Article
Multidisciplinary Sciences
Amal F. F. Soliman, Mohamed A. A. Sabry, Gehad Abdelwahab
Summary: The essential oil isolated from Araucaria heterophylla resin was analyzed, and 24 components were identified, accounting for 99.89% of the total detected constituents. The oil exhibited inhibitory activity against aldose reductase and BuCHE enzymes, possibly due to compounds containing epoxide and hydroxyl groups. This study provides preliminary screening for the oil as potential therapeutic agents for antidiabetic cataract and Alzheimer's disease.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Muhammad Taha, Fazal Rahim, Nizam Uddin, Ihsan Ullah Khan, Naveed Iqbal, El Hassane Anouar, Mohammed Salahuddin, Rai Khalid Farooq, Mohammed Gollapalli, Khalid Mohammed Khan, Ameeduzzafar Zafar
Summary: Indole-based thiadiazole derivatives were synthesized and evaluated for their inhibition of AChE and BChE. Compounds with para, ortho, and meta-fluoro substitutions on the phenyl ring attached to thiadiazole showed the highest activity. Characterization was done using NMR, MS, and molecular docking studies were conducted.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Food Science & Technology
Rares Birsan, Peter Wilde, Keith W. Waldron, Dilip K. Rai
Summary: The study indicates that polyphenol-rich brewer's spent grain (BSG) fractions have the potential to act as natural anti-cholinesterase agents. The diethyl ether fraction of the saponified extract showed the best inhibitions of cholinesterases.
Review
Biochemistry & Molecular Biology
Anna Bubley, Alexaner Erofeev, Peter Gorelkin, Elena Beloglazkina, Alexander Majouga, Olga Krasnovskaya
Summary: Alzheimer's disease (AD) is a neurodegenerative disorder characterized by Aβ aggregation, tau hyperphosphorylation, and loss of cholinergic neurons. AD is also associated with oxidative stress, metal dyshomeostasis, inflammation, and cell cycle dysregulation. In this review, THA-based hybrids are summarized as potential anti-AD agents, highlighting strategies for drug design that have led to cognitive improvements and reduced hepatotoxicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Ginson George, Vishal Payyalot Koyiparambath, Sunitha Sukumaran, Aathira Sujathan Nair, Leena K. Pappachan, Abdullah G. Al-Sehemi, Hoon Kim, Bijo Mathew
Summary: This review highlights the recent evidence of chalcones as a privileged structure in the development of drugs for Alzheimer's disease. Different classes of chalcone-derived analogs and the importance of certain functionalities in exhibiting pharmaceutical activity are summarized.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Hyun Myung Lee, Rudolf Andrys, Jakub Jonczyk, Kyuneun Kim, Avinash G. Vishakantegowda, David Malinak, Adam Skarka, Monika Schmidt, Michaela Vaskova, Kamil Latka, Marek Bajda, Young-Sik Jung, Barbara Malawska, Kamil Musilek
Summary: The designed and synthesised pyridinium-2-carbaldoximes with quinolinium carboxamide moiety showed intermediate-to-high inhibition of both cholinesterases compared to standard oximes. In vitro evaluation revealed their reactivation ability on human recombinant acetylcholinesterase (hrAChE) and human recombinant butyrylcholinesterase (hrBChE), with one compound showing reactivation of all used organophosphates on hrAChE, and two novel compounds able to reactivate NIMP/NEMP-hrBChE. The molecular modelling results highlighted the importance of creating a pre-reactivation complex for better reactivation of both cholinesterases.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2021)
Article
Chemistry, Medicinal
Raquel B. M. de Almeida, Deyse B. B. Barbosa, Mayra R. R. do Bomfim, Jessika A. O. Amparo, Bruno S. S. Andrade, Silvia L. L. Costa, Joaquin M. Campos, Jorddy N. Cruz, Cleydson B. R. Santos, Franco H. A. Leite, Mariana B. B. Botura
Summary: The compound ZINC390718 was found to exhibit inhibitory activity against acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE), and showed low cytotoxicity in vitro. Molecular dynamics (MD) simulation revealed that ZINC390718 interacted with the catalytic residue sites of both enzymes. These findings suggest that ZINC390718 could be a potential chemotype for the development of new dual cholinesterase inhibitors.
