Article
Medicine, General & Internal
Keiichiro Yoshioka, Hironori Sato, Takeshi Kawasaki, Daisuke Ishii, Takuro Imamoto, Mitsuhiro Abe, Yoshinori Hasegawa, Osamu Ohara, Koichiro Tatsumi, Takuji Suzuki
Summary: RNA sequencing analysis revealed differential gene expression patterns in peripheral blood mononuclear cells (PBMCs) between patients with pulmonary sarcoidosis and healthy controls, with 270 differentially expressed genes involved in various biological processes related to the pathobiology of sarcoidosis. Enrichment analysis of gene ontology and KEGG pathways indicated the potential involvement of specific signaling pathways in sarcoidosis pathogenesis, providing insight into potential therapeutic targets for the disease.
FRONTIERS IN MEDICINE
(2022)
Article
Immunology
Yun Li, Haixiang Zhou, Qian Huang, Wei Tan, Yuting Cai, Zicong Wang, Jingling Zou, Bingyan Li, Shigeo Yoshida, Yedi Zhou
Summary: This study reveals the altered expression profiles of circular RNAs in the peripheral blood mononuclear cells of patients with retinopathy of prematurity and identifies potential biomarkers for ROP diagnosis. The findings suggest that circRNAs could be used as diagnostic biomarkers and therapeutic targets for ROP.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Zhuolin Wang, Yang Zhang, Rirong Yang, Yujia Wang, Jiapei Guo, Ruya Sun, Yuan Zhou, Li Su, Qing Ge, Yingmei Feng
Summary: The immune response plays a critical role in the progression of pulmonary fibrosis (PF) in COVID-19 patients. This study found that CD4+ activated T cells and Treg cells were more frequent in patients with a higher PF index (FIhi), indicating enhanced immune cell activity. Gene expression analysis also revealed the downregulation of several key genes, which may be associated with the development of pulmonary fibrosis.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Jing Wei, Fangzheng Guo, Yamin Song, Kun Xu, Feiyang Lin, Kangsheng Li, Baiqing Li, Zhongqing Qian, Xiaojing Wang, Hongtao Wang, Tao Xu
Summary: In this study, RNA sequencing of Mtb-Ag-stimulated and control samples identified differentially expressed genes related to immune function. These genes are primarily involved in cellular pathways associated with immune function, revealing the immune response mechanism triggered by Mtb-Ag stimulation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Genetics & Heredity
Fei Dai, Quan-Bo Zhang, Yi-Ping Tang, Yi-Xi He, Ting Yi, Yu-Feng Qing
Summary: Circular RNAs (circRNAs) with abnormal expression levels were identified in the peripheral blood mononuclear cells (PBMCs) of patients with primary gout. These differentially expressed circRNAs are likely to play crucial roles in the development and progression of gout.
FRONTIERS IN GENETICS
(2021)
Article
Medicine, General & Internal
Li Xue, Biao Wang, Jianhong Zhu, Qian He, Fang Huang, Wei Wang, Li Tao, Yan Wang, Nan Xu, Ni Yang, Li Jin, Hua Zhang, Ning Gao, Ke Lei, Yanping Zhang, Chaoliang Xiong, Jing Lei, Ting Zhang, Yan Geng, Ming Li
Summary: This study aimed to investigate the key circRNAs related to rheumatoid arthritis (RA) by identifying differentially expressed circRNAs (DEcircRNAs) in peripheral blood mononuclear cells (PBMCs) from RA patients compared to controls and osteoarthritis (OA) patients. Real-time PCR validation and correlation analysis with laboratory indices were also performed. The functional annotation of host genes of the DEcircRNAs in RA revealed their involvement in metabolic pathways, ECM-receptor interaction, and the PI3K-Akt signalling pathway. These findings contribute to a better understanding of the role of circRNAs in the specific mechanism underlying RA.
ANNALS OF MEDICINE
(2023)
Article
Immunology
Lijing Wang, Hongjun Zhao, Indu Raman, Mei Yan, Qiong Chen, Quan-Zhen Li
Summary: The mechanisms underlying chronic obstructive pulmonary disease (COPD) remain unclear. This study identified differentially expressed genes and miRNAs in peripheral blood mononuclear cells (PBMCs) of COPD patients, compared with normal controls. The results suggest that miRNAs and mRNAs are involved in the pathogenesis of COPD.
