Article
Medicine, Research & Experimental
Fatemeh Yazarlou, Sepideh Kadkhoda, Soudeh Ghafouri-Fard
Summary: Let-7 is a family of miRNA that plays a crucial role in various hematological malignancies by influencing the expression of oncogenic pathways. The expression of these miRNAs varies between different hematological malignancies, highlighting their importance in disease treatment and prognosis. This review summarizes the data on biogenesis, genomic locations, targets, and regulatory networks of Let-7 in the context of hematological malignancies.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Endocrinology & Metabolism
Jiahui Song, Haining Lv, Beibei Liu, Mingjun Hao, Hugh S. Taylor, Xuchen Zhang, Da Li, Yingqun Huang
Summary: The study reveals the important role of let-7 in liver fibrosis progression. It inhibits the production of TGF-I31 and expression of FAS, thereby reducing hepatocyte apoptosis and liver fibrosis. The findings highlight the potential use of let-7 and its derivatives as therapeutic agents for liver fibrosis.
MOLECULAR METABOLISM
(2023)
Article
Oncology
Maximillian S. Westphal, Eunjee Lee, Eric E. Schadt, Giselle S. Sholler, Jun Zhu
Summary: This study categorized medulloblastoma into molecular subgroups and found different survival rates among them. By analyzing the expression of molecular markers MYCN and let-7, one subgroup was further divided and it was found that high MYCN expression and high let-7 activity are associated with worse overall survival. These findings provide critical information for personalized treatment options for medulloblastoma.
Review
Biochemistry & Molecular Biology
Ya Wang, Juanjuan Zhao, Shipeng Chen, Dongmei Li, Jing Yang, Xu Zhao, Ming Qin, Mengmeng Guo, Chao Chen, Zhixu He, Ya Zhou, Lin Xu
Summary: Abnormal regulation and expression of microRNA, especially let-7, are closely related to the aging process and aging-related diseases. Let-7 plays a crucial role in development and is elevated in aging tissues, participating in multiple pathways that regulate aging. Recent studies also suggest that let-7 may be involved in aging by regulating immune function.
Article
Entomology
Yu Fu, Yi Wang, Qunxia Huang, Chenyue Zhao, Xinmei Li, Yunchao Kan, Dandan Li
Summary: This study found that lncRNA lncR17454 in silkworm is involved in the metamorphosis process through the let-7 miRNA cluster and the ecdysone signaling pathway, providing new clues for lepidopteran pest control.
JOURNAL OF INSECT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Chun Li, Bohyung Yoon, Giovanni Stefani, Frank J. Slack
Summary: This study reveals a novel role for lipid kinase PPK-1/PIP5K1A in regulating miRNA levels. PPK-1/PIP5K1A is able to control the levels of let-7 miRNA in C. elegans and human cells. It interacts with nuclear export protein XPO5 to block the binding of XPO5 to pre-let-7 miRNA, thus regulating the levels of mature miRNA.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Joshua Miguel C. Danac, Reynaldo L. Garcia
Summary: Circular RNAs play important roles in disease processes. Studies have shown that circPVT1 is involved in cancer progression, and its knockdown can reduce the survival and migration abilities of cancer cells.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Seong Heon Kim, Bi-Oh Park, Kidae Kim, Byoung Chul Park, Sung Goo Park, Jeong-Hoon Kim, Sunhong Kim
Summary: This study revealed that Sjogren Syndrome antigen B (SSB) protein interacts with LIN28A and LIN28B in the nucleus, regulating the LIN28-let-7 axis and impacting developmental timing control.
BIOCHIMICA ET BIOPHYSICA ACTA-GENE REGULATORY MECHANISMS
(2021)
Review
Biochemistry & Molecular Biology
Amit Chaudhary, Pankaj Kumar Chaurasia, Sandeep Kushwaha, Pallavi Chauhan, Aakash Chawade, Ashutosh Mani
Summary: Cold shock proteins are a conserved family of proteins that play important roles in low-temperature stress response and nucleic acid binding in bacteria. They are involved in various cellular and metabolic processes in prokaryotes and have been found to be crucial in the progression of cancer and Alzheimer's disease in humans.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Entomology
Asli Dageri, Mohammed Lengichow Kadir, Nurper Guz, Ayhan Ogreten, Muhammad Arshad
Summary: In this study, two cold-regulated genes, TgAFP and TgCSDP, were identified in T. granarium. The expression of these genes in response to temperature and cold stress was examined. The results suggest that these genes play crucial roles in the cold survival and adaptation of T. granarium.
