Article
Oncology
Qiaoling Xiao, Can Lin, Meixi Peng, Jun Ren, Yipei Jing, Li Lei, Yonghong Tao, Junpeng Huang, Jing Yang, Minghui Sun, Jing Wu, Zailin Yang, Zesong Yang, Ling Zhang
Summary: The study found that some lncRNAs in plasma exosomes of acute myeloid leukemia (AML) patients were upregulated or downregulated compared to healthy donors. These lncRNAs have the potential to serve as biomarkers for AML diagnosis and treatment monitoring, providing a new possibility for liquid biopsy of this type of cancer.
FRONTIERS IN ONCOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Vijendra Singh, Mohammed Hafiz Uddin, Jeffrey A. Zonder, Asfar S. Azmi, Suresh Kumar Balasubramanian
Summary: While mechanistic studies have shed light on the molecular mechanisms of AML and led to the development of new targeted therapies, resistance remains a significant issue in AML treatment. The exploration of circRNA in AML biology and therapy resistance shows promise in providing new insights and potential solutions to managing this disease.
Article
Oncology
Jolien Vanhooren, Laurens Van Camp, Barbara Depreter, Martijn de Jong, Anne Uyttebroeck, An Van Damme, Laurence Dedeken, Marie-Francoise Dresse, Jutte van der Werff ten Bosch, Mattias Hofmans, Jan Philippe, Barbara De Moerloose, Tim Lammens
Summary: Survival rates for children with acute myeloid leukemia have significantly improved, but a considerable proportion of patients still face the risk of treatment failure or relapse. This study investigates the expression of non-coding RNA in pediatric AML, providing a foundation for further translational research.
Article
Biotechnology & Applied Microbiology
Jingyi Han, Qinqin Liu, Yao Zhou, Dong Li, Ran Wang
Summary: This study revealed significant differences in the levels and patterns of m7G methylation between drug-resistant AML cells and AML cells. Furthermore, the study predicted the cellular functions potentially influenced by m7G in drug-resistant AML cells, providing evidence implicating m7G-mediated lncRNA epigenetic regulation in the progression of drug resistance in AML.
Article
Pharmacology & Pharmacy
Patrick Connerty, Ernest Moles, Charles E. de Bock, Nisitha Jayatilleke, Jenny L. Smith, Soheil Meshinchi, Chelsea Mayoh, Maria Kavallaris, Richard B. Lock
Summary: The study developed a new lipid nanoparticle loaded with siRNA for silencing oncogenic lncRNA LINC01257, demonstrating its potential feasibility in the treatment of AML.
Article
Genetics & Heredity
Zhiyuan Zheng, Wei Wu, Zehang Lin, Shuhan Liu, Qiaoqian Chen, Xiandong Jiang, Yan Xue, Donghong Lin
Summary: This study identified seven novel ferroptosis-related lncRNA signatures in AML, which were shown to accurately predict the prognosis of AML as independent prognostic predictors. These signatures provide new insights into strategies for the development of new AML therapies.
BMC MEDICAL GENOMICS
(2021)
Article
Medicine, General & Internal
Hongjuan Xue, Haili Gao, Hong Xia, Shaofei Li, Na Li, Yuwen Duan, Yanfei Ren, Henglu Zhang, Jingzheng Liu, Wei Gao
Summary: This study demonstrated that Lnc-MEG3 expression was reduced in pediatric AML patients and correlated with specific subtypes and risk stratification, showing an up-regulation trend after treatment. Increased Lnc-MEG3 expression before treatment was associated with longer event-free survival, while enhanced expression after treatment was correlated with both prolonged event-free and overall survival. Lnc-MEG3 may have clinical significance as a biomarker for disease management, treatment optimization, and prognosis improvement in pediatric AML patients.
Review
Oncology
Long Su, Yuan-Yuan Shi, Zeng-Yan Liu, Su-Jun Gao
Summary: This review summarizes the research progress and treatment methods of CEBPA gene mutations in AML, proposes a new treatment algorithm, and may be beneficial for further investigation and optimizing clinical management of AML patients with CEBPA mutations.
FRONTIERS IN ONCOLOGY
(2022)
Article
Cell Biology
Patrick Connerty, Richard B. Lock
Summary: Long noncoding RNAs (lncRNAs), defined as RNA transcripts longer than 200 nucleotides with no protein coding potential, have been found to be dysregulated in various cancers, including acute myeloid leukemia (AML). As survival rates for AML have plateaued, the identification of lncRNAs as novel biomarkers and therapeutic targets for AML is crucial. This review assesses key studies that have identified lncRNAs in AML and summarizes the current knowledge of their roles, including driving proliferation, differentiation block, therapy resistance, tumor suppression, and utility as biomarkers.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2023)
Review
Biochemistry & Molecular Biology
Princess D. Rodriguez, Hana Paculova, Sophie Kogut, Jessica Heath, Hilde Schjerven, Seth Frietze
Summary: Studies have shown that non-coding RNAs (ncRNAs) play central roles in the pathogenesis of B-cell acute lymphoblastic leukemia (B-ALL), providing new insights into the molecular mechanisms of the disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Xiong-yu Liao, Jian-pei Fang, Dun-hua Zhou, Kun-yin Qiu
Summary: CEBPA mutations are associated with a better prognosis in pediatric AML patients, even in the presence of FLT3/ITD mutations. Stem cell transplantation may not be necessary for pediatric AML patients with coexistence of CEBPA and FLT3/ITD mutations.
