Article
Immunology
Angel Rodriguez Villodres, Lydia Galvez Benitez, Manuel J. Arroyo, Gema Mendez, Luis Mancera, Andrea Vila Dominguez, Jose Antonio Lepe Jimenez, Younes Smani
Summary: The study demonstrates that the MALDIpiptaz test can efficiently and rapidly discriminate between P/T-resistant, ESRI developers, and P/T-susceptible isolates in E. coli. Its combination of excellent performance and cost-effectiveness make it a useful tool for the rapid determination of P/T resistance in clinically relevant E. coli isolates.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Microbiology
Jose Manuel Ortiz de la Rosa, Angel Rodriguez-Villodres, Maria Adelina Gimeno Gascon, Guillermo Martin-Gutierrez, Jose Miguel Cisneros, Jose Antonio Lepe
Summary: As resistance to TZP in K. pneumoniae and E. coli increases, rapid determination of susceptibility to piperacillin-tazobactam (TZP) is crucial for the treatment of infected patients. In this study, the RapidTZP test was developed to detect TZP resistance in a maximum of 3h, with a sensitivity and specificity of 98.24% and 100%, respectively.
MICROBIOLOGY SPECTRUM
(2023)
Article
Infectious Diseases
Lydia Galvez-Benitez, Jose Manuel Ortiz de la Rosa, Angel Rodriguez-Villodres, Carlos S. Casimiro-Soriguer, Irene Molina-Panadero, Rocio Alvarez-Marin, Remy A. Bonnin, Thierry Naas, Jeronimo Pachon, Jose Miguel Cisneros, Jose Antonio Lepe, Younes Smani
Summary: This study aimed to identify the mechanisms underlying Piperacillin-tazobactam resistance (P/T-R) in Escherichia coli. The results showed that P/T-R isolates had a higher copy number of the bla(TEM) gene, generated by three different genetic events. In addition, reduced expression of OmpC was observed in two P/T-R strains. This study provides important insights into the acquisition of P/T-R in E. coli, which is crucial for effective treatment and prevention of resistant microorganisms.
JOURNAL OF INFECTION
(2023)
Article
Microbiology
Angel Rodriguez-Villodres, Alicia Gutierrez Linares, Lydia Galvez-Benitez, Jeronimo Pachon, Jose Antonio Lepe, Younes Smani
Summary: A method for detecting TZP resistance and ESRI in E. coli was designed and validated, showing great efficacy in detecting TZP-intermediate or -resistant isolates and TZP-susceptible isolates with the capacity for ESRI development. The method could potentially reduce health costs, ineffective treatments, and inappropriate antibiotic use.
MICROBIOLOGY SPECTRUM
(2021)
Article
Genetics & Heredity
Thomas Edwards, Eva Heinz, Jon van Aartsen, Alex Howard, Paul Roberts, Caroline Corless, Alice J. Fraser, Christopher T. Williams, Issra Bulgasim, Luis E. Cuevas, Christopher M. Parry, Adam P. Roberts, Emily R. Adams, Jenifer Mason, Alasdair T. M. Hubbard
Summary: This study investigates the genetic diversity and resistance mechanisms of Escherichia coli strains with phenotypic resistance to piperacillin/tazobactam but susceptibility to third-generation cephalosporins. The findings reveal a wide range of sequence types and plasmids, as well as diverse resistance mechanisms associated with this phenotype.
MICROBIAL GENOMICS
(2022)
Article
Infectious Diseases
Kamrul Islam, Fekade B. Sime, Michelle J. Bauer, Brian M. Forde, Steven C. Wallis, Patrick Harris, Saiyuri Naicker, Tahmina Shirin, Zakir H. Habib, Meerjady S. Flora, Jason A. Roberts
Summary: The study demonstrated that piperacillin/tazobactam (TZP) exhibited good bactericidal activity against ESBL-producing and non-ESBL-producing Escherichia coli, and prevented the emergence of resistance effectively.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Infectious Diseases
Kamrul Islam, Fekade B. Sime, Michelle J. Bauer, Brian M. Forde, Steven C. Wallis, Patrick Harris, Saiyuri Naicker, Tahmina Shirin, Zakir H. Habib, Meerjady S. Flora, Jason A. Roberts
Summary: The standard dosing regimens of piperacillin/tazobactam (TZP) effectively suppressed both ESBL-producing and non-ESBL-producing Escherichia coli, preventing the emergence of resistance.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Microbiology
Kamrul Islam, Fekade B. Sime, Steven C. Wallis, Michelle J. Bauer, Saiyuri Naicker, Hayoung Won, Hosam M. Zowawi, Md Abu Choudhury, Tahmina Shirin, Zakir H. Habib, Patrick N. A. Harris, Meerjady S. Flora, Jason A. Roberts
Summary: For ESBL-producing, piperacillin-tazobactam resistant E. coli, both piperacillin-tazobactam/amikacin combination therapy and meropenem monotherapy showed rapid bacterial killing without the emergence of resistant subpopulations.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2022)
Article
Microbiology
Kosuke Hoashi, Brian Hayama, Masahiro Suzuki, Aki Sakurai, Kazumi Takehana, Taisuke Enokida, Koichi Takeda, Daisuke Ohkushi, Yohei Doi, Sohei Harada
Summary: Piperacillin-tazobactam may be an effective option for treating ESBL-producing Escherichia coli bacteremia in areas with low frequency of OXA-1 co-production.
