Simultaneous determination of serum mannose and glucose concentrations in dog serum using high performance liquid chromatography

Serum mannose and glucose concentrations in dogs before and after eating a meal were determined simultaneously with a recently established HPLC method combined with a UV and fluorescence detection system of p-aminobenzoic acid ethyl ester (ABEE)-derivatized monosaccharides. In this newly established HPLC method, detection limits were 0.09 mu mol/L for mannose and 0.04 mmol/L for glucose. Linearity of peak areas vs. amounts of mannose and glucose in the range of 0.27-320 mu mol/L and 0.13-64 mmol/L were observed, respectively. The value of the glucose content measured by the HPLC method was in good agreement with that of the commonly used enzymatic method (control). Serum glucose concentrations in dogs 90 min after the meal were almost the same as those before the meal, whereas serum mannose concentrations decreased significantly after the meal. This HPLC method may be useful for determination of monosaccharides in animal blood. (C) 2007 Elsevier Ltd. All rights reserved.