期刊
ONCOTARGET
卷 6, 期 24, 页码 20404-20418出版社
IMPACT JOURNALS LLC
DOI: 10.18632/oncotarget.4057
关键词
long non-coding RNA (LncRNA); AFAP1 antisense RNA1 (AFAP1-AS1); nasopharyngeal carcinoma (NPC); metastasis; prognosis
资金
- National Natural Science Foundation of China [81172189, 81272298, 81372907, 81301757, 81472531, 81402009]
- Natural Science Foundation of Hunan Province [14JJ1010, 2015JJ1022]
Altered expression of long noncoding RNAs (lncRNAs) associated with human carcinogenesis. We performed a cDNA microarray analysis of lncRNA expression in 12 cases of nasopharyngeal carcinoma (NPC) and 4 non-tumor nasopharyngeal epitheliums. One lncRNA, actin filament associated protein 1 antisense RNA1 (AFAP1-AS1), was identified and selected for further study. AFAP1-AS1 expression was upregulated in NPC and associated with NPC metastasis and poor prognosis. In vitro experiments demonstrated that AFAP1-AS1 knockdown significantly inhibited the NPC cell migration and invasive capability. AFAP1-AS1 knockdown also increased AFAP1 protein expression. Proteomic and bioinformatics analyses suggested that AFAP1-AS1 affected the expression of several small GTPase family members and molecules in the actin cytokeratin signaling pathway. AFAP1-AS1 promoted cancer cell metastasis via regulation of actin filament integrity. AFAP1-AS1 might be a potential novel marker that can predict cancer patient prognosis and as a potential therapeutic target for NPC.
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