期刊
RNA BIOLOGY
卷 5, 期 4, 页码 189-192出版社
LANDES BIOSCIENCE
DOI: 10.4161/rna.6859
关键词
Dcp2; decapping; P-bodies; mRNA turnover; mRNA decay
资金
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM078360] Funding Source: NIH RePORTER
- NIGMS NIH HHS [R01 GM078360-01A2, R01 GM078360] Funding Source: Medline
mRNA decapping by Dcp2 is a critical step in several major ettkaryotic mRNA decay pathways. Dcp2 forms the catalytic core of a mRNP that is configured for processing diverse substrates by pathway-specific activators. Here we elaborate a model of catalysis by Dcp2 which posits that activity is controlled by a conformational equilibrium between an open, inactive and closed, active form of the enzyme. Structural studies on yeast Dcp2 indicate that the general activator Dcp1 and substrate promote the closed form of the enzyme. Kinetic studies indicate the catalytic step of decapping is rate-limiting and accelerated by Dcp1. We propose that regulation of conformational transitions in Dcp2 during a rate-limiting step after assembly of the decapping mRNP provides a checkpoint for determining if an mRNA is degraded or recycled to translation.
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