Article
Biochemistry & Molecular Biology
Lili Niu, Philipp E. Geyer, Rajat Gupta, Alberto Santos, Florian Meier, Sophia Doll, Nicolai J. Wewer Albrechtsen, Sabine Klein, Cristina Ortiz, Frank E. Uschner, Robert Schierwagen, Jonel Trebicka, Matthias Mann
Summary: This study quantified protein composition differences among liver cell types and analyzed dynamic proteome changes in primary cell cultures, providing valuable insights for biological and pharmaceutical research.
MOLECULAR SYSTEMS BIOLOGY
(2022)
Article
Plant Sciences
Shruti Sharma, Renu Deswal
Summary: A gel-free proteome analysis of Dioscorea tuber at different developmental stages identified metabolic changes and novel biological processes. The study revealed specific protein markers for tuber germination and formation, providing insights for improving the productivity of this crop.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Neurosciences
Alexander G. Thompson, Elizabeth Gray, Philip D. Charles, Michele T. M. Hu, Kevin Talbot, Roman Fischer, Benedikt M. Kessler, Martin R. Turner
Summary: This study compared CSF proteomic data from ALS patients, Parkinson's disease patients, and healthy controls, revealing alterations in immune system and axonal modules in ALS, which are associated with TDP-43 dysfunction. Longitudinal analysis showed temporal changes in the CSF proteome in ALS patients. Protein correlation network disruptions in Parkinson's disease did not show any module enrichment.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Nanoscience & Nanotechnology
Renata Rank Miranda, Anny Carolline Silva Oliveira, Lilian Skytte, Kaare Lund Rasmussen, Frank Kjeldsen
Summary: The use of mass spectrometry-based proteomics has provided detailed insights into the role of reactive oxygen species (ROS) in the toxicity of silver nanoparticles (AgNP), showing that AgNP induces ROS generation and inhibits cell proliferation pathways. Additionally, it was found that cells increase their antioxidant pool via the NRF2 pathway as a response to AgNP-induced ROS.
Article
Biochemistry & Molecular Biology
Xianyu Huang, Shan Yang, Jian Sun, Xia Li, Shao-Yu Zhou, Jing-Shan Shi, Jie Liu, Qin Wu
Summary: Dendrobium nobile Lindl. alkaloids (DNLA) have protective effects against chronic alcoholic liver injury by decreasing triglyceride levels, ameliorating hepatocyte degeneration and steatosis, restoring the aberrant gene expression pattern induced by alcohol, and alleviating oxidative stress and xenobiotic metabolism disruption.
Article
Biochemical Research Methods
Huilin Hong, Zhiguang Zhao, Xiahe Huang, Chao Guo, Hui Zhao, Guo-Dong Wang, Ya-Ping Zhang, Jian-Ping Zhao, Jianhui Shi, Qing-Feng Wu, Yong-Hui Jiang, Yingchun Wang, Lei M. Li, Zhuo Du, Yong Q. Zhang, Ying Xiong
Summary: This study analyzed proteomes from six regions of the postnatal brain at three developmental stages of domestic dogs, revealing the correlation between myelination and hippocampus-related proteins in dogs and humans. The global expression patterns of disease-associated proteins in the brain of dogs resemble those in humans more than in mice. These findings provide insights into brain development and function, and can guide mechanistic and translational research using appropriate animal models.
MOLECULAR & CELLULAR PROTEOMICS
(2022)
Article
Neurosciences
Sharad Kumar Suthar, Sang-Yoon Lee
Summary: Despite the ambiguous understanding of the canonical signaling, interaction network molecules, biological functions, and role of alpha-synuclein, the use of artificial intelligence and Bioinformatics tools like ingenuity pathway analysis (IPA) have allowed us to analyze and predict the key pathways and interactions of alpha-synuclein. The study reveals neuroinflammation, Huntington's disease, and sirtuin signaling as the important pathways, and predicts Parkinson's disease as the key toxicity pathway of alpha-synuclein.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Biochemical Research Methods
Libang Yang, Adam Gilbertsen, Karen Smith, Hong Xia, LeeAnn Higgins, Candace Guerrero, Craig A. Henke
Summary: IPF is a progressive fibrotic lung disease with unclear pathogenesis. Through quantitative mass spectrometry and interactomics, nuclear PARP1, CDK1, and BACH1 were identified as key proteins regulating IPF MPC fibrogenicity.
