Article
Microbiology
Cristina Lasarte-Monterrubio, Paula Guijarro-Sanchez, Juan Carlos Vazquez-Ucha, Isaac Alonso-Garcia, Laura Alvarez-Fraga, Michelle Outeda, Marta Martinez-Guitian, Andrea Pena-Escolano, Romina Maceiras, Emilio Lence, Concepcion Gonzalez-Bello, Jorge Arca-Suarez, German Bou, Alejandro Beceiro
Summary: Resistance to cefiderocol has emerged among carbapenemase-producing Enterobacterales, particularly in the Enterobacter cloacae complex. This resistance is mainly due to the production of VIM-1 carbapenemase and may also involve the co-production of SHV-12 and inactivation of the FcuA-like siderophore receptor.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2023)
Article
Microbiology
Edgar Campos-Madueno, Aline Moser, Martin Risch, Thomas Bodmer, Andrea Endimiani
Summary: The dissemination of plasmid-mediated carbapenemases within Klebsiella oxytoca and Klebsiella grimontii differs, with more research on the former than the latter. Whole-genome sequencing revealed that K. grimontii strains carry various plasmids, some of which are associated with other Enterobacterales species.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Remy A. Bonnin, Delphine Girlich, Agnes B. Jousset, Cecile Emeraud, Elodie Creton, Lauraine Gauthier, Thomas Jove, Laurent Dortet, Thierry Naas
Summary: This study describes the first occurrence of Tn1332 in Enterobacterales and the phenotypic diversity of VIM-2-producing E. hormaechei. The plasmid carrying bla(VIM-2) exhibited phenotypic diversity among the isolates, with differences in insertion location and relatedness.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2021)
Review
Infectious Diseases
Ting-Kuang Yeh, Hsueh-Ju Lin, Po-Yu Liu, Jui-Hsing Wang, Po-Ren Hsueh
Summary: Enterobacter hormaechei, a member of the Enterobacter cloacae complex, has emerged as an important pathogen in healthcare-associated infections worldwide, with the advancements in phylogenetic analyses and whole-genome sequencing techniques contributing to its identification.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Microbiology
Marina R. Pulido, Andrea Garcia-Montaner, Lorena Lopez-Cerero, Felipe Fernandez-Cuenca, Jose Gutierrez-Fernandez, Alvaro Pascual
Summary: This study characterized a new genetic structure containing a variant of bla(VIM-2), named bla(VIM-63), in Citrobacter freundii and Pseudomonas aeruginosa strains. The bla(VIM-63) gene was found to be stably integrated in the chromosome and showed similar susceptibility patterns to carbapenems as bla(VIM-2). This is the first report of a stable multimer of a metallo-beta-lactamase and provides important insights into the mechanisms of drug resistance.
JOURNAL OF BACTERIOLOGY
(2022)
Article
Infectious Diseases
Jose Raul Nieto-Saucedo, Luis Esau Lopez-Jacome, Rafael Franco-Cendejas, Claudia Adriana Colin-Castro, Melissa Hernandez-Duran, Luis Raul Rivera-Garay, Karina Senyase Zamarripa-Martinez, Juan Luis Mosqueda-Gomez
Summary: The prevalence of carbapenem-resistant Gram-negative bacilli (CR-GNB) and the frequency of carbapenemase-encoding genes were evaluated in a tertiary referral center in El Bajio, Mexico. Among 508 Gram-negative bacilli, 37 were carbapenem-resistant (7.3%), and only 15 (40.5%) of them carried carbapenemase-encoding genes.
