Article
Plant Sciences
Chananwat Kortheerakul, Hakuto Kageyama, Rungaroon Waditee-Sirisattha
Summary: The study identified and characterized the GST gene family in the halotolerant cyanobacterium Halothece sp. PCC7418. One of the GSTs, H3557, showed significant upregulation under salt stress and exhibited GST activity towards CDNB and GSH with a broad range of enzymatic activity. This salt-adaptive enzyme remained catalytically active in the presence of NaCl and conferred tolerance to H2O2-induced oxidative stress and salt stress in heterologous expression systems.
PLANT CELL AND ENVIRONMENT
(2021)
Article
Biochemistry & Molecular Biology
Bethilda Anne Selvaraj, Abdul Razak Mariatulqabtiah, Kok Lian Ho, Chyan Leong Ng, Chean Yeah Yong, Wen Siang Tan
Summary: This study aims to enhance the stability of MrNV CP by modulating protease degradation activity. The addition of a specific protease inhibitor E-64 significantly increased the yield of MrNV CP, providing an economical method to improve scalability for downstream applications such as drug delivery and vaccine development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Nobutoshi Yamaguchi, Satoshi Matsubara, Kaori Yoshimizu, Motohide Seki, Kouta Hamada, Mari Kamitani, Yuko Kurita, Yasuyuki Nomura, Kota Nagashima, Soichi Inagaki, Takamasa Suzuki, Eng-Seng Gan, Taiko To, Tetsuji Kakutani, Atsushi J. Nagano, Akiko Satake, Toshiro Ito
Summary: Acclimation to high temperature increases plants' tolerance of subsequent lethal high temperatures. The study reveals that JUMONJI H3K27me3 demethylases are essential for heat acclimation in Arabidopsis. This epigenetic memory mechanism involving histone demethylases could have significant implications for environmental adaptation of field plants.
NATURE COMMUNICATIONS
(2021)
Review
Plant Sciences
Sellwane Jeanette Moloi, Rudo Ngara
Summary: Upon drought exposure, plants undergo changes in gene, protein, and metabolite expression through complex signal transduction events. Proteomics studies have identified numerous drought-responsive proteins involved in drought adaptation, including protein degradation processes that activate enzymes and signalling peptides, recycle nitrogen sources, and maintain protein turnover and homeostasis. This review focuses on the differential expression and functional activities of plant protease and protease inhibitor proteins under drought stress, particularly comparative studies of genotypes with contrasting drought phenotypes. The review also explores the role of transgenic plants overexpressing or repressing proteases or their inhibitors in drought response. Protease degradation plays a vital role in plant survival under water deficits, regardless of genotype's drought resilience. However, drought-sensitive genotypes exhibit higher proteolytic activities, while drought-tolerant genotypes protect proteins by expressing more protease inhibitors. Additionally, proteases and protease inhibitors are implicated in various physiological functions under drought stress, including stomatal regulation, maintenance of water content, phytohormonal signaling, and induction of stress genes. More validation studies are needed to further understand the functions of proteases and their inhibitors under water limitation and their contributions to drought adaptation.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Ecology
Le Liu, Xiaofeng Chen, Jianing Ye, Xiaoyi Ma, Yu Han, Yajie He, Kai Tang
Summary: Sulfoquinovose (SQ) is an abundant organosulfur compound in the biosphere, and marine bacteria, particularly Dinoroseobacter shibae DFL 12 and Roseobacter denitrificans OCh 114, have been found to be capable of metabolizing SQ. The bacteria possess specific proteins that enable them to break down SQ for energy, while down-regulating proteins involved in sulfite production via assimilatory sulfate reduction. Additionally, analysis of marine samples further supports the metabolism of SQ by these bacteria, highlighting a previously unidentified link in the marine sulfur cycle.
Article
Microbiology
Laura Alonso-Saez, Antonio S. Palacio, Ana M. Cabello, Semidan Robaina-Estevez, Jose M. Gonzalez, Laurence Garczarek, Angel Lopez-Urrutia
Summary: This study analyzed the transcriptional response of Prochlorococcus under different temperatures and found that the transcriptional suppression of photosynthetic machinery at low temperature may be one of the reasons for the restricted distribution of Prochlorococcus in temperate zones.