Article
Chemistry, Medicinal
Tuan Xu, Shuaizhang Li, Andrew J. Li, Jinghua Zhao, Srilatha Sakamuru, Wenwei Huang, Menghang Xia, Ruili Huang
Summary: In this study, machine learning models were developed to predict novel AChE and BChE inhibitors. The models showed good performance in virtual screening and increased the hit rate of assay. A total of 88 novel AChE and 126 novel BChE inhibitors were identified, with significant inhibitory effects. Structure-activity relationship analysis revealed scaffolds for chemistry design and optimization.
JOURNAL OF CHEMICAL INFORMATION AND MODELING
(2023)
Article
Toxicology
Edward C. Meek, Russell L. Carr, Janice E. Chambers
Summary: The mechanism of toxic action for organophosphates involves persistent inhibition of acetylcholinesterase, leading to hyperstimulation of the nervous system. Differences in their chemistries result in varied metabolism and toxicity. Age-related differences in sensitivity to organophosphates in mammals are primarily influenced by detoxication capacities.
TOXICOLOGY IN VITRO
(2021)
Article
Biochemical Research Methods
Renato Simoes Moreira, Nathalia Anderson Calomeno, Gabriella Bassi das Neves, Luiz Flavio Nepomuceno do Nascimento, Vilmar Benetti Filho, Glauber Wagner, Luiz Claudio Miletti
Summary: This study aims to analyze in vitro secreted proteins from T. evansi and identify potential treatment and diagnostic biomarkers for surra diagnosis.
JOURNAL OF PROTEOMICS
(2023)
Article
Chemistry, Multidisciplinary
Saira Naseem, Ahmed Temirak, Aqeel Imran, Saquib Jalil, Shamool Fatima, Parham Taslimi, Jamshed Iqbal, Mussarat Tasleem, Muhammad Nawaz Tahir, Zahid Shafiq
Summary: In this study, new 1,3,4-oxadiazole derivatives were synthesized and tested as novel inhibitors of monoamine oxidase and cholinesterase enzymes. Several compounds showed promising inhibitory effects on these enzymes, with some compounds displaying multitargeting activity. These newly synthesized analogues represent potential lead compounds for the development of neurological disease treatments.
Article
Chemistry, Medicinal
Suat Sari, Seda Onder, Didem Akkaya, Suna Sabuncuoglu, Merve Zengin, Burak Barut, Arzu Karakurt
Summary: This study examined the inhibitory effects of a class of compounds called Azoles on AChE and BChE, and discovered two derivatives with strong inhibition against both wildtype and mutant BChE. Kinetic analysis and molecular modeling further supported these findings and provided insights into the inhibitory behavior of these compounds against mutant BChE forms.
DRUG DEVELOPMENT RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Anna Stasilowicz-Krzemien, Natalia Rosiak, Andrzej Miklaszewski, Judyta Cielecka-Piontek
Summary: To improve the bioavailability of caffeic acid, delivery systems were developed using ball milling and freeze-drying techniques to prepare solid dispersions of caffeic acid and magnesium aluminometasilicate. The 1:1 mass ratio solid dispersion obtained from ball milling was the most effective, and its identity was confirmed using X-ray powder diffraction and Fourier-transform infrared spectroscopy techniques. Screening tests demonstrated the improved anti-neurodegenerative activity of caffeic acid with enhanced solubility, inhibiting acetylcholinesterase, butyrylcholinesterase, tyrosinase, and showing antioxidant potential. In silico studies identified the domains of caffeic acid that interacted with enzymes relevant to neuroprotective activity. Furthermore, improved permeability through gastrointestinal tract and blood-brain barrier mimicking membranes validated the in vivo screening tests for anti-neurodegenerative activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Marcela Safratova, Jana Kroustkova, Negar Maafi, Daniela Suchankova, Rudolf Vrabec, Jakub Chlebek, Jiri Kunes, Lubomir Opletal, Franz Bucar, Lucie Cahlikova
Summary: Clivia miniata, endemic to South Africa and Swaziland, is one of the top-10 traded medicinal plants in informal medicine markets in South Africa. It contains various Amaryllidaceae alkaloids, some of which have been identified as having neuroenzyme inhibition activity.
