Article
Biochemistry & Molecular Biology
Aurelia Malapert, Emmanuelle Reboul, Olivier Dangles, Alain Thiery, N'nabinty Sylla, Valerie Tomao
Summary: A new simple and eco-friendly extraction process has been developed using native beta-cyclodextrin to remove phenolic compounds from olive mill waste water, achieving high extraction efficiency and loading capacity. The study found that the phenols encapsulated in solid beta-cyclodextrin are protected against photodegradation, making it a potential safe-grade food supplement.
Article
Biochemistry & Molecular Biology
Remi Rolland Ngoma Tchibouanga, Ayesha Jacobs
Summary: 3-chloro-4-hydroxyphenylacetic acid (CHPAA) is a fungal metabolite that is useful in crystal engineering studies. Salts formed with six selected amines were characterized using various techniques, revealing different contact types and thermal stabilities.
Article
Nutrition & Dietetics
Patricia Dias, Jana Pourova, Marie Voprsalova, Iveta Nejmanova, Premysl Mladenka
Summary: Regular intake of polyphenol-rich food can prevent cardiovascular diseases. A metabolite of polyphenols called 3-hydroxyphenylacetic acid (3-HPAA) decreases blood pressure by relaxing blood vessels, which might be mediated by the release of nitric oxide from endothelial cells.
Article
Biochemistry & Molecular Biology
Sheng An, Yi Yao, Junjie Wu, Hongbin Hu, Jie Wu, Maomao Sun, Jiaxin Li, Yaoyuan Zhang, Lulan Li, Weihuang Qiu, Yuying Li, Zhiya Deng, Haihong Fang, Shenhai Gong, Qiaobing Huang, Zhongqing Chen, Zhenhua Zeng
Summary: The increase of gut-derived 4-HPA in the kidney after sepsis could play a protective effect in SAKI by upregulating ARC to inhibit necroptosis.
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
(2024)
Article
Biochemistry & Molecular Biology
Francesca Annunziata, Martina L. Contente, Cecilia Pinna, Lucia Tamborini, Andrea Pinto
Summary: This study developed a sustainable biocatalyzed flow protocol for the selective oxidation of Ty into HTy by using free tyrosinase, and employed in-line extraction and purification methods to improve process efficiency and cost-effectiveness.
Article
Biochemistry & Molecular Biology
Enrique Almanza-Aguilera, Estefania Davila-Cordova, Daniel Guinon-Fort, Marta Farras, Giovanna Masala, Maria Santucci de Magistris, Ivan Baldassari, Rosario Tumino, Lisa Padroni, Verena A. Katzke, Matthias B. Schulze, Augustin Scalbert, Raul Zamora-Ros
Summary: This study analyzed the correlations between the acute and habitual intake of dietary tyrosols and their main food sources with urinary excretions of tyrosol and hydroxytyrosol. The results showed that urinary excretions correlated more strongly with acute intake than habitual intake. They also found correlations between urinary excretions and other tyrosol precursors as well as major food sources. Based on these findings, urinary excretions could be proposed as biomarkers of total tyrosol intake, especially for acute intakes.
Article
Pharmacology & Pharmacy
Maria Cristina Caroleo, Pierluigi Plastina, Alessia Fazio, Chiara La Torre, Fabrizio Manetti, Erika Cione
Summary: The study focused on the potential effects of natural lipophenols on insulin secretion in type 2 diabetes mellitus, and their role in modulating the FFAR1 receptor. The findings suggest that these compounds may play a role in the anti-diabetic properties associated with olive oil consumption.
Article
Food Science & Technology
Marina Gonzalez-Ramirez, Maria Mar Marin-Torres, Marta Gallardo-Fernandez, Andres Planells-Carcel, Ricardo Bisquert, Eva Valero, Cristina Ubeda, Ana Maria Troncoso, Maria Carmen Garcia-Parrilla
Summary: Yeasts are feasible and effective bioreactors, making them highly valuable in industrial production. This study investigated the production of bioactive compounds by Saccharomyces cerevisiae in fermented must. It was found that higher concentrations of hydroxytyrosol (HT) and melatonin (MEL) led to lower amounts of volatile compounds, especially higher alcohols and esters. The use of a modified yeast strain with increased HT production also resulted in higher levels of 2-phenylethanol (2-PE) and esters, but decreased levels of other important esters like ethyl hexanoate and ethyl octanoate. These findings suggest that S. cerevisiae may have different mechanisms for handling cell stress/toxicity related to nitrogen metabolism.
FOOD AND BIOPROCESS TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Africa Fernandez-Prior, Alejandra Bermudez-Oria, Juan Fernandez-Bolanos, Juan Antonio Espejo-Calvo, Francisco Lopez-Maestro, Guillermo Rodriguez-Gutierrez
Summary: The main by-product generated from the olive oil extraction process, alperujo, contains bioactive components, particularly phenolics. This study examined the evolution of four main phenolics in alperujo over two seasons and in ten oil mills. The results showed that the levels of hydroxytyrosol (HT) and tyrosol (Ty) increased, while 3,4-dihydroxyphenylglycol (DHPG) decreased towards the end of the season. Additionally, the study found that a mixture of alperujos from different mills can be a viable source of a liquid fraction rich in phenolic compounds.
