期刊
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
卷 185, 期 3, 页码 571-581出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resp.2012.11.009
关键词
Hypoxia; Ventilatory responses; S-nitrosoglutathione reductase; S-nitrosothiols; Mice
资金
- NIH [1P01HL101871, R01 HL59337]
- Department of Defense [W81XWH-07-0134]
- Galleon Pharmaceuticals
Exposure to a hypoxic challenge increases ventilation in wild-type (WT) mice that diminish during the challenge (roll-off) whereas return to room air causes an increase in ventilation (short-term facilitation, STF). Since plasma and tissue levels of ventilatory excitant S-nitrosothiols such as S-nitrosoglutathione (GSNO) increase during hypoxia, this study examined whether (1) the initial increase in ventilation is due to generation of GSNO, (2) roll-off is due to increased activity of the GSNO degrading enzyme, GSNO reductase (GSNOR), and (3) STF is limited by GSNOR activity. Initial ventilatory responses to hypoxic challenge (10% O-2, 90% N-2) were similar in WT, GSNO+/- and GSNO-/- mice. These responses diminished markedly during hypoxic challenge in WT mice whereas there was minimal roll-off in GSNOR+/- and GSNOR-/- mice. Finally, STF was greater in GSNOR+/- and GSNOR-/- mice than in WT mice (especially females). This study suggests that GSNOR degradation of GSNO is a vital step in the expression of ventilatory roll-off and that GSNOR suppresses STF. (C) 2012 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据