Article
Oncology
Ji Young Yoo, Margaret Yeh, Balveen Kaur, Tae Jin Lee
Summary: Aberrant gene and miRNA expression can drive cancer development, and the modulation of these expressions provides a therapeutic opportunity. Recent advances in RNA nanotechnology offer potential for miRNA therapy, but the lack of a delivery platform remains a challenge.
Article
Plant Sciences
Daniel Endale Gebremichael, Zeraye Mehari Haile, Francesca Negrini, Silvia Sabbadini, Luca Capriotti, Bruno Mezzetti, Elena Baraldi
Summary: The application of exogenous RNAi technology in managing plant pathogenic fungi has shown promising results in reducing disease severity, highlighting the great potential of RNAi-mediated control of fungal diseases in crops.
Article
Plant Sciences
Chengcheng Zhong, Neil A. Smith, Daai Zhang, Xiuhong Gou, Ian K. Greaves, Anthony A. Millar, Tom K. Walsh, Weixing Shan, Ming-Bo Wang
Summary: In this study, a highly efficient small RNA target mimicry design based on G-U base-paired hairpin RNA (hpG:U) was presented, which allows for the simultaneous targeting of multiple sRNAs. The hpG:U construct consistently generates high amounts of intact, polyadenylated stem-loop RNA, leading to effective repression of multiple miRNAs and siRNAs. This technology holds great potential for sRNA functional analysis and the management of sRNA-mediated diseases.
Review
Agriculture, Multidisciplinary
Haitham G. Abo-Al-Ela
Summary: RNAi is a natural mechanism used to silence specific genes and has been extensively studied and applied in aquaculture, particularly in shrimp. Despite limitations, RNAi methods show potential in therapeutic agents and studying biological functions.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2021)
Article
Cell Biology
Steven Rodriguez, Asli Sahin, Benjamin R. Schrank, Hawra Al-Lawati, Isabel Costantino, Eric Benz, Darian Fard, Alefiya D. Albers, Luxiang Cao, Alexis C. Gomez, Kyle Evans, Elena Ratti, Merit Cudkowicz, Matthew P. Frosch, Michael Talkowski, Peter K. Sorger, Bradley T. Hyman, Mark W. Albers
Summary: The study discovered the presence of cdsRNA in the brains of ALS-FTD patients, coinciding with TDP-43 inclusions and inducing IFN-I signaling and cell death. Mouse experiments showed that genomically encoded dsRNA triggered IFN-I and death in connected neurons, potentially driving neuroinflammation and neurodegeneration in ALS/FTD patients.
SCIENCE TRANSLATIONAL MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Ki Hwan Park, Jeong-Wook Yang, Joo-Hee Kwon, Hyunju Lee, Yeo Dae Yoon, Byeong Jo Choi, Myeong Youl Lee, Chang Woo Lee, Sang-Bae Han, Jong Soon Kang
Summary: The study demonstrates that dsVDUP1-834 induces the expression of VDUP1 gene through epigenetic changes, resulting in inhibition of cell growth and cell-cycle arrest in lung cancer cells. The targeted induction of VDUP1 by dsVDUP1-834 might be a promising therapeutic strategy for the treatment of lung cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Oncology
Kelin She, Shaoqi Yu, Shushuai He, Wen Wang, Biao Chen
Summary: This study found that circ_0009043 is upregulated in NSCLC cells and tissues, and lower expression of circ_0009043 is associated with poorer overall survival in NSCLC patients. Mechanistically, circ_0009043 acts as a molecular sponge, sequestering miR-148a-3p and promoting the expression of DNAJB4, thereby acting as a tumor suppressor in NSCLC.
BIOMARKER RESEARCH
(2022)
Article
Plant Sciences
Ahmet Bakirbas, Rosario Castro-Rodriguez, Elsbeth L. Walker
Summary: To identify sRNA functioning during iron deficiency stress, RNA from Arabidopsis thaliana phloem exudates was sequenced. Bioanalyzer analysis revealed the presence of abundant small-sized RNA (<200 nt) in phloem, with no typical rRNA bands observed. Sequencing of eight independent phloem RNA samples showed high abundance of tRNA-derived fragments, specifically 5' tRFs and 5' tRNA halves, constituting approximately 46% of all reads. Additionally, a set of miRNAs present in phloem sap was defined, and differentially expressed miRNAs and sRNAs during iron deficiency were identified.
