Article
Crystallography
Jason C. Grigg, Ian R. Price, Ailong Ke
Summary: This study demonstrates that tRNA fusion can simplify the crystallization and structure determination of RNA molecules. The approach was applied to the T-box riboswitch system, capturing the dynamic interaction between tRNA and the T-box antiterminator.
Article
Materials Science, Multidisciplinary
Kakeru Obayashi, Kazutaka Kamitani, Chien-Wei Chu, Ryosuke Kawatoko, Chao-Hung Cheng, Atsushi Takahara, Ken Kojio
Summary: The internal structure of polyurethane adhesive undergoes changes during lap shear deformation, with an increase in the spacing of hard segment domains and the merging of tilt angle with the stretching direction. These findings are of great significance for the practical design of adhesives.
ACS APPLIED POLYMER MATERIALS
(2022)
Article
Engineering, Biomedical
Yi Zhang, Wenkai Zhang, Tim Snow, Ying Ju, Yang Liu, Andrew J. Smith, Sujay Prabakar
Summary: This study investigated the changes in structure and stability of ABPs during crosslinking using GA or EDC at different concentrations through synchrotron small-angle X-ray scattering and thermal and colorimetric methods. The findings reveal that telopeptidyl-helical linkages are preferred at low crosslinker concentrations, while excess crosslinking leads to structural changes and plateauing of stabilities.
ACTA BIOMATERIALIA
(2022)
Article
Chemistry, Physical
O. R. Santos, D. Reis, A. G. Oliveira-Filho, C. L. P. Oliveira, A. M. Figueiredo Neto
Summary: This study investigates a lyotropic mixture in the calamitic nematic phase and its corresponding calamitic cholesteric phase with the addition of a chiral agent. The structure and local ordering at the nanoscale were analyzed using Small-Angle X-ray Scattering. The results show that the overall shape of the micelles is not modified by the presence of the chiral agent, but the correlation between micelles in the cholesteric phase is increased.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Review
Chemistry, Physical
Kun Qian, Randall E. Winans, Tao Li
Summary: Understanding the nanostructures of liquid electrolytes is crucial for enhancing electrochemical energy storage capacities, but the solvation structures and molecular dynamics in electrolytes are difficult to probe. Small-angle X-ray scattering (SAXS) offers unique insights into molecular clusters and ion pairs in electrolytes, with excellent time-resolution for studying molecular dynamics. This review focuses on the microscopic understanding of liquid electrolytes by SAXS and highlights the unique capabilities of X-ray scattering for nanostructure characterization.
ADVANCED ENERGY MATERIALS
(2021)
Article
Medicine, Research & Experimental
Dileep Urimi, Maja Hellsing, Najet Mahmoudi, Christopher Soderberg, Ronja Widenbring, Lars Gedda, Katarina Edwards, Thorsteinn Loftsson, Nicolaas Schipper
Summary: Lipid nanocapsules (LNCs) with core-shell structure were studied using various techniques, and it was found that drug loading does not alter the overall structure of LNCs. However, the presence of the drug leads to an increase in the thickness of the shell while the core size remains unchanged.
MOLECULAR PHARMACEUTICS
(2022)
Article
Chemistry, Applied
Guantian Li, Yacine Hemar, Fan Zhu
Summary: The molecular and granular structure of 9 quinoa starches were investigated and found that short amylopectin chains were positively correlated with the thickness of crystalline lamellae, while long internal chains were positively correlated with the thickness of amorphous lamellae. The study also suggested that the current chain classification lacks a strong structural basis for the supramolecular organization of granules.