Article
Biochemistry & Molecular Biology
Sultan Darvesh, Scott Banfield, Maeve Dufour, Katrina L. Forrestall, Hillary Maillet, G. Andrew Reid, Dane Sands, Ian R. Pottie
Summary: The study aimed to determine whether the KR histochemical method could be used to evaluate probes at the site of pathology. The results suggest that the KR method may provide an efficient means to screen compounds as probes for imaging AD-associated ChEs.
JOURNAL OF ENZYME INHIBITION AND MEDICINAL CHEMISTRY
(2023)
Article
Cell Biology
Fernando Lopez Alvez, Natalia Pontes Bona, Nathalia Stark Pedra, Daniel Schuch da Silva, Wilson Joao Cunico, Francieli Moro Stefanello, Cinthia Melazzo de Andrade, Mayara Sandrielly Pereira Soares, Roselia Maria Spanevello
Summary: The study investigated the effect of DS27 on lipopolysaccharide-treated astrocytes. The findings showed that DS27 can reverse cellular damage induced by LPS and improve neuroinflammation.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2023)
Article
Endocrinology & Metabolism
Bernardo de Moraes Meine, Thais Scolari Franceschi, Natalia Pontes Bona, Luiza Spohr, Nathalia Stark Pedra, Roselia Maria Spanevello, Mayara Sandrielly Soares de Aguiar, Francieli Moro Stefanello
Summary: This study investigated the effects of chronic administration of methionine and its metabolite methionine sulfoxide on oxidative damage in the brain, liver, and kidney of young mice. The results showed that these substances increased reactive oxygen species and lipoperoxidation levels, while reducing antioxidant enzyme activities. Furthermore, they also affected the liver and kidney functions. These findings contribute to a better understanding of the pathophysiological mechanisms of hypermethioninemia.
METABOLIC BRAIN DISEASE
(2023)
Article
Neurosciences
Alessandra dos Santos, Fernanda Cardoso Teixeira, Daniel Schuch da Silva, Tayna Amaral Veleda, Julia Eisenhart de Mello, Karina Pereira Luduvico, Rejane Giacomelli Tavares, Francieli Moro Stefanello, Wilson Cunico, Roselia Maria Spanevello
Summary: Alzheimer's disease (AD) is characterized by memory decline and limited treatment options. Thiazolidin-4-one, a compound with multiple beneficial effects, was evaluated for its potential therapeutic effects in an AD model induced by streptozotocin (STZ). Treatment with DS12, a thiazolidin-4-one compound, improved memory and prevented neurochemical changes induced by STZ. DS12 also protected against oxidative damage in the brain without causing toxic effects.
BRAIN RESEARCH BULLETIN
(2023)
Review
Agronomy
Lara Baccarin Ianiski, Aline Fontanella Maciel, Caroline Quintana Braga, Carla Weiblen, Daniela Isabel Brayer Pereira, Luis Antonio Sangioni, Janio Morais Santurio, Luciana Potter, Sonia de Avila Botton
Summary: This study evaluated the use of nanotechnology in the treatment of Pythium insidiosum infections and found that nanotechnology can be a promising alternative for developing antimicrobial compounds with anti-P. insidiosum activity.
Article
Endocrinology & Metabolism
Julia Eisenhardt de Mello, Karina Pereira Luduvico, Alessandra dos Santos, Fernanda Cardoso Teixeira, Juliane de Souza Cardoso, Mayara Sandrielly Soares de Aguiar, Wilson Cunico, Marcia Vizzotto, Francieli Stefanello, Roselia Spanevello
Summary: The study evaluated the effects of blackberry extract on memory and neurochemical parameters in rats with scopolamine-induced amnesia. The results showed that blackberry extract can prevent memory impairment, reduce oxidative damage, and inhibit acetylcholinesterase activity caused by scopolamine. Therefore, blackberry extract has potential therapeutic applications for neurodegenerative diseases.
