Article
Biochemistry & Molecular Biology
Michal Mayer, Lulu Winer, Amihai Karniel, Elhanan Pinner, Eliane H. Yardeni, David Morgenstern, Eitan Bibi
Summary: Many integral membrane proteins are produced by translocon-associated ribosomes. Our studies reveal the co-translational targeting of FtsY and its interactions with cytosolic chaperones and the translocon, providing insights into the mechanism of membrane targeting.
JOURNAL OF MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Shivangi, Mary Krishna Ekka, Laxman S. Meena
Summary: This study explores the essential factors affecting the enzymatic activity of FtsY, including its hydrolysis kinetics of GTP, the influence of cations like Mg2+ and Mn2+ on activity, and the structural changes of FtsY in response to ligand binding. Mutational analysis showed that mutations at key residues significantly reduce FtsY's GTPase activity, while MD simulation indicated effective binding and stabilization of FtsY with GTP, GDP and ATP.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
Sayandeep Gupta, Mousam Roy, Dhritiman Dey, Koustav Bhakta, Arghya Bhowmick, Dhrubajyoti Chattopadhyay, Abhrajyoti Ghosh
Summary: The study demonstrates the important role of archaeal 7S RNA in stabilizing the SRP54$FtsY targeting complex, with further assistance from SRP19. These results suggest that archaeal 7S RNA and SRP19 reinforce the targeting complex to execute protein translocation through modulation of its conformation.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Shivangi, Laxman S. Meena
Summary: The study highlights the important role of FtsY in mycobacterial growth, with inhibitors ML141 and NAV2729 identified as potential tools to constrain the SRP pathway.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Joao P. L. Coelho, Sichen Shao
Summary: A recent study reveals an unexpected role of ribosome-bound nascent chain-associated complex in sensing ER-targeting signals and recruiting SRP for accurate protein targeting.
TRENDS IN BIOCHEMICAL SCIENCES
(2022)
Article
Cell Biology
Pinku Sarmah, Wenkang Shang, Andrea Origi, Mariya Licheva, Claudine Kraft, Maximilian Ulbrich, Elisabeth Lichtenberg, Annegret Wilde, Hans -Georg Koch
Summary: Signal-sequence-dependent protein targeting is crucial for the organization of eukaryotic and prokaryotic cells, facilitated by factors like the signal recognition particle. However, targeting signals can also be present within mRNAs. In this study, the authors demonstrated that mRNA targeting is regulated by nucleotide content and secondary structures within mRNAs. Importantly, membrane insertion of proteins translated from membrane-bound mRNAs occurs independently of the SRP pathway, providing an alternative strategy for protein targeting when the SRP pathway is compromised.
Article
Biochemistry & Molecular Biology
Joori Park, Jeeyoon Chang, Hyun Jung Hwang, Kwon Jeong, Hyuk-Joon Lee, Hongseok Ha, Yeonkyoung Park, Chunghun Lim, Jae-Sung Woo, Yoon Ki Kim
Summary: This study identified a surveillance pathway in which pioneer translation ensures proper targeting of endoplasmic reticulum and mitochondrial proteins, preventing abnormal protein folding and cytosolic stress response.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Jan Pyrih, Tomas Panek, Ignacio Miguel Durante, Vendula Raskova, Kristyna Cimrhanzlova, Eva Kriegova, Anastasios D. Tsaousis, Marek Elias, Julius Lukes
Summary: This study reveals the presence of homologs of bacterial Ffh and FtsY proteins in various plastid-lacking unicellular eukaryotes, suggesting they constitute parts of an ancestral mitochondrial signal peptide-based protein-targeting system inherited from the last eukaryotic common ancestor. This system appears to have been lost from the majority of extant eukaryotes, with implications for protein targeting in different organisms.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Review
Immunology
Tongtong Zhang, Wei Hu, Wei Chen
Summary: The plasma membrane serves as a critical platform for immune cells to initiate signaling cascades and immune responses against pathogens or tumor cells. The biophysical-chemical properties of the plasma membrane may cooperatively regulate immune receptor functions, impacting their structural conformation, distribution, and organization to influence signal transduction.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Tingting Guo, Yue Cui, Lingwen Zhang, Xiaoning Xu, Zhenxiang Xu, Jian Kong
Summary: This study demonstrates that phage-encoded holin can be used to enhance extracellular protein yields in both Gram-negative and -positive bacteria. Overexpression of holin increases the secretion of target proteins and suggests an alternative pathway for protein secretion.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Multidisciplinary Sciences
Romain Linares, Charles-Adrien Arnaud, Gregory Effantin, Claudine Darnault, Nathan Hugo Epalle, Elisabetta Boeri Erba, Guy Schoehn, Cecile Breyton
Summary: The structures of the T5 tail tip before and after interaction with its receptor, FhuA, have been determined using cryo-electron microscopy. These structures reveal important conformational changes, such as bending of the central fiber, anchoring of the tail to the membrane, opening of the tail tube, and formation of a transmembrane channel. This provides detailed insights into the initial steps of the infection mechanism.