HEMATOLOGICAL ONCOLOGY
(2022)
Article
Oncology
Zhenqing Tan, Kaibo Zhu, Yafei Yin, Zimian Luo
Summary: This study revealed that lncRNA ANRIL is significantly increased in AML patients and associated with clinical features and prognosis. High expression of lncRNA ANRIL is correlated with low CR rate and shorter EFS and OS in AML patients.
MOLECULAR MEDICINE REPORTS
(2021)
Review
Oncology
Mohammed Awal Issah, Dansen Wu, Feng Zhang, Weili Zheng, Yanquan Liu, Haiying Fu, Huarong Zhou, Rong Chen, Jianzhen Shen
Summary: Canonical epigenetic modifications, including histone modification, chromatin remodeling, and DNA methylation, play key roles in numerous cellular processes. Recent studies have shown an increase in research on epigenetic modifications of RNA, such as circRNAs, which may have important functions in diseases like acute myeloid leukemia. The epigenetic modification of circRNAs, particularly m(6)A, may become a potential diagnostic and therapeutic target for AML in the future.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2021)
Review
Oncology
Yuanyuan Nie, Long Su, Wei Li, Sujun Gao
Summary: Acute myeloid leukemia with bi-allelic CEBPA mutation was previously considered a distinct disease with a good prognosis, but recent research has shown significant heterogeneity within this group, emphasizing the need for further risk stratification. Factors such as non-classical CEBPA mutations, companion genetic aberrations, and germline CEBPA mutations contribute to this heterogeneity. Identifying these factors can help predict relapse risk and guide treatment decisions.
CRITICAL REVIEWS IN ONCOLOGY HEMATOLOGY
(2021)
Article
Pathology
Changhao Han, Yan Qi, Yuanting She, Meijuan Zhang, Huan Xie, Jing Zhang, Zhongyue Zhao, Cuicui Peng, Yu Liu, Yizhang Lin, Jin Wang, Dongfeng Zeng
Summary: Research shows that the aberrant expression of SENCR in acute myeloid leukemia (AML) plays an important role in regulating the malignant phenotype of AML cells through the miR-4731-5p/IRF2 axis.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Correction
Oncology
Ilaria Martinelli, Chiara Modica, Cristina Chiriaco, Cristina Basilico, James M. Hughes, Simona Corso, Silvia Giordano, Paolo M. Comoglio, Elisa Vigna
JOURNAL OF EXPERIMENTAL & CLINICAL CANCER RESEARCH
(2022)
Article
Biochemical Research Methods
Anne F. Nielsen, Albrecht Bindereif, Irene Bozzoni, Mor Hanan, Thomas B. Hansen, Manuel Irimia, Sebastian Kadener, Lasse S. Kristensen, Ivano Legnini, Mariangela Morlando, Morten T. Jarlstad Olesen, R. Jeroen Pasterkamp, Stephan Preibisch, Nikolaus Rajewsky, Christin Suenkel, Jorgen Kjems
Summary: There is a growing number of circular RNA (circRNA) papers in recent years, highlighting the urgent need for common experimental standards and best practice principles to advance the field and enhance understanding of this group of RNA molecules.
Article
Biochemistry & Molecular Biology
Paolo Rosa, Sofia Scibetta, Giuseppe Pepe, Giorgio Mangino, Luca Capocci, Sam J. Moons, Thomas J. Boltje, Francesco Fazi, Vincenzo Petrozza, Alba Di Pardo, Vittorio Maglione, Antonella Calogero
Summary: This study investigated the effect of PSA inhibition on GBM cells under low oxygen concentrations and found that it could inhibit GBM cell migration and differentiation. This finding suggests that PSA may be a potential target for developing therapies for GBM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Guillermo Fernandez Rodriguez, Bianca Cesaro, Alessandro Fatica
Summary: Despite its discovery in the early 1970s, the importance of m(6)A modification in regulating mRNA translation has only recently gained attention in oncology. This chemical modification can control all aspects of mRNA maturation and its alteration may contribute to gene expression changes observed in cancer. In this review, the role of m(6)A in translation regulation and its cancer-related functions are highlighted.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Bianca Cesaro, Marco Tarullo, Alessandro Fatica
Summary: The field of RNA modification, known as epitranscriptomics, is attracting more attention from scientists. Over 160 chemical modifications have been found in RNA molecules, but their functions are still unclear. This review focuses on the role of N-6,2'-O-dimethyladenosine (m(6)A(m)) in gene expression regulation. While the role of m(6)A(m) in splicing regulation has been supported by studies, conflicting data exist regarding its role in mRNA stability and translation. Nonetheless, promising results have been obtained by modulating regulators in cancer cells. Further investigation on m(6)A(m) is expected to yield significant findings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Editorial Material
Cell Biology
Ling-Ling Chen, Albrecht Bindereif, Irene Bozzoni, Howard Y. Y. Chang, A. Gregory Matera, Myriam Gorospe, Thomas B. B. Hansen, Jorgen Kjems, Xu-Kai Ma, Jun Wei Pek, Nikolaus Rajewsky, Julia Salzman, Jeremy E. E. Wilusz, Li Yang, Fangqing Zhao
Summary: This Comment calls for a common nomenclature for naming circRNAs to ensure clarity and reproducibility in studies.