JOURNAL OF STORED PRODUCTS RESEARCH
(2023)
Article
Oncology
Hanmin Wang, Evgeny Chirshev, Nozomi Hojo, Tise Suzuki, Antonella Bertucci, Michael Pierce, Christopher Perry, Ruining Wang, Jeffrey Zink, Carlotta A. Glackin, Yevgeniya J. Ioffe, Juli J. Unternaehrer
Summary: The study investigated the mechanism of epithelial-mesenchymal transition (EMT)-induced stemness in cancer cells, revealing the crucial role of the SNAI1/let-7 axis in stemness pathways. The results provide a rationale for future research on targeting this axis for cancer stem cell-specific therapies.
Article
Microbiology
Ekaterina Pushkareva, Josef Elster, Burkhard Becker
Summary: Microorganisms in Antarctic biocrusts have developed adaptations to survive extreme conditions, with dominant presence of cyanobacteria, actinobacteria, and proteobacteria. Metagenomic sequencing revealed high abundance of cold shock domain A proteins and presence of cold shock proteins and antifreeze proteins in these environments. The majority of identified cold shock proteins were Csp A, C, and E, with proteobacteria and actinobacteria as the primary sources for CsdA, Csp, and AFP.
Article
Microbiology
Liqun Wang, Tingli Liu, Guoliang Chen, Yanping Li, Shaohua Zhang, Li Mao, Panhong Liang, Majid Fasihi Harandi, Taoshan Li, Xuenong Luo
Summary: The study reveals that miR-let-7-5p released by Taenia pisiformis cysticercus plays a critical role in activating M2 macrophages, inducing M2 macrophage polarization by targeting C/EBP delta.
Article
Biochemistry & Molecular Biology
Lydia Borgelt, Fubao Huang, Lisa Hohnen, Xiaqiu Qiu, Georg L. L. Goebel, Pascal Hommen, Peng Wu
Summary: A new LIN28 inhibitor has been discovered, which disrupts the interaction between LIN28 and let-7 miRNA, inhibiting LIN28 expression and cancer cell proliferation. This finding provides a potential candidate for the development of next-generation LIN28 inhibitors.
Review
Oncology
Udo Heinemann, Yvette Roske
Summary: The cold-shock domain is a conserved protein domain with a simple architecture but complex biological functions, found from bacteria to humans, that can bind both DNA and RNA through non-specific molecular interactions. The structural properties of cold-shock domains allow them to function in transcription regulation, RNA splicing, translation, stability, and sequestration, primarily through promiscuous DNA and RNA binding.
Article
Cell Biology
Murali Palangat, Dimitrios G. Anastasakis, Dennis Liang Fei, Katherine E. Lindblad, Robert Bradley, Christopher S. Hourigan, Markus Hafner, Daniel R. Larson
GENES & DEVELOPMENT
(2019)
Article
Oncology
Bruna Rodrigues Muys, Josane F. Sousa, Jessica Rodrigues Placa, Luiza Ferreira de Araujo, Aishe A. Sarshad, Dimitrios G. Anastasakis, Xiantao Wang, Xiao Ling Li, Greice Andreotti de Molfetta, Anelisa Ramao, Ashish Lal, Daniel Onofre Vidal, Markus Hafner, Wilson A. Silva
Article
Cell Biology
Saifeng Wang, Bryan Chim, Yijun Su, Pavel Khil, Madeline Wong, Xiantao Wang, Amir Foroushani, Patrick T. Smith, Xiuhuai Liu, Rui Li, Sundar Ganesan, Chrysi Kanellopoulou, Markus Hafner, Stefan A. Muljo
GENES & DEVELOPMENT
(2019)
Article
Multidisciplinary Sciences
Stephan P. Rosshart, Jasmin Herz, Brian G. Vassallo, Ashli Hunter, Morgan K. Wall, Jonathan H. Badger, John A. McCulloch, Dimitrios G. Anastasakis, Aishe A. Sarshad, Irina Leonardi, Nicholas Collins, Joshua A. Blatter, Seong-Ji Han, Samira Tamoutounour, Svetlana Potapova, Mark B. Foster St Claire, Wuxing Yuan, Shurjo K. Sen, Matthew S. Dreier, Benedikt Hild, Markus Hafner, David Wang, Iliyan D. Iliev, Yasmine Belkaid, Giorgio Trinchieri, Barbara Rehermann
Article
Oncology
Irina A. Elcheva, Tyler Wood, Kathryn Chiarolanzio, Bryan Chim, Madeline Wong, Vikash Singh, Chethana P. Gowda, Qingli Lu, Markus Hafner, Sinisa Dovat, Zhenqiu Liu, Stefan A. Muljo, Vladimir S. Spiegelman
Article
Multidisciplinary Sciences
Pooja Sharma, Sarah Alsharif, Karina Bursch, Swetha Parvathaneni, Dimitrios G. Anastasakis, Joeffrey Chahine, Arwa Fallatah, Kevin Nicolas, Sudha Sharma, Markus Hafner, Bhaskar Kallakury, Byung Min Chung
SCIENTIFIC REPORTS
(2019)
Article
Biotechnology & Applied Microbiology
