Article
Cell Biology
Mengna Zhang, Lingxian Zhang, Ajun Geng, Xiao Li, Yu Zhou, Liming Xu, Yi Arial Zeng, Jinpeng Li, Cheguo Cai
Summary: In this study, the researchers investigated the function of cyclin-dependent kinase 14 (CDK14) in mammary development and breast cancer progression. The results showed that CDK14 is expressed in mammary basal cells and elevated in triple negative breast cancer (TNBC). CDK14 knockdown or inhibition was found to suppress mammary regeneration and the progression of TNBC.
Article
Medicine, General & Internal
Samantha L. Payne, Priyanka Ram, Deepti H. Srinivasan, Thanh T. Le, Michael Levin, Madeleine J. Oudin
Summary: This study reveals that altering the resting membrane potential (RMP) of triple-negative breast cancer cells through manipulating potassium channel expression increases invasion and metastasis, accompanied by changes in gene expression related to cell adhesion. The study also identifies a new strategy to target metastatic breast cancer by repurposing an FDA-approved potassium channel blocker. These findings demonstrate that bioelectricity regulates cancer cell invasion and metastasis, potentially leading to new therapeutics for patients with metastatic disease.
Article
Biochemistry & Molecular Biology
Duaa Alkaabi, Kholoud Arafat, Shahrazad Sulaiman, Aya Mudhafar Al-Azawi, Samir Attoub
Summary: Triple-negative breast cancer (TNBC) is a highly aggressive type of breast cancer with poor prognosis. This study investigated the role of programmed death-ligand 1 (PD-L1) in TNBC MDA-MB-231 cells independent of its binding to PD-1 receptors on T cells. The study found that knockout of PD-L1 inhibited cell proliferation, colony formation, migration, and invasion in vitro, as well as tumor growth in a chick embryo model in vivo. PD-L1 knockout also affected the expression of several downstream proteins involved in tumor progression. These findings suggest that targeting PD-L1 could be a potential therapeutic strategy for TNBC.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Cell Biology
Anli Yang, Fu Peng, Lewei Zhu, Xing Li, Shunling Ou, Zhongying Huang, Song Wu, Cheng Peng, Peng Liu, Yanan Kong
Summary: Melatonin treatment downregulated FUNDC1 and lnc049808 in TNBC cell lines, which inhibited cell proliferation, invasion, and metastasis. lnc049808 and FUNDC1 acted as competing endogenous RNAs binding to miR-101, indicating a potential therapeutic pathway for TNBC progression inhibition by melatonin.
CELL DEATH & DISEASE
(2021)
Article
Cell Biology
Yao Li, Xue Meng, Yuqing Luo, Shuai Luo, Jin Li, Jiafei Zeng, Xiang Huang, Jinjing Wang
Summary: The study found that miR-429 is overexpressed in triple-negative breast cancer and promotes the development of breast cancer by degrading the tumor suppressor DLC1.
Article
Cell Biology
Juliana Haydee Enrique Steinberg, Fabiana Alejandra Rossi, Roberto Magliozzi, Laurensia Yuniati, Matteo Santucci, Mario Rossi, Daniele Guardavaccaro, Angela Lauriola
Summary: The research demonstrates that SHARP1 functions as a suppressor of metastasis in TNBC. It inhibits the invasive phenotype of TNBC by blocking hypoxia-inducible factors. Additionally, targeting the beta TrCP-dependent degradation of SHARP1 shows potential as a therapeutic strategy in TNBC.
CELL DEATH & DISEASE
(2023)
Article
Oncology
Ingrid Espinoza, Chandra Kurapaty, Cheol-Hong Park, Travis Vander Steen, Celina G. Kleer, Elizabeth Wiley, Alfred Rademaker, Elisabet Cuyas, Sara Verdura, Maria Buxo, Carol Reynolds, Javier A. Menendez, Ruth Lupu
Summary: Triple-negative/basal-like breast cancer is characterized by aggressive biological features. Targeting CCN1/CYR61 may have therapeutic value in suppressing the biological aggressiveness of this type of breast cancer.