Review
Plant Sciences
Zhi-Fang Zuo, Wenbo He, Jing Li, Beixin Mo, Lin Liu
Summary: sRNAs, including microRNAs (miRNAs) and small interfering RNAs (siRNAs), play critical roles in the response and acclimation of plants to heat stress. Studies have shown their involvement in high-temperature stress responses and their regulatory mechanisms, providing valuable insights for engineering crops with improved thermotolerance. Global investigations using high-throughput sequencing have unveiled the roles of sRNAs in heat stress responses across various plant species.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Reproductive Biology
Chong Tang, Yeming Xie, Mei Guo, Wei Yan
Summary: AASRA is an all-in-one sncRNA annotation pipeline that offers high-speed, simultaneous annotation of all known sncRNA species while distinguishing mature from precursor microRNAs, and identifying novel sncRNA variants in sequencing reads.
BIOLOGY OF REPRODUCTION
(2021)
Article
Multidisciplinary Sciences
Dandan Qian, Ye Cong, Runhao Wang, Quan Chen, Chuangye Yan, Deshun Gong
Summary: This study presents the cryo-electron microscopy structures of human SIDT2, which forms a dimer and acts as a transporter rather than a channel. Additionally, it exhibits ceramidase activity. These findings enhance our understanding of the structure-function relationships in the SID1 family proteins.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Elena S. Martens-Uzunova, Gina D. Kusuma, Stefania Crucitta, Hong Kiat Lim, Crystal Cooper, James E. Riches, Arun Azad, Takahiro Ochiya, Glen M. Boyle, Melissa C. Southey, Marzia Del Re, Rebecca Lim, Grant A. Ramm, Guido W. Jenster, Carolina Soekmadji
Summary: The study found that circulating vesicles with CD9 and PSMA double-positive were increased in patients with advanced metastatic prostate cancer, while CD9 and CD63 double-positive small EVs were significantly higher in patients with localized prostate cancer. Androgen manipulation altered the heterogeneity and size of CD9 positive S-EVs in prostate cancer cells expressing AR, with no impact on the total number and protein cargo of S-EVs; hormone stimulation had strong and specific effects on the small RNA cargo of S-EVs.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Virology
Takuma Izumi, Yuhei Morioka, Syun-ichi Urayama, Daisuke Motooka, Tomokazu Tamura, Takahiro Kawagishi, Yuta Kanai, Takeshi Kobayashi, Chikako Ono, Akinari Morinaga, Takahiro Tomiyama, Norifumi Iseda, Yukiko Kosai, Shoichi Inokuchi, Shota Nakamura, Tomohisa Tanaka, Kohji Moriishi, Hiroaki Kariwa, Tomoharu Yoshizumi, Masaki Mori, Yoshiharu Matsuura, Takasuke Fukuhara
Summary: A new method for efficient detection of RNA viruses in human clinical samples was established, which showed higher viral read ratio compared to conventional methods and correctly identified viral RNA. This new technique will be an effective tool for intracellular RNA virus surveillance in human clinical samples and may be useful for the detection of new RNA viruses associated with diseases.
Article
Chemistry, Multidisciplinary
Jonathan G. Kwok, Zhi Yuan, Paramjit S. Arora
Summary: RNA folds into diverse secondary and tertiary structures, making it difficult to develop ligands that can specifically target it. In this study, the researchers aimed to develop ligands for dsRNA segments by analyzing high-resolution structures of DNA and RNA. They found that the helical motifs in bZIP/bHLH proteins were not ideal for binding dsRNA major grooves. Through reengineering the leucine zipper motifs with synthetic crosslinkers, they successfully designed a synthetic crosslinked helix fork (CHF) as a proteomimetic scaffold for targeting dsRNA.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Oncology
Qunli Xiong, Yaguang Zhang
Summary: Small RNAs are short RNA molecules that play various important roles in cells. Different types of small RNAs, such as miRNA, piRNA, siRNA, and tsRNA, have diverse functions. Current research suggests that small RNAs can be modified, affecting their stability and nuclear export, and these modifications are relevant to cellular processes like biogenesis, cell proliferation, and differentiation. This review focuses on the molecular characteristics and cellular functions of small RNAs and their modifications, as well as techniques for reliable detection. The potential clinical applications of small RNA modifications in the diagnosis and treatment of conditions like cancer are also discussed.
