Article
Chemistry, Multidisciplinary
Sun Il Choi, Yu-Sun Lee, Yul Min Lee, Hyun Jung Kim, Won Jong Kim, Sungjin Jung, Ji Eun Im, Mi Rim Lee, Joon Ki Kim, A-Ra Jeon, Sang Myung Woo, Goo Taeg Oh, Kyun Heo, Yun-Hee Kim, In-Hoo Kim
Summary: In this study, a DNA aptamer, PAp7T8, was isolated using cell-SELEX technique to specifically target pancreatic cancer cells and tumors. By adopting a drug-conjugated oligobody strategy, PAp7T8-DOligobody was developed, which showed extended in vivo half-life and significantly inhibited tumor growth without causing significant toxicity. PAp7T8-DOligobody represents a promising novel therapeutic delivery platform for pancreatic ductal adenocarcinoma (PDAC).
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Chemistry, Multidisciplinary
Gang Yang, Shengnan Zhang, William Song, Xia Bai, Ling Li, Fatao Luo, Yiran Cheng, Diyue Wang, Yanqun Wang, Jiantao Chen, Jincun Zhao, Yongyun Zhao
Summary: A dual-function circular aptamer-ASO chimera (circSApt-NASO) is designed to suppress SARS-CoV-2 replication and inflammation, providing a potential therapy for COVID-19.
Article
Chemistry, Multidisciplinary
Liang Cui, Ruixiao Peng, Chaofei Zeng, Jialu Zhang, Yinzhu Lu, Lin Zhu, Mengjiao Huang, Qinghua Tian, Yanling Song, Chaoyong Yang
Summary: In this study, a general strategy for in situ detection of tumor-derived extracellular vesicle miRNAs was developed using aptamer-mediated selective fusion. This method offers high selectivity, rapidity, and general applicability, and enables sensitive and selective detection of tumor-derived EV miRNAs using conventional flow cytometry without the need for lysis.
Article
Pharmacology & Pharmacy
Stephanie He, Davindra Singh, Hossein Yusefi, Brandon Helfield
Summary: This study investigates the feasibility of delivering the angiogenesis-promoting miR-126 using ultrasound-stimulated microbubbles. The results show that by stimulating miR-126-decorated microbubbles, the intracellular levels of miR-126 can be increased while decreasing the expression of its target genes. This delivery method has the potential to stimulate therapeutic angiogenesis while minimizing endothelial damage.
Review
Chemistry, Multidisciplinary
Mengyuan Li, Lele Li
Summary: With the programmability of DNA, sensors can be developed for specific disease cells and spatially controlled molecular imaging. By combining DNA with enzymes, the limitations of DNA-based sensors can be overcome, allowing for improved cell specificity and signal-to-background ratio.
ACCOUNTS OF CHEMICAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Yi Li, Linyang Liu, Laicong Qiao, Fei Deng
Summary: CRISPR/Cas12a can be used for the development of a universal biosensing system for non-nucleic acid targets. By redirecting the DNA-cutting activity of Cas12a to specific DNA aptamers, a simple, sensitive, and universal biosensing platform called CAMERA has been established. CAMERA has advantages over traditional ELISA in terms of sensitivity, detection time, and thermal stability.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Sitao Xie, Weidi Sun, Ting Fu, Xiangsheng Liu, Peng Chen, Liping Qiu, Fengli Qu, Weihong Tan
Summary: Functional nucleic acid (NA)-based drugs have a wide range of applications in altering and controlling gene/protein expression patterns in cells. However, the precise delivery of functional NA to specific cells/organs is challenging due to the difficulty in crossing cell membranes and avoiding adverse effects on non-targeted cells. Aptamers, with their high specificity and selectivity for cell surface proteins, can be used as molecular recognition units for accurately binding target cells and enabling efficient cargo delivery. This perspective summarizes original aptamer-based strategies for targeted delivery of functional NA and discusses challenges and opportunities in this field.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Biophysics
Fatemeh Bakhshandeh, Sudip Saha, Payel Sen, Sadman Sakib, Roderick MacLachlan, Farhaan Kanji, Enas Osman, Leyla Soleymani
Summary: A signal-on biosensor was developed for ultrasensitive biosensing using nucleic acids to modulate photoelectrochemical (PEC) currents upon target capture. The assay showed a limit-of-detection of 82 pg/mL (13 pM) in buffer and 239 pg/mL (37 pM) in urine for peptidoglycan, as well as 1913 CFU/mL for Escherichia coli in urine. The versatility of the sensor was demonstrated by successfully identifying bacterial contamination versus fungi and detecting DNA targets with a limit-of-detection of 372 fM.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Multidisciplinary
Chen Zuo, Yongcan Guo, Junjie Li, Zhiping Peng, Shulian Bai, Shuangshuang Yang, Ding Wang, Hui Chen, Guoming Xie
Summary: The study introduces a new DNA-hybrid-gated core-shell upconversion nanoprobe for fluorescence analysis of microRNA-21, which can also be used for controlled drug delivery in cancer treatment. The nanoprobe shows good selectivity, a linear response towards miR-21, and a specific cytotoxic efficacy in tumor cells.