AMERICAN JOURNAL OF CANCER RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Sarra Bouaouiche, Silvia Ghione, Randa Sghaier, Olivier Burgy, Cindy Racoeur, Valentin Derangere, Ali Bettaieb, Stephanie Plenchette
Summary: The study demonstrates that glyceryl trinitrate (GTN) inhibits the migration and invasion of triple-negative breast cancer cells by supressing the activation of the JAK2/STAT3 signaling pathway. This suggests a potential application of GTN in the treatment of breast cancer, providing a new research direction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Jiahui Mou, Xiaoding Xu, Feifei Wang, Weiwen Kong, Jing Chen, Jin Ren
Summary: In this study, we demonstrated that HMGN4 regulates the proliferation of TNBC through the STAT3 signaling pathway, suggesting its potential as a novel target for anti-TNBC therapy.
Article
Biochemistry & Molecular Biology
Qiuping Xu, Jingwei Zhang, Brian A. Telfer, Hao Zhang, Nisha Ali, Fuhui Chen, Blanca Risa, Adam J. Pearson, Wei Zhang, Katherine G. Finegan, Ahmet Ucar, Emanuele Giurisato, Cathy Tournier
Summary: Clinical evidence shows that dysregulation of ERK5 is common in human breast cancer, with high levels predicting metastatic behavior. ERK5 is essential for maintaining invasive capability of TNBC cells through FAK activation, potentially offering a therapeutic target for aggressive breast cancer.
Article
Oncology
Kurt W. Evans, Erkan Yuca, Stephen S. Scott, Ming Zhao, Natalia Paez Arango, Christian X. Cruz Pico, Turcin Saridogan, Maryam Shariati, Caleb A. Class, Christopher A. Bristow, Christopher P. Vellano, Xiaofeng Zheng, Ana Maria Gonzalez-Angulo, Xiaoping Su, Coya Tapia, Ken Chen, Argun Akcakanat, Bora Lim, Debu Tripathy, Timothy A. Yap, Maria Emilia Di Francesco, Giulio F. Draetta, Philip Jones, Timothy P. Heffernan, Joseph R. Marszalek, Funda Meric-Bernstam
Summary: Oxidative phosphorylation is a metabolic vulnerability in triple-negative breast cancer, and inhibiting it with IACS-10759 may enhance efficacy of multiple targeted therapies.
Article
Oncology
Sainan Liu, Bin Liu, Qian Zhao, Jikang Shi, Yulu Gu, Yanbo Guo, Yong Li, Yunkai Liu, Yi Cheng, Yichun Qiao, Yawen Liu
Summary: The study found that low FST expression is associated with poor prognosis of patients with TNBC, and FST exhibits heterogeneous roles in apoptosis in different subtypes of TNBC cells.
CANCER CELL INTERNATIONAL
(2021)
Article
Cell Biology
Seogho Son, Hyungjoo Kim, Hogeun Lim, Joo-hyung Lee, Kyung-min Lee, Incheol Shin
Summary: In this study, the overexpression of CCN3 in TNBC patients was found to be associated with unfavorable outcomes. CCN3 knockdown reduced cancer stem cell formation, metastasis, and tumor growth by inhibiting the EGFR/MAPK pathway. Furthermore, CCN3 was found to activate the Wnt signaling pathway, leading to increased expression of GPNMB.
CELL DEATH & DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Lijuan Guo, Wanjun Zhang, Xue Zhang, Jun Wang, Jiaqi Nie, Xiaomeng Jin, Ying Ma, Shi Wang, Xinhong Zhou, Yilei Zhang, Yan Xu, Yoshimasa Tanaka, Jingping Yuan, Xing-Hua Liao, Yiping Gong, Li Su
Summary: In this study, SIPA1 was found to function as a transcription factor that regulates the expression of genes involved in cell growth, differentiation, and response to environmental factors. Importin β1 was identified as an interacting partner of SIPA1 during fibronectin treatment. SIPA1's DNA-binding region (DBR) recognized and bound to a TGAGTCAB motif, and its transcriptional regulation was shown to be necessary for the migration and invasion of triple-negative breast cancer cells.
