期刊
RNA BIOLOGY
卷 9, 期 11, 页码 1305-1310出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/rna.22343
关键词
influenza; pseudoknot; splicing; structure mapping; virus; conformational switch
资金
- NIGMS NIH HHS [T32 GM068411, R01 GM022939] Funding Source: Medline
The 3' splice site of the influenza A segment 7 transcript is utilized to produce mRNA for the critical M2 ion-channel protein. In solution a 63 nt fragment that includes this region can adopt two conformations: a pseudoknot and a hairpin. In each conformation, the splice site, a binding site for the SF2/ASF exonic splicing enhancer and a polypyrimidine tract, each exists in a different structural context. The most dramatic difference occurs for the splice site. In the hairpin the splice site is between two residues that are involved in a 2 by 2 nucleotide internal loop. In the pseudoknot, however, these bases are canonically paired within one of the pseudoknotted helices. The conformational switching observed in this region has implications for the regulation of splicing of the segment 7 mRNA. A measure of stability of the structures also shows interesting trends with respect to host specificity: avian strains tend to be the most stable, followed by swine and then human.
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