期刊
RESEARCH IN MICROBIOLOGY
卷 164, 期 4, 页码 288-292出版社
ELSEVIER
DOI: 10.1016/j.resmic.2013.01.007
关键词
Long-term preservation; Ammonia-oxidizing bacteria; Cryoprotective agent
类别
资金
- Geconcerteerde Onderzoeksactie (GOA) of Ghent University [BOF09/GOA/005]
Long-term storage of the fastidious ammonia-oxidizing bacteria has proven difficult, which limits their public availability and results in a loss of cultured biodiversity. To enable their accessibility to the scientific community, an effective protocol for cryopreservation of ammonia-oxidizing cultures at -80 degrees C and in liquid nitrogen was developed. Long-term storage could be achieved using 5% DMSO as cryoprotectant, preferably in a cryoprotective preservation medium containing tenfold-diluted trypticase soy broth and 1% trehalose. As such, successful activity and growth recovery was observed for a diverse set of ammonia-oxidizing cultures. (c) 2013 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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