Article
Critical Care Medicine
Liangfeng Mao, Maomao Sun, Zhenfeng Chen, Zhenhua Zeng, Jie Wu, Zhongqing Chen, Weijin Zhang, Qiaobing Huang
Summary: In this study, the researchers found that activation of PDHC can reverse the increased glycolysis level, reduce lactate production, and prevent endothelial barrier dysfunction induced by LPS. Additionally, LPS treatment increased the phosphorylation and acetylation of PDH E1α.
Article
Endocrinology & Metabolism
Jili Wen, Pengwei Zhao, HuiJuan Ren, JunMin Wu
Summary: The study found that lipopolysaccharides (LPS) can promote autophagy in human pulmonary microvascular endothelial cells (HPMECs) by inhibiting the expression of TIMAP and activating moesin, which in turn inhibits the PI3K/Akt/mTOR signaling pathway. This discovery reveals the protective role of the LPS-TIMAP-moesin-PI3K/Akt/mTOR axis in autophagy.
JOURNAL OF BIOLOGICAL REGULATORS AND HOMEOSTATIC AGENTS
(2023)
Article
Multidisciplinary Sciences
Junling Yang, Yinchuan Li, Ambuj Bhalla, Mark Maienschein-Cline, Ken-ichiro Fukuchi
Summary: To study the effects of macrophage-derived inflammatory mediators on brain endothelial cells, a co-culture system of bEnd.3 cells and LPS-activated Raw 264.7 cells was established and cytokine profiling was performed. The results showed that the co-culture model had similar upregulation of eight cytokines/chemokines compared to the in vivo mouse model. In contrast, the mono-culture system of LPS-activated bEnd.3 cells had little similarity to the in vivo model. This co-culture model provides valuable insights into the molecular mechanisms of neuroinflammation induced by systemic inflammation.
Article
Biochemistry & Molecular Biology
Wesuk Kang, Bomin Son, Soyoon Park, Dabin Choi, Taesun Park
Summary: This study demonstrates that ORs are highly expressed in HaCaT cells, especially under UV or inflammation exposure. Activation of specific ORs by odorant ligands can downregulate skin barrier genes in HaCaT cells, indicating the potential of ORs as markers for skin barrier abnormalities and the need for further research on their role in barrier dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Ikhyun Jun, Young Joon Choi, Bo-Rahm Kim, Kyoung Yul Seo, Tae-im Kim
Summary: This study discovered that activation of the ADRB2/PKA signaling pathway enhances lipid synthesis in human meibomian gland epithelial cells (HMGECs), providing a potential mechanism and therapeutic target for meibomian gland dysfunction.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xiujun Lin, Yang Li, Baokun Qi, Shuang Zhang, Xue Li
Summary: This study found that caseinphosphatidylcholine (CP) has good biosafety in intestinal barrier and can repair LPS-induced intestinal barrier dysfunction by regulating ferroptosis and lipid metabolism. These findings provide new insights for the design of new healthy emulsifiers.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Multidisciplinary Sciences
Chen-Xiang Wei, Ju-Hua Wu, Yue-Hong Huang, Xiao-Zhong Wang, Jian-Ying Li
Summary: The study demonstrated that LP can improve the damage of LPS-induced Caco2 cell membrane barrier function by regulating the cAMP-PKA signaling pathway, increasing the expression of TJ proteins and electrical resistance detection between cells.
Article
Chemistry, Medicinal
Khadeja-Tul Kubra, Mohammad A. Uddin, Nektarios Barabutis
Summary: In this study, we found that the use of UPR inducer Tunicamycin can protect against injury caused by lipopolysaccharide. By modulating actomyosin remodeling and enhancing endothelial integrity, TM supports vascular barrier function. Our findings suggest that UPR activation may provide novel therapeutic opportunities for diseases related to endothelial dysregulation.
Article
Chemistry, Analytical
Yi Xu, Shuainan Li, Chenguang Wang, Xiaoming Xie, Xianqiang Mi
Summary: The blood-brain barrier (BBB) is crucial for maintaining CNS stability, and neuroinflammation can disrupt its function. MiR-146a is closely associated with neuroinflammation and its expression is significantly upregulated after LPS induction. Studying miR-146a expression in the BBB can provide insights into the mechanisms of neurological diseases.
ANALYTICAL CHEMISTRY
(2023)
Article
Surgery
Molly Kobritz, Colleen Nofi, Maria Sfakianos, Gene Coppa, Monowar Aziz, Ping Wang
Summary: H151 has the potential to therapeutically reduce acute intestinal injury in sepsis.
