期刊
RHEUMATOLOGY INTERNATIONAL
卷 33, 期 1, 页码 79-83出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s00296-011-2339-3
关键词
Osteoarthritis; Fibroblast-like synoviocytes; Osteopontin; Hyaluronic acid; CD44
类别
资金
- National 863 project of China [2011AA030101]
- National Natural Science Foundation of China [30300396]
- Provincial Science Foundation of Hunan [09JJ3048]
- Graduate degree thesis Innovation Foundation of Hunan Province [CX-2010B102]
- National Clinical Key Department Construction Projects of China
The aim of this study was to investigate the effect of hyaluronic acid (HA) on the expression of osteopontin (OPN) and CD44 mRNA of fibroblast-like synoviocytes (FLS) in the patients with osteoarthritis (OA) of the knee. FLS were obtained from the knees of 10 patients with OA. Real-time quantitative polymerase chain reaction (Q-PCR) was used to assess the expression of the OPN mRNA and CD44 mRNA. The relative OPN mRNA expression in HA group (6.47 +/- A 2.30-fold) was significantly higher than in the control group (P = 0.045, P < 0.05), while in HYL group (0.65 +/- A 0.21-fold) it was lower than in the control group (P = 0.037, P < 0.05), and the difference in OPN mRNA expression between HA group and HYL group also showed statistically significant (P = 0.001, P < 0.05); however, there was no significant difference between each group of the relative CD44 mRNA expression (P > 0.05). Our study showed that HA can upregulate OPN mRNA expression in OA fibroblast-like synoviocytes, and the high expression of OPN mRNA in OA may be a result of increased HA level of OA synovitis; however, HA cannot affect the CD44 mRNA expression in OA fibroblast-like synoviocytes, and the high expression of CD44 mRNA in OA may be not a result of increased HA level of OA synovitis.
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