期刊
ONCOTARGET
卷 6, 期 33, 页码 34968-34978出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.5198
关键词
microRNA; miR-200c; KRAS; breast cancer; proliferation
资金
- National Natural Science Foundation of China [81472575, 81472469, 81272514, 81402183]
- Key Programme of the National Natural Science Foundation of China [31030061]
- China Postdoctoral Science Foundation [2012M520075, 2014M550447]
- Science and Technology Planning Project of Guangzhou and Guangdong [2014J4100169, 2013B060300009]
The microRNA, miR-200c, is involved in the tumorigenesis and progression of a variety of cancers. The purpose of this study was to investigate the expression, mechanism and prognostic roles of miR-200c in breast cancer. We found that miR-200c was downregulated in both breast cancer tissue and cell lines using quantitative real-time PCR (qRT-PCR). In situ hybridization (ISH) and microarrays showed that low miR-200c expression was associated with poor patient overall survival (OS) and disease free survival (DFS). We used luciferase reporter plasmids to find that miR-200c inhibited the AKT and ERK pathways by directly targeting KRAS. Repression of KRAS by miR-200c suppressed the proliferation and survival of breast cancer cells in vitro and in vivo. miR-200c also had an anti-tumor effect by negatively regulating KRAS in a xenograft mouse model. Our findings provide clues regarding the role of miR-200c as a tumor suppressor in breast cancer through the inhibition of KRAS translation both in vitro and in vivo. miR-200c could be a potential therapeutic target in breast cancer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据