Article
Biochemistry & Molecular Biology
Mario Abate, Marianna Citro, Simona Pisanti, Mariella Caputo, Rosanna Martinelli
Summary: The study revealed that 3-hydroxytyrosol promotes keratinocyte proliferation and migration, potentially aiding in wound healing and re-epithelialization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Xinmiao Wang, Qian Wang, Dong Cai, Xu Guo, Peiyong Tong, Fawen Yin, Xiaoyang Liu, Dayong Zhou
Summary: Hydroxytyrosol-SCFA acyl esters (HTy-SEs) and tyrosol-SCFA acyl esters (TYr-SEs) with different alkyl chain lengths (C1-C4) and isomers (branched-chain and straight-chain) were successfully synthesized and hydrolyzed to produce polyphenols and SCFAs. The hydrolysis rates were influenced by the carbon skeleton length and the esters with branched-chain fatty acids had weaker hydrolysis degrees. Additionally, the hydrolysis degrees of TYr-SEs were significantly higher than those of HTy-SEs. Controlled-release of polyphenols and SCFAs can be easily achieved by regulating the structures of polyphenols, carbon skeleton lengths, and isomers.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Microbiology
Zirun Jin, Yuzhuo Yang, Yalei Cao, Qi Wen, Yu Xi, Jianxing Cheng, Qiancheng Zhao, Jiaming Weng, Kai Hong, Hui Jiang, Jing Hang, Zhe Zhang
Summary: Our study explored the mechanism of how the microbiota affects male reproductive aging by utilizing fecal microbiota transplantation (FMT) and identified a gut bacteria-derived metabolite, 3-HPAA, that facilitates spermatogenesis in old mice through a ferroptosis-mediated mechanism. These findings provide a novel understanding of dysregulated spermatogenesis in aging males and suggest that 3-HPAA could be a potential therapy for fertility decline in clinical practice.
Article
Biochemistry & Molecular Biology
Yujia Liu, Conghui Shi, Gongliang Zhang, Honglei Zhan, Bingnan Liu, Cheng Li, Liang Wang, Han Wang, Jihui Wang
Summary: 4-HPCA inhibits the growth and proliferation of L. monocytogenes in a dose-dependent manner by affecting cell membrane structure and function, as well as decreasing the expression of virulence genes, which may lead to cell death.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Editorial Material
Biochemistry & Molecular Biology
Gloria Garrabou, Ana Sandra Hernandez, Mariona Guitart-Mampel, Elena Escalada-Casellas, Gemma Malats-Revelles, Sara Castro-Barquero, Ana Maria Ruiz-Leon, Kilian Vellve, Rosa Casas, Francesc Cardellach, Fatima Crispi, Francesc Josep Garcia-Garcia
Summary: This study found that dietary interventions, including supplementation with hydroxytyrosol, can improve complications of IUGR. Additionally, early biomarkers combined with dietary interventions may help predict IUGR diagnosis, aiding in avoiding risks of newborn mortality and morbidity.
Article
Food Science & Technology
Eugenia Papadaki, Fani Th Mantzouridou
Summary: The study tested the potential of utilizing Spanish-style green olive processing wastewaters for natural beta-carotene production by Blakeslea trispora, demonstrating higher beta-carotene yields in nutrient-enriched effluents. However, the low sugar and nitrogen content in the wastewaters were found to be unfavorable for carotenogenesis.
Article
Biotechnology & Applied Microbiology
Celine Boileau, Richard Auria, Sylvain Davidson, Laurence Casalot, Pierre Christen, Pierre-Pol Liebgott, Yannick Combet-Blanc
BIOTECHNOLOGY FOR BIOFUELS
(2016)
Article
Biotechnology & Applied Microbiology
Richard Auria, Celine Boileau, Sylvain Davidson, Laurence Casalot, Pierre Christen, Pierre Pol Liebgott, Yannick Combet-Blanc
BIOTECHNOLOGY FOR BIOFUELS
(2016)
Article
Microbiology
Danielle Moinier, Deborah Byrne, Agnes Amouric, Violaine Bonnefoy
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Agricultural Engineering
Guillaume Pillot, Eleonore Frouin, Emilie Pasero, Anne Godfroy, Yannick Combet-Blanc, Sylvain Davidson, Pierre-Pol Liebgott
BIORESOURCE TECHNOLOGY
(2018)
Article
Chemistry, Medicinal
Laurent Hoffer, Yuliia V. Voitovich, Brigitt Raux, Kendall Carrasco, Christophe Muller, Aleksey Y. Fedorov, Carine Derviaux, Agnes Amouric, Stephane Betzi, Dragos Horvath, Alexandre Varnek, Yves Collette, Sebastien Combes, Philippe Roche, Xavier Morelli
JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Hana Gannoun, Lamia Ben Gaida, Rafika Saidi, Baligh Miladi, Moktar Hamdi, Hassib Bouallagui, Pierre Pol Liebgott, Richard Auria
PROCESS BIOCHEMISTRY
(2018)
Article
Engineering, Environmental
Rafika Saidi, Pierre Pol Liebgott, Hana Gannoun, Lamia Ben Gaida, Baligh Miladi, Moktar Hamdi, Hassib Bouallagui, Richard Auria
Article
Ecology
Guillaume Pillot, Sylvain Davidson, Richard Auria, Yannick Combet-Blanc, Anne Godfroy, Pierre-Pol Liebgott
Article