Review
Pharmacology & Pharmacy
Geetika Nehra, Bjoern Bauer, Anika M. S. Hartz
Summary: This review summarizes the extent and clinical relevance of barrier leakage in Alzheimer's disease (AD). By examining animal models, clinical and postmortem studies, signaling mechanisms, and potential therapeutic strategies, the review provides insights into the relationship between barrier leakage and neurodegeneration, cognitive decline, and the development of novel therapeutic targets for AD.
PHARMACOLOGY & THERAPEUTICS
(2022)
Article
Biotechnology & Applied Microbiology
Yuehong Pu, Lei Zhao, Yao Xi, Yichun Xia, Yiming Qian
Summary: The study evaluated the therapeutic effects of Mirtazapine, a central presynaptic alpha 2 receptor antagonist, on LPS-induced blood-brain barrier disruption. The results showed that Mirtazapine could protect the blood-brain barrier from LPS-induced damage and reverse the inflammatory response.
Article
Biochemistry & Molecular Biology
Ambika Verma, Gohar Azhar, Xiaomin Zhang, Pankaj Patyal, Grishma Kc, Shakshi Sharma, Yingni Che, Jeanne Y. Wei
Summary: This study investigated the role of Porphyromonas gingivalis (P. gingivalis)-lipopolysaccharide (LPS) in mitochondrial dysfunction during neurodegeneration. The results showed that LPS treatment activated toll-like receptor 4 signaling and upregulated the expression of Alzheimer's disease-related dementia and neuroinflammatory markers. LPS treatment also increased reactive oxygen species production and decreased mitochondrial membrane potential. Furthermore, LPS treatment repressed genes involved in mitochondrial function and biogenesis, specifically affecting complexes I, II, and IV of the mitochondrial electron transport chain.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Arthid Thim-Uam, Jiradej Makjaroen, Jiraphorn Issara-Amphorn, Wilasinee Saisorn, Dhammika Leshan Wannigama, Wiwat Chancharoenthana, Asada Leelahavanichkul
Summary: Splenectomy may lead to gut barrier defect and dysbiosis, increasing susceptibility to systemic infection. Splenectomy mice showed more severe mucositis in DSS-induced colitis and lower serum cytokines in LPS injection models. The spleen plays a crucial role in controlling bacterial translocation from the gut. LPS tolerance was observed in splenectomy mice and resulted in reduced cell activities in macrophages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Seunghee Lee, Byeong Geun Seok, Seon-Jin Lee, Su Wol Chung
Summary: This study investigated the mechanism and function of mitoNEET during LPS-induced inflammatory responses. The results suggest that inhibition of mitoNEET reduces the levels of pro-inflammatory cytokines, decreases ROS formation and mitochondrial dysfunction, providing a new approach for treating inflammatory diseases and sepsis.
CELL DEATH & DISEASE
(2022)
Article
Cell Biology
Robert Batori, Sanjiv Kumar, Zsuzsanna Bordan, Mary Cherian-Shaw, Anita Kovacs-Kasa, Justin A. MacDonald, David J. R. Fulton, Ferenc Erdodi, Alexander D. Verin
JOURNAL OF CELLULAR PHYSIOLOGY
(2019)
Article
Pharmacology & Pharmacy
Lina Wang, Yapeng Cao, B. Gorshkov, Yaqi Zhou, Qiuhua Yang, Jiean Xu, Qian Ma, Xiaoyu Zhang, Jingjing Wang, Xiaoxiao Mao, Xianqiu Zeng, Yunchao Su, A. D. Verin, Mei Hong, Zhiping Liu, Yuqing Huo
PHARMACOLOGICAL RESEARCH
(2019)
Letter
Critical Care Medicine
Alexander D. Verin
Review
Biochemistry & Molecular Biology
N. V. Bogatcheva, M. E. Coleman
BIOCHEMISTRY-MOSCOW
(2019)
Article
Cell Biology
Alexander D. Verin, Robert Batori, Anita Kovacs-Kasa, Mary Cherian-Shaw, Sanjiv Kumar, Istvan Czikora, Vijaya Karoor, Derek Strassheim, Kurt R. Stenmark, Evgenia Gerasimovskaya
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Mobarak Abu Mraheil, Haroldo A. Toque, Luigi La Pietra, Juerg Hamacher, Tenzing Phanthok, Alexander Verin, Joyce Gonzales, Yunchao Su, David Fulton, Douglas C. Eaton, Trinad Chakraborty, Rudolf Lucas
Summary: Pneumococci promote colonization by producing high levels of H2O2 and have unique mechanisms to protect themselves from its damage. The production of H2O2 forms an important virulence phenotype and overlaps with the activities of other virulence factors.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Article
Cell Biology
Anita Kovacs-Kasa, Laszlo Kovacs, Mary Cherian-Shaw, Vijay Patel, Mary L. Meadows, David J. Fulton, Yunchao Su, Alexander D. Verin
Summary: TMP269, a specific Class IIa HDACs inhibitor, attenuates LPS-induced ALI by decreasing myosin light chain phosphorylation and improving vascular barrier integrity. This suggests a specific mechanism by which Class IIa HDACs regulate endothelial permeability in response to LPS-induced injury.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Multidisciplinary Sciences
Christine M. Gross, Anita Kovacs-Kasa, Mary Louise Meadows, Mary Cherian-Shaw, David J. Fulton, Alexander D. Verin
SCIENTIFIC REPORTS
(2020)
Article
Cell Biology
Natalia V. Bogatcheva, Michael E. Coleman
Summary: ASC-CS shows anti-inflammatory effects in mice infected with pneumonia-like influenza A virus, limiting lung injury and reducing protein leakage and inflammatory cell infiltration. Pre-treatment of ASC enhances the therapeutic effects of ASC-CS, particularly with priming with inflammatory factors.