Article
Biochemistry & Molecular Biology
Elena Dozio, Elisa Maffioli, Elena Vianello, Simona Nonnis, Francesca Grassi Scalvini, Leonardo Spatola, Paola Roccabianca, Gabriella Tedeschi, Massimiliano Marco Corsi Romanelli
Summary: This study assessed the impact of a high-fat diet on obesity-related chronic kidney disease (CKD) and identified specific molecular pathways and markers associated with renal injury. The findings suggest that a high-fat diet activates fibrosis pathways and alters lipid metabolism, while early signs of kidney damage may be related to changes in membrane protein expression. These results have potential implications for the early detection and prevention of tissue damage in obesity-related CKD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mariola Slowinska, Lukasz Paukszto, Laura Pardyak, Jan P. Jastrzebski, Ewa Liszewska, Joanna Wisniewska, Krzysztof Kozlowski, Jan Jankowski, Barbara Bilinska, Andrzej Ciereszko
Summary: Through sequencing and mass spectrometry analysis of mature oocytes in birds, important genes and proteins related to the final stage of egg development were identified; the characteristics of the ZP gene subfamilies and the expression sites of ZP1 in the liver and granulosa cells were revealed; the analysis indicated the presence of estrogen receptor signaling pathway and oxidative phosphorylation as a possible source of adenosine triphosphate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Oncology
Lanyang Gao, Danli Zhu, Qin Wang, Zheng Bao, Shigang Yin, Huiyan Qiang, Heinrich Wieland, Jinyue Zhang, Alexander Teichmann, Jing Jia
Summary: USP7 is overexpressed in melanoma and affects specific proteins and processes through multiple signaling pathways, with knockdown of USP7 slowing down melanoma cell proliferation.
FRONTIERS IN ONCOLOGY
(2021)
Article
Cell Biology
Xi Zhang, Yang Liu, Zhen Zhang, Juan Tan, Junjun Zhang, Hao Ou, Jie Li, Zewen Song
Summary: This study investigated the mechanism of a novel angiogenesis inhibitor, anlotinib, against pancreatic cancer cells through multi-omics analyses, and constructed an anlotinib-related gene signature to identify a subgroup of pancreatic cancer patients who might benefit from anlotinib. The results showed that anlotinib exerted significant cytotoxicity on pancreatic cancer cells and had a profound inhibitory effect on ribosome, cell cycle, RNA metabolism, and lysosome.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Michael Fish, Delaney Nash, Alexandru German, Alyssa Overton, Masoud Jelokhani-Niaraki, Simon D. X. Chuong, Matthew D. Smith
Summary: Plastids, specialized organelles in plant cells, originated from ancient cyanobacteria. As genes encoding plastid proteins were transferred to the nuclear genome during evolution, eukaryotic cells developed targeting pathways and protein machinery to direct these proteins back to the plastids. Chloroplasts, the most well-studied plastids, play essential roles in photosynthesis and metabolism. Understanding the pathways and regulation of protein targeting is vital for chloroplast biogenesis and function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Chuan Huang, Hoa-Quynh Pham, Lina Zhu, Rui Wang, Oi-Kwan Law, Shu-Ling Lin, Qi-Chang Nie, Liang Zhang, Xin Wang, Terrence Chi-Kong Lau
Summary: Antibiotic resistance poses a significant threat to global public health. This study found that multidrug-resistant plasmids can influence gene expression and metabolic pathways in bacterial hosts, providing a growth advantage to plasmid-carrying bacteria in the gut. Additionally, the study revealed the versatility of multidrug-resistant plasmids in moderating the cellular gene network, which could be utilized in the development of therapeutics for bacteria carrying these plasmids.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Li Zhang, Enling Wang, Gang Peng, Yi Wang, Feiruo Huang
Summary: Feeding high-energy and low-protein diets in laying hens leads to fatty liver hemorrhagic syndrome (FLHS). The mechanism of hepatic fat accumulation in FLHS-affected hens was investigated through hepatic proteome and acetyl-proteome analysis. The results showed that upregulated proteins were related to fat digestion and absorption, unsaturated fatty acid biosynthesis, and glycerophospholipid metabolism, while downregulated proteins were associated with bile secretion and amino acid metabolism. Acetylated proteins were involved in ribosome and fatty acid degradation, PPAR signaling pathway, and deacetylated proteins were related to valine, leucine, and isoleucine degradation in hens with FLHS. These findings indicate that acetylation inhibits hepatic fatty acid oxidation and transport in FLHS-affected hens, primarily by affecting protein activity. The study offers new nutritional regulation options to alleviate FLHS in laying hens.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Environmental Sciences
Mon-Chien Lee, Yi-Ju Hsu, Yu-Qian Lin, Ling-Ni Chen, Mu-Tsung Chen, Chi-Chang Huang
Summary: Silver perch is a nutritious fish that offers various health benefits. Supplementation with Sante premium silver perch essence (SPSPE) for four weeks has been found to improve exercise performance, reduce sports fatigue, and promote fatigue recovery in mice. Moreover, it does not cause any physiological or histopathological damage.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Article
Biology
Chen-Chung Liao, Jin-Wei Xu, Wen-Ching Huang, Hung-Chang Chang, Yu-Tang Tung
Summary: Exercise can mitigate atherosclerosis by improving complement system activation and reducing inflammation in the aorta. This study found that exercise intervention had systematic effects on an ApoE knockout atherosclerotic model and high-fat-diet intervention, modulating multiple pathways and proteins associated with cardiovascular disease.
