Review
Biochemistry & Molecular Biology
Paola De Magistris
Summary: Nuclear export of mRNA through the NPC is crucial for protein translation in eukaryotic cells, with mRNAs forming ribonuclear particles with proteins for translocation. mRNPs undergo compositional and conformational changes during NPC transport, with multiple quality control steps to ensure proper export of mature transcripts to the cytoplasm. Opportunities for mechanistic understanding of nuclear export are mentioned in the review.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Oncology
Katherine L. B. Borden
Summary: This review highlights the importance of RNA export in cellular trafficking and the aberrant phenomena observed in cancer. Studies on RNA export machinery and relevant NPC components offer potential therapeutic strategies for targeting cancer.
Article
Multidisciplinary Sciences
Belen Pacheco-Fiallos, Matthias K. Vorlaender, Daria Riabov-Bassat, Laura Fin, Francis J. O'Reilly, Farja I. Ayala, Ulla Schellhaas, Juri Rappsilber, Clemens Plaschka
Summary: Newly made mRNAs are recognized and organized by the essential TREX complex for nuclear export. Cryo-electron microscopy and tomography structures reveal that mRNAs are recognized through multivalent interactions between TREX subunit ALYREF and mRNP-bound exon junction complexes. Endogenous mRNPs form compact globules that are coated by multiple TREX complexes, providing a framework to understand mRNA biogenesis and export.
Article
Biotechnology & Applied Microbiology
Qiucheng Yao, Mengdi Zhang, Shaopo Zu, Hong Yang, Weitian Xie, Jinjun Chen, Zhibao Chen, Ye Ge, Weiwei Zeng, Zhihui Zhao
Summary: Grass carp hemorrhagic disease, caused by grass carp reovirus, is a fatal disease. Aberrant regulation of transcripts has been implicated in various diseases. Through RNA sequencing, differentially expressed miRNAs and mRNAs were identified in grass carp fibroblasts infected with virulent GCRVs. Integrated analysis highlighted immune response and cell death as the most affected processes.
MARINE BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Merce Gomar-Alba, Vasilisa Pozharskaia, Bogdan Cichocki, Celia Schaal, Arun Kumar, Basile Jacquel, Gilles Charvin, J. Carlos Igual, Manuel Mendoza
Summary: Nuclear pore complexes (NPCs) mediate communication between the nucleus and the cytoplasm and regulate gene expression. Lysine acetyltransferase Esa1 acetylates the nuclear pore basket component Nup60, which recruits the mRNA export factor Sac3, to promote mRNA export. This mechanism also regulates cell entry into S phase and prevents premature commitment to a new cell division cycle in G1 daughter cells.
Article
Biochemistry & Molecular Biology
Zhiling Kuang, Jiyuan Ke, Jiong Hong, Zhongliang Zhu, Liwen Niu
Summary: PCI domain proteins play important roles in post-transcriptional gene regulation. In this study, the structure of yeast Thp3-Csn12-Sem1 ternary complex was determined, revealing significant structural differences compared to the Sac3-Thp1-Sem1 complex. This structure provides a foundation for further exploring its specific recruitment to the spliceosome.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Cell Biology
Minhua Deng, Ning Wang, Zhiyong Li, Rixin Chen, Jinling Duan, Yulu Peng, Zeshen Wu, Zhiling Zhang, Lijuan Jiang, Xianchong Zheng, Dan Xie, Wensu Wei, Zhuowei Liu, Fangjian Zhou
Summary: RNA-binding proteins (RBPs), including Fragile X-related gene 1 (FXR1), play important regulatory roles in gene expression. Dysregulation of RBPs, such as overexpression of FXR1, can promote urothelial carcinoma of the bladder (UCB) proliferation and tumorigenesis by affecting the 3' processing of TRAF1 mRNA. Understanding the novel regulatory role of FXR1 in UCB oncogenesis provides new insight into the function of RBPs and potential therapeutic targets for UCB.
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Suji Lim, Yan Liu, Byung-Ho Rhie, Chun Kim, Hong-Yeoul Ryu, Seong Hoon Ahn
Summary: The study finds that Sus1 is a key factor in linking transcription and mRNA nuclear export. SAGA DUB module and TREX-2 have distinct roles in yeast replicative lifespan. Loss of Sus1 affects growth and lifespan, which depends on TREX-2. Increasing mRNA export factors can rescue the effects.
