期刊
RNA BIOLOGY
卷 10, 期 11, 页码 1661-1669出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/rna.26851
关键词
P-body assembly; eIF2; gephyronic acid A; inhibitors; myxobacterial metabolites; processing bodies; stress granules
资金
- Spanish Ministerio de Ciencia e Innovacion [BFU2010-20803, SAF2020-21336]
- Deutsche Forschungsgemeinschaft [SA 356/7-1]
- ICREA Funding Source: Custom
Processing bodies (P-bodies) are cytoplasmatic mRNP granules containing non-translating mRNAs and proteins from the mRNA decay and silencing machineries. The mechanism of P-body assembly has been typically addressed by depleting P-body components. Here we apply a complementary approach and establish an automated cell-based assay platform to screen for molecules affecting P-body assembly. From a unique library of compounds derived from myxobacteria, 30 specifically inhibited P-body assembly. Gephyronic acid A (GA), a eukaryotic protein synthesis inhibitor, showed the strongest effect. GA also inhibited, under stress conditions, phosphorylation of eIF2 and stress granule formation. Other hits uncovered interesting novel links between P-body assembly, lipid metabolism, and internal organelle physiology. The obtained results provide a chemical toolbox to manipulate P-body assembly and function.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据