Review
Oncology
Xia Gan, Yonghong Liu, Xueni Wang
Summary: Glioblastomas, a type of brain tumor, have a higher incidence in males compared to females, and this difference may be attributed to the androgen receptor signaling axis. Drug research targeting the androgen receptor for the treatment of glioblastoma has shown that androgen receptor antagonists, in combination with other drugs, can have anti-tumor effects. Additionally, small molecule compounds targeting the androgen receptor have also shown promising results in reducing glioblastoma growth.
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Stanislav S. Piletsky, Ekaterina Baidyuk, Elena V. Piletska, Larissa Lezina, Konstantin Shevchenko, Donald J. L. Jones, Thong H. Cao, Rajinder Singh, Alan C. Spivey, Eric O. Aboagye, Sergey A. Piletsky, Nickolai A. Barlev
Summary: Molecularly imprinted polymer nanoparticles (nanoMIPs) have shown potential as an alternative to antibodies in diagnostic and therapeutic applications. Targeting intracellular epitopes can modulate protein function, making them a new tool for cancer therapy.
Article
Medicine, Research & Experimental
Chang Lu, Qiuyang Zhang, Huiying Zhang, Xiumiao Li, Qin Jiang, Jin Yao
Summary: Anlotinib has shown promise in inhibiting ocular neovascularization by reducing avascular areas and pathological neovascular tufts in animal models, suggesting its potential as a therapeutic candidate for eye diseases in the future.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Biochemistry & Molecular Biology
Elizabeth E. Haywood, Nicholas B. Handy, James W. Lopez, Mengfei Ho, Brenda A. Wilson
Summary: Bacterial protein toxins can be utilized for targeted cytosolic delivery of therapeutic cargo, with efficient endosomal escape being a critical factor. The cytotoxic necrotizing factor family of toxins serves as a useful model for investigating cargo-delivery mechanisms.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Biochemistry & Molecular Biology
Cristina Maracci, Marina V. Rodnina
Article
Multidisciplinary Sciences
Niels Fischer, Piotr Neumann, Lars V. Bock, Cristina Maracci, Zhe Wang, Alena Paleskava, Andrey L. Konevega, Gunnar F. Schroeder, Helmut Grubmueller, Ralf Ficner, Marina V. Rodnina, Holger Stark
Article
Biochemistry & Molecular Biology
Riccardo Belardinelli, Heena Sharma, Neva Caliskan, Carlos E. Cunha, Frank Peske, Wolfgang Wintermeyer, Marina V. Rodnina
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2016)
Review
Biochemistry & Molecular Biology
Marina V. Rodnina
Editorial Material
Biochemistry & Molecular Biology
Riccardo Belardinelli, Heena Sharma, Frank Peske, Wolfgang Wintermeyer, Marina V. Rodnina
Article
Multidisciplinary Sciences
Takayuki Katoh, Ingo Wohlgemuth, Masanobu Nagano, Marina V. Rodnina, Hiroaki Suga
NATURE COMMUNICATIONS
(2016)
Article
Biochemistry & Molecular Biology
Riccardo Belardinelli, Heena Sharma, Neva Caliskan, Carlos E. Cunha, Frank Peske, Wolfgang Wintermeyer, Marina V. Rodnina
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2016)
Article
Cell Biology
Heena Sharma, Sarah Adio, Tamara Senyushkina, Riccardo Belardinelli, Frank Peske, Marina V. Rodnina
Article
Chemistry, Multidisciplinary
Namit Ranjan, Marina V. Rodnina
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2017)
Article
Biochemistry & Molecular Biology
Neva Caliskan, Ingo Wohlgemuth, Natalia Korniy, Michael Pearson, Frank Peske, Marina V. Rodnina
Review
Biology
Marina V. Rodnina, Niels Fischer, Cristina Maracci, Holger Stark
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2017)
Article
Multidisciplinary Sciences
Xabier Agirrezabala, Ekaterina Samatova, Mariia Klimova, Miguel Zamora, David Gil-Carton, Marina V. Rodnina, Mikel Valle
Review
Biochemistry & Molecular Biology
Marija Liutkute, Ekaterina Samatova, Marina V. Rodnina
Article
Biochemistry & Molecular Biology
Michael Thommen, Wolf Holtkamp, Marina V. Rodnina
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2017)
Review
Cell Biology
Namit Ranjan, Marina V. Rodnina