Article
Biochemistry & Molecular Biology
Noah Hitchcock, David J. Kelly, Andrew Hitchcock, Aidan J. Taylor
Summary: This study found that the cysM gene of Campylobacter jejuni encodes an O-acetyl-L-serine sulfhydrylase with high selectivity for sulfide, enabling it to efficiently utilize sulfide as a sulfur source in locations such as the intestine. However, high concentrations of sulfide inhibit the growth of C. jejuni, and further research is needed to identify the cellular targets responsible for sulfide toxicity.
Article
Chemistry, Multidisciplinary
Roman Rohac, Lydie Martin, Liang Liu, Debashis Basu, Lizhi Tao, R. David Britt, Thomas B. Rauchfuss, Yvain Nicolet
Summary: FeFe-hydrogenases use a unique H cluster to convert H-2 into protons and low-potential electrons. The [2Fe](H) center is where the reaction occurs and is built stepwise by maturating enzymes, with HydE performing complex modifications of complex-B to produce a precursor to the [2Fe](H) center.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Biotechnology & Applied Microbiology
Zerlina G. Wuisan, I. Dewa M. Kresna, Nils Boehringer, Kim Lewis, Till F. Schaeberle
Summary: Darobactin A (DAR) is a promising antibiotic targeting Gram-negative bacteria, initially identified in bacteria from several genera. It targets the outer membrane protein BamA and has shown convincing in vivo activities in mouse infection models. By establishing a heterologous production and engineering platform, DAR production yield can be improved and structure modification approaches can be facilitated. Only two genes are necessary for the heterologous production of this RiPP antibiotic.
METABOLIC ENGINEERING
(2021)
Article
Astronomy & Astrophysics
M. Laine, S. Procacci
Summary: The friction experienced by a field in a medium depends on its evolution rate compared to the medium's time scales as the medium gradually heats up. A framework is proposed to incorporate contributions from all medium time scales, with warm axion inflation used as an example. By inputting the retarded pseudoscalar correlator of a thermal Yang-Mills plasma, the system's evolution from almost vanishing temperature to entering the weak or strong regime of warm inflation is demonstrated, scrutinizing previous approximate treatments.
JOURNAL OF COSMOLOGY AND ASTROPARTICLE PHYSICS
(2021)
Article
Biochemistry & Molecular Biology
Dao Feng Xiang, James B. Thoden, Manas K. Ghosh, Hazel M. Holden, Frank M. Raushel
Summary: Campylobacter jejuni, a common cause of food poisoning, has different serotypes with distinct sugar sequences and modifications in its capsular polysaccharide. This study reports the characterization and structure of an enzyme involved in the synthesis of the capsular polysaccharide in C. jejuni 81-176.
Article
Gastroenterology & Hepatology
Lea Henneke, Kristina Schlicht, Nadia A. Andreani, Tim Hollstein, Tobias Demetrowitsch, Carina Knappe, Katharina Hartmann, Julia Jensen-Kroll, Nathalie Rohmann, Daniela Pohlschneider, Corinna Geisler, Dominik M. Schulte, Ute Settgast, Kathrin Tuerk, Johannes Zimmermann, Christoph Kaleta, John F. Baines, Jane Shearer, Shrushti Shah, Grace Shen-Tu, Karin Schwarz, Andre Franke, Stefan Schreiber, Matthias Laudes
Summary: Recent research has found that Parasutterella plays an important role in the development of type 2 diabetes and obesity. This study analyzed a European cohort and validated the results in a Canadian cohort. The findings showed that Parasutterella was positively associated with BMI and type 2 diabetes, and its abundance was related to the intake of carbohydrates. Furthermore, the study found a reduction in L-cysteine and activation of the fatty acid biosynthesis pathway, suggesting potential mechanisms for the development of type 2 diabetes and obesity.
Article
Biology
Stephanie Peters, Ben Pascoe, Zuowei Wu, Sion C. Bayliss, Ximin Zeng, Adam Edwinson, Sakteesh Veerabadhran-Gurunathan, Selina Jawahir, Jessica K. Calland, Evangelos Mourkas, Robin Patel, Terra Wiens, Marijke Decuir, David Boxrud, Kirk Smith, Craig T. Parker, Gianrico Farrugia, Qijing Zhang, Samuel K. Sheppard, Madhusudan Grover
Summary: Researchers used whole-genome sequencing and phenotypic analysis to identify potential genetic factors linked to irritable bowel syndrome caused by Campylobacter jejuni infection. They found that specific genetic variations in bacterial stress response, adhesion protein, and core biosynthetic pathway genes may contribute to the development of post-infection irritable bowel syndrome (PI-IBS). The study demonstrated that certain Campylobacter genotypes exhibit increased virulence in vitro, shedding light on the complex host-pathogen interactions underlying this condition.
COMMUNICATIONS BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Harald Nothaft, Xiaoming Bian, Asif Shajahan, William G. Miller, David T. Bolick, Richard L. Guerrant, Parastoo Azadi, Kenneth K. S. Ng, Christine M. Szymanski
Summary: The study identified C. jejuni isolates expressing a hexasaccharide lacking the glucose branch, caused by a missing aspartate in the DXDD motif of the glucosyltransferase PglI. Mouse vaccination with E. coli expressing the full-length heptasaccharide demonstrated reduction in C. jejuni colonization and weight loss. The study suggests the importance of considering glucose inclusion in N-glycan-based vaccines for preventing campylobacteriosis.
