期刊
RESEARCH IN MICROBIOLOGY
卷 162, 期 5, 页码 514-519出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2011.03.009
关键词
Bifidobacteria; Bile-adapted strain; Human gastric juice; Human duodenal juice; HT29-MTX cell line; Gastrointestinal transit simulation (GITTS)
类别
资金
- European Union
- Spanish Plan Nacional de I+D+I [AGL2004-6088, AGL2007-62736, AGL2009-09445]
According to the FAO/WHO, in vitro criteria for selection of probiotics for food application consist of testing survival when confronted with gastrointestinal tract (GIT) challenge and the ability to colonize the colon. We used a model that simulated GIT transit using sequential immersion in gastric and duodenal juices of human origin to evaluate survival of bile-adapted Bifidobacterium strains. Bifidobacterium animalis tolerated gastric juice, whereas Bifidobacterium ion gum showed poor survival under these conditions. In contrast, B. animalis strains were more sensitive to duodenal juice than B. longum. The percentage of survival after GIT transit simulation (GITTS), determined both by plate counts and fluorescent probes, was significantly higher for bile-adapted strains than for corresponding parental ones. This suggests that use of bile-adapted strains is a suitable approach for increasing survival of bifidobacteria under the harsh conditions of the upper GIT. However, the bile resistance phenotype was not related to improvement of adhesion capacity, after GITTS, of the intestinal cell line HT29-MTX which constitutively produces mucus. This work shows that sequential GITTS with human juices modified the in vitro adhesion properties of the strains challenged with colonocyte-like cells. (C) 2011 Institut Pasteur. Published by Elsevier Masson SAS. All rights reserved.
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