Article
Oncology
Lianmei Zhang, Yang Zhao, Jing Yang, Yaning Zhu, Ting Li, Xiaoyan Liu, Pengfei Zhang, Jingliang Cheng, Suan Sun, Chunli Wei, Junjiang Fu
Summary: In this study, the expression of CTSL in TNBC tissues and adjacent tissues was monitored and analyzed for its correlation with clinicopathological characteristics. The results showed that CTSL levels were higher in TNBC and correlated with differentiation, TNM stage, tumor size, and lymph node metastasis. Moreover, in vitro experiments demonstrated that CTSL overexpression enhanced proliferation, migration, and invasion abilities in MCF-7 and MDA-MB-231 cell lines. Conversely, knockdown of CTSL decreased these characteristics in MDA-MB-231 cell line. The findings suggest that CTSL could serve as a potential therapeutic and prognostic target for TNBC.
FRONTIERS IN ONCOLOGY
(2023)
Article
Geriatrics & Gerontology
Lucia Jimenez, Andreia Silva, Giampaolo Calissi, Ines Grenho, Rita Monteiro, Victor Mayoral-Varo, Carmen Blanco-Aparicio, Joaquin Pastor, Victor Bustos, Franz Bracher, Diego Megias, Bibiana Ferreira, Wolfgang Link
Summary: Various compounds including resveratrol, piperlongumine, and harmine were found to induce FOXO activity, with piperlongumine and harmine activating transcription. These compounds were shown to affect FOXO nuclear translocation and inhibiting ROS production, with molecular mechanisms involving DYRK1A inhibition and SIRTs reverting the effects of harmine on FOXO3 activity, independently of PI3K/AKT signaling and CRM1-mediated nuclear export.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2022)
Article
Biotechnology & Applied Microbiology
Giuseppina Bozzuto, Giuseppe D'Avenio, Maria Condello, Simona Sennato, Ezio Battaglione, Giuseppe Familiari, Agnese Molinari, Mauro Grigioni
Summary: This study compared the use of Electric Cell-substrate Impedance-Sensing (ECIS) with traditional cytotoxicity tests, finding that ECIS can provide continuous and noninvasive monitoring of cell behavior, potentially offering advantages in studying ZnO NPs toxicity. Furthermore, impedance curve trends were able to distinguish between different cell death mechanisms, offering cost and time advantages.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Review
Oncology
Roberto Corchado-Cobos, Natalia Garcia-Sancha, Marina Mendiburu-Elicabe, Aurora Gomez-Vecino, Alejandro Jimenez-Navas, Manuel Jesus Perez-Baena, Marina Holgado-Madruga, Jian-Hua Mao, Javier Canueto, Sonia Castillo-Lluva, Jesus Perez-Losada
Summary: Metabolic changes in tumor cells and their interactions with stromal cells play a crucial role in tumor growth and progression. Understanding these metabolic changes can help identify new treatment strategies. Breast cancer serves as a useful example to illustrate the metabolic alterations in cancer.
Article
Biochemistry & Molecular Biology
Fatima Milhano Santos, Joana Mesquita, Joao Paulo Castro-de-Sousa, Sergio Ciordia, Alberto Paradela, Candida Teixeira Tomaz
Summary: Oxidative stress refers to the imbalance between pro-oxidants and antioxidants, leading to various retinal disorders. While the human body has defense mechanisms to counteract autoxidation, it may not always be sufficient to prevent cellular damage. Modern lifestyles and environmental factors contribute to increased chemical exposure and stress induction, further exacerbating oxidative stress.
Article
Biochemistry & Molecular Biology
Maria Ovejero-Sanchez, Jorge Rubio-Heras, Maria del Carmen Vicente de la Pena, Laura San-Segundo, Jesus Perez-Losada, Rogelio Gonzalez-Sarmiento, Ana Belen Herrero
Summary: Ovarian cancer is a deadly malignancy that requires more effective treatments. The combination of chloroquine with nonhomologous end joining inhibitors has been found to inhibit proliferation and induce apoptosis in ovarian cancer cell lines. Additionally, the combination with a pan-histone deacetylase inhibitor enhances the efficacy of chloroquine. These drug combinations could represent new therapeutic strategies against ovarian cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Annarita Stringaro, Stefano Serra, Alessandro Gori, Annarica Calcabrini, Marisa Colone, Maria Luisa Dupuis, Francesca Spadaro, Serena Cecchetti, Alberto Vitali
Summary: This study analyzed the cell specificity, cell viability effects, intracellular distribution, and immune cell response of a new peptide conjugate of Aloe-emodin in SKBR3 and A549 cell lines. The results showed that the conjugate was more effective at reducing cell viability than AE in both cell lines, with a nuclear localization in SKBR3 cells and cytoplasmic distribution in A459 cells.