Min Guo, Yue Li, Yijun Su, Talley Lambert, Damian Dalle Nogare, Mark W. Moyle, Leighton H. Duncan, Richard Ikegami, Anthony Santella, Ivan Rey-Suarez, Daniel Green, Anastasia Beiriger, Jiji Chen, Harshad Vishwasrao, Sundar Ganesan, Victoria Prince, Jennifer C. Waters, Christina M. Annunziata, Markus Hafner, William A. Mohler, Ajay B. Chitnis, Arpita Upadhyaya, Ted B. Usdin, Zhirong Bao, Daniel Colon-Ramos, Patrick La Riviere, Huafeng Liu, Yicong Wu, Hari Shroff
NATURE BIOTECHNOLOGY
(2020)
Article
Multidisciplinary Sciences
Amir K. Foroushani, Bryan Chim, Madeline Wong, Andre Rastegar, Patrick T. Smith, Saifeng Wang, Kent Barbian, Craig Martens, Markus Hafner, Stefan A. Muljo
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Multidisciplinary Sciences
Rebecca S. Brown, Dimitrios G. Anastasakis, Markus Hafner, Margaret Kielian
NATURE COMMUNICATIONS
(2020)
Article
Biochemistry & Molecular Biology
Dimitrios G. Anastasakis, Alexis Jacob, Parthena Konstantinidou, Kazuyuki Meguro, Duncan Claypool, Pavol Cekan, Astrid D. Haase, Markus Hafner
Summary: CLIP methods, particularly PAR-CLIP, allow for the interrogation of direct protein-RNA interactions and gene regulatory networks. This technique utilizes photoreactive nucleosides to crosslink interacting molecules, offering a high sensitivity and specificity in pinpointing RNA binding sites.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biotechnology & Applied Microbiology
Yutaka Enomoto, Peng Li, Lisa M. Jenkins, Dimitrios Anastasakis, Gaelyn C. Lyons, Markus Hafner, Warren J. Leonard
Summary: NK-EVs derived from IL-15 + IL-21 stimulated NK-92 cells show enhanced cytotoxic capacity against tumor cells, with CD226 playing a key role. However, enrichment of cytolytic granules granzyme B and granzyme H is not the key factor contributing to the enhanced cytotoxic activity.
CANCER GENE THERAPY
(2022)
Article
Cell Biology
Mark Sauer, Nina Was, Thomas Ziegenhals, Xiantao Wang, Markus Hafner, Matthias Becker, Utz Fischer
Summary: The miR-26 family acts as a trigger for a self-amplifying system required for neural differentiation by directly targeting Rest, inducing the expression of neuronal genes, and halting neurogenesis at the neural progenitor level. This identification sheds light on the molecular events triggered by miR-26 in regulating neurogenesis.
JOURNAL OF CELL SCIENCE
(2021)
Article
Multidisciplinary Sciences
Dan Cheng, Kevin Semmens, Elizabeth McManus, Qingrong Chen, Daoud Meerzaman, Xiantao Wang, Markus Hafner, Brian A. Lewis, Hidehisa Takahashi, Ballachanda N. Devaiah, Anne Gegonne, Dinah S. Singer
Summary: TAF7, a component of TFIID, is identified as a previously unknown RNA chaperone that contributes to the regulation of protein synthesis by binding a broad spectrum of target RNA species and delivering them to cytoplasmic polysomes. The export of TAF7-bound RNAs to the cytoplasm is dependent on a TAF7 nuclear export signal and an exportin 1-dependent pathway. Disruption of either TAF7's RNA binding or its nuclear export leads to retention of target messenger RNAs in the nucleus and reduced protein levels of TAF7-target RNAs.
Article
Biochemistry & Molecular Biology
Yingxiao Chen, Yanping Li, Roselyn S. Dai, Jonathan C. Savage, Ujwal Shinde, John Klimek, Larry L. David, Emma A. Young, Markus Hafner, Rosalie C. Sears, Xiao-Xin Sun, Mu-Shui Dai
Summary: The RNA exosome is regulated by the nucleolar ubiquitin-specific protease USP36 through direct interaction with the exonuclease EXOSC10 in the nucleolus. USP36 promotes the SUMOylation of EXOSC10 at lysine (K) 583, which is critical for the function of the RNA exosome in ribosome biogenesis.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Multidisciplinary Sciences
Markus Hafner, Maria Katsantoni, Tino Koester, James Marks, Joyita Mukherjee, Dorothee Staiger, Jernej Ule, Mihaela Zavolan
Summary: This article discusses the process of RNA assembly into RNP complexes and introduces the techniques for identifying the binding sites of RNA-binding proteins through CLIP and other methods. It also covers the challenges of computational CLIP data analysis, handling background sources, and identifying binding regions, as well as the prospect of integrating different methods to gain a comprehensive view.
NATURE REVIEWS METHODS PRIMERS
(2021)