METABOLIC BRAIN DISEASE
(2023)
Article
Biochemistry & Molecular Biology
Luiza Spohr, Mayara Sandrielly Soares de Aguiar, Natalia Pontes Bona, Karina Pereira Luduvico, Amalia Goncalves Alves, William Borges Domingues, Eduardo Bierhals Blodorn, Cristiani Folharini Bortolatto, Cesar Augusto Bruning, Vinicius Farias Campos, Francieli Moro Stefanello, Roselia Maria Spanevello
Summary: This study found that blueberry extract improved depression-like behavior and attenuated neurochemical and molecular changes in the brains of mice challenged with LPS.
NEUROCHEMICAL RESEARCH
(2023)
Article
Chemistry, Analytical
Graciela M. Heidrich, Vinicius M. Neves, Naiara Stefanello, Vanessa V. Miron, Thauan F. Lopes, Sindy R. Krzyzaniak, Paola de A. Mello, Maria Rosa C. Schetinger, Dirce Pozebon, Valderi L. Dressler
Summary: Adult Wistar rats were exposed to lanthanum oxide nanoparticles (La(2)O(3)NPs) at different doses. Histological analysis revealed that higher doses of La2O3-NPs caused hepatic lesions in rats. However, the La2O3-NPs did not affect body weight, organ weight, and blood serum parameters. Increased reactive oxygen species (ROS) were found in the kidneys of rats treated with high dose of La2O3-NPs. Lanthanum was detected in organs but not in the blood.
BRAZILIAN JOURNAL OF ANALYTICAL CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Milagros Fanny Vera Castro, Charles Elias Assmann, Naiara Stefanello, Karine Paula Reichert, Tais Vidal Palma, Anielen Dutra da Silva, Vanessa Valeria Miron, Vitor Bastianello Mostardeiro, Vera Maria Melchiors Morsch, Maria Rosa Chitolina Schetinger
Summary: The study investigated the effects of caffeic acid (CA) on learning and memory tasks in diabetic rats. The researchers also assessed the impact of CA on the activities of specific enzymes and the density of certain receptors and inflammatory parameters in the cortex and hippocampus of diabetic rats. The results showed that CA improved learning and memory deficits, normalized enzyme activities, and modulated receptor density in the diabetic animals, suggesting its potential for treating cognitive deficits associated with the cholinergic and purinergic signaling pathways in diabetes. (c) 2023 Elsevier Inc. All rights reserved.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Neurosciences
Priscila Centeno Crespo, Leo Anderson Meira Martins, Clara Camacho Dos Reis, Liciane Fernandes Medeiros, Vanessa Leal Scarabelot, Giovana Duzzo Gamaro, Mayara Sandrielly Pereira Soares, Roselia Maria Spanevello, Francieli Moro Stefanello, Izabel Cristina Custodio De Souza
Summary: This study analyzed the effects of transcranial direct current stimulation (tDCS) on biochemical parameters in rats with neuropathic pain. The results showed that tDCS increased the total sulfhydryl content in the spinal cord of rats, positively modulating neuropathic pain.
NEUROSCIENCE LETTERS
(2023)
Review
Parasitology
Beni Jequicene Mussengue Chauque, Thaisla Cristiane Borella da Silva, Denise Leal dos Santos, Guilherme Brittes Benitez, Leosvilda Gomes Henriques Chauque, Antonio Domingues Benetti, Regis Adriel Zanette, Marilise Brittes Rott
Summary: The ubiquitous free-living amoebae (FLA) are microorganisms of significant medical, sanitary, and ecological importance. However, their characterization within solid matrices such as soil, dust, sediment, mud, sludge, and compost remain to be systematized. In this study, a systematic review with meta-analysis was conducted to explore the global distribution of FLA in solid matrices. The analysis of 104 out of 4,414 scientific articles revealed that FLA prevalence in solid matrices was 55.13% globally. Soil had the highest prevalence (72.40%), followed by sediment (57.91%), mud (52.90%), dust (48.60%), and sewage sludge (40.19%). Acanthamoeba spp. (52.23%) and Hartmanella/Vermamoeba spp. (36.06%) were the most prevalent FLA species.