Article
Biochemistry & Molecular Biology
Yunchao Su, Weihong Han, Anita Kovacs-Kasa, Alexander D. Verin, Laszlo Kovacs
Summary: The study shows that HDAC6 expression is upregulated in patients with COPD and in a COPD animal model. Cigarette smoke extract, PDGF, and IL-6 increase protein levels and activation of HDAC6 in bronchial and pulmonary smooth muscle cells, leading to collagen synthesis and cell proliferation. Pharmacological inhibition of HDAC6 prevents airway and vascular remodeling in COPD.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Vijaya Karoor, Derek Strassheim, Timothy Sullivan, Alexander Verin, Nagavedi S. Umapathy, Edward C. Dempsey, Daniel N. Frank, Kurt R. Stenmark, Evgenia Gerasimovskaya
Summary: Butyrate shows protective effects in a rat model of hypoxic pulmonary hypertension by preventing pulmonary vascular remodeling and increased permeability, reducing right ventricular hypertrophy, and increasing histone H3 acetylation. Additionally, it modulates cytokine profiles in lung tissue and upregulates tight junctional proteins in endothelial cells, suggesting its potential as a complementary treatment for PH and other cardiovascular diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Physiology
Rudolf Lucas, Yalda Hadizamani, Perenlei Enkhbaatar, Gabor Csanyi, Robert W. Caldwell, Harald Hundsberger, Supriya Sridhar, Alice Ann Lever, Martina Hudel, Dipankar Ash, Masuko Ushio-Fukai, Tohru Fukai, Trinad Chakraborty, Alexander Verin, Douglas C. Eaton, Maritza Romero, Juerg Hamacher
Summary: Alveolar-capillary leak is a hallmark of ARDS, which is a potentially lethal complication of severe sepsis, trauma, and pneumonia (including COVID-19). ARDS is characterized by barrier dysfunction, hyper-inflammation, and impaired alveolar fluid clearance, leading to pulmonary permeability edema and compromised gas exchange.
FRONTIERS IN PHYSIOLOGY
(2022)
Editorial Material
Physiology
Narasimham Parinandi, Evgenia Gerasimovskaya, Alexander Verin
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Immunology
Robert K. Batori, Feng Chen, Zsuzsanna Bordan, Stephen Haigh, Yunchao Su, Alexander D. Verin, Scott A. Barman, David W. Stepp, Trinad Chakraborty, Rudolf Lucas, David J. R. Fulton
Summary: This study found that Cav-1 protects the endothelial barrier against PLY-induced disruption. Depletion of cholesterol in the plasma membrane increases intracellular calcium levels induced by PLY, but the presence of Cav-1 in the membrane can reduce this effect. Furthermore, Cav-1 promotes endocytosis of damaged membrane, reducing the invasion of PLY.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Qian Ma, Qiuhua Yang, Jiean Xu, Hunter G. Sellers, Zach L. Brown, Zhiping Liu, Zsuzsanna Bordan, Xiaofan Shi, Dingwei Zhao, Yongfeng Cai, Vidhi Pareek, Chunxiang Zhang, Guangyu Wu, Zheng Dong, Alexander D. Verin, Lin Gan, Quansheng Du, Stephen J. Benkovic, Suowen Xu, John M. Asara, Issam Ben-Sahra, Scott Barman, Yunchao Su, David J. R. Fulton, Yuqing Huo
Summary: This study found that the enzyme ATIC, which mediates de novo purine synthesis, plays a critical role in the proliferation of pulmonary artery smooth muscle cells (PASMCs) and the development of pulmonary hypertension (PH). Targeting ATIC-mediated DNPS reduces the availability of purine nucleotides, leading to decreased PASMC proliferation and amelioration of PH development and progression.