JOURNAL OF INFLAMMATION RESEARCH
(2022)
Article
Medicine, General & Internal
Natascha Sommer, Finn Fabian Theine, Oleg Pak, Khodr Tello, Manuel Richter, Henning Gall, Jochen Wilhelm, Rajkumar Savai, Norbert Weissmann, Werner Seeger, Hossein A. Ghofrani, Matthias Hecker
Summary: The study found that mitochondrial function in PBMC of PAH patients is affected by disease severity, with mitochondrial respiration correlating with hemodynamic parameters. Further research is needed to investigate cell-type-specific alterations and functional consequences.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
G. F. Bouvet, O. Bulka, A. Coriati, L. Sognigbe, G. St-Pierre, C. Masse, S. Sato, Y. Berthiaume
Summary: The study demonstrated variable impacts of YKL-40 on IL-6, IL-10, and IL-13 gene expression in CF PBMCs, as well as an interaction between GAL3 and YKL-40.
Article
Immunology
Satoshi Gondaira, Koji Nishi, Hidetomo Iwano, Jumpei Fujiki, Reina Watanabe, Ayako Eguchi, Yuki Hirano, Hidetoshi Higuchi, Hajime Nagahata
Summary: The study revealed an increase in the transcriptional abundance of innate immunity genes in PBMCs during Mycoplasma bovis infection, leading to the activation of PBMCs and subsequent immune response and inflammation. These findings could be essential for the development of novel vaccine candidates against M. bovis.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2021)
Article
Immunology
Anderson Rodrigues de Almeida, Andrea Tavares Dantas, Maria Eduarda de Oliveira Goncalves, Charlotte Chene, Mohamed Jeljeli, Sandrine Chouzenoux, Marine Thomas, Eudes Gustavo Constantino Cunha, Lilian David de Azevedo Valadares, Joao Victor de Melo Gomes, Simao Kalebe Silva de Paula, Marina Galdino da Rocha Pitta, Ivan da Rocha Pitta, Moacyr Jesus Barreto de Melo Rego, Michelly Cristiny Pereira, Angela Luzia Branco Pinto Duarte, Dulcineia Saes Parra Abdalla, Carole Nicco, Frederic Batteux, Maira Galdino da Rocha Pitta
Summary: This study aimed to investigate the immunomodulatory and antifibrotic properties of a TZD derivative called LPSF/GQ-16 in peripheral blood mononuclear cells (PBMC) from SSc patients and in a murine model of SSc. The results showed that LPSF/GQ-16 modulated the secretion of cytokines and significantly reduced dermal fibrosis in the mouse model. This study suggests the therapeutic potential of LPSF/GQ-16 for SSc.
INFLAMMOPHARMACOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Byeongyeon Kim, In-Ho Seo, Gwang Myeong Seo, Woo-Joong Kim, Eui-Cheol Shin, Sungyoung Choi
Summary: The study introduces a blood divider (B-DV) as a centrifuge tube insert to prevent RBC contamination during PBMC harvest, simplifying the separation process and enabling rapid, easy PBMC isolation.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Article
Pharmacology & Pharmacy
Vera Vorstandlechner, Dragan Copic, Katharina Klas, Martin Direder, Bahar Golabi, Christine Radtke, Hendrik J. Ankersmit, Michael Mildner
Summary: This study demonstrates the anti-fibrotic effect of PBMCsec on cutaneous scars in both mouse and human experimental settings. PBMCsec regulates the expression of fibrosis-related genes, attenuates myofibroblast differentiation, and inhibits the breakdown of elastic fibers. These findings suggest that PBMCsec could be a novel therapeutic option for treating skin scarring.
Article
Neurosciences
Yuqing Wang, Heng Zhao, Xiaoqiang Liu, Lei Yan
Summary: The authors discovered altered expression of lncRNAs in PBMCs of AD patients using microarray, suggesting the need for a specialized high-level microarray analysis method to obtain reliable results and further clarify the data analysis strategy of the study.
MOLECULAR NEUROBIOLOGY
(2021)
Article
Medicine, General & Internal
Hong-Miao Li, Qian Huang, Fen Tang, Tian-Ping Zhang
Summary: The study found that NCF2 and NOX2 mRNA levels were significantly increased in PBMC of PTB patients, but these levels were not associated with major clinical and laboratory data of PTB patients. NCF2 and NOX2 have potential diagnostic value in PTB patients.
INTERNATIONAL JOURNAL OF GENERAL MEDICINE
(2021)
Article
Rheumatology
Kuniyuki Aso, Michihito Kono, Nobuya Abe, Yuichiro Fujieda, Masaru Kato, Tatsuya Atsumi
Summary: Anti-gAChR alpha 3 antibodies could be a predictive biomarker for the development or relapse of lupus enteritis in patients with systemic lupus erythematosus.
