期刊
RESEARCH IN MICROBIOLOGY
卷 163, 期 2, 页码 83-91出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2011.10.009
关键词
Polyamines; Antibiotic resistance; Reactive oxygen species; DNA damage
类别
资金
- Presidium RAS Molecular and cellular biology [0120096390]
- Russian Foundation for Basic Research [09-04-99006, 11-04-96001]
Bactericidal antibiotics (fluoroquinolones, aminoglycosides and cephalosporins) at their sublethal concentrations were able to produce hydroxyl radicals, hydrogen peroxide and superoxide anions (ROS) in Escherichia coli cells, which resulted in damage to proteins and DNA. The cells responded to oxidative stress by a 2-3-fold increase in cell polyamines (putrescine, spermidine) produced as a consequence of upregulation of ornithine decarboxylase (ODC). Relief of oxidative stress by cessation of culture aeration or addition of antioxidants substantially diminished or even completely abolished polyamine accumulation observed in response to antibiotics. Alternatively, inhibition of polyamine synthesis resulted in enhancement of oxidative stress in antibiotic-processed cells. When added to antibiotic-inhibited culture, polyamines reduced intracellular ROS production and thereby prevented damage to proteins and DNA. These effects eventually resulted in a substantial increase in cell viability, growth recovery and antibiotic resistance that were more strongly expressed in polyamine-deficient mutants. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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