Article
Microbiology
Zishuo Cheng, Christopher R. Bethel, Pei W. Thomas, Ben A. Shurina, John-Paul Alao, Caitlyn A. Thomas, Kundi Yang, Steven H. Marshall, Huan Zhang, Aidan M. Sturgill, Andrea N. Kravats, Richard C. Page, Walter Fast, Robert A. Bonomo, Michael W. Crowder
Summary: Metallo-beta-lactamases (MBLs) are a growing clinical threat as they inactivate most beta-lactam antibiotics. Variants of the IMP-1 gene exhibit increased resistance to carbapenems but do not confer additional survival advantage in limited zinc availability. Evolution of MBL subfamilies like IMP-6, -10, and -78 appears to be driven by different selective pressures.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Public, Environmental & Occupational Health
Vered Schechner, Katya Levytskyi, Ohad Shalom, Alexander Yalek, Amos Adler
Summary: This study described an hospital-wide outbreak of IMI-Producing Enterobacter ludwigii, originated in the community and subsequently disseminated inside the institution. Most patients were identified to be colonized within 72 hours of admission, with no clear history of carriage of other multi-drug resistant bacteria.
ANTIMICROBIAL RESISTANCE AND INFECTION CONTROL
(2021)
Article
Infectious Diseases
Charlotte Nielsen Agergaard, Lone Jannok Porsbo, Thomas Vognbjerg Sydenham, Sanne Gronvall Kjaer Hansen, Kat Steinke, Sanne Lokkegaard Larsen, Kristjan Orri Helgason, Frank Hansen, Kasper Thystrup Karstensen, Anna E. Henius, Barbara Juliane Holzknecht, Lillian Soes, Kristian Schonning, Mikala Wang, Nina Ank, Anna Margret Halldorsdottir, Olafur Guolaugsson, Anette M. Hammerum, Anne Kjerulf, Brian Kristensen, Henrik Hasman, Ulrik Stenz Justesen
Summary: From October 2022 through January 2023, nine patients in Denmark and one patient in Iceland were infected with NDM-5/OXA-48-carbapenemase-producing Enterobacter hormaechei ST79. There were no nosocomial links among the patients, but they had all been treated with dicloxacillin capsules. The same strain of NDM-5/OXA-48-carbapenemase-producing E. hormaechei ST79 was found on the surface of dicloxacillin capsules in Denmark, indicating that they were the source of the outbreak. Special attention should be paid to detecting the outbreak strain in the microbiology laboratory.
Article
Infectious Diseases
Aura Falco, Daniela Guerrero, Isabella Garcia, Adriana Correa, Sandra Rivera, Maria Beatriz Olaya, Carlos Aranaga
Summary: The study aimed to molecularly characterize 28 isolates of the E. cloacae complex resistant to cephalosporins and carbapenems from five hospitals in Cali, Colombia. It found that 43% of the isolates carried the bla(KPC-2) gene variant. High genetic diversity was observed among isolates, with the most common sequence type being ST510.
Article
Infectious Diseases
Shixing Liu, Na Huang, Cui Zhou, Yishuai Lin, Ying Zhang, Lingbo Wang, Xiangkuo Zheng, Tieli Zhou, Zhongyong Wang
Summary: The study revealed that carbapenem-nonsusceptible ECC strains producing non-carbapenemase were predominant, and their resistance mechanisms were clarified. In addition, the overexpression of efflux pump was also associated with carbapenem resistance. The MLST results demonstrated the genetic diversity of ECC strains.
