期刊
RESEARCH IN VETERINARY SCIENCE
卷 97, 期 1, 页码 43-45出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2014.05.010
关键词
Toll-like receptor; Duck; Molecular cloning; Expression; Flagellin; Function
资金
- National Natural Science Foundation of China [31320103907, 31372415, 31230070, 31172299]
- Program for New Century Excellent Talents in University [NCET-12-0745]
- Six Talent Peaks Program of Jiangsu Province [NY-028]
- Qinglan Program of Jiangsu Province
- Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
Toll-like receptor 5 (TLR5) is responsible for the recognition of bacterial flagellin in vertebrates. In this study, we cloned the single-exon TLR5 gene of the Maya breed of Common Shelduck (Tadorna tadorna). The TLR5 open reading frame is 2580 bp in length and encodes an 859-amino acid protein. The putative amino acid sequence of duck TLR5 consisted of a signal peptide sequence, 11 leucine-rich repeat domains, a leucine-rich repeat C-terminal domain, a transmembrane domain, and an intracellular Toll-interleukin-1 receptor domain. The duck TLR5 gene was highly expressed in the lung, bone marrow, spleen, and liver; moderately expressed in kidney, small intestine, large intestine, and brain. A plasmid expressing duck TLR5 was constructed and transfected into HEK293T cells, and expression was confirmed by indirect immunofluorescence assay. HEK293T cells transfected with duck TLR5- and NF-kappa B-luciferase-containing plasmids significantly responded to flagellin from Salmonella typhimurium, indicating that it is a functional TLR5 homolog. (C) 2014 Elsevier Ltd. All rights reserved.
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