MICROBIOLOGY SPECTRUM
(2022)
Article
Biology
Josiah C. Kratz, Shiladitya Banerjee
Summary: A quantitative theory is developed to explain the relationship between bacterial growth and division rates and proteome allocation in time-varying nutrient environments. The theory shows that in such environments, cell size and growth are regulated by trade-offs between biomass accumulation and division. The study also finds that bacteria exhibit a transient memory of previous metabolic states, allowing for faster adaptation to previously encountered environments.
COMMUNICATIONS BIOLOGY
(2023)
Article
Microbiology
Alexander Lawandi, Gleice C. Leite, Matthew P. Cheng, Brigitte Lefebvre, Jean Longtin, Todd C. Lee
Summary: The combination of piperacillin-tazobactam and meropenem showed synergistic effects against KPC and OXA-48 carbapenemase-producing strains, but not against NDM-1-producing isolates.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Cell Biology
Timothy Kudinha, Fanrong Kong
Summary: The prevalence of ST131, especially among ciprofloxacin resistant isolates, increased from fecal to clinical samples in women, men, and children. ST131 isolates showed higher virulence potential compared to non-ST131 isolates, particularly in urinary samples. There was a strong association between ST131 prevalence and fluoroquinolone resistance, with FQ resistant ST131 isolates harboring more virulence genes.
JOURNAL OF BIOMEDICAL SCIENCE
(2022)
Article
Microbiology
Carola Venturini, Bethany Bowring, Alicia Fajardo-Lubian, Carol Devine, Jonathan Iredell
Summary: The study found that different antibiotics have a significant impact on the gut microbiota of mice, with third-generation cephalosporins causing more severe dysbiosis. Even after the antibiotics are stopped, the gut of mice remains highly susceptible to colonization by antibiotic-resistant enterobacterial pathogens. Co-habitation of untreated mice with treated mice can mitigate the dysbiosis caused by treatment.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Immunology
John Cao, Yanina Dubrovskaya, Justin Siegfried, Arnold Decano, Dana Mazo, Sarah Hochman, Ioannis M. Zacharioudakis, Jonathan So, Sadie Solomon, John Papadopoulos, Kassandra Marsh
Summary: This retrospective study compared the treatment strategies for TZP-NS/CRO-S E coli or K pneumoniae infections in noncritically ill adults. The results showed no significant difference in clinical outcomes between patients who received carbapenem and those who received carbapenem-sparing agents.
OPEN FORUM INFECTIOUS DISEASES
(2023)
Article
Microbiology
Carmila Manuel, Richard Maynard, Romney M. Humphries
Summary: Globally, piperacillin-tazobactam resistance among Escherichia coli and Klebsiella pneumoniae is mainly driven by OXA-1 beta-lactamases. The variability of the reference broth microdilution method (BMD) and ETEST in differentiating isolates with and without bla(OXA-1) gene was evaluated. ETEST showed lower accuracy and higher false resistance compared to BMD.
JOURNAL OF CLINICAL MICROBIOLOGY
(2022)
Article
Microbiology
Barbara Ellen Santos Carvalhais, Cristiana de Souza e Silva, Kenia Valeria dos Santos
Summary: The study evaluated the activity of different antimicrobials against Stenotrophomonas maltophilia biofilms at different stages, with surprising activity of gentamicin against the biofilms. Chloramphenicol and trimethoprim-sulfamethoxazole were indicated as good options for young biofilms, while trimethoprim-sulfamethoxazole and ceftazidime showed limited antibiofilm activity in mature biofilms.