FOOD HYDROCOLLOIDS
(2021)
Article
Chemistry, Multidisciplinary
Ezzeldin Metwalli, Klaus Goetz, Tobias Zech, Christian Baer, Isabel Schuldes, Anne Martel, Lionel Porcar, Tobias Unruh
Summary: A customized portable SAXS instrument has been constructed and tested at the D22 SANS instrument at ILL, with optimized lead shielding arrangement and double energy threshold X-ray detector to suppress high-energy gamma radiation background. The upgraded SAXS instrument allows for improved signal-to-noise ratio data and combined SAXS and SANS measurements, enabling simultaneous fitting of SAXS and SANS data from the same sample volume for unambiguous interpretation of scattering data. Advanced in situ/real time investigations are now possible, revealing time-resolved complementary nanoscale structural information.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Catia Barria, Diogo Athayde, Guillem Hernandez, Leonor Fonseca, Jorge Casinhas, Tiago N. Cordeiro, Margarida Archer, Cecilia M. Arraiano, Jose A. Brito, Rute G. Matos
Summary: Ribonucleases play a crucial role in RNA regulation by processing, degrading, and quality controlling cellular transcripts. Campylobacter jejuni, the main cause of human gastroenteritis, relies on the exoribonuclease PNPase (CjPNP) for low-temperature survival, virulence factor synthesis, and swimming, cell adhesion/invasion, and chick colonization abilities. The crystallographic structure of CjPNP, along with SAXS analysis, confirms its trimeric arrangement and provides insight into domain arrangement and flexibility. Mutations in highly conserved residues reveal unexpected RNA degradation activity even under conditions favoring polymerization. These findings have significant implications for the development of strategies to combat C. jejuni infections.
Article
Multidisciplinary Sciences
David N. Azulay, Oliver Spaeker, Mnar Ghrayeb, Michaela Wilsch-Brauninger, Ernesto Scoppola, Manfred Burghammer, Ivo Zizak, Luca Bertinetti, Yael Politi, Liraz Chai
Summary: Biofilms are multicellular microbial communities encased in an extracellular matrix, with phenotypic heterogeneity enhancing their survival. The molecular structures, elemental distribution, and possible linkages within biofilms remain unexplored, showcasing the importance of molecular-level heterogeneity in shaping biofilm physiology and development.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Biochemistry & Molecular Biology
Ying Li, Qike Li, Richard E. Gillilan, Alireza Abbaspourrad
Summary: This study reveals the reversible modulation of protein-protein and protein-chromophore interactions of Cphycocyanin (C-PC) from Spirulina platensis under hydrostatic pressure. The results show that high pressures can disassemble C-PC into monomers or detached subunits, accompanied by protein unfolding. The pressure-induced changes in structure and color can be precisely manipulated and reversed. This knowledge can be used to design new delivery and optical materials.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Pranay Amruth Maroju, Ramakrishnan Ganesan, Jayati Ray Dutta
Summary: In this study, a facile solution-based method was used to surface derivatize poly(& epsilon;-caprolactone) films and electrospun fibers with lysozyme, aiming to impart antimicrobial properties and examine the impact on enzymatic degradation. The derivatized films and fibers showed high antibacterial efficacy against Escherichia coli and Staphylococcus aureus. The degradation rate experienced a slight decrease upon lysozyme derivatization, but this reduction was effectively countered by the inclusion of Tween-20.
MACROMOLECULAR BIOSCIENCE
(2023)
Article
Chemistry, Physical
Pierre Toudret, Julie Wolanin, Gerard Gebel, Arnaud Morin
Summary: In this work, the hydration of the ionomer in both the membrane and the catalyst layers of a proton exchange membrane fuel cell was quantified using small angle X-ray scattering operando measurements. The study found that water content increases during current generation, with water concentration increasing from anode to cathode.