INFECTION AND DRUG RESISTANCE
(2021)
Article
Microbiology
Willames M. B. S. Martins, Evelin R. Martins, Leticia K. de Andrade, Refath Farzana, Timothy R. Walsh, Mark A. Toleman, Mara C. L. Nogueira, Ana C. Gales
Summary: This study characterized a multidrug-resistant Enterobacter spp. isolate from a patient in a Brazilian tertiary hospital in 2014, showing the presence of new beta-lactamase variants and a mobile colistin resistance gene.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Shikai Wu, Yanling He, Yu Feng, Zhiyong Zong
Summary: We identified a clinical strain of Enterobacter that was resistant to carbapenems but susceptible to cefotaxime and ceftazidime. The presence of the rare carbapenemase-encoding gene bla(FRI-11) on a plasmid containing two replicons, IncFII(pECLA) and IncFIA(HI1), sparked further investigation. The FRI carbapenemases, including FRI-11, FRI-2, FRI-3, FRI-4, FRI-6, FRI-7, and FRI-9, belong to the same group based on amino acid sequence similarity and are carried by plasmids containing an IncFII(pECLA) replicon. Awareness should be raised regarding plasmid-encoded FRI carbapenemases that confer an unusual carbapenem-resistant but 3rd-generation-cephalosporin-susceptible resistance profile.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Microbiology
T. Blanco-Martin, J. Guzman-Puche, C. Riazzo, M. Gasca-Santiyan, M. Hernandez-Garcia, R. Canton, J. Torre-Cisneros, C. Herrera, L. Martinez-Martinez
Summary: IMI carbapenemases confer an unusual resistance pattern, making them difficult to detect. Commercial molecular methods usually do not include bla(IMI) genes, contributing to the hidden dissemination of bacteria producing these enzymes. We report a plasmid-encoded IMI-6 carbapenemase in an Enterobacter ludwigii isolate, providing insights into its genetic characteristics.
MICROBIOLOGY SPECTRUM
(2023)
Article
Microbiology
Maria Guadalupe Martinez-Zavaleta, Diana Fernandez-Rodriguez, Melissa Hernandez-Duran, Claudia A. Colin-Castro, Maria de Lourdes Garcia-Hernandez, Noe Becerra-Lobato, Rafael Franco-Cendejas, Luis Esau Lopez-Jacome
Summary: This study aimed to characterize carbapenemase production in clinical strains of Pseudomonas aeruginosa. The most frequently found carbapenemases were VIM and GES, followed by IMP. Metallo-ss-lactamases were the most prevalent among the identified carbapenemase genes. Searching for carbapenemase genes in resistant isolates is crucial for preventing transmission and guiding antimicrobial treatment.
Review
Fisheries
Carlos Fajardo, Marcos De Donato, Rafael Carrasco, Gonzalo Martinez-Rodriguez, Juan Miguel Mancera, Francisco Javier Fernandez-Acero
REVIEWS IN AQUACULTURE
(2020)
Review
Biochemistry & Molecular Biology
Carlos Fajardo, Marcos De Donato, Hectorina Rodulfo, Gonzalo Martinez-Rodriguez, Benjamin Costas, Juan Miguel Mancera, Francisco Javier Fernandez-Acero
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2020)
Article
Agriculture, Dairy & Animal Science
James A. Adeyemi, Sunday O. Peters, Marcos De Donato, Andres Pech Cervantes, Ibukun M. Ogunade
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2020)
Article
Immunology
Sunday O. Peters, Tanveer Hussain, Adeyemi S. Adenaike, Jordan Hazzard, Olanrewaju B. Morenikeji, Marcos De Donato, Sujay Paul, Masroor Babar, Abdulmojeed Yakubu, Ikhide G. Imumorin
FRONTIERS IN IMMUNOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Sujay Paul, Luis M. Ruiz-Manriquez, S. Janin Ledesma-Pacheco, Javier A. Benavides-Aguilar, Andrea Torres-Copado, Jonathan I. Morales-Rodriguez, Marcos De Donato, Aashish Srivastava
Summary: MiRNAs are small non-coding RNA molecules that play a crucial role in chronic pediatric diseases such as bronchial asthma, congenital heart diseases, cystic fibrosis, type 1 diabetes mellitus, and epilepsy, serving as potential novel biomarkers for diagnosis and prognosis.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2021)
Article
Engineering, Environmental
Alexandra Calvo-Olvera, Marcos De Donato-Capote, Hector Pool, Norma G. Rojas-Avelizapa
Summary: This study evaluated the toxic effects of chemically and biologically synthesized cadmium sulfide quantum dots on bacteria and plants, revealing that the production route significantly impacts their toxicity, with biogenically synthesized CdSQDs showing less toxicity.