ACS CHEMICAL BIOLOGY
(2021)
Article
Chemistry, Multidisciplinary
H. Estheban Osorio Franco, Brennan T. Chaloux, Matthew C. T. Hartman
Summary: Peptide macrocycles (PMCs) are popular for developing inhibitors of protein-protein interactions (PPIs). Large libraries of PMCs can be accessed using display technologies. The non-canonical amino acid pCAF enables spontaneous, co-translational cyclization under physiological conditions, creating stable macrocycles of various ring sizes.
CHEMICAL COMMUNICATIONS
(2022)
Article
Microbiology
Brittni R. Kelley, Sean M. Callahan, Jeremiah G. Johnson
Summary: The transcriptional regulator HeuR in Campylobacter jejuni plays a role in positively regulating heme utilization and negatively regulating methionine biosynthesis. Mutations in HeuR result in overexpression of metC, impacting bacterial growth and invasion ability under nutrient-replete conditions and iron limitation. This study highlights the importance of regulating methionine biosynthesis for adherence and invasion in the gastrointestinal tract.
JOURNAL OF BACTERIOLOGY
(2021)
Article
Multidisciplinary Sciences
Jamieson B. McDonald, Nichollas E. Scott, Greg J. Underwood, Daniel M. Andrews, Thi Thu Hao Van, Robert J. Moore
Summary: Campylobacter hepaticus is a significant pathogen causing Spotty Liver Disease in layer chickens, leading to increased mortality and reduced egg production. This study confirms the functionality of the N-glycosylation system in C. hepaticus through glycoproteomic analysis, which showed the modification of over 35 proteins with an N-linked heptasaccharide glycan. C. hepaticus shares conserved glycoproteins with Campylobacter jejuni involved in host colonization and possesses unique glycoproteins that may contribute to its survival in challenging host environments.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Organic
Saba Homayonia, Pengfei Zhang, Ping Zhang, Chang-Chun Ling
Summary: Campylobacter jejuni is a bacterial pathogen that causes millions of food-borne gastroenteritis cases annually and is associated with serious autoimmune sequelae. The unique polysaccharide structure of C. jejuni HS:4 has potential for eliciting protective antibodies. However, its chemical synthesis is challenging. This study reports an efficient synthesis of the key repeating disaccharide and its analog using a one-step conversion method.
ORGANIC & BIOMOLECULAR CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
De-Wei Gao, Cooper S. Jamieson, Gaoqian Wang, Yan Yan, Jiahai Zhou, K. N. Houk, Yi Tang
Summary: Medium-ring lactones are synthetically challenging due to unfavorable energetics in cyclization. The enzyme DcsB from the decarestrictine C1 biosynthetic pathway has been discovered to efficiently catalyze medium-ring lactonizations, showing broad substrate promiscuity. Insights into the molecular basis of catalysis have been gained through X-ray crystal structure and computational analyses.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Agricultural Engineering
Jihee Yoon, Jiyun Bae, Seulgi Kang, Byung-Kwan Cho, Min-Kyu Oh
Summary: This study successfully improved the production of bioplastic poly-3-hydroxybutyrate (PHB) using acetate as the sole carbon source through metabolic engineering and the supply of energy cofactors. The yields of biomass and PHB from acetate significantly improved, and the growth rate and PHB content of the bacteria increased. The sustainability of PHB production was demonstrated by using acetate derived from carbon dioxide and syngas.
BIORESOURCE TECHNOLOGY
(2022)
Article
Chemistry, Physical
Marialaura Marchetti, Francesco Saverio De Angelis, Giannamaria Annunziato, Gabriele Costantino, Marco Pieroni, Luca Ronda, Andrea Mozzarelli, Barbara Campanini, Salvatore Cannistraro, Anna Rita Bizzarri, Stefano Bettati
Summary: Cysteine is crucial for microbial survival and pathogenicity, with inhibitors targeting enzymes involved in cysteine biosynthesis showing potential as new antibacterial agents. Studying the interaction between enzymes in cysteine synthesis pathways could lead to the development of broad-spectrum antimicrobial compounds.
Review
Microbiology
Virginie Dufour, Martin Stahl, Christine Baysse
Article
Biochemistry & Molecular Biology
Bethany Nancolas, Ian D. Bull, Richard Stenner, Virginie Dufour, Paul Curnow
Article
Biotechnology & Applied Microbiology
Virginie Dufour, Martin Stahl, Eric Rosenfeld, Alain Stintzi, Christine Baysse
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2013)
Article
Microbiology
Catherine Fontenelle, Carlos Blanco, Morgane Arrieta, Virginie Dufour, Annie Trautwetter
Article
Microbiology
Virginie Dufour, Jennifer Li, Annika Flint, Eric Rosenfeld, Katell Rivoal, Sylvie Georgeault, Bachar Alazzam, Gwennola Ermel, Alain Stintzi, Martine Bonnaure-Mallet, Christine Baysse
Article
Immunology
Virginie Dufour, Bachar Alazzam, Gwennola Ermel, Marion Thepaut, Albert Rossero, Odile Tresse, Christine Baysse
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2012)