Review
Biochemistry & Molecular Biology
Natalia Garcia-Sancha, Roberto Corchado-Cobos, Aurora Gomez-Vecino, Alejandro Jimenez-Navas, Manuel Jesus Perez-Baena, Adrian Blanco-Gomez, Marina Holgado-Madruga, Jian-Hua Mao, Javier Canueto, Sonia Castillo-Lluva, Marina Mendiburu-Elicabe, Jesus Perez-Losada
Summary: Metabolic changes play a crucial role in tumor growth and also occur during the physiological growth of tissues. Tumors and tissue repair share similar molecular, cellular, and tissue mechanisms, which are integrated within a Systems Biology framework. The polygenic component of complex traits is largely unknown, including in the context of cancer.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Lucia Jimenez, Carlos Amenabar, Victor Mayoral-Varo, Thomas A. Mackenzie, Maria C. Ramos, Andreia Silva, Giampaolo Calissi, Ines Grenho, Carmen Blanco-Aparicio, Joaquin Pastor, Diego Megias, Bibiana Ferreira, Wolfgang Link
Summary: FOXO proteins play important roles in cancer and aging, and their activity can be regulated through pharmacological manipulation. The study suggests that mTORC2 is the most important second layer kinase negatively regulating FOXO activity.
Article
Biochemistry & Molecular Biology
Andrew J. J. McGovern, Maria Angeles Arevalo, Sergio Ciordia, Luis Miguel Garcia-Segura, George E. E. Barreto
Summary: The existence of sex differences in disease incidence is partly due to sex differences in metabolism. These differences may be subtle and influenced by multiple sex-specific factors. Hormone interactions may regulate Alzheimer's disease and the electron transport chain-associated pathways by altering the expression of respiratory proteins.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Lucia Jimenez, Victor Mayoral-Varo, Carlos Amenabar, Judit Ortega, Joao G. N. Sequeira, Miguel Machuqueiro, Cristiana Mourato, Romano Silvestri, Andrea Angeli, Fabrizio Carta, Claudiu T. Supuran, Diego Megias, Bibiana I. I. Ferreira, Wolfgang Link
Summary: Chromosomal region maintenance 1 (CRM1), also known as Xpo1 and exportin-1, plays a crucial role in nuclear export and has been identified as a potential therapeutic target for viral infections and cancer. Inhibition of CRM1 has shown promise in treating multiple myeloma, but the currently available inhibitor, selinexor, has dose-limiting toxicity. In this study, the researchers developed a CRM1 inhibitor discovery platform and identified an active organoselenium compound.
Article
Multidisciplinary Sciences
Javier Glez-Vaz, Arantza Azpilikueta, Maria C. Ochoa, Irene Olivera, Gabriel Gomis, Asunta Cirella, Carlos Luri-Rey, Maite alvarez, Jose. L. L. Perez-Gracia, Sergio Ciordia, Inaki Eguren-Santamaria, Raluca Alexandru, Pedro Berraondo, Carlos de Andrea, Alvaro Teijeira, Fernando Corrales, Juan. M. M. Zapata, Ignacio Melero
Summary: CD137 is a member of the TNFR family that mediates potent T cell costimulatory signals upon ligation by CD137L or agonist monoclonal antibodies (mAbs). The physical association between cIAP1/cIAP2 and the CD137 signaling complex is demonstrated. cIAPs are required for CD137 signaling towards NF-κB and MAPK pathways, and for the costimulation of human and mouse T lymphocytes.