FUTURE MICROBIOLOGY
(2021)
Review
Chemistry, Medicinal
Samuel Cavalcante do Amaral, Patrick Romano Monteiro, Joaquim da Silva Pinto Neto, Gustavo Marques Serra, Evonnildo Costa Goncalves, Luciana Pereira Xavier, Agenor Valadares Santos
Summary: Microviridins, a class of ribosomal peptides derived from cyanobacteria, are potent protease inhibitors with potential applications in drug development and mosquito control. They also play a key ecological role in defending cyanobacteria against microcrustaceans. This review aims to systematically identify the key characteristics of microviridins, including their chemical structure, biosynthesis, as well as their biotechnological and ecological significance.
Article
Polymer Science
Murilo Moraes Mourao, Luciana Pereira Xavier, Ralph Urbatzka, Lucas Barbosa Figueiroa, Carlos Emmerson Ferreira da Costa, Carmen Gilda Barroso Tavares Dias, Maria Paula Cruz Schneider, Vitor Vasconcelos, Agenor Valadares Santos
Summary: The study investigated the possibility of using lignocellulosic agro-industrial waste as carbon sources for PHB biosynthesis by Amazonian microalga Stigeoclonium sp. B23. Results showed that PHB produced by the microalga from cassava peel hydrolysate could be a promising biomaterial, but also exhibited toxicity on zebrafish embryos.
Article
Food Science & Technology
Lucas Guzella Carvalho, Mariana Massi Afonso Alvim, Rodrigo Luiz Fabri, Ana Carolina Morais Apolonio
Summary: The study revealed that Staphylococcus aureus can form biofilm and detach in Minas Frescal cheese packaging at 5 degrees Celsius, suggesting improvements in packaging hygiene are strongly recommended.
INTERNATIONAL JOURNAL OF DAIRY TECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Fabio Jose Bonfim Cardoso, Luciana Pereira Xavier, Agenor Valadares Santos, Humberto D'Muniz Pereira, Lourivaldo da Silva Santos, Fabio Alberto de Molfetta
Summary: Neolignans show potential inhibition of the enzyme activity of Schistosoma mansoni, with molecular docking and dynamics studies indicating stable inhibition. Biological assays suggest neolignans as promising candidates for future chemotherapy research on schistosomiasis.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Infectious Diseases
Rodrigo L. Fabri, Jhamine C. O. Freitas, Ari S. O. Lemos, Lara M. Campos, Irley O. M. Diniz, Nicolas C. C. Pinto, Thiago P. Silva, Cinthia Palazzi, Paula Marchesini, Caio Monteiro, Alan F. Barbosa, Mario G. Carvalho, Luciana M. Chedier, Marcelo G. F. Araujo, Ana Carolina M. Apolonio, Vinicius N. Rocha, Rossana C. N. Melo, Priscila F. Pinto
Summary: This study evaluated the antifungal activity of spilanthol against Candida albicans ATCC (R) 10231 (TM), a multidrug-resistant fungal strain. The findings showed that spilanthol(AcO) has the potential to be considered for the therapeutical treatment of vulvovaginal candidiasis caused by C. albicans.
Article
Multidisciplinary Sciences
Ari S. O. Lemos, Lara M. Campos, Thalita F. Souza, Juliana T. Granato, Erick E. Oliveira, Danielle M. O. Aragao, Ana Carolina M. Apolonio, Ana Paula Ferreira, Rodrigo L. Fabri
Summary: This study determined the chemical profile of the ethanolic extract of C. coriaceum leaves using UFLC-QTOF-MS and evaluated its in vitro biological potential. The results showed that CCE exhibited antifungal, antioxidant, and anti-inflammatory activities.
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
(2022)
Article
Green & Sustainable Science & Technology
Lucas Rafael Santana Pinheiro, Diana Gomes Gradissimo, Luciana Pereira Xavier, Agenor Valadares Santos
Summary: The use of dyes, especially azo dyes, has been increasing due to population and industrial growth, resulting in significant environmental impact. Bacteria offer a versatile solution for the bioremediation of environments contaminated with azo dyes, with the potential to combine biodegradation and economic product production. Bacteria can adapt to various chemical and physical parameters, showing good performance under industry and environmental conditions. This review highlights promising research on the use of bacteria in the detoxification and degradation of azo dyes, discussing mechanisms and legislation, emphasizing the importance of bacterial bioremediation in the pursuit of sustainable production.