Article
Nutrition & Dietetics
Wen-Yang Lin, Yi-Wei Kuo, Jia-Hung Lin, Chi-Huei Lin, Jui-Fen Chen, Shin-Yu Tsai, Mon-Chien Lee, Yi-Ju Hsu, Chi-Chang Huang, Yung-An Tsou, Hsieh-Hsun Ho
Summary: This study tested the effectiveness of probiotic strains from different origins in weight loss and exercise performance enhancement, and found that OLP-01, PL-02, LY-66, TYCA06, and CS-773 were highly effective in both aspects.
Article
Plant Sciences
Ting-Yu Chen, Ya-Ling Chen, Wan-Chun Chiu, Chiu-Li Yeh, Yu-Tang Tung, Hitoshi Shirakawa, Wei-Tzu Liao, Suh-Ching Yang
Summary: The water extract of fermented rice bran (FRB) with higher antioxidant ability showed potential for preventing liver damage induced by a high-fat (HF) diet. This might be achieved through regulating imbalanced adipokines and maintaining a healthier microbiotic composition.
Article
Nutrition & Dietetics
Mon-Chien Lee, Ming-Ju Chen, Hsiao-Wen Huang, Wei-Kai Wu, Yi-Wei Lee, Hsing-Chun Kuo, Chi-Chang Huang
Summary: Exercise alters the gut microbiota, which in turn affects exercise performance. Supplementation with human-origin probiotics, such as Lactiplantibacillus plantarum Tana, can improve the gut microbiota, enhance exercise performance, and have antifatigue effects without causing any harm.
Article
Biochemistry & Molecular Biology
Hong-Jun Tai, Mon-Chien Lee, Yi-Ju Hsu, Chun-Yen Kuo, Chi-Chang Huang, Ming-Fu Wang
Summary: Sea bass, a fish rich in protein and omega-3, has been found to have many health benefits. This study investigated the effects of supplementing with Hi-Q sea bass essence on exercise performance and fatigue resistance. The results showed that sea bass essence supplementation significantly improved endurance exercise performance and glycogen storage in the liver and muscles. It also reduced fatigue-related biochemical markers. Overall, sea bass essence supplementation effectively improved exercise performance and had anti-fatigue effects.
Article
Nutrition & Dietetics
Mon-Chien Lee, Yi-Ju Hsu, Hsin-Ching Sung, Ya-Ting Wen, Li Wei, Chi-Chang Huang
Summary: Women during pregnancy and the postpartum period, especially those with excessive caloric intake, have a higher risk of obesity and metabolic diseases. This study found that mice with low-fitness phenotype were more likely to develop metabolic abnormalities and obesity after dietary induction following delivery, while mice with high-fitness phenotype had a protective mechanism.