Article
Chemistry, Multidisciplinary
Ziwen Dai, Juan Li, Yongfang Lin, Zhigang Wang, Yang Huang
Summary: A functional drug delivery system was designed solely based on DNA with four DNA strands. Cyclization of DNA strands prevented the formation of byproducts. DNA aptamers were equipped to endow triangular DNA nanostructures with targeting ability. The homogeneity of materials enabled easy construction and convenient loading of nucleic acid-based drugs.
Review
Pathology
Abduladheem Turki Jalil, Mohanad Ali Abdulhadi, Lubna R. Al-Ameer, Hussein Abdullah Abbas, Muna. S. Merza, Rahman S. Zabibah, Ali A. Fadhil
Summary: MicroRNA-126 (miR-126) has diverse functions in cancer, affecting tumor angiogenesis, invasion, metastasis, cell proliferation, apoptosis, and treatment resistance. Dysregulation of miR-126 is associated with increased cancer risk and worse prognosis. Targeting miR-126 or its downstream effectors presents a potential therapeutic approach for inhibiting tumor development and combating drug resistance. Further investigation is needed to fully understand the mechanisms behind miR-126 dysregulation and develop efficient therapies.
PATHOLOGY RESEARCH AND PRACTICE
(2023)
Article
Polymer Science
Afsana Sheikh, Prashant Kesharwani
Summary: Aptamers are crucial in drug delivery but prone to degradation by endonucleases; bioconjugation with dendrimers offers a novel platform for improved targeted drug delivery system.
EUROPEAN POLYMER JOURNAL
(2021)
Article
Biochemical Research Methods
Xiaoying Zhu, Ying Ning, Zeshuai Zhang, Youqing Wen, Yuwen Zhao, Haixia Wang
Summary: An assembled-aptasensor based on Fe3O4@Au@Ag nanocomposites grafting onto the gold foil was prepared, which can be used for sensitive and rapid detection of various pathogenic bacteria. This sensor has good specific detection ability and can be used for rapid and sensitive detection of a variety of pathogens in complex substrates.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Ezaldeen Esawi, Walhan Alshaer, Ismail Sami Mahmoud, Dana A. Alqudah, Bilal Azab, Abdalla Awidi
Summary: The study developed a chimera consisting of two aptamers to selectively deliver doxorubicin into cancer cells and release the drug inside cells. Results demonstrate the selective uptake of the chimera into cancer cells, successful delivery of doxorubicin to targeted cells, inhibition of cancer cell growth, and reduced cytotoxicity on normal cells, indicating the potential of the AS1411-ATP aptamer chimera as an innovative drug delivery system for improved therapeutic efficacy with decreased off-target cytotoxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Cecil J. Weale, Don M. Matshazi, Saarah F. G. Davids, Shanel Raghubeer, Rajiv T. Erasmus, Andre P. Kengne, Glenda M. Davison, Tandi E. Matsha
Summary: This study found that miR-1299, miR-126-3p, and miR-30e-3p are associated with prediabetes, with miR-126-3p showing higher diagnostic capability for prediabetes.
