期刊
RESEARCH IN VETERINARY SCIENCE
卷 91, 期 2, 页码 254-260出版社
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2010.11.016
关键词
Cancer stem cell; Sphere; Dog; Mammary tumor; Mammary gland
资金
- Japan Society for the Promotion of Science [19780235, 21780293]
- Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT)
- Grants-in-Aid for Scientific Research [19780235, 21780293] Funding Source: KAKEN
There is increasing evidence for the presence of cancer stem cells in several solid tumors, and these cancer stem cells have a potential role in tumor initiation, aggression, and recurrence. The stem cell-like properties of spheres derived from canine mammary tumors remain largely elusive. We attempted to induce sphere formation using four cell lines of canine mammary adenocarcinoma, and characterized the spheres derived from a CHMp line in vitro and in vivo. The CHMp-derived spheres showed predominantly CD44(+)CD24(-) population, higher expression of stem cell-related genes, such as CD133, Notch3 and MDR, and higher resistance to doxorubicin compared with the CHMp-derived adherent cells. Xenograft transplantations in nude mice demonstrated that only 1 x 10(4) sphere cells were sufficient for tumor formation. Use of the sphere assay on these sphere-derived tumors showed that sphere-forming cells were present in the tumors, and were maintained in serial transplantation. We propose that spheres derived from canine mammary adenocarcinoma cell lines possess a potential characteristic of cancer stem cells. Spheres derived from canine mammary tumors could be a powerful tool with which to investigate novel therapeutic drugs and to elucidate the molecular and cellular mechanisms that underlie tumorigenesis. (C) 2010 Elsevier Ltd. All rights reserved.
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