期刊
REVIEW OF SCIENTIFIC INSTRUMENTS
卷 85, 期 10, 页码 -出版社
AMER INST PHYSICS
DOI: 10.1063/1.4898019
关键词
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资金
- Academia Sinica, National Science Council of Taiwan [NSC99-2112-M-001-029MY3, NSC98-2120-M-001-007-CC2, NSC102-2112-M-001024-MY3, NSC103-2917-I-564-072]
- Ministry of Science and Technology [MOST103-2627-M-001-011]
High-speed atomic force microscopy (HS-AFM) enables visualizing dynamic behaviors of biological molecules under physiological conditions at a temporal resolution of 1s or shorter. A small cantilever with a high resonance frequency is crucial in increasing the scan speed. However, detecting mechanical resonances of small cantilevers is technically challenging. In this study, we constructed an atomic force microscope using a digital versatile disc (DVD) pickup head to detect cantilever deflections. In addition, a flexure-guided scanner and a sinusoidal scan method were implemented. In this work, we imaged a grating sample in air by using a regular cantilever and a small cantilever with a resonance frequency of 5.5 MHz. Poor tracking was seen at the scan rate of 50 line/s when a cantilever for regular AFM imaging was used. Using a small cantilever at the scan rate of 100 line/s revealed no significant degradation in the topographic images. The results indicate that a smaller cantilever can achieve a higher scan rate and superior force sensitivity. This work shows the potential for using a DVD pickup head in future HS-AFM technology. (C) 2014 AIP Publishing LLC.
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