Article
Biochemistry & Molecular Biology
Henrik Gustmann, Anna-Lena J. Segler, Dnyaneshwar B. Gophane, Andreas J. Reuss, Christian Gruenewald, Markus Braun, Julia E. Weigand, Snorri Th Sigurdsson, Josef Wachtveitl
NUCLEIC ACIDS RESEARCH
(2019)
Article
Biochemistry & Molecular Biology
Thilo Hetzke, Marc Vogel, Dnyaneshwar B. Gophane, Julia E. Weigand, Beatrix Suess, Snorri Th Sigurdsson, Thomas F. Prisner
Article
Biochemistry & Molecular Biology
Sandra Fischer, Antonella Di Liddo, Katarzyna Taylor, Jamina S. Gerhardus, Krzysztof Sobczak, Kathi Zarnack, Julia E. Weigand
Article
Biophysics
Sophie M. Korn, Karthikeyan Dhamotharan, Boris Fuertig, Martin Hengesbach, Frank Loehr, Nusrat S. Qureshi, Christian Richter, Krishna Saxena, Harald Schwalbe, Jan-Niklas Tants, Julia E. Weigand, Jens Woehnert, Andreas Schlundt
BIOMOLECULAR NMR ASSIGNMENTS
(2020)
Article
Biochemistry & Molecular Biology
Oliver Binas, Jan-Niklas Tants, Stephen A. Peter, Robert Janowski, Elena Davydova, Johannes Braun, Dierk Niessing, Harald Schwalbe, Julia E. Weigand, Andreas Schlundt
NUCLEIC ACIDS RESEARCH
(2020)
Article
Biophysics
F. Cantini, L. Banci, N. Altincekic, J. K. Bains, K. Dhamotharan, C. Fuks, B. Fuertig, S. L. Gande, B. Hargittay, M. Hengesbach, M. T. Hutchison, S. M. Korn, N. Kubatova, F. Kutz, V Linhard, F. Loehr, N. Meiser, D. J. Pyper, N. S. Qureshi, C. Richter, K. Saxena, A. Schlundt, H. Schwalbe, S. Sreeramulu, J-N Tants, A. Wacker, J. E. Weigand, J. Woehnert, A. C. Tsika, N. K. Fourkiotis, G. A. Spyroulias
BIOMOLECULAR NMR ASSIGNMENTS
(2020)
Article
Biophysics
Sophie M. Korn, Roderick Lambertz, Boris Fuertig, Martin Hengesbach, Frank Loehr, Christian Richter, Harald Schwalbe, Julia E. Weigand, Jens Woehnert, Andreas Schlundt
Summary: The international biomedical research community is working together to find promising viral drug targets to combat the COVID-19 pandemic caused by the novel coronavirus SARS-CoV-2. Scientists in the Covid19-NMR consortium are providing publicly available NMR data on viral proteins and RNAs to support drug development. The Nucleocapsid protein of SARS-CoV-2, with its conserved architecture comprising of N-terminal RNA-binding domain, SR-rich linker, and C-terminal dimerization domain, is a key focus for potential drug binding studies.
BIOMOLECULAR NMR ASSIGNMENTS
(2021)
Article
Biophysics
Robbin Schnieders, Stephen A. Peter, Elnaz Banijamali, Magdalena Riad, Nadide Altincekic, Jasleen Kaur Bains, Betul Ceylan, Boris Fuertig, J. Tassilo Gruen, Martin Hengesbach, Katharina F. Hohmann, Daniel Hymon, Bozana Knezic, Andreas Oxenfarth, Katja Petzold, Nusrat S. Qureshi, Christian Richter, Judith Schlagnitweit, Andreas Schlundt, Harald Schwalbe, Elke Stirnal, Alexey Sudakov, Jennifer Voegele, Anna Wacker, Julia E. Weigand, Julia Wirmer-Bartoschek, Jens Woehnert
Summary: The SARS-CoV-2 virus, responsible for the COVID-19 pandemic, contains an important sequence called SL5a in its genome, which is crucial for viral replication and could potentially be a target for antiviral drugs.
BIOMOLECULAR NMR ASSIGNMENTS
(2021)
Article
Biochemistry & Molecular Biology
Stephen A. Peter, Jessica S. Isaac, Franz Narberhaus, Julia E. Weigand
Summary: Proteome integrity is crucial for cellular homeostasis and adaptation to stress conditions like hypoxia, with alternative splicing being a mechanism for rapid adaptation. The isoE degron, generated under hypoxia, represents a universal protein degradation signal functional in mammalian cells, yeast, and bacteria. The conserved (F/W)xxW motif is essential for efficient protein decay mediated by the proteasome in eukaryotes and Lon protease in bacteria.
JOURNAL OF MOLECULAR BIOLOGY
(2021)
Correction
Biochemistry & Molecular Biology