MODERN RHEUMATOLOGY
(2023)
Article
Rheumatology
Hiroto Tsuboi, Hirofumi Toko, Fumika Honda, Saori Abe, Hiroyuki Takahashi, Mizuki Yagishita, Shinya Hagiwara, Ayako Ohyama, Yuya Kondo, Kazuhisa Nakano, Yoshiya Tanaka, Toshimasa Shimizu, Hideki Nakamura, Atsushi Kawakami, Yuichiro Fujieda, Tatsuya Atsumi, Yasunori Suzuki, Mitsuhiro Kawano, Naoshi Nishina, Yuko Kaneko, Tsutomu Takeuchi, Hitomi Kobayashi, Masami Takei, Michihiro Ogasawara, Naoto Tamura, Yoshinari Takasaki, Kazuhiro Yokota, Yuji Akiyama, Toshihide Mimura, Kosaku Murakami, Tsuneyo Mimori, Shiro Ohshima, Naoto Azuma, Hajime Sano, Susumu Nishiyama, Isao Matsumoto, Takayuki Sumida
Summary: This study found that intravenous abatacept is effective in treating Sjogren's syndrome associated with rheumatoid arthritis. It improves both glandular and extraglandular involvements, as well as systemic disease activities and patient-reported outcomes. Adverse events were low, and adherence to abatacept was high.
MODERN RHEUMATOLOGY
(2023)
Article
Rheumatology
Hiromu Ito, Keiichiro Nishida, Toshihisa Kojima, Isao Matsushita, Masayo Kojima, Shintaro Hirata, Yuko Kaneko, Mitsumasa Kishimoto, Masataka Kohno, Masaaki Mori, Akio Morinobu, Atsuko Murashima, Yohei Seto, Takahiko Sugihara, Eiichi Tanaka, Takeo Nakayama, Hisashi Yamanaka, Yutaka Kawahito, Masayoshi Harigai
Summary: This study aimed to update the clinical practice guidelines for the management of rheumatoid arthritis (RA) in Japan and develop an algorithm for non-drug and surgical treatments. The guidelines were updated using the Grading of Recommendations, Assessment, Development, and Evaluation method, and recommendations were developed based on 19 clinical questions regarding non-drug and surgical treatments. The study also created an original algorithm for the treatment of RA.
MODERN RHEUMATOLOGY
(2023)
Article
Rheumatology
Eiichi Tanaka, Eisuke Inoue, Mai Abe, Kumiko Saka, Eri Sugano, Moeko Ochiai, Rei Yamaguchi, Katsunori Ikari, Hisashi Yamanaka, Masayoshi Harigai
MODERN RHEUMATOLOGY
(2022)
Article
Rheumatology
Yutaka Kawahito, Akio Morinobu, Yuko Kaneko, Masataka Kohno, Shintaro Hirata, Mitsumasa Kishimoto, Yohei Seto, Takahiko Sugihara, Eiichi Tanaka, Hiromu Ito, Toshihisa Kojima, Isao Matsushita, Keiichiro Nishida, Masaaki Mori, Atsuko Murashima, Hisashi Yamanaka, Takeo Nakayama, Masayo Kojima, Masayoshi Harigai
Summary: The Japan College of Rheumatology has updated the clinical practice guidelines for rheumatoid arthritis, providing a useful tool for rheumatologists, healthcare professionals, and patients to make shared decisions in various clinical situations.
MODERN RHEUMATOLOGY
(2023)
Article
Rheumatology
Hisashi Yamanaka, Yoshiya Tanaka, Toshihiko Hibino, Gopalakrishnan Unmesh, Chirag Shah, Dhananjay Bakhle, Dimitris Stefanidis
Summary: YLB113 biosimilar demonstrated long-term safety and sustained efficacy in patients with rheumatoid arthritis. In a post-hoc analysis, patients treated with YLB113 experienced significantly lower incidence of injection-site reactions and injection-site erythema compared with the reference product.
INTERNATIONAL JOURNAL OF RHEUMATIC DISEASES
(2023)
Article
Rheumatology
Keita Ninagawa, Masaru Kato, Yasuka Kikuchi, Hiroyuki Sugimori, Michihito Kono, Yuichiro Fujieda, Ichizo Tsujino, Tatsuya Atsumi
Summary: This study aimed to predict the response to pulmonary vasodilators in patients with SSc and PH. The results showed that quantitative chest computed tomography can well predict the response to vasodilators, suggesting its utility in differentiating the dominance of pulmonary vascular disease or interstitial lung disease.