JOURNAL OF ENVIRONMENTAL SCIENCE AND HEALTH PART A-TOXIC/HAZARDOUS SUBSTANCES & ENVIRONMENTAL ENGINEERING
(2021)
Review
Fisheries
Carlos Fajardo, Gonzalo Martinez-Rodriguez, Benjamin Costas, Juan Miguel Mancera, Sergio Fernandez-Boo, Hectorina Rodulfo, Marcos De Donato
Summary: Researchers have conducted meta-analysis on the crustacean immune system using transcriptomic analysis and databases, revealing the impact of pathogens, abiotic stressors, dietary factors, and beneficial microbes on genes and metabolic pathways. Efforts are needed to develop genomic tools for commercial applications aiming to improve culture conditions and develop more disease-resistant strains.
REVIEWS IN AQUACULTURE
(2022)
Article
Environmental Sciences
Ana K. Tapia-Arreola, Daniel A. Ruiz-Garcia, Hectorina Rodulfo, Ashutosh Sharma, Marcos De Donato
Summary: This study determined the frequency and resistance genes of antimicrobial-resistant Gram-negative bacteria in the Lerma River basin. The results showed high variation in bacterial isolates and low diversity among resistant bacteria. C. freundii, E. coli, and S. marcescens were the predominant species, and resistance to beta-lactams, quinolones, and aminoglycosides was frequently observed. Whole genome sequencing revealed shared virulence and antimicrobial resistance genes in S. marcescens and K. pneumoniae, while E. coli had the most unique genes. The contamination of the Lerma River with multidrug-resistant strains carrying various resistance genes highlights the need for environmental authorities to assess and regulate sanitary conditions in the river.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Agriculture, Dairy & Animal Science
Judith Wilson, Marcos De Donato, Brooke Appelbaum, Carly Turner Garcia, Sunday Peters
Summary: Overtraining syndrome (OTS) is caused by excessive training and inadequate recovery, leading to decreased performance and impaired repair of damage. Acute, intense physical exercise affects the expression of immune genes, and can be used to evaluate OTS indications.
Article
Medicine, Research & Experimental
Hectorina Rodulfo, Milagro Horta, Genova Mata, Rafela Gutierrez, Yarmilia Gonzalez, Elvia Michelli, Militza Guzman, Dianny Martinez, Ashutosh Sharma, Marcos De Donato
Summary: The study in two main hospitals in Eastern Venezuela revealed the presence of P. mirabilis in both intrahospital and community-acquired infections, with a high frequency of multidrug resistant strains. A particular hospital also showed strains carrying both bla(CTX-M) and bla(TEM) genes. Infections by multidrug-resistant/extensively drug-resistant P. mirabilis strains were identified as a public health issue that requires effective control and prevention measures.
INVESTIGACION CLINICA
(2021)
Article
Public, Environmental & Occupational Health
Dianny Martinez, Luisa Cana, Hectorina Rodulfo, Jose Garcia, Diorelis Gonzalez, Lucy Rodriguez, Marcos De Donato
REVISTA PANAMERICANA DE SALUD PUBLICA-PAN AMERICAN JOURNAL OF PUBLIC HEALTH
(2020)
Article
Agriculture, Dairy & Animal Science
Olanrewaju B. Morenikeji, Oyeyemi O. Ajayi, Sunday O. Peters, Fidalis D. Mujibi, Marcos De Donato, Bolaji N. Thomas, Ikhide G. Imumorin
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2020)
Article
Infectious Diseases
Hectorina Rodulfo, Anlenys Arcia, Aldo Hernandez, Elvia Michelli, Dianny del Valle Martinez, Militza Guzman, Ashutoch Sharma, Marcos De Donato
REVISTA DO INSTITUTO DE MEDICINA TROPICAL DE SAO PAULO
(2019)
Article
Tropical Medicine
Militza Guzman, Elsa Salazar, Vicmaris Cordero, Ana Castro, Andreina Villanueva, Hectorina Rodulfo, Marcos De Donato