Anna Wacker, Julia E. Weigand, Sabine R. Akabayov, Nadide Altincekic, Jasleen Kaur Bains, Elnaz Banijamali, Oliver Binas, Jesus Castillo-Martinez, Erhan Cetiner, Betuel Ceylan, Liang-Yuan Chiu, Jesse Davila-Calderon, Karthikeyan Dhamotharan, Elke Duchardt-Ferner, Jan Ferner, Lucio Frydman, Boris Fuertig, Jose Gallego, J. Tassilo Gruen, Carolin Hacker, Christina Haddad, Martin Haehnke, Martin Hengesbach, Fabian Hiller, Katharina F. Hohmann, Daniel Hymon, Vanessa de Jesus, Henry Jonker, Heiko Keller, Bozana Knezic, Tom Landgraf, Frank Loehr, Le Luo, Klara R. Mertinkus, Christina Muhs, Mihajlo Novakovic, Andreas Oxenfarth, Martina Palomino-Schaetzlein, Katja Petzold, Stephen A. Peter, Dennis J. Pyper, Nusrat S. Qureshi, Magdalena Riad, Christian Richter, Krishna Saxena, Tatjana Schamber, Tali Scherf, Judith Schlagnitweit, Andreas Schlundt, Robbin Schnieders, Harald Schwalbe, Alvaro Simba-Lahuasi, Sridhar Sreeramulu, Elke Stirnal, Alexey Sudakov, Jan-Niklas Tants, Blanton S. Tolbert, Jennifer Voegele, Lena Weiss, Julia Wirmer-Bartoschek, Maria A. Wirtz Martin, Jens Woehnert, Heidi Zetzsche
NUCLEIC ACIDS RESEARCH
(2021)
Editorial Material
Chemistry, Multidisciplinary
Elke Duchardt-Ferner, Jan Ferner, Boris Fuertig, Martin Hengesbach, Christian Richter, Andreas Schlundt, Sridhar Sreeramulu, Anna Wacker, Julia E. Weigand, Julia Wirmer-Bartoschek, Harald Schwalbe
Summary: The COVID-19 outbreak in December 2019 led to the establishment of international collaborations to understand and combat the virus. The NMR community has come together to study the genome and proteome of SARS-CoV-2 and test their interaction with low-molecular-weight binders. Due to the global pandemic and extended lockdowns, research has shifted from local collaborations to digital formats, allowing immediate discussion of unpublished results, data analysis, sample sharing, and coordinated research between more than 50 groups from 18 countries. Key lessons and challenges for post-pandemic research are discussed.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Sandra Fischer, Chiara Lichtenthaeler, Anastasiya Stepanenko, Florian Heyl, Daniel Maticzka, Katrin Kemmerer, Melina Klostermann, Rolf Backofen, Kathi Zarnack, Julia E. Weigand
Summary: HNRNPDL plays an important role in endothelial cell functions, and its knockdown affects cell proliferation, migration, and sprouting ability, possibly through regulating the expression of specific genes.
BIOLOGICAL CHEMISTRY
(2023)
Article
Biophysics
N. Kubatova, N. S. Qureshi, N. Altincekic, R. Abele, J. K. Bains, B. Ceylan, J. Ferner, C. Fuks, B. Hargittay, M. T. Hutchison, V. de Jesus, F. Kutz, M. A. Wirtz Martin, N. Meiser, V. Linhard, D. J. Pyper, S. Trucks, B. Furtig, M. Hengesbach, F. Lohr, C. Richter, K. Saxena, A. Schlundt, H. Schwalbe, S. Sreeramulu, A. Wacker, J. E. Weigand, J. Wirmer-Bartoschek, J. Woehnert
Summary: The international Covid19-NMR consortium aims to comprehensively characterize the spectroscopic properties of SARS-CoV-2's RNA elements and proteins, providing NMR chemical shift assignments for the virus's molecular components. This study reports near-complete NMR resonance assignments for the backbone chemical shifts of non-structural protein 10 (nsp10), which plays a vital role in viral replication.
BIOMOLECULAR NMR ASSIGNMENTS
(2021)
Article
Biochemistry & Molecular Biology
Anna Wacker, Julia E. Weigand, Sabine R. Akabayov, Nadide Altincekic, Jasleen Kaur Bains, Elnaz Banijamali, Oliver Binas, Jesus Castillo-Martinez, Erhan Cetiner, Betul Ceylan, Liang-Yuan Chiu, Jesse Davila-Calderon, Karthikeyan Dhamotharan, Elke Duchardt-Ferner, Jan Ferner, Lucio Frydman, Boris Fuertig, Jose Gallego, J. Tassilo Gruen, Carolin Hacker, Christina Haddad, Martin Haehnke, Martin Hengesbach, Fabian Hiller, Katharina F. Hohmann, Daniel Hymon, Vanessa de Jesus, Henry Jonker, Heiko Keller, Bozana Knezic, Tom Landgraf, Frank Loehr, Le Luo, Klara R. Mertinkus, Christina Muhs, Mihajlo Novakovic, Andreas Oxenfarth, Martina Palomino-Schatzlein, Katja Petzold, Stephen A. Peter, Dennis J. Pyper, Nusrat S. Qureshi, Magdalena Riad, Christian Richter, Krishna Saxena, Tatjana Schamber, Tali Scherf, Judith Schlagnitweit, Andreas Schlundt, Robbin Schnieders, Harald Schwalbe, Alvaro Simba-Lahuasi, Sridhar Sreeramulu, Elke Stirnal, Alexey Sudakov, Jan-Niklas Tants, Blanton S. Tolbert, Jennifer Voegele, Lena Weiss, Julia Wirmer-Bartoschek, Maria A. Wirtz Martin, Jens Wohnert, Heidi Zetzsche
NUCLEIC ACIDS RESEARCH
(2020)
Article
Cell Biology
Antonella Di Liddo, Camila de Oliveira Freitas Machado, Sandra Fischer, Stefanie Ebersberger, Andreas W. Heumueller, Julia E. Weigand, Michaela Mueller-McNicoll, Kathi Zarnack
JOURNAL OF MOLECULAR CELL BIOLOGY
(2019)