期刊
RHEUMATOLOGY
卷 47, 期 6, 页码 809-814出版社
OXFORD UNIV PRESS
DOI: 10.1093/rheumatology/ken056
关键词
intervertebral disc; interleukin-1; interleukin-1Ra; tumour necrosis factor; in situ zymography; matrix degradation
类别
资金
- Biotechnology and Biological Sciences Research Council Funding Source: Medline
- Medical Research Council Funding Source: Medline
- Wellcome Trust [063022/Z/0S, 057601/Z/99] Funding Source: Medline
Objective. To establish if IL-1 or TNF regulates matrix degradation in the non-degenerate or degenerate intervertebral disc (IVD). Methods. In situ zymography (ISZ) has been used to investigate the role of IL-1 and TNF in the matrix degradation characterizing symptomatic IVD degeneration. ISZ employed three substrates (gelatin, collagen II, casein) and four different challenges, IL-1 beta, IL-1 receptor antagonist (IL-1Ra), TNF-alpha and anti-TNF. Results. We have shown for the first time that whilst IL-1 beta will stimulate and IL-1 receptor antagonist will inhibit matrix degradation in intact human IVD tissue, neither TNF-alpha nor anti-TNF have any measurable effect on degradation of these matrices. Conclusion. This study has addressed a current area of controversy in IVD biology, namely, whether either IL-1 or TNF or both are involved in driving matrix degradation. Our data indicate that IL-1 is a key cytokine mediating matrix degradation in the IVD and therefore a therapeutic target.
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