JOURNAL OF POWER SOURCES
(2023)
Review
Cell Biology
Tao Wang, Xiaojun Li, Xiaojing Zhang, Qing Wang, Wenqian Liu, Xiaohong Lu, Shunli Gao, Zixi Liu, Mengshuang Liu, Lihong Gao, Wenna Zhang
Summary: A large number of RNA molecules have been found in the phloem of higher plants, where specific RNA motifs and structures facilitate their mobility, while RNA methylation, such as m5C, plays a crucial role in RNA transport. These studies provide a theoretical foundation for uncovering the mechanism of RNA transportation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chinmayee Dash, Ramyaranjan Das, Deepak K. Sahu, Divyansh Upreti, T. Umasankar Patro, Dillip K. Bisoyi
Summary: The present study aims to establish a correlation between macromolecular parameters obtained from small angle X-ray scattering (SAXS) analysis of pretreated sunn hemp fibers and the dielectric and mechanical properties of their composites. Different pretreatment methods, including dewaxing, alkalization, and microwave irradiation, were used to modify the structure of the fibers, which in turn influenced the macromolecular parameters. The modified fibers showed improved mechanical and electrical properties in the reinforced composites.
Article
Biochemistry & Molecular Biology
John A. Hammond, Rajan Lamichhane, David P. Millar, James R. Williamson
JOURNAL OF MOLECULAR BIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Pedro Serrano, John A. Hammond, Michael Geralt, Kurt Wuthrich
Article
Biochemistry & Molecular Biology
John A. Hammond, Li Zhou, Rajan Lamichhane, Hui-Yi Chu, David P. Millar, Larry Gerace, James R. Williamson
JOURNAL OF MOLECULAR BIOLOGY
(2018)
Article
Microbiology
Pierrick Craveur, Anna T. Gres, Karen A. Kirby, Dandan Liu, John A. Hammond, Yisong Deng, Stefano Forli, David S. Goodsell, James R. Williamson, Stefan G. Sarafianos, Arthur J. Olson
Article
Multidisciplinary Sciences
Jingxin Wang, John Hammond, Kristen A. Johnson
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2019)
Meeting Abstract
Biochemistry & Molecular Biology
Elizabeth Spear, Megan Filbin-Wong
Article
Biochemistry & Molecular Biology
Yisong Deng, John A. Hammond, Raymond Pauszek, Stosh Ozog, Ilean Chai, Jessica Rabuck-Gibbons, Rajan Lamichhane, Scott C. Henderson, David P. Millar, Bruce E. Torbett, James R. Williamson
Summary: Most GCC species are mainly composed of monomeric or dimeric Gag molecules bound to ribosomal complexes, representing a population of Gag not yet functionally committed for incorporation into viable virion precursors. A subset of large GCC complexes, with more than 20 Gag molecules, may be equivalent to membrane-associated puncta as genuine assembling-virus intermediates. These findings provide a rationale for the existence of diverse GCCs and lay the foundation for characterizing on-pathway intermediates in early virus assembly.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Meeting Abstract
Biochemistry & Molecular Biology
Julie Y. Lee, Liheng Zhou, Laura D. Baquero-Galvis, Megan E. Filbin
Meeting Abstract
Biochemistry & Molecular Biology
Liheng Zhou, Julie Lee, Laura D. Baquero-Galvis, Megan E. Filbin
Meeting Abstract
Biochemistry & Molecular Biology
Nathan L. Nowling, Elizabeth A. Spear, Natasia Paukovich, John Manalastas, Beat Vogeli, Megan E. Filbin
Meeting Abstract
Biochemistry & Molecular Biology
John Manalastas, Elizabeth Spear, Megan Filbin
Meeting Abstract
Biochemistry & Molecular Biology
Megan E. Filbin-Wong, Tamir Gonen, Jeffrey S. Kieft
Meeting Abstract
Biochemistry & Molecular Biology
Laura D. Baquero Galvis, Elizabeth Shields, Evan J. Morrison, Megan E. Filbin-Wong
Meeting Abstract
Biochemistry & Molecular Biology
Megan E. Filbin-Wong, Tamir Gonen, Jeffrey S. Kieft
Meeting Abstract
Biochemistry & Molecular Biology
Scott-Wesley M. Bean, Laura D. Baquero Galvis, Evan J. Morrison, Megan E. Filbin-Wong