Article
Public, Environmental & Occupational Health
Julia Pereira Carvalho Almeida, Robert Willer Farinazzo Vitral, Aneliese Holetz de Toledo Lourenco, Georgia Botafogo Pinheiro das Flores e Cruz D'almas Coxipones, Ana Barbara Polo, Ana Carolina Morais Apolonio
Summary: The study found that moisture may assist bacteria in penetrating sterilized SMS packaging, thereby breaking the aseptic chain. Therefore, handling and storing sterile packaging must be done carefully to avoid potential risks.
ACTA SCIENTIARUM-HEALTH SCIENCES
(2022)
Article
Infectious Diseases
Renata da Costa Barros Silva, Jessica Andrade, Vanessa Cordeiro Dias, Jessica Carla Sequeto, Nayara Felga Santos, Vania Lucia da Silva, Claudio Galuppo Diniz
Summary: This study examined the antibiotic susceptibility patterns and physiological aspects of enterobacteria isolated from meat and fish samples in a Brazilian urban Centre. The majority of isolated bacteria were resistant to at least one antimicrobial, with some showing resistance to multiple drugs. The multidrug-resistant isolates exhibited higher tolerance to toxic metals and increased activity of efflux pumps.
Review
Chemistry, Medicinal
Samuel Cavalcante do Amaral, Luciana Pereira Xavier, Vitor Vasconcelos, Agenor Valadares Santos
Summary: Cyanobacteria are a valuable source of secondary metabolites with diverse chemical and biological properties, including antifungal activity. This review examines the key features of these antifungal compounds, their sources, major targets, and the environmental factors involved in their production. A comprehensive analysis of 642 documents dating from 1980 to 2022, including patents, original research, review articles, and theses, was conducted for this study.
Article
Microbiology
Gustavo Marques Serra, Andrei Santos Siqueira, Fabio Alberto de Molfetta, Agenor Valadares Santos, Luciana Pereira Xavier
Summary: In this study, the beta-glucosidase from Microcystis aeruginosa CACIAM 03 (MaBgl3) was evaluated for its potential in cellulosic biomass bioconversion. The results showed that MaBgl3 is a stable enzyme with favorable binding free energy, making it suitable for cellulose hydrolysis.
Article
Microbiology
Ana Polo, Ari Lemos, Camila Martins da Mata, Veronica Oliveira, Ana Pontes, Daniel Pontes, Guilherme Tavares, Rodrigo Fabri, Ana Carolina Apolonio
Summary: This study evaluated the effect of a new Fe-cyclam complex on pathogenic bacterial species, including multidrug-resistant clinical specimens. The complex showed broad bacterial activity, mainly against Cutibacterium acnes, S. aureus, Pseudomonas aeruginosa, and Acinetobacter baumannii. The tested compound proved to be a promising agent against resistant bacterial infections.
FUTURE MICROBIOLOGY
(2023)
Review
Polymer Science
Thiago Medeiros Zacaron, Mariana Leite Simoes e Silva, Mirsiane Pascoal Costa, Dominique Mesquita e Silva, Allana Carvalho Silva, Ana Carolina Morais Apolonio, Rodrigo Luiz Fabri, Frederico Pittella, Helvecio Vinicius Antunes Rocha, Guilherme Diniz Tavares
Summary: The evolution of respiratory diseases, including emerging respiratory diseases caused by coronavirus family, presents a major public health challenge. Nanomedicine, specifically pulmonary drug delivery using chitosan nanoparticles, has shown potential in optimizing treatment efficacy by increasing drug bioavailability and reducing side effects. The unique biological properties of chitosan nanoparticles, such as anti-inflammatory and antimicrobial capabilities, make them advantageous as nanocarriers for drug delivery to the lungs.
Article
Dentistry, Oral Surgery & Medicine
Raphaella Barcellos Fernandes, Ana Barbara Polo, Vinicius Novaes Rocha, Robert Willer Farinazzo Vitral, Ana Carolina Morais Apolonio, Marcio Jose da Silva Campos
Summary: This study compared the surface properties of self-ligating metallic, ceramic esthetic, and conventional metallic brackets. It also evaluated the adhesion of Streptococcus mutans biofilms to their surface. The results showed that despite higher surface roughness, ceramic brackets did not significantly influence the adhesion of S. mutans biofilms.
SAUDI DENTAL JOURNAL
(2022)