Article
Veterinary Sciences
Diorges H. Setim, Carlos Bondan, Caroline C. Cortese, Jessica C. Peretti, Fernanda L. Facioli, Ricardo Zanella, Marcio M. Costa, Adriana C. da Motta
Summary: This study aimed to verify the presence of bovine paratuberculosis in commercial dairy herds in different mesoregions of RS, Brazil. Indirect ELISA tests and PCR were performed on milk and fecal samples, respectively. The results indicated that paratuberculosis is likely endemic in the northwest and northeast mesoregions.
PESQUISA VETERINARIA BRASILEIRA
(2023)
Article
Veterinary Sciences
J. H. Silva, L. V. Q. Contreras, C. F. Ponath, C. Bondan, M. C. Machado, F. Gonzalez
Summary: The study aimed to investigate the impact of pre and postpartum DCAD on serum calcium, phosphorus, and magnesium levels in peripartum Holstein cows. Blood samples were collected from 56 cows (18 primiparous and 38 multiparous) on different days relative to parturition to analyze mineral levels, while diet and urine samples were also collected. Prepartum DCAD showed a positive correlation with total and corrected calcium levels at day-7, and a negative correlation with magnesium levels at day-7. However, prepartum DCAD did not significantly affect phosphorus levels. Postpartum DCAD did not have any correlation with serum mineral levels. The study highlights the importance of considering parity and time period when evaluating mineral levels in Holstein cows during the transition period.
ARQUIVO BRASILEIRO DE MEDICINA VETERINARIA E ZOOTECNIA
(2023)
Article
Medicine, Research & Experimental
R. O. Leite, P. O. de Souza, C. B. Haas, F. da Silveira, K. R. Mohr, A. P. S. Bertoni, M. S. Soares, J. H. Azambuja, M. Dal Pra, L. L. P. da Cruz, N. E. Gelsleichter, K. Begnini, G. Hasko, M. R. Wink, R. M. Spanevello, E. Braganhol
Summary: Sepsis is a life-threatening organ dysfunction caused by a dysregulated inflammatory response to infection. Purinergic signaling, mediated by purines such as ATP and adenosine, plays a crucial role in the immune and inflammatory responses during sepsis. Sepsis disrupts the balance of ATP and adenosine levels, leading to dysregulation of immune responses. The measurement of serum levels and activities of purinergic pathway markers may serve as potential biomarkers for sepsis diagnosis and treatment.
CLINICAL AND EXPERIMENTAL MEDICINE
(2023)
Article
Biotechnology & Applied Microbiology
Bruna Pippi, Regis A. Zanette, Angelica R. Joaquim, Maria E. Krummenauer, Simone Merkel, Paula Reginatto, Marilene H. Vainstein, Saulo F. Andrade, Alexandre M. Fuentefria, Juliana S. M. Tondolo, Erico S. Loreto, Janio M. Santurio
Summary: This study evaluates the antimicrobial activity of three 8-hydroxyquinoline derivatives against Pythium insidiosum and determines their pharmacodynamic characteristics. Results show that Clioquinol, PH151, and PH153 are active against all isolates, with MIC values ranging from 0.25 to 2 μg/mL. They also exhibit a time- and dose-dependent antimicrobial effect, damaging the P. insidiosum cell wall. These findings reinforce the potential of 8-hydroxyquinoline derivatives as new drugs for treating pythiosis.
JOURNAL OF APPLIED MICROBIOLOGY
(2023)
Article
Plant Sciences
Natalia Pontes Bona, Mayara Sandrielly Soares de Aguiar, Luiza Spohr, Nathalia Stark Pedra, Francieli da Silva dos Santos, Juliane Torchelsen Saraiva, Fernando Lopez Alvez, Bernardo de Moraes Meine, Vania Recart, Ingrid Vicente Farias, Caroline Flach Ortmann, Roselia Maria Spanevello, Flavio Henrique Reginatto, Francieli Moro Stefanello
Summary: The extract and enriched flavonoid fraction from Cecropia pachystachya demonstrate neuroprotective and anti-inflammatory effects in a model of LPS-induced neuroinflammation, making them potential therapeutic targets for managing neurodegenerative diseases.