NATURE CELL BIOLOGY
(2023)
Article
Multidisciplinary Sciences
Grygoriy Zolotarov, Bastian Fromm, Ivano Legnini, Salah Ayoub, Gianluca Polese, Valeria Maselli, Peter J. Chabot, Jakob Vinther, Ruth Styfhals, Eve Seuntjens, Anna Di Cosmo, Kevin J. Peterson, Nikolaus Rajewsky
Summary: Research suggests that RNA regulation, particularly the expansion of the miRNA gene repertoire, plays a crucial role in the cognitive success and evolution of complex animal brains, specifically in cephalopods and vertebrates.
Editorial Material
Biochemistry & Molecular Biology
Alessandro Fatica
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Dario Dattilo, Gaia Di Timoteo, Adriano Setti, Andrea Giuliani, Giovanna Peruzzi, Manuel Beltran Nebot, Alvaro Centron-Broco, Davide Mariani, Chiara Mozzetta, Irene Bozzoni
Summary: The study identifies m(6)A machinery and the RNA helicase DDX5 as factors responsible for the increase of a subset of circRNAs in RMS, providing protein and RNA candidates for the study of its tumorigenicity.
NATURE COMMUNICATIONS
(2023)
Article
Microbiology
Agnieszka Rybak-Wolf, Emanuel Wyler, Tancredi Massimo Pentimalli, Ivano Legnini, Anna Oliveras Martinez, Petar Glazar, Anna Loewa, Seung Joon Kim, Benedikt B. Kaufer, Andrew Woehler, Markus Landthaler, Nikolaus Rajewsky
Summary: Modeling herpes simplex virus infection in brain organoids revealed that combined antiviral and anti-inflammatory treatment can prevent neuronal damage caused by viral infection. Despite antiviral treatment, herpes simplex encephalitis often results in neurological sequelae. The study used various techniques to characterize HSV-1 infection in human brain organoids and identified potential causal factors in neuronal damage and tissue perturbations. Combining anti-inflammatory drugs with antiviral treatment showed promise in preventing infection-related damages and improving therapeutic strategies.
NATURE MICROBIOLOGY
(2023)
Article
Biochemical Research Methods
Ivano Legnini, Lisa Emmenegger, Alessandra Zappulo, Agnieszka Rybak-Wolf, Ricardo Wurmus, Anna Oliveras Martinez, Cledi Cerda Jara, Anastasiya Boltengagen, Tale Hessler, Guido Mastrobuoni, Stefan Kempa, Robert Zinzen, Andrew Woehler, Nikolaus Rajewsky
Summary: This study combines optogenetics and gene perturbation technologies to activate or knock down target genes in organoids with programmable spatiotemporal patterns. The research shows that local activation of specific signaling pathways is sufficient to generate organoids with stereotypical patterns and provides new insights into gene regulation in neurodevelopment. Thus, optogenetic perturbations in combination with spatial transcriptomics is a powerful technology for reprogramming and studying cell fates and tissue patterning in organoids.
Article
Biochemistry & Molecular Biology
Alvaro Centron-Broco, Francesca Rossi, Chiara Grelloni, Raffaele Garraffo, Dario Dattilo, Andrea Giuliani, Gaia Di Timoteo, Alessio Colantoni, Irene Bozzoni, Manuel Beltran Nebot
Summary: In this study, a circular RNA called circAFF1 was found to be overexpressed in alveolar rhabdomyosarcoma. Depletion of circAFF1 was shown to affect cell homeostasis and promote cell migration through the downregulation of genes involved in cell adhesion pathways. These findings highlight the potential of circAFF1 as a future therapeutic target for alveolar rhabdomyosarcoma.
Review
Biochemistry & Molecular Biology
Francesca Liccardo, Alessia Iaiza, Martyna Sniegocka, Silvia Masciarelli, Francesco Fazi
Summary: Remodeling of the bone marrow niche during AML onset and progression plays a crucial role in therapy responsiveness. Circular RNAs have been found to be relevant in AML progression and chemoresistance, influencing cell-cell communication within the leukemic microenvironment.
Article
Biochemistry & Molecular Biology
Anna Benedetti, Chiara Turco, Giulia Fontemaggi, Francesco Fazi
Summary: Non-coding RNAs (ncRNAs) play a pivotal role in regulating the tumor microenvironment (TME) and influencing tumor development and progression by controlling gene expression at multiple levels. Through the production of different ncRNAs, cancer cells orchestrate the recruitment, polarization, and functional changes of tumor-associated macrophages (TAMs), which promote tumorigenesis and facilitate cancer progression and metastasis.