Article
Biochemical Research Methods
Yan Wang, Haitao Ge, Zhen Xiao, Chengcheng Huang, Gaojie Wang, Xiaoxiao Duan, Limin Zheng, Jinghui Dong, Xiahe Huang, Yuanya Zhang, Hongyu An, Wu Xu, Yingchun Wang
Summary: Spatial proteome reorganization is an important adaptation mechanism for photosynthetic organisms in addition to the differential expression of stress responsive genes. In this study, we treated a unicellular photosynthetic model cyanobacterium with different types of abiotic stresses and identified proteins showing stress induced differential expression and/or redistribution between cellular compartments. Our findings provide insights into the regulatory mechanism underlying stress responses and serve as a valuable resource for future studies on photosynthetic organisms' adaptation to adverse environments.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Biochemical Research Methods
Yan Wang, Haitao Ge, Zhen Xiao, Chengcheng Huang, Gaojie Wang, Xiaoxiao Duan, Limin Zheng, Jinghui Dong, Xiahe Huang, Yuanya Zhang, Hongyu An, Wu Xu, Yingchun Wang
Summary: This study identified proteins in a photosynthetic cyanobacterium that undergo differential expression and/or redistribution in response to different abiotic stresses. These findings provide insights into the regulatory mechanisms underlying stress responses and serve as an important resource for future studies on how photosynthetic organisms cope with adverse environments.
JOURNAL OF PROTEOME RESEARCH
(2023)
Article
Plant Sciences
Yuhua Yang, Xu Liu, Wenbin Zhang, Qian Qian, Limeng Zhou, Shu Liu, Yuge Li, Xingliang Hou
Summary: Under environmental stress conditions, asparagine-rich protein 1 and 2 inhibit cell death to promote plant survival.
Article
Plant Sciences
Shuai Lu, Cong Gao, Yongzhou Wang, Yingying He, Junrong Du, Moxian Chen, Hua Zhao, Hui Fang, Baohua Wang, Yunying Cao
Summary: This study identified U2AF65A in plant species, conducted phylogenetic and bioinformatic analyses, and studied the localization and stress responses of OsU2AF65A in rice. Using Arabidopsis and rice mutants, the importance of U2AF65A in plant growth and stress responses was demonstrated. These findings provide an overview of the U2AF65 gene family and its role in plant development and stress tolerance.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Multidisciplinary Sciences
Clara A. Fuchsman, David Garcia Prieto, Matthew D. Hays, Jacob A. Cram
Summary: This study analyzed the genomes of bacteria and viruses in ocean depths globally and found that environmental factors can lead to changes in the genetic composition of bacteria and viruses. The interaction between bacteria and viruses in different depths of the ocean is closely related to environmental factors.
Article
Plant Sciences
Runtian Miao, Mingyu Li, Zhenying Wen, Juan Meng, Xu Liu, Dongqing Fan, Wenjuan Lv, Tangren Cheng, Qixiang Zhang, Lidan Sun
Summary: In this study, we identified CDPK gene families in cultivated and wild mei. A total of 14 and 17 CDPK genes were found in the genomes of P. mume and P. mume var. Tortuosa, respectively. These genes were classified into four clades and showed high homologies with other Rosaceae species. The interaction network revealed their association with RBOHs and MAPK. RNA-seq data analysis showed that some cis-acting elements in the PmCDPK genes, especially PmCDPK14, were associated with cold hardiness. This study provides important insights into the role of CDPK gene families in cold stress response in plants.
Article
Biochemical Research Methods
Mingyi Zhang, Quan Luo, Huili Sun, Jacques Fritze, Guodong Luan, Xuefeng Lu
Summary: This study developed an inducible cyanobacteria cell factory using the ssrADAS-SspB-ClpXPEc system to achieve rapid degradation of target proteins. Additionally, an efficient and stringent inducible gene expression system was obtained, laying the foundation for future dynamic metabolic engineering efforts.