Biochemistry & Molecular Biology
Clarisse Roblin, Steve Chiumento, Cedric Jacqueline, Eric Pinloche, Cendrine Nicoletti, Hamza Olleik, Elise Courvoisier-Dezord, Agnes Amouric, Christian Basset, Louis Dru, Marie Ollivier, Aurelie Bogey-Lambert, Nicolas Vidal, Mohamed Atta, Marc Maresca, Estelle Devillard, Victor Duarte, Josette Perrier, Mickael Lafond
Summary: The research indicates that ruminococcin C1, a RiPPs molecule, has strong antibacterial activity and safety, effectively treating infections and protecting challenged mice from resistant bacteria. It selectively impacts the gut microbial community while maintaining global microbiome homeostasis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Faustine Lorquin, Fabio Ziarelli, Agnes Amouric, Carole Di Giorgio, Maxime Robin, Philippe Piccerelle, Jean Lorquin
Summary: Various production methods for pyomelanin polymer were evaluated in this study, with the most promising one involving a recombinant laccase extract showing high stability, non-cytotoxicity, and strong antioxidant properties. The chemical structures of the polymers were investigated, indicating the presence of primarily C-3-C-6 and C-4-C-6 configurations. These findings suggest that pyomelanin produced by enzymatic processes may have application potential in extreme conditions and skin protection.
SCIENTIFIC REPORTS
(2021)
Article
Multidisciplinary Sciences
Guillaume Pillot, Oulfat Amin Ali, Sylvain Davidson, Laetitia Shintu, Anne Godfroy, Yannick Combet-Blanc, Patricia Bonin, Pierre-Pol Liebgott
Summary: Deep-sea hydrothermal vents are extreme and complex ecosystems, with the first colonizers likely being (hyper)thermophilic autotrophs. Experiments have shown that utilizing electric potential as an energy source can support the growth of electrotrophic biofilms, driving microbial colonization.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Physical
Elif Erdem, Lenny Malihan-Yap, Leen Assil-Companioni, Hanna Grimm, Giovanni Davide Barone, Carole Serveau-Avesque, Agnes Amouric, Katia Duquesne, Veronique de Berardinis, Yagut Allahverdiyeva, Veronique Alphand, Robert Kourist
Summary: BVMOXeno is a highly efficient Baeyer-Villiger monooxygenase with higher reaction rates and lower KM value compared to currently known BVMOs, almost completely suppressing unwanted side reactions. The recombinant production of BVMO in cyanobacteria allows more efficient utilization of water and oxygen generated by photosynthesis.
Article
Microbiology
Guillaume Pillot, Oulfat Amin Ali, Sylvain Davidson, Laetitia Shintu, Yannick Combet-Blanc, Anne Godfroy, Patricia Bonin, Pierre-Pol Liebgott
Summary: Recent studies have shown the presence of an abiotic electrical current across deep-sea hydrothermal chimneys, supporting the growth of electroautotrophic microbial communities. The study focused on electrotrophic enrichment with nitrate as an electron acceptor, monitoring biofilm density, community composition, organic compound production, and electrical consumption. Archaeoglobales were found to play a key role in community development, with observed increase in biodiversity over time.
Review
Biology
Guillaume Pillot, Oscar Santiago, Sven Kerzenmacher, Pierre-Pol Liebgott
Summary: The emergence of life has been extensively studied, with recent attention given to the discovery of natural electrical current in deep-sea hydrothermal vents. This new energy source, known as electrotrophy, has implications for understanding the transition from inorganic to organic life forms. In this review, the role of electrochemical reactions in each step of life's formation is explored, including the evaluation of electrical current in the Hadean era, CO2 electroreduction into a prebiotic primordial soup, proto-membrane production, nitrate reduction inspired energetic system, proton gradient, and the transition to a planktonic proto-cell. The theory is compared to other hydrothermal theories to assess its relevance and ability to overcome limitations.
Article
Chemistry, Multidisciplinary
Fangfang Yang, Pierre Rousselot-Pailley, Cendrine Nicoletti, A. Jalila Simaan, Bruno Faure, Elise Courvoisier-Dezord, Agnes Amouric, Yolande Charmasson, Renal Backov, Thierry Tron, Yasmina Mekmouche
Summary: This study investigates the site-directed immobilization of a hybrid catalyst bearing a biquinoline-based-Pd(II) complex and a robust laccase within cavities of a silica foam to favor veratryl alcohol oxidation. The catalytic activity is dependent on the orientation and loading of each hybrid, and the system can be reused multiple times with a high operational activity remaining. The work demonstrates the concept of controlling the organization of a heterogeneous hybrid catalyst using a Pd/laccase/silica foam.