期刊
RNA BIOLOGY
卷 8, 期 2, 页码 325-342出版社
TAYLOR & FRANCIS INC
DOI: 10.4161/rna.8.2.13984
关键词
virus HIV-1; RNP affinity chromatography; mass spectrometry; splicing regulation; nucleolin; hnRNP K
资金
- Alsace Region
- ALIX compagny
- Centre National de la Recherche Scientifique (CNRS)
- the french Ministere de la Recherche et de l'Enseignement Superieur
- Universite de Strasbourg (UdS)
- the french Agence Nationale de la Recherche sur le SIDA (ANRS) [05195-06194, 08061-09069]
- the french Agence Nationale pour la Recherche (ANR) [ANR-05-BLAN-0261-03]
- European Community [LSHG-CT-2005-518238]
- the Pole de Recherche Scientifique et Technologique Bioingenierie of Region Lorraine
- Agence Nationale de la Recherche (ANR) [ANR-05-BLAN-0261] Funding Source: Agence Nationale de la Recherche (ANR)
HIV-1 pre-mRNA splicing depends upon 4 donor and 8 acceptor sites, which are used in combination to produce more than 40 different mRNAs. The acceptor site A7 plays an essential role for tat and rev mRNA production. The SLS2-A7 stem-loop structure containing site A7 was also proposed to modulate HIV-1 RNA export by the Rev protein. To further characterize nuclear factors involved in these processes, we purified RNP complexes formed by incubation of SLS2-A7 RNA transcripts in HeLa cell nuclear extracts by affinity chromatography and identified 33 associated proteins by nanoLC-MS/MS. By UV cross-linking, immunoselection and EMSA, we showed that, in addition to the well-known hnRNP A1 inhibitor of site A7, nucleolin, hnRNP H and hnRNP K interact directly with SLS2-A7 RNA. Nucleolin binds to a cluster of successive canonical NRE motifs in SLS2-A7 RNA, which is unique in HIV-1 RNA. Proteins hnRNP A1 and hnRNP K bind synergistically to SLS2-A7 RNA and both have a negative effect on site A7 activity. By the use of a plasmid expressing a truncated version of HIV-1 RNA, we showed a strong effect of the overexpression of hnRNP K in HeLa cells on HIV-1 alternative splicing. As a consequence, production of the Nef protein was strongly reduced. Interestingly also, many proteins identified in our proteomic analysis are known to modulate either the Rev activity or other mechanisms required for HIV-1 multiplication and several of them seem to be recruited by hnRNP K, suggesting that hnRNP K plays an important role for HIV-1 biology.
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