ACS SYNTHETIC BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hirotatsu Imai, Daisuke Utsumi, Hidetsugu Torihara, Kenzo Takahashi, Hidehito Kuroyanagi, Akio Yamashita
Summary: The coordinated regulation of dynamic RNA synthesis and translation is crucial for determining functional protein production. However, current reliable methods for simultaneously measuring nascent RNA synthesis and translation at the gene level are limited. In this study, a novel method combining 4-thiouridine (4sU) metabolic RNA labeling and translating ribosome affinity purification (TRAP) was developed to assess nascent RNA synthesis and translation simultaneously. This method may serve as a simple and powerful tool for analyzing the coordinated regulation of transcription and translation of individual genes in various eukaryotes.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Biochemistry & Molecular Biology
Abramo J. Manfredonia, Daniel A. Kraut
Summary: The ubiquitin-proteasome system is responsible for protein degradation in eukaryotic cells. The study showed that degradation of ubiquitin-independent degrons (UbIDs) is slower and relies on loosely folded substrates. Furthermore, UbID degradation is ATP-independent.
Article
Medical Laboratory Technology
Juan Luis Romero-Cabrera, Marta Garaulet, Jose Jimenez-Torres, Juan F. Alcala-Diaz, Gracia M. Quintana Navarro, Laura Martin-Piedra, Jose David Torres-Pena, Fernando Rodriguez-Cantalejo, Oriol Alberto Rangel-Zuniga, Elena Maria Yubero-Serrano, Raul M. Luque, Jose M. Ordovas, Jose Lopez-Miranda, Pablo Perez-Martinez, Antonio Garcia-Rios
Summary: Individuals with evening chronotypes have higher cardiometabolic risk and less robust circadian-related rhythms compared to morning-types, regardless of the nutritional intervention.
TRANSLATIONAL RESEARCH
(2022)
Article
Microbiology
Maria Agustina Dominguez-Martin, Antonio Lopez-Lozano, Yesica Melero-Rubio, Guadalupe Gomez-Baena, Juan Andres Jimenez-Estrada, Kateryna Kukil, Jesus Diez, Jose Manuel Garcia-Fernandeza
Summary: Marine Synechococcus and Prochlorococcus are important contributors to primary production on Earth, and their distribution is influenced by various factors. This study reveals a specific response of Synechococcus to nanomolar concentrations of nitrate, which may provide an evolutionary advantage for this organism over Prochlorococcus in the natural environment.
MICROBIOLOGY SPECTRUM
(2022)
Correction
Nutrition & Dietetics
Naomi Cano-Ibanez, Gracia M. Quintana-Navarro, Juan F. Alcala-Diaz, Oriol A. Rangel-Zuniga, Antonio Camargo, Elena M. Yubero-Serrano, Isabel Perez-Corral, Antonio P. Arenas-de Larriva, Antonio Garcia-Rios, Pablo Perez-Martinez, Javier Delgado-Lista, Jose Lopez-Miranda
EUROPEAN JOURNAL OF NUTRITION
(2022)
Review
Microbiology
J. Diez, A. Lopez-Lozano, M. A. Dominguez-Martin, G. Gomez-Baena, M. C. Munoz-Marin, Y. Melero-Rubio, J. M. Garcia-Fernandez
Summary: Marine picocyanobacteria, such as Prochlorococcus and Synechococcus, have developed unique adaptive mechanisms in nitrogen metabolism to thrive in oligotrophic oceans, including strategies such as reducing GC and protein contents, utilizing truncated proteins, and perceiving nanomolar nitrate concentrations. These adaptations contrast with the knowledge obtained in freshwater cyanobacteria and are essential for the ecological success of these microorganisms.
FEMS MICROBIOLOGY REVIEWS
(2023)
Article
Nutrition & Dietetics
Cristina Hidalgo-Moyano, Oriol Alberto Rangel-Zuniga, Francisco Gomez-Delgado, Juan F. Alcala-Diaz, Fernando Rodriguez-Cantalejo, Elena M. Yubero-Serrano, Jose D. Torres-Pena, Antonio P. Arenas-de Larriva, Antonio Camargo, Pablo Perez-Martinez, Jose Lopez-Miranda, Javier Delgado-Lista
Summary: This study investigated the interaction between long-term consumption of healthy diets and SIRT1 genotypes in modulating aging-related processes in patients with coronary heart disease. The results suggest that patients who are homozygous for the SIRT1-SNP rs7069102 may benefit from healthy diets, as it improves oxidative stress and inflammation, potentially slowing down the aging process and related diseases.