MODERN RHEUMATOLOGY
(2023)
Article
Rheumatology
Kohei Karino, Michihito Kono, Shuhei Takeyama, Yuki Kudo, Masatoshi Kanda, Nobuya Abe, Kuniyuki Aso, Yuichiro Fujieda, Masaru Kato, Kenji Oku, Olga Amengual, Tatsuya Atsumi
Summary: This study identified Ikbke as a potential therapeutic target for NPSLE based on its involvement in microglial activation and phagocytosis. Inhibition of IKBK epsilon improved cognitive function and suppressed microglial activation in MRL/lpr mice, suggesting its importance in the pathogenesis of NPSLE.
ARTHRITIS & RHEUMATOLOGY
(2023)
Editorial Material
Rheumatology
Kuniyuki Aso, Yuichiro Fujieda, Tatsuya Atsumi
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Editorial Material
Medicine, General & Internal
Kuniyuki Aso, Yuichiro Fujieda, Tatsuya Atsumi
Article
Rheumatology
Keita Ninagawa, Masaru Kato, Satonori Tsuneta, Suguru Ishizaka, Hideyuki Ujiie, Ryo Hisada, Michihito Kono, Yuichiro Fujieda, Yoichi M. Ito, Tatsuya Atsumi
Summary: Nintedanib has beneficial effects on cardiomyopathy associated with SSc, suggesting its anti-fibrotic effect may not be limited to the lungs.
Article
Rheumatology
Claudia A. Salinas, Anthony Louder, Jennifer Polinski, Tancy C. Zhang, Hannah Bower, Syd Phillips, Yufei Song, Emaan Rashidi, Rafia Bosan, Hsiu-Ching Chang, Nicole Foster, Bernice Gershenson, Hisashi Yamanaka, Mitsumasa Kishimoto, Yoshiya Tanaka, Peter Fischer, Baojin Zhu, Douglas Faries, Xiaodan Mai, Brett T. Doherty, Angela Grelaud, Nicolas H. Thurin, Johan Askling, Walter Deberdt
Summary: This study aimed to evaluate the safety of baricitinib compared to tumor necrosis factor inhibitors (TNFi) in rheumatoid arthritis (RA) patients. The results showed an increased risk of venous thromboembolism (VTE) with baricitinib compared to TNFi treatment. Although the risk was slightly higher for major adverse cardiovascular events (MACE) and serious infection, the point estimates varied.
RHEUMATOLOGY AND THERAPY
(2023)
Article
Neurosciences
Kodai Sakiyama, Nobuya Abe, Yuichiro Fujieda, Khin K. Tha, Hisashi Narita, Kohei Karino, Masatoshi Kanda, Michihito Kono, Masaru Kato, Tatsuya Atsumi
Summary: Aberrant functional connectivity (FC) of the brain regions, evaluated by functional magnetic resonance imaging (fMRI), affects clinical courses in inflammatory arthritis (IA). The dynamic FC and corticocortical connectivity play a role in therapeutic responsiveness to biologics in IA patients. The frequent emergence of corticocortical connections is associated with clinical outcomes in IA.
Letter
Rheumatology
H. Moriya, Y. Fujieda, O. Amengual, T. Kanbayashi, T. Atsumi
SCANDINAVIAN JOURNAL OF RHEUMATOLOGY
(2023)
Article
Multidisciplinary Sciences
Kuniyuki Aso, Michihito Kono, Masatoshi Kanda, Yuki Kudo, Kodai Sakiyama, Ryo Hisada, Kohei Karino, Yusho Ueda, Daigo Nakazawa, Yuichiro Fujieda, Masaru Kato, Olga Amengual, Tatsuya Atsumi
Summary: The study reveals that itaconate can alter T cell function and impact the development of autoimmune diseases through modulation of metabolic and epigenetic programs. Itaconate inhibits Th17 cell differentiation and promotes Treg cell differentiation by suppressing glycolysis and oxidative phosphorylation. The metabolic changes induced by itaconate also affect chromatin accessibility, transcription factor binding, and gene expression in Th17 and Treg cell differentiation. Furthermore, the adoptive transfer of itaconate-treated Th17 cells can ameliorate autoimmune encephalomyelitis. These findings suggest that itaconate is a crucial metabolic regulator for Th17/Treg cell balance and can be a potential therapeutic agent for autoimmune diseases.
NATURE COMMUNICATIONS
(2023)