Review
Oncology
Manuel Jesus Perez-Baena, Francisco Josue Cordero-Perez, Jesus Perez-Losada, Marina Holgado-Madruga
Summary: GAB1 is a pleckstrin homology domain-containing protein that plays a crucial role in cellular transformation and cancer progression, activating various signaling pathways and modulating resistance to anticancer therapies. Its deficiency leads to developmental abnormalities, while its dysregulation is associated with worse prognosis in multiple cancer types. Understanding the mechanisms of GAB1 in cancer could provide insights for future anticancer strategies.
Article
Cell Biology
Aurora Gomez-Vecino, Roberto Corchado-Cobos, Adrian Blanco-Gomez, Natalia Garcia-Sancha, Sonia Castillo-Lluva, Ana Martin-Garcia, Marina Mendiburu-Elicabe, Carlos Prieto, Sara Ruiz-Pinto, Guillermo Pita, Alejandro Velasco-Ruiz, Carmen Patino-Alonso, Purificacion Galindo-Villardon, Maria Linarejos Vera-Pedrosa, Jose Jalife, Jian-Hua Mao, Guillermo Macias de Plasencia, Andres Castellanos-Martin, Maria del Mar Saez-Freire, Susana Fraile-Martin, Telmo Rodrigues-Teixeira, Carmen Garcia-Macias, Julie Milena Galvis-Jimenez, Asuncion Garcia-Sanchez, Maria Isidoro-Garcia, Manuel Fuentes, Maria Begona Garcia-Cenador, Francisco Javier Garcia-Criado, Juan Luis Garcia-Hernandez, Maria Angeles Hernandez-Garcia, Juan Jesus Cruz-Hernandez, Cesar Augusto Rodriguez-Sanchez, Alejandro Martin Garcia-Sancho, Estefania Perez-Lopez, Antonio Perez-Martinez, Federico Gutierrez-Larraya, Antonio J. Carton, Jose Angel Garcia-Saenz, Ana Patino-Garcia, Miguel Martin, Teresa Alonso-Gordoa, Christof Vulsteke, Lieselot Croes, Sigrid Hatse, Thomas Van Brussel, Diether Lambrechts, Hans Wildiers, Hang Chang, Marina Holgado-Madruga, Anna Gonzalez-Neira, Pedro L. Sanchez, Jesu Perez Losada
Summary: Cardiotoxicity due to anthracyclines (CDA) affects cancer patients, and identifying individuals at risk is challenging. In this study, the authors investigated intermediate molecular phenotypes (IMPs) associated with myocardial damage to determine CDA susceptibility. By analyzing a mouse cohort treated with doxorubicin and docetaxel, they identified genetic markers linked to IMPs and CDA. These markers were also found to be associated with CDA in cancer patients, highlighting their potential as predictive factors. Additionally, genetic risk scores were generated using machine-learning regression to personalize patient management.
Review
Oncology
Madison N. Wicker, Kay-Uwe Wagner
Summary: Cellular plasticity, the ability of cells to change their identity, plays a crucial role in mammary gland development and breast cancer progression. This review provides a comprehensive overview of the factors and mechanisms that promote cellular plasticity in the mammary gland. It discusses changes in cell identity during normal development, the role of the gestation cycle, and highlights the importance of the microenvironment and extracellular matrix. The review also explores cellular reprogramming during mammary tumorigenesis, focusing on the origin of basal-like breast cancers and the role of oncogenic signaling networks. Additionally, recent advances in genetically engineered models to study cellular plasticity in vivo are discussed.
Meeting Abstract
Gastroenterology & Hepatology
Alex Claveria-Cabello, Jose Maria Herranz, Elena Adan-Villaescusa, Maria U. Latasa, Maria Arechederra, Iker Uriarte, Antonio A. Pineda, Pedro Berraondo, Bruno Sangro, Jose Marin, Maria Luz Martinez-Chantar, Sergio Ciordia, Fernando Corrales, Jessica Zucman-Rossi, Emilie Indersie, Stefano Cairo, Montserrat Domingo-Sabat, Carmen Berasain, Maite G. Fernandez-Barrena, Matias A. Avila
JOURNAL OF HEPATOLOGY
(2023)