JOURNAL OF HEMATOLOGY & ONCOLOGY
(2023)
Article
Cell & Tissue Engineering
Ji Wang, Vera Huang, Lin Ye, Alicia Barcena, Guiting Lin, Tom F. Lue, Long-Cheng Li
STEM CELLS AND DEVELOPMENT
(2015)
Article
Cell Biology
Victoria Portnoy, Szu Hua Sharon Lin, Kathy H. Li, Alma Burlingame, Zheng-Hui Hu, Hao Li, Long-Cheng Li
Article
Oncology
Changlin Li, Wencong Jiang, Qingting Hu, Long-cheng Li, Liang Dong, Ruibao Chen, Yinghong Zhang, Yuzhe Tang, J. Brantley Thrasher, Chang-Bai Liu, Benyi Li
Correction
Multidisciplinary Sciences
Robert F. Place, Long-Cheng Li, Deepa Pookot, Emily J. Noonan, Rajvir Dahiya
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2018)
Review
Biochemistry & Molecular Biology
Long-Cheng Li
Article
Biochemistry & Molecular Biology
Chenghe Wang, Zhong Chen, Qiangqiang Ge, Junhui Hu, Fan Li, Jia Hu, Hua Xu, Zhangqun Ye, Long-Cheng Li
Article
Cell Biology
Li Jiao, Zhen Deng, Chuanliang Xu, Yongwei Yu, Yun Li, Chun Yang, Junyi Chen, Zhiyong Liu, Gang Huang, Long-Cheng Li, Yinghao Sun
JOURNAL OF CELLULAR PHYSIOLOGY
(2014)
Article
Engineering, Manufacturing
Ming Jiang, Hairong Li, Dong Fang, Lei Liu, Qile Tai, Longcheng Li, Lijie Dong, Haian Xie, Chuanxi Xiong
MATERIALS AND MANUFACTURING PROCESSES
(2014)
Article
Toxicology
Xue-song Wang, Long-cheng Li, Xue Zhang, Jin Gao
Summary: The study found that lipoxin A(4) methyl ester can protect PC12 cells from ketamine-induced neurotoxicity by activating the miR-22/BAG5 signaling pathway. MiR-22 directly targets BAG5 to promote cell viability and suppress cell apoptosis and oxidative stress.
HUMAN & EXPERIMENTAL TOXICOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Yong-Li Zhang, Moorim Kang, Jian-Cheng Wu, Meng-Yao Xie, Ruo-Yan Xue, Qi Tang, Hua Yang, Long-Cheng Li
Summary: This study explored the potential of using small activating RNAs and growth factors to induce the differentiation of human adipose-derived mesenchymal stem cells into hair cell-like cells. The results showed that selected small activating RNAs effectively activated the expression of the ATOH1 gene and induced the differentiation of hair cell progenitor cells into hair cell-like cells. This RNA activation technology could offer a new strategy for hair cell regeneration.
Article
Chemistry, Physical
Renhui Zhang, Qiao Zhang, Yuzhou Luo, Ruolin Wu, Longcheng Li
Summary: This study systematically investigated the tribological performance of a mixed lubricant containing phenol and benzyl alcohol on YG8 self-mated interfaces. The results showed that the concentration of phenol had a complex effect on the friction coefficient and anti-wear performance. TEM, SEM, and XPS techniques were used to probe the tribological mechanism of the mixed lubricant containing 1.0 wt.% phenol.
SURFACE AND INTERFACE ANALYSIS
(2023)
Article
Multidisciplinary Sciences
Qi Zhang, Yangchen Guo, Moorim Kang, Wei-Hsiang Lin, Jian-Cheng Wu, Ying Yu, Long-Cheng Li, Aimin Sang
Summary: The study suggests that p21 saRNA can be a potential therapeutic approach for PVR by inhibiting cell proliferation and migration of RPE cells, leading to the prevention of PVR progression and reducing postoperative failure rates.
Article
Medicine, General & Internal
Longcheng Li, Yang Xu, Fei Jiang, Xue Zhang
Summary: Comparing the effects of TAPB and QLB on postoperative recovery in patients undergoing laparoscopic radical resection for rectal cancer, the study found that the QLB group had higher QoR-15 scores, lower sufentanil consumption, longer time to first postoperative PCA compression, and fewer compression occurrences.
IRANIAN RED CRESCENT MEDICAL JOURNAL
(2022)
Article
Engineering, Biomedical
Xun Jin, Jingwei Ma, Xiaoyu Liang, Ke Tang, Yuying Liu, Xiaonan Yin, Yi Zhang, Huafeng Zhang, Pingwei Xu, Degao Chen, Tianzhen Zhang, Jinzhi Lu, Zhuowei Hu, Xiaofeng Qin, Xiaoyong Zeng, Longcheng Li, Bo Huang
Proceedings Paper
Engineering, Electrical & Electronic
Long-Cheng Li, Sheng-Qiang Zhou
PROCEEDINGS OF 2016 SYMPOSIUM ON PIEZOELECTRICITY, ACOUSTIC WAVES, AND DEVICE APPLICATIONS (SPAWDA)
(2016)
