期刊
RESEARCH IN MICROBIOLOGY
卷 159, 期 9-10, 页码 643-650出版社
ELSEVIER SCIENCE BV
DOI: 10.1016/j.resmic.2008.08.006
关键词
Glucosamine-inositol; Gene knock-out; Growth effects; Ergothioneine; Rhodococcus jostii
类别
资金
- Natural Sciences and Engineering Research Council of Canada
- National Institutes of Health [A1072133]
Mycothiol (1D-myo-inosityl 2-[N-acetyl-L-Cysteinyl]amido-2-deoxy-alpha-D-glucopyranoside) is an important microbial thiol present only in actinomycetes. Rhodococcus jostii RHA1 degrades a wide range of xenobiotics, including polychlorinated biphenyls, nitriles, and N-nitrosodimethylamine. Analyses revealed that this strain produces two thiols, mycothiol and ergothioneine, found in the other actinomycetes. A mycothiol ligase mutant strain of R. jostii RHA1 deficient in the production of mycothiol was constructed. This mutant has a number of interesting characteristics: (a) it overproduces the intermediate glucosamine-inositol (1-O-(2-amino-1-deoxy-alpha-D-glucopyranosyl)-D-myo-inositol); (b) it is deficient in the biochemical degradation of a number of xenobiotics metabolized by the parent strain; (c) it shows increased susceptibility to a number of antibiotics; and (d) it shows unusual growth characteristics, exhibiting a long tag phase before normal exponential growth. The diverse phenotypes of the mutant indicate the utility of R. jostii RHA1 as a model for deciphering the various functions of mycothiol. (C) 2008 Elsevier Masson SAS. All rights reserved.
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