste
Summary: Utilizing ultra-high-pressure liquid chromatography coupled with high-resolution mass spectrometry to conduct untargeted analysis of MS1 spectral data on environmental microbial communities is a valuable approach. By treating each mass spectrum as a component and clustering them based on similarity in abundance depth profiles, this rapid method can visualize component distributions and identify novel lipid biomarkers.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Iku Sazaki, Toshihiro Sakurai, Arisa Yamahata, Sumire Mogi, Nao Inoue, Koutaro Ishida, Ami Kikkai, Hana Takeshita, Akiko Sakurai, Yuji Takahashi, Hitoshi Chiba, Shu-Ping Hui
Summary: Oxidized low-density lipoproteins (oxLDLs) induce oxidative stress in liver tissue, leading to hepatic steatosis, inflammation, and fibrosis. This study investigated the effects of oxLDL on lipid metabolism, lipid droplet formation, and gene expression in a human liver cell line. The results showed that oxLDL increased lipid droplets enriched with CE hydroperoxides (CE-OOH) and altered the expression of certain genes involved in lipid metabolism. These findings suggest that oxLDL may play a crucial role in the development of NAFLD and NASH and could be a potential therapeutic target and biomarker for these diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Isin T. Sakallioglu, Amith S. Maroli, Aline De Lima Leite, Robert Powers
Summary: This study introduces a rapid reversed-phase ultra-high-performance liquid chromatography-high resolution mass spectrometry method for mycobacterial lipidomics analysis, which achieves separation of various lipid classes specific to mycobacterium in a short runtime of 14 minutes. The method demonstrates high sensitivity and faster sample throughput compared to existing methods.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Oncology
Vincent Tambay, Valerie-Ann Raymond, Corentine Goossens, Louise Rousseau, Simon Turcotte, Marc Bilodeau
Summary: This study aimed to reveal the metabolic signature of hepatocellular carcinoma compared to adjacent normal liver cells using targeted and non-targeted metabolomics. The results showed distinct metabolite signatures between both sample types, supporting the concept of metabolic reprogramming in hepatocellular carcinoma.
Article
Oncology
Ning Liu, Yaokun Wu, Yunyun Tao, Jing Zheng, Xiaohua Huang, Lin Yang, Xiaoming Zhang
Summary: This study investigated the efficacy of MRI radiomics in differentiating HCC from ICC. The results suggest that MRI-based radiomics may help noninvasively differentiate HCC from ICC, and the model integrating radiomics features and clinical risk factors showed a further improvement in performance.
Article
Chemistry, Analytical
Na Yang, Wei Hu, Jun He, Xu Wu, Ting Zou, Jiahui Zheng, Chongbo Zhao, Min Wang
Summary: In this study, a lipidomic strategy was used to investigate the lipidomic patterns of high-fat diet-induced hyperlipidemic hamsters when treated with Polygonum cuspidatum. The results showed that Polygonum cuspidatum improved the lipidomic profile of hyperlipidemia. A total of 65 differential lipids related to the hypolipidemic effect of Polygonum cuspidatum were screened out and identified.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Chemistry, Analytical
Eliska Ivanovova, Barbora Pisklakova, Dana Dobesova, Ales Kvasnicka, David Friedecky
Summary: Diagnosing a large group of inborn errors of metabolism poses a challenge for physicians due to non-specific clinical symptoms, leading to the importance of laboratory methods for differential diagnosis. Liquid chromatography and mass spectrometry (LC-MS) technology has significantly expanded diagnostic possibilities in the last two decades. It has been used for multicomponent analysis of metabolites, as well as successfully applied in metabolomics and lipidomics to discover new potential biomarkers.
MICROCHEMICAL JOURNAL
(2021)