JOURNAL OF ETHNOPHARMACOLOGY
(2024)
Article
Veterinary Sciences
R. Mischke, K. Rumstedt, S. O. Hungerbuehler, K. Rohn, M. Schmicke
Summary: Contrast-enhanced ultrasound (CEUS) can provide an additional tool for the diagnosis of hypothyroidism in dogs and support its differentiation from non-thyroidal illness. Sedation has limited impact on CEUS results.
RESEARCH IN VETERINARY SCIENCE
(2024)
Review
Veterinary Sciences
Lucas Petitemberte de Souza, William Borges Domingues, Eduardo Bierhals Blodorn, Leandro da Silva Nunes, Hadassa Gabriela Ortiz, Eliza Rossi Komninou, Vinicius Farias Campos
Summary: This study performed a systematic review of the differential expression of sperm microRNAs in bulls related to fertility. A total of 182 unique differentially expressed miRNAs were identified, among which 49 miRNAs were found in common between at least two studies. The results suggest that miRNAs such as miR-10a, -10b, -103, -15b, -122, -125b, -126-5p, -151-5p, -193a-5p, -196a, -27a-5p, and -99b may serve as potential universal biomarkers for assessing the reproductive potential of males.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Wonhee Cha, Katinca Fungbrant, Giulio Grandi, Ylva Persson
Summary: This study investigated the prevalence and development of hair loss in outdoor cattle in Sweden. It found a relatively high hair loss rate, with the occurrence of hair loss increasing over time during winters, suggesting a contagious etiology. The study also identified preventative delousing before November, keeping animals clean, and maintaining small group sizes as effective measures to reduce the risk of hair loss, while older age and access to bedding materials increased the risk.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Sheeba Thomas, David Francis Lappin, David Bennett, Christopher Nile, Marcello Pasquale Riggio
Summary: This study found that Feline odontoclastic resorptive lesion (FORL) can be divided into two subgroups, with FORL-1 subgroup having a less diverse oral microbiome and higher levels of proinflammatory cytokines and chemokines, which may be associated with the severity and activity of the disease.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
S. L. Gayathri, M. Bhakat, T. K. Mohanty
Summary: This study used infrared thermography (IRT) to identify mastitis in Sahiwal cows. The results showed significant differences in the mean values of udder and teat surface temperature between healthy, subclinical mastitis (SCM), and clinical mastitis (CM) cases during different seasons. IRT is an efficient tool for the early identification of subclinical mastitis.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Zeynep Ozdemir Kutahya, Busra Aslan Akyol, Kubra Deliklitas, Cengiz Gokbulut
Summary: In this study, the pharmacokinetic behavior of Eprinomectin was studied in two different goat breeds. The results showed no significant differences in plasma concentration between the breeds, but the clearance from the body may vary. High concentration of Eprinomectin in feces is beneficial against gastrointestinal parasites but poses an ecotoxicological risk to the environment.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Mohammad Ashrafzadeh-Shiraz, Mousa Tavassoli, Bahram Dalir-Naghadeh, Alireza Sazmand
Summary: This study evaluated the efficacy of fenbendazole and ivermectin on strongyles and Parascaris sp. infecting adult riding horses in different regions of Iran. The results demonstrated varying degrees of resistance of these parasites to both anthelminthics. It emphasizes the urgency of educating horse owners about the importance of a test-and-treatment strategy and suggests evaluating the efficacy of different anthelminthics to choose the most effective treatment in each region.
RESEARCH IN VETERINARY SCIENCE
(2024)
Article
Veterinary Sciences
Moises Gonzalvez, Clara Munoz-Hernandez
Summary: The study evaluated the use of R software in research articles addressing the study of wildlife, showing an increasing trend in the number of publications over time and a wide geographical distribution globally.
RESEARCH IN VETERINARY SCIENCE
(2024)