Article
Microbiology
Maria del Carmen Munoz-Marin, Solange Duhamel, Karin M. Bjorkman, Jonathan D. Magasin, Jesus Diez, David M. Karl, Jose M. Garcia-Fernandez
Summary: The marine cyanobacterium Prochlorococcus can assimilate glucose as a source of carbon, and its assimilation is influenced by photosynthetic light reactions. The timing of glucose assimilation in Prochlorococcus is different from that of other microbial populations. Transcriptional changes in specific genes indicate the importance of mixotrophic carbon assimilation by Prochlorococcus. This study provides insights into the ecological role of Prochlorococcus in the marine ecosystem.
MICROBIOLOGY SPECTRUM
(2022)
Article
Nutrition & Dietetics
Diana Maria Munoz-Perez, Clara Helena Gonzalez-Correa, Elcy Yaned Astudillo Munoz, Maite Sanchez-Giraldo, Juan Carlos Carmona-Hernandez, Jose Lopez-Miranda, Antonio Camargo, Oriol Alberto Rangel-Zuniga
Summary: Overweight and obesity are major global health issues. Healthy diets can induce molecular level changes. A study was conducted to evaluate the effect of a diet based on fruit, avocado, whole grains, and trout on obesity-related gene expression.
Article
Microbiology
Jose Angel Moreno-Cabezuelo, Guadalupe Gomez-Baena, Jesus Diez, Jose Manuel Garcia-Fernandez
Summary: This study compared the effects of different concentrations of glucose on the proteome and metabolome of Synechococcus sp. and Prochlorococcus sp. strains. The results showed that these cyanobacteria metabolize glucose primarily through oxidative pentoses and Calvin pathways, and no evidence was found for the involvement of the Entner-Doudoroff pathway. Differences in glucose effects were observed between genera and between Prochlorococcus MED4 and SS120 strains, possibly due to their specific adaptations to the environment. Additionally, fermentation was observed in Prochlorococcus sp. strain SS120 and Synechococcus sp. strain WH8102 after 5 mM glucose addition.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
M. Carmen Navarro-Ruiz, M. Carmen Soler-Vazquez, Alberto Diaz-Ruiz, Juan R. Peinado, Andrea Nieto Calonge, Julia Sanchez-Ceinos, Carmen Tercero-Alcazar, Jaime Lopez-Alcala, Oriol A. Rangel-Zuniga, Antonio Membrives, Jose Lopez-Miranda, Maria M. Malagon, Rocio Guzman-Ruiz
Summary: This study investigated the carbonylome of subcutaneous and omental human adipose tissue in obesity-associated insulin resistance. The results showed differences in carbonylated proteins between different fat depots, highlighting the complexity of oxidative stress at the metabolic level. Furthermore, the study revealed the altered expression and function of carbonylated transferrin, linking oxidative stress to metabolic dysfunction. This research provides insights into the molecular mechanisms underlying adipocyte dysfunction in obesity and identifies potential targets for therapeutic intervention.
Article
Biochemistry & Molecular Biology
Jose Angel Moreno-Cabezuelo, Maria del Carmen Munoz-Marin, Antonio Lopez-Lozano, Diogo Athayde, Ana Simon-Garcia, Jesus Diez, Margarida Archer, Federico M. Issoglio, Jose Manuel Garcia-Fernandez
Summary: The expression and mutation of Prochlorococcus marinus glcH gene in Escherichia coli were studied. Structural modeling and mutagenesis experiments showed that amino acid residues D8, N117, R141, and W348 play important roles in glucose transport. These studies provide insights into the high affinity and multiphasic kinetics of the glucose transporter, paving the way for further analysis.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2023)
