Antiproliferative effect of α-mangostin on canine osteosarcoma cells

Osteosarcoma is the most frequently diagnosed primary bone tumor in dog. Since chemotherapeutics are quite limited due to high cost and severe toxicity, therefore, the ultimate goal is to discover cost-effective therapeutics with less toxicity. We have studied the effect of alpha-mangostin, a xanthone derivative isolated from pericarp of mangosteen (Garcinia mangostana Linn.) in canine osteosarcoma, D-17 cells. The results showed that a-mangostin induced antiproliferation with IC50 at 15 mu g/ml. Hoechst 33342 nuclear staining and nucleosomal DNA-gel electrophoresis revealed that alpha-mangostin could induce nuclear condensation and fragmentation, typically seen in apoptosis. Cell cycle analysis demonstrated that a-mangostin induced sub-G1 peak. In addition, alpha-mangostin also induced membrane flipping of the phosphatidylserine and the loss of mitochondrial membrane potential in D-17 cells. In conclusion, a-mangostin, induced apoptotic cell death against canine osteosarcoma D-17 cells, could be a potential candidate for preventive and therapeutic application for bone cancer treatment in dogs. (c) 2012 Elsevier Ltd. All rights reserved.