EUROPEAN HEART JOURNAL
(2023)
Article
Physiology
Alyssa R. Mickle, Jesus D. Penaloza-Aponte, Richard Coffey, Natale A. Hall, David Baekey, Erica A. Dale
Summary: Closed-loop epidural stimulation (CL-ES) can improve respiratory deficits caused by cervical spinal cord injury, restoring diaphragm activity and enhancing contralateral activity. This treatment has the potential to lead to lasting recovery and device independence.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Shawn Miller Jr, Edgar Juarez Lopez, Jessica M. L. Grittner, Brendan J. Dougherty
Summary: This study tested the impact of CO2 supplementation at different concentrations on ventilatory long-term facilitation (vLTF) in rats and found that 2% CO2 supplementation during and after acute, intermittent hypoxia (AIH) was sufficient to maintain isocapnia and induce significant vLTF.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Behnam Vafadari, Yoshitaka Oku, Charlotte Tacke, Ali Harb, Swen Huelsmann
Summary: The preBo·tzinger Complex (preBo·tC) in the brainstem plays a critical role in generating respiratory rhythm. This study aimed to investigate the activity of inhibitory glycinergic neurons in the preBo·tC of anesthetized mice. Using juxtacellular recordings and optogenetic activation, the researchers were able to identify and characterize the activity pattern of these inhibitory neurons in relation to the breathing rhythm.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Stephen M. Johnson, Maia G. Gumnit, Sarah M. Johnson, Tracy L. Baker, Jyoti J. Watters
Summary: Low-level activation of mu-opioid receptors can increase the amplitude of inspiratory bursts in neonatal rat brainstem-spinal cord preparations. Endomorphin-2, an endogenous ligand for these receptors, was found to have similar effects. Disinhibition of inhibitory synaptic transmission may not be involved in the changes induced by endomorphin-2, and different mechanisms may underlie the increase in burst amplitude and decrease in burst frequency.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Raphael Martins de Abreu, Beatrice Cairo, Patricia Rehder-Santos, Claudio Donisete da Silva, Etore De Favari Signini, Juliana Cristina Milan-Mattos, Camila Akemi Sakaguchi, Aparecida Maria Catai, Alberto Porta
Summary: The purpose of this study was to determine the association between exercise capacity based on peak oxygen uptake (VO2peak) and resting cardiorespiratory coupling (CRC) levels. The results showed that resting CRC values were associated with exercise capacity in athletes but not in non-athletes.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Sarah M. Russel, Raluca E. Gosman, Katherine Gonzalez, Joshua Wright, Dennis O. Frank-Ito
Summary: This study aimed to characterize the response of different nasal morphologies to airflow-related variables during rapid/deep inspiration. The results showed that notched nasal vestibules had higher resistance values and airflow velocities, while standard nasal vestibules had higher mucosal heat flux. Different nasal phenotypes may predispose individuals to exercise-induced rhinitis.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Ivan Poliacek, Lukas Martvon, Michal Simera, Marcel Veternik, Jakub Misek, Lucia Cibulkova, Kimberly E. Iceman, Donald C. Bolser, Teresa Pitts
Summary: An animal model study evaluated the effects of an abdominal incision on cough and swallow, revealing that abdominal wall manipulations can increase pharyngeal muscle activity during swallow without affecting the cough reflex. Swallowing tended to occur more during the inspiratory phase. The results highlight the important role of abdominal wall sensory feedback in regulating swallow motor patterns.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Rui Yu, Tatsuma Okazaki, Yuzhuo Ren, Junko Okuyama, Satoru Ebihara, Shin-Ichi Izumi
Summary: Body postures significantly influence respiratory muscle force, cough pressure, subjective ease of coughing, and pulmonary function. The 60-degree semi-recumbent posture and sitting posture show better results compared to the supine posture.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Suzuna Sato, Koji Ishida, Noriko I. Tanaka, Keisho Katayama
Summary: Respiratory muscle endurance training has beneficial effects on whole-body endurance performance. A novel high-intensity interval (HII) protocol is found to enhance the efficacy of respiratory muscle training programs.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Letter
Physiology
R. Arieli
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Correction
Physiology
Ivan Poliacek, Michal Simera, Marcel Veternik, Zuzana Kotmanova, Teresa Pitts, Jan Hanacek, Jana Plevkova, Peter Machac, Nadezda Visnovcova, Jakub Misek, Jan Jakus
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)
Article
Physiology
Dimitrios I. Bourdas, Nickos D. Geladas
Summary: This study examined the effects of breath-hold training on the performance of novice and elite breath-hold divers. The results showed that breath-hold training significantly improved red blood cell concentration, hemoglobin oxygen saturation steady state duration, and breath-hold time in novice divers. Elite divers had better breath-hold performance and higher peak mean arterial pressure compared to novices. The study suggests that breath-hold training can enhance the performance of divers.
RESPIRATORY PHYSIOLOGY & NEUROBIOLOGY
(2024)