Article
Virology
Margarita Valdes Aleman, Luca D. Bertzbach, Thomas Speiseder, Wing Hang Ip, Ramon A. Gonzalez, Thomas Dobner
Summary: During human adenovirus infection, the late phase of viral replication results in a significant increase in viral late mRNA abundance, which outcompetes cellular mRNA biogenesis rather than selectively exporting viral mRNA.
Article
Biochemistry & Molecular Biology
Anna Uzonyi, David Dierks, Ronit Nir, Oh Sung Kwon, Ursula Toth, Isabelle Barbosa, Cindy Burel, Alexander Brandis, Walter Rossmanith, Herve Le Hir, Boris Slobodin, Schraga Schwartz
Summary: N6-methyladenosine (m6A) is a widespread destabilizing mark on mRNA with non-uniform distribution across the transcriptome. In this study, the authors propose that m6A deposition is exclusion-based rather than selective. They demonstrate that m6A consensus motifs are methylated by default unless they are near splice junctions. By validating a simple model based on the presence of m6A motifs and exon-intron architecture, they show that mRNA decay is mechanistically mediated via m6A.
Article
Biochemistry & Molecular Biology
Tinghan Li, Yibo Wen, Hangtian Guo, Tingting Yang, Haitao Yang, Xiaoyun Ji
Summary: The study investigates the role and mechanism of accessory protein Orf6 in the inhibition of host interferon signaling in coronaviruses. Orf6 interacts with the Rae1-Nup98 complex to inhibit the nuclear export of mRNA. The results show that SARS-CoV Orf6 occupies the potential mRNA-binding groove of the Rae1-Nup98 complex, and the highly conserved methionine (M58) in SARS-CoVs Orf6 plays a critical role.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Microbiology
Laura White, Bilgi Erbay, G. Eric Blair
Summary: Cajal bodies can be reorganized into microfoci during adenovirus infection. The protein p80-coilin forms a complex with L4-22K protein, facilitating the nuclear export of Ad mRNA.
Article
Oncology
Dandan Li, Li Shen, Xudong Zhang, Zhen Chen, Pan Huang, Congcong Huang, Shanshan Qin
Summary: This study reveals the SNAI2-ELF3-AS1 feedback loop that regulates the expression of ELF3 at transcriptional and post-transcriptional levels, driving gastric cancer metastasis by maintaining SNAI2 overexpression. Additionally, the study provides direct evidence that head-to-head antisense lncRNAs can share promoters with neighboring coding genes, resulting in high co-expression.
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
M. M. Kurshakova, D. Kopytova, S. G. Georgieva
Summary: The TREX-2 protein complex, essential for mRNA export, interacts with the ORC complex. Specifically, the Xmas-2 protein serves as a platform for TREX-2 assembly and interacts with Orc3, requiring a C-terminal region of Xmas-2.
DOKLADY BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Cell Biology
Kun Li, Dongbin Zhang, Shuiting Zhai, Huilin Wu, Hongzhi Liu
Summary: This study aimed to identify whether the m6A writer METTL3/METTL4 regulates rip3 mRNA expression in AAA. The results showed that interference with METTL3/METTL14 attenuated VSMC necroptosis, inflammatory response, and the AAA pathological process in vivo.