Article
Biochemistry & Molecular Biology
Fat -Moon Suk, Fang-Yu Hsu, Yi-Chieh Lee, Tzu-Lang Chen, Wan-Chun Chiu, Yi-Jen Liao
Summary: Studies have found that oxidized dietary frying oil increases the risk of birth defects in mice and cancer and cardiovascular risks in humans. Furthermore, long-term consumption of fried foods can lead to liver damage and fibrosis by activating hepatic stellate cells, promoting inflammation, and inducing lipid peroxidation.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Nutrition & Dietetics
Yi-Ming Chen, Wan-Chun Chiu, Yen-Shuo Chiu
Summary: This study investigated the effect of Inonotus obliquus (IO) supplementation on body composition, energy expenditure, and exercise performance. The results showed that IO supplementation increased muscle volume, treadmill time, and glycogen storage in mice. It also improved serum free fatty acid levels after acute exercise, indicating changes in energy expenditure through the PPAR pathway. RNA sequencing revealed significant changes in metabolic pathways in the IO group compared to the vehicle group. This study only focused on IO supplementation and did not consider other training-related procedures.
Article
Polymer Science
Ming-Chi Hsieh, Ke-Chang Hung, Jin-Wei Xu, Yi-Hung Wu, Wen-Shao Chang, Jyh-Horng Wu
Summary: This study used near-infrared (NIR) spectroscopy and principal component analysis (PCA) to reveal the degradation of hemicellulose and amorphous cellulose region caused by vacuum hydrothermal (VH) treatment, leading to increased crystallinity and improved mechanical properties of luanta fir wood. The primary factors affecting these properties were found to be hemicellulose content, hydroxyl groups in the cellulose amorphous region, wood moisture content, and relative lignin content. The NIR technique can be a useful tool for predicting the mechanical properties of VH-treated wood.
Article
Pharmacology & Pharmacy
De-Shan Ning, Yu-Ju Chen, Chien-Ju Lin, Ching-Chiung Wang, Hong-Wei Zhao, Kun-Teng Wang, Ming-Chung Lee, Lemmuel L. Tayo, Wan-Chun Chiu, Chiu-Li Yeh, Chia-Jung Lee
Summary: This study used network pharmacology to predict the potential mechanisms of Livsooth authentic herbal formula (LAH) against non-alcoholic fatty liver disease (NAFLD) and validated the predictions in an animal model. The results showed that LAH improved hepatocyte steatosis in the liver tissue by activating the AMPK pathway and decreasing serum lipid and glucose levels. LAH also increased hepatic antioxidant enzyme activities, indicating an improvement in oxidative stress markers in NAFLD. In vitro experiments confirmed that the active component of LAH, puerarin, regulates lipid accumulation through the AMPK pathway.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Nutrition & Dietetics
Pei-Xin Huang, Chiu-Li Yeh, Suh-Ching Yang, Hitoshi Shirakawa, Chao-Lin Chang, Li-Hsin Chen, Yen-Shuo Chiu, Wan-Chun Chiu
Summary: This study investigated the effects of rice bran supplementation on gut microbiota and muscle mass in high-fat diet-fed mice. The results showed that rice bran improved gut barrier dysfunction and dysbiosis, and maintained muscle mass by regulating muscle atrophy-related factors.
Article
Polymer Science
Jin-Wei Xu, Cheng-Chun Li, Jian-Wei Liu, Wen-Chao Chang, Wen-Shao Chang, Jyh-Horng Wu
Summary: This study found that subjecting Japanese cedar wood to hydrothermal treatment at 180 degrees Celsius for 4 hours significantly improved its dimensional stability and creep resistance, while having little impact on its flexural properties.
Article
Multidisciplinary Sciences
Yu-Tang Tung, Ya-Ling Chen, Tzu-Yu Fan, Tsorng-Harn Fong, Wan-Chun Chiu
Summary: Estrogen deficiency can lead to obesity and metabolic syndrome, with fish oil and perilla oil playing a role in regulating inflammation and insulin signaling. Adjusting the dietary n-3:n-6 fatty-acid ratio to 1:2 may have beneficial effects on chronic diseases. Fish oil can alleviate insulin signaling through activating the PI3K-Akt-GLUT2 cascade signaling pathway.
Article
Nutrition & Dietetics
Dang Khanh Ngan Ho, Wan -Chun Chiu, Jing-Wen Kao, Hsiang -Tung Tseng, Chih-Yuan Yao, Hsiu-Yueh Su, Pin -Hui Wei, Nguyen Quoc Khanh Le, Hung Trong Nguyen, Jung -Su Chang
Summary: This study assessed the validity and sources of measurement errors of image-assisted mobile nutrition apps. Through data modification and cleaning, the accuracy of the Formosa FoodApp was significantly improved, indicating its validity and reliability as a self-reporting mobile-based dietary assessment tool.