JOURNAL OF CELL COMMUNICATION AND SIGNALING
(2023)
Article
Biochemistry & Molecular Biology
S. Saran, D. D. H. Tran, F. Ewald, A. Koch, A. Hoffmann, M. Koch, B. Nashan, T. Tamura
Review
Oncology
Doan Duy Hai Tran, Shashank Saran, Alexandra Koch, Teruko Tamura
Article
Cell Biology
Doan Duy Hai Tran, Alexandra Koch, Shashank Saran, Marcel Armbrecht, Florian Ewald, Martina Koch, Tom Wahlicht, Dagmar Wirth, Armin Braun, Bjoern Nashan, Matthias Gaestel, Teruko Tamura
CELLULAR SIGNALLING
(2016)
Article
Cell Biology
Doan Duy Hai Tran, Alexandra Koch, Aldrige Allister, Shashank Saran, Florian Ewald, Martina Koch, Bjoern Nashan, Teruko Tamura
CELLULAR SIGNALLING
(2016)
Article
Biochemistry & Molecular Biology
D. D. H. Tran, C. Kessler, S. E. Niehus, M. Mahnkopf, A. Koch, T. Tamura
Article
Cell Biology
Alexandra Koch, Shashank Saran, Doan Duy Hai Tran, Sabine Klebba-Faerber, Hauke Thiesler, Katherina Sewald, Susann Schindler, Armin Braun, Robert Klopfleisch, Teruko Tamura
CELL COMMUNICATION AND SIGNALING
(2014)
Review
Cell Biology
Doan D. H. Tran, Alexandra Koch, Teruko Tamura
CELL COMMUNICATION AND SIGNALING
(2014)
Article
Cell Biology
Svenja Ellen Niehus, Doan Duy Hai Tran, Michaela Mischak, Alexandra Koch
CELLULAR SIGNALLING
(2018)
Article
Oncology
F. Griaud, A. Pierce, M. B. Gonzalez Sanchez, M. Scott, S. A. Abraham, T. L. Holyoake, D. D. H. Tran, T. Tamura, A. D. Whetton
Article
Biochemistry & Molecular Biology
Doan Duy Hai Tran, Shashank Saran, Andrew J. K. Williamson, Andrew Pierce, Oliver Dittrich-Breiholz, Lutz Wiehlmann, Alexandra Koch, Anthony D. Whetton, Teruko Tamura
NUCLEIC ACIDS RESEARCH
(2014)
Article
Multidisciplinary Sciences
Svenja E. Niehus, Aldrige B. Allister, Andrea Hoffmann, Lutz Wiehlmann, Teruko Tamura, Doan Duy Hai Tran
SCIENTIFIC REPORTS
(2019)
Article
Biochemistry & Molecular Biology
Colin F. Davenport, Tobias Scheithauer, Alessia Dunst, Frauke Sophie Bahr, Marie Dorda, Lutz Wiehlmann, Doan Duy Hai Tran
Summary: Downregulation of multiple tumor suppressor genes is crucial in cancer formation, with epigenetic modifications playing a significant role. Using nanopore sequencing technology, the study identified potential TSGs in HCC and demonstrated the tumor suppressor function of GCK in inhibiting HCC cell proliferation through lactate accumulation-induced energy crisis. These findings offer valuable insights for further understanding tumorigenesis in human cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Sebastian Burbano De Lara, Doan Duy Hai Tran, Aldrige Bernardus Allister, Mareike Polenkowski, Bjoern Nashan, Martina Koch, Teruko Tamura
Summary: This study identifies C20orf204-189AA as a cancer type-specific fine tuner in HCC, enhancing cell proliferation and ribosomal RNA transcription, and interacting with ribosomal RNA and nucleolin. The expression of C20orf204-189AA correlates with tumor differentiation grade and patient survival in HCC.
Article
Biochemistry & Molecular Biology
Mareike Polenkowski, Sebastian Burbano de Lara, Aldrige Bernardus Allister, Thi Nhu Quynh Nguyen, Teruko Tamura, Doan Duy Hai Tran
Summary: Identification of cancer-specific lncRNAs and micropeptides can contribute to novel treatment and immunotherapeutic strategies. In this study, we found a HCC-specific lncRNA encoding a micropeptide that enhances transcription of ribosomal RNA and supports growth of HCC. We also discovered novel micropeptides originating from HCC-specific lncRNAs. One of these micropeptides, Linc013026-68AA, is expressed in a subset of HCC cells and plays a role in cell proliferation. Our findings shed light on the importance of the "dark proteome" in cancer maintenance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Mareike Polenkowski, Aldrige Bernardus Allister, Sebastian Burbano de Lara, Andrew Pierce, Bethany Geary, Omar El Bounkari, Lutz Wiehlmann, Andrea Hoffmann, Anthony D. Whetton, Teruko Tamura, Doan Duy Hai Tran
Summary: THOC5 is essential for the 3'processing of inducible genes, mRNA export, and stem cell survival. Depletion of THOC5 leads to altered mRNA 3'cleavage and changes in RNA polymerase II binding across genes. THOC5 is recruited near high-density polymerase II sites, suggesting its involvement in transcriptional elongation.